##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139100_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9684730 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.571789404557485 33.0 33.0 33.0 33.0 33.0 2 32.57722641725686 33.0 33.0 33.0 33.0 33.0 3 32.57077667627286 33.0 33.0 33.0 33.0 33.0 4 36.47126837815819 37.0 37.0 37.0 37.0 37.0 5 36.500930330530636 37.0 37.0 37.0 37.0 37.0 6 36.49804279520441 37.0 37.0 37.0 37.0 37.0 7 36.476053333443474 37.0 37.0 37.0 37.0 37.0 8 36.45768947611343 37.0 37.0 37.0 37.0 37.0 9 36.49610345358105 37.0 37.0 37.0 37.0 37.0 10-11 36.45073146076349 37.0 37.0 37.0 37.0 37.0 12-13 36.382286599626426 37.0 37.0 37.0 37.0 37.0 14-15 38.421752645659716 40.0 37.0 40.0 37.0 40.0 16-17 38.38059641311632 40.0 37.0 40.0 37.0 40.0 18-19 38.38852977832113 40.0 37.0 40.0 37.0 40.0 20-21 38.383589235282464 40.0 37.0 40.0 37.0 40.0 22-23 38.37958798670461 40.0 37.0 40.0 37.0 40.0 24-25 38.37977311990108 40.0 37.0 40.0 37.0 40.0 26-27 38.233263747593455 40.0 37.0 40.0 37.0 40.0 28-29 38.15698823330126 40.0 37.0 40.0 35.0 40.0 30-31 38.12290706598935 40.0 37.0 40.0 35.0 40.0 32-33 38.047521734599854 40.0 37.0 40.0 33.0 40.0 34-35 38.011809998224564 40.0 37.0 40.0 33.0 40.0 36-37 37.97423034431913 40.0 37.0 40.0 33.0 40.0 38-39 37.929236227142184 40.0 37.0 40.0 33.0 40.0 40-41 37.89696426178983 40.0 37.0 40.0 33.0 40.0 42-43 37.80101981573537 40.0 37.0 40.0 33.0 40.0 44-45 37.75395888245972 40.0 37.0 40.0 33.0 40.0 46-47 37.71704791460368 40.0 37.0 40.0 33.0 40.0 48-49 37.69014596856694 40.0 37.0 40.0 33.0 40.0 50-51 37.629950348178 40.0 37.0 40.0 33.0 40.0 52-53 37.52748837584632 40.0 37.0 40.0 33.0 40.0 54-55 37.31659714409274 40.0 37.0 40.0 33.0 40.0 56-57 37.13479368604274 38.5 37.0 40.0 33.0 40.0 58-59 37.06954921316648 37.0 37.0 40.0 33.0 40.0 60-61 36.93659019954434 37.0 37.0 40.0 33.0 40.0 62-63 36.750893803298965 37.0 37.0 40.0 33.0 40.0 64-65 36.60078082509817 37.0 37.0 40.0 33.0 40.0 66-67 36.381594405748544 37.0 37.0 40.0 33.0 40.0 68-69 36.15932997198274 37.0 37.0 40.0 33.0 40.0 70-71 35.90019357078479 37.0 35.0 37.0 33.0 40.0 72-73 35.69155893584498 37.0 33.0 37.0 33.0 40.0 74-75 35.513450805004105 37.0 33.0 37.0 33.0 40.0 76-77 33.72266670334763 35.0 33.0 37.0 27.0 37.0 78-79 34.96274996054805 37.0 33.0 37.0 33.0 37.0 80-81 35.035581436748366 37.0 33.0 37.0 33.0 37.0 82-83 34.925605218755045 37.0 33.0 37.0 33.0 37.0 84-85 34.77615221238521 37.0 33.0 37.0 33.0 37.0 86-87 34.65071318033449 37.0 33.0 37.0 33.0 37.0 88-89 34.57568810149171 37.0 33.0 37.0 33.0 37.0 90-91 34.49346436508694 37.0 33.0 37.0 33.0 37.0 92-93 34.42603596241415 37.0 33.0 37.0 33.0 37.0 94-95 34.414392590923384 37.0 33.0 37.0 33.0 37.0 96-97 34.41978389886104 37.0 33.0 37.0 33.0 37.0 98-99 34.42862578167281 37.0 33.0 37.0 33.0 37.0 100 34.4338311049058 37.0 33.0 37.0 33.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 15.0 20 89.0 21 425.0 22 1560.0 23 4483.0 24 10608.0 25 20416.0 26 36023.0 27 56680.0 28 81982.0 29 115668.0 30 155911.0 31 205343.0 32 270319.0 33 358927.0 34 488532.0 35 705027.0 36 1205858.0 37 2765449.0 38 3132907.0 39 68506.