##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139099_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9498693
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57342383841651	33.0	33.0	33.0	33.0	33.0
2	32.582543093033955	33.0	33.0	33.0	33.0	33.0
3	32.57765663128601	33.0	33.0	33.0	33.0	33.0
4	36.47902632499019	37.0	37.0	37.0	37.0	37.0
5	36.50965643378515	37.0	37.0	37.0	37.0	37.0
6	36.5081430676831	37.0	37.0	37.0	37.0	37.0
7	36.48432126398863	37.0	37.0	37.0	37.0	37.0
8	36.467913532946056	37.0	37.0	37.0	37.0	37.0
9	36.50024387565742	37.0	37.0	37.0	37.0	37.0
10-11	36.45789083824479	37.0	37.0	37.0	37.0	37.0
12-13	36.38538454711611	37.0	37.0	37.0	37.0	37.0
14-15	38.42097718075529	40.0	37.0	40.0	37.0	40.0
16-17	38.38381017262059	40.0	37.0	40.0	37.0	40.0
18-19	38.39929309221806	40.0	37.0	40.0	37.0	40.0
20-21	38.39868882331022	40.0	37.0	40.0	37.0	40.0
22-23	38.39523268685798	40.0	37.0	40.0	37.0	40.0
24-25	38.394542594792966	40.0	37.0	40.0	37.0	40.0
26-27	38.245804484954235	40.0	37.0	40.0	37.0	40.0
28-29	38.17029611845623	40.0	37.0	40.0	37.0	40.0
30-31	38.132520703605834	40.0	37.0	40.0	37.0	40.0
32-33	38.05449057021399	40.0	37.0	40.0	33.0	40.0
34-35	38.020625036483	40.0	37.0	40.0	33.0	40.0
36-37	37.98290159767452	40.0	37.0	40.0	33.0	40.0
38-39	37.94087748527387	40.0	37.0	40.0	33.0	40.0
40-41	37.90968086159154	40.0	37.0	40.0	33.0	40.0
42-43	37.81326359286753	40.0	37.0	40.0	33.0	40.0
44-45	37.76722386355169	40.0	37.0	40.0	33.0	40.0
46-47	37.73026516690015	40.0	37.0	40.0	33.0	40.0
48-49	37.704505005551184	40.0	37.0	40.0	33.0	40.0
50-51	37.648797215721515	40.0	37.0	40.0	33.0	40.0
52-53	37.548062103212615	40.0	37.0	40.0	33.0	40.0
54-55	37.34399114726075	40.0	37.0	40.0	33.0	40.0
56-57	37.196515986086666	40.0	37.0	40.0	33.0	40.0
58-59	37.10933217248322	37.0	37.0	40.0	33.0	40.0
60-61	36.97331492063962	37.0	37.0	40.0	33.0	40.0
62-63	36.78849713746823	37.0	37.0	40.0	33.0	40.0
64-65	36.640978672936306	37.0	37.0	40.0	33.0	40.0
66-67	36.42466446030019	37.0	37.0	40.0	33.0	40.0
68-69	36.208610658842986	37.0	37.0	40.0	33.0	40.0
70-71	35.949896996794756	37.0	37.0	38.5	33.0	40.0
72-73	35.743214077922794	37.0	33.0	37.0	33.0	40.0
74-75	35.56887989707366	37.0	33.0	37.0	33.0	40.0
76-77	33.780860250196795	35.0	33.0	37.0	27.0	37.0
78-79	35.009043859160926	37.0	33.0	37.0	33.0	37.0
80-81	35.07774709776049	37.0	33.0	37.0	33.0	37.0
82-83	34.96665984306347	37.0	33.0	37.0	33.0	37.0
84-85	34.81663256462796	37.0	33.0	37.0	33.0	37.0
86-87	34.695607177371635	37.0	33.0	37.0	33.0	37.0
88-89	34.62472377316672	37.0	33.0	37.0	33.0	37.0
90-91	34.54545541072274	37.0	33.0	37.0	33.0	37.0
92-93	34.47593506265413	37.0	33.0	37.0	33.0	37.0
94-95	34.468960359684004	37.0	33.0	37.0	33.0	37.0
96-97	34.479726858395765	37.0	33.0	37.0	33.0	37.0
98-99	34.48418552120237	37.0	33.0	37.0	33.0	37.0
100	34.48606717663301	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	13.0
