FastQCFastQC Report
Fri 27 May 2016
SRR2139099_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139099_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9498693
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137600.052.1673161
GTATCAA252700.038.1190531
TCAACGC304750.030.9302464
ATCAACG307150.030.7025853
CAACGCA313550.030.0770115
TATCAAC318000.029.939392
AACGCAG318800.029.5952076
ACGCAGA363900.025.8681557
CGCAGAG368800.025.5130548
GCAGAGT426750.021.9006359
GTACATG334050.021.527141
TACATGG337600.020.701292
GAGTACT240400.020.32471812-13
ACATGGG346450.019.8120353
GTGGTAT64400.019.7918851
CAGAGTA419300.019.52151710-11
TGGTATC62750.019.4888382
AGAGTAC393300.018.72042310-11
GTACTTT266050.018.40601214-15
CATGGGG249000.018.0543754
Produced by FastQC (version 0.11.5)