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.68125492398859 20.084153094613892 14.345748410126044 23.888843571271476 2 14.58250255815082 20.779980443440344 41.970700267328056 22.666816731080782 3 18.317836429100243 26.033012794368044 30.111278270018886 25.537872506512834 4 12.491984804945517 17.960717541944895 38.791055610223516 30.75624204288607 5 13.540356829772229 37.66692514917814 35.40123472724588 13.391483293803752 6 30.24043628277428 37.71209681882651 18.71480541322379 13.332661485175423 7 26.74375021296412 32.47626934359554 23.007414765305796 17.772565678134548 8 24.36092694375579 37.153715178430375 20.860178858884037 17.6251790189298 9 25.664866237881697 16.880573851826536 20.99602157210371 36.458538338188056 10-11 24.43037313968771 26.75725653510352 28.661462129914273 20.1509081952945 12-13 25.274560462931944 24.164449382126346 28.833857794244977 21.727132360696732 14-15 22.310301606649478 25.555374615706477 26.436131170536488 25.698192607107558 16-17 21.949563901110306 28.686034613251998 27.105980238994785 22.25842124664291 18-19 21.953312069618875 27.843739577665044 28.799920080373948 21.40302827234213 20-21 22.750038825102827 27.01516223523552 28.702680873350033 21.53211806631162 22-23 22.47673886002278 26.925604367796236 28.553196153926187 22.044460618254803 24-25 22.259289148517464 27.061734377886303 28.591980623084822 22.08699585051141 26-27 22.207550452475036 27.228653418750792 28.4825008409779 22.081295287796273 28-29 22.126354335371833 27.314795962134937 28.510192332934732 22.048657369558498 30-31 22.395465232375965 27.15679643679394 28.424738473670292 22.022999857159807 32-33 22.203929156478996 27.262957655370933 28.419413863449567 22.113699324700505 34-35 22.327318067771166 27.338558261302264 28.349477788261996 21.984645882664573 36-37 22.354189340792377 27.27369876336951 28.25683168513608 22.11528021070204 38-39 22.1806470724145 27.380307020347555 28.342957487851255 22.096088419386685 40-41 22.42669659941975 27.286435609064057 28.187492478252114 22.099375313264076 42-43 22.326430747348734 27.398635623940738 27.971712796857034 22.303220831853494 44-45 22.402830015901518 27.447163479607628 27.856962320004396 22.29304418448646 46-47 22.50520846776329 27.51921536778406 27.567277500354425 22.408298664098222 48-49 22.427532238984334 27.637858356823436 27.513851915053355 22.420757489138875 50-51 22.42414712301012 27.759001810560967 27.424023393853407 22.392827672575503 52-53 22.622589516678012 27.601955071477196 27.32839210347175 22.447063308373043 54-55 22.573731387429035 27.5180189679112 27.372158305102385 22.53609133955738 56-57 22.538925972266753 27.693828741320033 27.32526393004952 22.4419813563637 58-59 22.5257521889319 27.66330264673441 27.34616784771849 22.4647773166152 60-61 22.573646036250313 27.611684845850064 27.394399207863856 22.42026991003576 62-63 22.554491100243297 27.6947292989029 27.314371338619615 22.436408262234192 64-65 