20	101.0
21	472.0
22	1517.0
23	4403.0
24	9883.0
25	19293.0
26	34030.0
27	53343.0
28	78646.0
29	110259.0
30	149504.0
31	197155.0
32	260481.0
33	346915.0
34	475704.0
35	680392.0
36	1161135.0
37	2694492.0
38	3146089.0
39	74863.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.897185223272295	20.111082651055256	14.33204547194019	23.659686653732255
2	14.657016496901203	20.838719600686115	41.946307770974386	22.557956131438296
3	18.305528981724116	26.204394646716132	30.18144706855985	25.308629302999897
4	12.500425058479097	18.01075158445483	38.76185913156684	30.726964225499238
5	13.407286665649684	37.836952936577696	35.31763791081573	13.43812248695689
6	30.050993342135246	37.7481802492575	18.758153097095168	13.442673311512083
7	26.693272432323056	32.66732591526013	22.95929555782043	17.680106094596383
8	24.446226443996032	36.93695543165781	20.84514153684091	17.77167658750525
9	25.824952969845434	16.81897709505929	21.054380850081163	36.30168908501412
10-11	24.50434240282957	26.77753840773264	28.563928172984003	20.154191016453787
12-13	25.40750735129776	24.34736341314562	28.719183952520478	21.525945283036148
14-15	22.395158848379257	25.62383212107659	26.574796683255443	25.406212347288708
16-17	21.822054886919705	28.754329674619445	27.170464399681094	22.253151038779755
18-19	21.86927717318583	27.799782559558455	29.056792339746114	21.274147927509606
20-21	22.757263586187374	26.898387200951063	28.969651756457083	21.374697456404483
22-23	22.464715678875894	26.87695689494289	28.816643665405216	21.841683760776
24-25	22.176295861783167	27.056268143434032	28.86048308069652	21.90695291408628
26-27	22.100348636008498	27.165684701229942	28.790784471053477	21.943182191708086
28-29	22.006345241200854	27.287567490652098	28.7962908797698	21.90979638837725
30-31	22.312105677089463	27.149254699698755	28.679606058192448	21.859033565019335
32-33	22.066410471025925	27.26804393619094	28.66474890680238	22.00079668598076
34-35	22.224458585248506	27.318399335254767	28.636054898882062	21.82108718061466
36-37	22.20470105439162	27.29962848950742	28.50569238468028	21.989978071420676
38-39	22.091963677702026	27.394329031739012	28.53679530902784	21.97691198153112
40-41	22.33204917457816	27.27487339856451	28.427548712619945	21.965528714237387
42-43	22.235221086682415	27.397447724203577	28.1841541037496	22.183177085364406
44-45	22.352454767223314	27.48068580303922	27.98672621054638	22.180133219191088
46-47	22.392841728904088	27.548674446983355	27.69587535135206	22.362608472760492
48-49	22.348109057752012	27.698486651092622	27.596153299412858	22.357250991742504
50-51	22.402177213058984	27.798732879611997	27.457493993433957	22.341595913895056
52-53	22.54173037647102	27.66135485386038	27.363509944828383	22.433404824840213
54-55	22.4903326008808	27.61523821903032	27.418243649151087	22.47618553093779