22.551717799167832 27.663075719582626 27.268765031216713 22.516441450032833 66-67 22.557257538992904 27.673304835288814 27.177254857610862 22.592182768107424 68-69 22.515443450142524 27.63935579161116 27.269825015396638 22.575375742849683 70-71 22.52859060258129 27.49211734460948 27.31126658814264 22.66802546466659 72-73 22.575974649165055 27.5129874237989 27.225855227664237 22.685182699371808 74-75 22.454812945627182 27.66320136456013 27.245543941411494 22.636441748401197 76-77 22.612896873060336 27.561661420894374 27.16551997367557 22.659921732369725 78-79 22.561168560115917 27.609410496193888 27.11244883286063 22.716972110829566 80-81 22.56948741463694 27.650732831554787 27.123048978949786 22.656730774858488 82-83 22.5772940469735 27.58115759220743 27.212403393098917 22.62914496772015 84-85 22.638223580159174 27.57366076243945 27.199804700956637 22.588310956444737 86-87 22.531137058151153 27.59625528252435 27.2626868221938 22.609920837130698 88-89 22.559634206344978 27.547440039863737 27.23410229811932 22.658823455671964 90-91 22.613821539144944 27.620221057203427 27.183886246791683 22.582071156859946 92-93 22.63049896908063 27.555559233820777 27.2090153254065 22.604926471692092 94-95 22.504629423761887 27.60629211566421 27.26129895350709 22.627779507066812 96-97 22.608040827535227 27.68754771178631 27.29114508353428 22.413266377144183 98-99 23.69464083692104 29.649877734716696 27.470863295590885 19.184618132771384 100 22.611938319006654 30.656823967788625 21.29308780541032 25.4381499077944 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1803.0 1 1626.0 2 2163.5 3 3095.0 4 4283.0 5 7219.0 6 9664.0 7 11033.5 8 11764.5 9 11331.5 10 10350.0 11 9499.0 12 10480.0 13 13872.5 14 21957.0 15 33664.5 16 43599.5 17 49680.5 18 51315.5 19 48792.0 20 43782.0 21 38112.5 22 34382.5 23 35243.0 24 40595.5 25 49997.5 26 63299.0 27 79175.0 28 97505.5 29 118560.5 30 140834.0 31 164054.5 32 187550.0 33 210471.5 34 233461.5 35 254860.5 36 276790.0 37 299061.5 38 317524.5 39 331802.0 40 347386.5 41 362290.0 42 371084.5 43 378092.5 44 384495.0 45 391728.5 46 396300.0 47 397163.0 48 398105.5 49 394133.0 50 385292.0 51 374364.0 52 360755.0 53 343980.0 54 325205.0 55 303709.5 56 280643.0 57 254652.0 58 225689.5 59 196788.5 60 165726.5 61 135667.0 62 109136.5 63 84903.0 64 63285.5 65 46556.5 66 34642.5 67 26226.0 68 19943.5 69 14478.0 70 10202.0 71 7187.5 72 4908.0 73 3254.5 74 2209.0 75 1471.5 76 934.5 77 580.5 78 377.5 79 247.0 80 171.0 81 130.0 82 106.5 83 84.5 84 62.0 85 41.5 86 33.5 87 30.0 88 21.0 89 12.5 90 8.0 91 6.0 92 5.0 93 4.5 94 2.5 95 1.0 96 1.0 97 0.5 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 2.0651066162918324E-5 7 0.0 8 0.0 9 0.0 10-11 0.0017192012580629504 12-13 2.4265002741429034E-4 14-15 0.007496337017139352 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 165.0 20-21 600.0 22-23 1211.0 24-25 2742.0 26-27 3431.0 28-29 5471.0 30-31 13024.0 32-33 16753.0 34-35 23284.0 36-37 33864.0 38-39 42513.0 40-41 45659.0 42-43 55525.0 44-45 67327.0 46-47 65757.0 48-49 59912.0 50-51 53477.0 52-53 51976.0 54-55 55734.0 56-57 58818.0 58-59 59915.0 60-61 59686.0 62-63 58280.0 64-65 61490.0 66-67 66320.0 68-69 68825.0 70-71 69091.0 72-73 67095.0 74-75 68688.0 76-77 67829.0 78-79 74331.0 80-81 77115.0 82-83 74871.0 84-85 75685.0 86-87 79689.0 88-89 87437.0 90-91 93319.0 92-93 99244.0 94-95 120444.0 96-97 343805.0 98-99 619156.0 100-101 6635172.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.42823677380818 #Duplication Level Percentage of deduplicated Percentage of total 1 88.46466328775831 73.80450874886317 2 7.98969357875656 13.331320952773543 3 1.96533459994772 4.91893201032588 4 0.733203177357593 2.44679393135591 5 0.3347137970843171 1.3962290957305397 6 0.17116408007333134 0.856795043971737 7 0.09765862091536476 0.5703240584111439 8 0.06421918245382496 0.4286154527342477 9 0.040991043347169985 0.3077829422975819 >10 0.13511165479602164 1.7115595929961833 >50 0.0027205191592728326 0.1493912001343076 >100 5.264583505582476E-4 0.07774697040568308 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.0325533081459164E-5 0.0 1.0325533081459164E-5 0.0 3 0.0 2.0651066162918327E-5 0.0 1.0325533081459164E-5 0.0 4 0.0 5.162766540729582E-5 0.0 2.0651066162918327E-5 0.0 5 0.0 5.162766540729582E-5 0.0 2.0651066162918327E-5 0.0 6 7.227873157021414E-5 5.162766540729582E-5 0.0 2.0651066162918327E-5 0.0 7 7.227873157021414E-5 5.162766540729582E-5 0.0 3.097659924437749E-5 0.0 8 7.227873157021414E-5 6.195319848875498E-5 0.0 3.097659924437749E-5 3.097659924437749E-5 9 7.227873157021414E-5 1.135808638960508E-4 0.0 4.1302132325836654E-5 3.097659924437749E-5 10-11 7.227873157021414E-5 1.135808638960508E-4 0.0 6.711596502948457E-5 3.097659924437749E-5 12-13 7.227873157021414E-5 1.1874363043678037E-4 0.0 7.227873157021414E-5 3.613936578510707E-5 14-15 9.292979773313247E-5 2.323244943328312E-4 0.0 7.227873157021414E-5 5.162766540729582E-5 16-17 1.445574631404283E-4 2.5297556049574954E-4 0.0 7.227873157021414E-5 6.195319848875498E-5 18-19 1.445574631404283E-4 2.6330109357720866E-4 0.0 7.227873157021414E-5 6.195319848875498E-5 20-21 1.445574631404283E-4 2.787893931993974E-4 0.0 7.227873157021414E-5 6.195319848875498E-5 22-23 1.445574631404283E-4 3.8720749055471857E-4 0.0 7.744149811094372E-5 7.744149811094372E-5 24-25 1.445574631404283E-4 4.5432345558420314E-4 0.0 8.77670311924029E-5 9.292979773313247E-5 26-27 1.445574631404283E-4 4.853000548285807E-4 0.0 1.0841809735532122E-4 9.292979773313247E-5 28-29 1.445574631404283E-4 4.904628213693102E-4 0.0 1.9102236200699453E-4 9.292979773313247E-5 30-31 1.445574631404283E-4 5.369277202358765E-4 0.0 6.814851833763048E-4 9.809256427386205E-5 32-33 1.445574631404283E-4 5.369277202358765E-4 0.0 0.001677899125737114 1.135808638960508E-4 34-35 1.6520852930334662E-4 6.143692183468203E-4 0.0 0.00308733439135629 1.342319300589691E-4 36-37 1.6520852930334662E-4 7.021362495392232E-4 0.0 0.004971744178722587 1.445574631404283E-4 38-39 1.6520852930334662E-4 7.227873157021414E-4 0.0 0.008275914764789519 1.445574631404283E-4 40-41 