56-57	22.477142077319893	27.756080158380055	27.36271593819204	22.40406182610802
58-59	22.431147134113967	27.758108817102467	27.390101455355847	22.420642593427722
60-61	22.50613840290861	27.699088862873772	27.437105791727802	22.35766694248981
62-63	22.489690863280135	27.78343793087577	27.355050390111945	22.371820815732143
64-65	22.47904498330349	27.745640303141496	27.303551170834623	22.471763542720392
66-67	22.538269222076146	27.766469573914254	27.184555851474595	22.510705352535005
68-69	22.470074800384896	27.736838699835037	27.277375213475825	22.515711286304246
70-71	22.477080180279287	27.54490648615343	27.351508857814988	22.626504475752295
72-73	22.536548630551874	27.597371745044992	27.244612360666114	22.621467263737024
74-75	22.408485444789296	27.71775602512229	27.241549242825368	22.63220928726305
76-77	22.552760083796738	27.680641457279837	27.16218310431887	22.60441535460455
78-79	22.504164624241422	27.672138991088563	27.156172897219164	22.66752348745085
80-81	22.495777421819955	27.69730574573901	27.15629065774472	22.650626174696313
82-83	22.54309813631682	27.67052293757824	27.24656585585877	22.53981307024617
84-85	22.648665641131018	27.661421329500563	27.135683532340142	22.554229497028274
86-87	22.49262362997698	27.67083312528063	27.275887187870072	22.56065605687232
88-89	22.535802518808502	27.660440535447982	27.205903868924143	22.597853076819373
90-91	22.559158273564396	27.72992406626427	27.171353483389478	22.539564176781855
92-93	22.60710569241896	27.647061226181542	27.24084680032652	22.504986281072984
94-95	22.44873375695008	27.690171060741463	27.30667113193727	22.554424050371185
96-97	22.57852602407552	27.79824369966225	27.288674114488607	22.334556161773623
98-99	23.677507460649984	29.71477932301391	27.495901764130316	19.111811452205792
100	22.556268640377645	30.743740731494295	21.32162295620343	25.37836767192463
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1998.0
1	1820.0
2	2569.5
3	3708.5
4	5078.0
5	8533.5
6	11440.0
7	13190.0
8	14183.5
9	13824.5
10	12670.0
11	11391.0
12	12117.0
13	15837.0
14	25133.0
15	38643.0
16	50240.0
17	57433.5
18	59337.0
19	56179.5
20	49962.5
21	43072.5
22	38133.0
23	37962.5
24	42845.0
25	51998.5
26	65065.5
27	80614.0
28	98516.5
29	119961.5
30	142148.5
31	164491.0
32	187615.0
33	210217.5
34	232879.5
35	253188.0
36	274823.5
37	297637.0
38	316083.0
39	330365.5
40	343809.0
41	355697.0
42	361253.0
43	367376.0
44	375370.0
45	382661.0
46	387352.0
47	388290.5
48	387908.5
49	383368.0
50	375801.0
51	366495.5
52	355200.5
53	340147.0
54	321943.0
55	300889.5
56	277929.5
57	252808.0
58	224537.0
59	194711.5
60	163210.5
61	133606.0
62	106960.5
63	82619.0
64	61833.5
65	45866.0
66	34576.0
67	26349.0
68	19938.0
69	14097.5
70	9620.5
71	6807.5
72	4637.5
73	3078.5
74	1987.5
75	1299.5
76	860.5
77	539.5
78	347.0
79	256.0
80	200.0
81	142.5
82	109.0
83	81.0
84	54.0
85	41.5
86	34.5
87	28.0
88	22.0
89	17.0
90	11.5
91	7.5
92	6.5
93	3.5
94	1.5
95	1.5
96	1.0
97	1.5
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.1055528376377676E-5
7	0.0
8	0.0
9	0.0
10-11	0.0015791646282283256
12-13	3.1583292564566515E-4
14-15	0.007422073752673132
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	166.0
20-21	592.0
22-23	1261.0
24-25	3760.0
26-27	4152.0
28-29	6381.0
30-31	15170.0
32-33	18797.0
34-35	26662.0
36-37	38016.0
38-39	48028.0
40-41	51182.0
42-43	62093.0
44-45	74791.0
46-47	73573.0
48-49	65504.0
50-51	58652.0
52-53	56957.0
54-55	60318.0
56-57	63184.0
58-59	64699.0
60-61	63837.0
62-63	61911.0
64-65	66504.0
66-67	71185.0
68-69	73364.0
70-71	73542.0
72-73	70545.0
74-75	72663.0
76-77	72446.0
78-79	78615.0
80-81	80914.0
82-83	78772.0
84-85	79386.0
86-87	83967.0
88-89	90106.0
90-91	94971.0
92-93	100899.0
94-95	120840.0
96-97	332517.0
98-99	588986.0
100-101	6348785.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.27466263008226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.73358867731332	73.89259661059754
2	7.755624173451794	12.916939730598177
3	1.910737368095873	4.773480291086255
4	0.7173349611838229	2.38943307541384
5	0.34729344496918074	1.4460372231723784
6	0.17760249405421874	0.8873872664775753
7	0.09628012544282423	0.5612386474963231
8	0.05850102802413197	0.3897322697778062
9	0.04336584371530648	0.32501484045549184
>10	0.15417791953523208	2.0455147084785588
>50	0.004595357317377071	0.2556102580882435
>100	8.756781831584662E-4	0.10683807814415819
>500	2.2928713762578645E-5	0.010177000213632057
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.0527764188188838E-5	0.0	0.0	0.0
3	0.0	1.0527764188188838E-5	0.0	0.0	0.0
4	0.0	3.158329256456651E-5	0.0	0.0	0.0
5	0.0	3.158329256456651E-5	0.0	0.0	0.0
6	1.2633317025826605E-4	3.158329256456651E-5	0.0	0.0	0.0
7	1.2633317025826605E-4	4.211105675275535E-5	0.0	0.0	0.0
8	1.2633317025826605E-4	4.211105675275535E-5	0.0	0.0	0.0
9	1.2633317025826605E-4	7.369434931732187E-5	0.0	0.0	0.0
10-11	1.3686093444645488E-4	8.42221135055107E-5	0.0	0.0	0.0
12-13	1.4738869863464374E-4	8.42221135055107E-5	0.0	0.0	0.0
14-15	1.684442270110214E-4	1.0001375978779397E-4	0.0	0.0	0.0
16-17	2.210830479519656E-4	1.0527764188188838E-4	0.0	0.0	0.0
18-19	2.210830479519656E-4	1.2106928816417164E-4	0.0	0.0	0.0
20-21	2.210830479519656E-4	1.368609344464549E-4	0.0	5.263882094094419E-6	0.0
22-23	2.210830479519656E-4	1.5791646282283257E-4	0.0	2.1055528376377676E-5	0.0
24-25	2.210830479519656E-4	1.8423587329330468E-4	0.0	4.211105675275535E-5	0.0
26-27	2.210830479519656E-4	1.894997553873991E-4	0.0	5.790270303503861E-5	0.0
28-29	2.210830479519656E-4	1.894997553873991E-4	0.0	2.526663405165321E-4	0.0
30-31	2.2634693004606E-4	2.421385763283433E-4	0.0	8.79068309713768E-4	0.0
32-33	2.3687469423424885E-4	2.421385763283433E-4	0.0	0.002500343994694849	0.0
34-35	2.526663405165321E-4	2.7898575098700423E-4	0.0	0.004247952849934196	0.0