1.6520852930334662E-4 8.570192457611106E-4 0.0 0.011838223677892931 1.445574631404283E-4 42-43 1.6520852930334662E-4 8.931586115462175E-4 0.0 0.014966860201575057 1.445574631404283E-4 44-45 1.6520852930334662E-4 9.706001096571613E-4 0.0 0.0183949371846195 1.445574631404283E-4 46-47 1.6520852930334662E-4 0.001027390541605187 0.0 0.022349616354818358 1.6520852930334662E-4 48-49 1.6520852930334662E-4 0.0010325533081459164 0.0 0.026366248723505972 1.7553406238480577E-4 50-51 1.8585959546626494E-4 0.001156459705123426 0.0 0.030336416193327023 1.7553406238480577E-4 52-53 1.8585959546626494E-4 0.0011822735378270743 0.0 0.03444081559320704 1.8585959546626492E-4 54-55 1.8585959546626494E-4 0.0011925990709085333 0.0 0.038240611767184005 2.0134789508845368E-4 56-57 1.8585959546626494E-4 0.001208087370530722 0.0 0.04213850050543484 2.0651066162918327E-4 58-59 1.8585959546626494E-4 0.0012184129036121813 0.0 0.046351318002670186 2.0651066162918327E-4 60-61 1.961851285477241E-4 0.001270040569019477 0.0 0.051178504718252346 2.1167342816991283E-4 62-63 2.3748726087356075E-4 0.0012803661021009362 0.0 0.055365508382784034 2.271617277921016E-4 64-65 2.3748726087356075E-4 0.0013991097325377166 0.0 0.05941311735071603 2.271617277921016E-4 66-67 2.478127939550199E-4 0.0014145980321599054 0.0 0.06374984124492887 2.271617277921016E-4 68-69 2.478127939550199E-4 0.0014817139971893898 0.0 0.06812786727146755 2.271617277921016E-4 70-71 2.581383270364791E-4 0.0014972022968115786 0.0 0.07231487093599925 2.3748726087356075E-4 72-73 2.787893931993974E-4 0.0014972022968115786 0.0 0.0757790872848288 2.684638601179382E-4 74-75 2.787893931993974E-4 0.0015643182618410632 0.0 0.07849470248525256 2.684638601179382E-4 76-77 2.83952159740127E-4 0.0016624108261149252 0.0 0.08082827296166233 2.684638601179382E-4 78-79 2.891149262808566E-4 0.0017140384915222211 0.0 0.08238742845696267 2.684638601179382E-4 80-81 2.891149262808566E-4 0.0017192012580629506 0.0 0.0828675657452505 2.787893931993974E-4 82-83 2.9427769282158614E-4 0.0017295267911444099 0.0 0.08331156366775325 2.891149262808566E-4 84-85 2.994404593623157E-4 0.0017450150907665986 0.0 0.08349742326321952 2.891149262808566E-4 86-87 2.994404593623157E-4 0.0017450150907665986 0.0 0.08358002752787119 2.891149262808566E-4 88 2.994404593623157E-4 0.001837944888499731 0.0 0.08359551582749339 2.891149262808566E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12825 0.0 48.357483 1 GTATCAA 21925 0.0 35.89314 1 TCAACGC 26325 0.0 29.332983 4 ATCAACG 26745 0.0 28.88858 3 CAACGCA 27270 0.0 28.364857 5 TATCAAC 27870 0.0 27.831547 2 AACGCAG 28045 0.0 27.627478 6 ACGCAGA 32380 0.0 23.915325 7 CGCAGAG 32955 0.0 23.472057 8 GTACATG 30025 0.0 22.246729 1 TACATGG 30035 0.0 21.516327 2 ACATGGG 30295 0.0 20.786907 3 GCAGAGT 39160 0.0 19.666256 9 GAGTACT 21660 0.0 19.391647 12-13 CATGGGG 22220 0.0 18.84195 4 GTGGTAT 6640 0.0 18.771833 1 CAGAGTA 37575 0.0 18.31677 10-11 AGAGTAC 34905 0.0 18.087688 10-11 TGGTATC 6375 0.0 17.644625 2 GTACTTT 23790 0.0 17.601036 14-15 >>END_MODULE