36-37	2.526663405165321E-4	3.1583292564566515E-4	0.0	0.006937796600016444	0.0
38-39	2.6319410470472093E-4	3.26360689833854E-4	0.0	0.011185749449950641	0.0
40-41	2.737218688929098E-4	3.579439823984205E-4	0.0	0.015923243334635618	0.0
42-43	2.8424963308109865E-4	3.737356286807038E-4	0.0	0.020587042870003274	0.0
44-45	2.8951351517519306E-4	4.369022138098368E-4	0.0	0.025556147566828405	0.0
46-47	2.947773972692875E-4	4.842771526566866E-4	0.0	0.0309358350669929	0.0
48-49	2.947773972692875E-4	4.842771526566866E-4	0.0	0.03591020364591213	0.0
50-51	3.1583292564566515E-4	5.474437377858196E-4	0.0	0.041358321613299845	0.0
52-53	3.26360689833854E-4	5.474437377858196E-4	0.0	0.04662746758948837	0.0
54-55	3.26360689833854E-4	5.474437377858196E-4	0.0	0.05145971135186704	0.0
56-57	3.26360689833854E-4	5.474437377858196E-4	0.0	0.05594980277812958	0.0
58-59	3.26360689833854E-4	5.579715019740085E-4	0.0	0.06044515808648622	0.0
60-61	3.5268010030432606E-4	5.632353840681029E-4	0.0	0.06504052715463064	0.0
62-63	4.105828033393647E-4	5.790270303503861E-4	0.0	0.06994120138423254	0.0
64-65	4.105828033393647E-4	6.000825587267638E-4	0.0	0.07445234833887146	0.0
66-67	4.105828033393647E-4	6.000825587267638E-4	0.0	0.07911614787423912	0.0
68-69	4.105828033393647E-4	6.264019691972359E-4	0.0	0.08391154446195914	0.0
70-71	4.2637444962164794E-4	6.369297333854247E-4	0.0	0.08839110812403349	0.0
72-73	4.842771526566866E-4	6.421936154795191E-4	0.0	0.09194949241964132	5.263882094094419E-6
74-75	4.842771526566866E-4	6.579852617618024E-4	0.0	0.0947919887504523	1.0527764188188838E-5
76-77	4.948049168448754E-4	6.948324364204633E-4	0.0	0.0974291936795936	1.0527764188188838E-5
78-79	5.053326810330642E-4	7.15887964796841E-4	0.0	0.09936630229022034	1.0527764188188838E-5
80-81	5.053326810330642E-4	7.15887964796841E-4	0.0	0.09992953767428844	1.0527764188188838E-5
82-83	5.053326810330642E-4	7.211518468909354E-4	0.0	0.10032959271343964	1.0527764188188838E-5
84-85	5.15860445221253E-4	7.369434931732186E-4	0.0	0.10055593964348569	1.0527764188188838E-5
86-87	5.316520915035364E-4	7.474712573614076E-4	0.0	0.1006348978748971	1.0527764188188838E-5
88	5.474437377858196E-4	8.106378424905406E-4	0.0	0.1006348978748971	1.0527764188188838E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13760	0.0	52.167316	1
GTATCAA	25270	0.0	38.119053	1
TCAACGC	30475	0.0	30.930246	4
ATCAACG	30715	0.0	30.702585	3
CAACGCA	31355	0.0	30.077011	5
TATCAAC	31800	0.0	29.93939	2
AACGCAG	31880	0.0	29.595207	6
ACGCAGA	36390	0.0	25.868155	7
CGCAGAG	36880	0.0	25.513054	8
GCAGAGT	42675	0.0	21.900635	9
GTACATG	33405	0.0	21.52714	1
TACATGG	33760	0.0	20.70129	2
GAGTACT	24040	0.0	20.324718	12-13
ACATGGG	34645	0.0	19.812035	3
GTGGTAT	6440	0.0	19.791885	1
CAGAGTA	41930	0.0	19.521517	10-11
TGGTATC	6275	0.0	19.488838	2
AGAGTAC	39330	0.0	18.720423	10-11
GTACTTT	26605	0.0	18.406012	14-15
CATGGGG	24900	0.0	18.054375	4
>>END_MODULE