FastQCFastQC Report
Fri 27 May 2016
SRR2139098_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139098_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8790885
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114350.049.8731771
GTATCAA196250.035.803521
TCAACGC231950.029.6172354
ATCAACG234150.029.3203683
CAACGCA239850.028.6059135
TATCAAC247550.028.014622
AACGCAG245500.027.9653026
ACGCAGA281100.024.3619547
CGCAGAG284850.024.0108018
GTGGTAT56300.022.5790841
GTACATG276250.022.2044771
TACATGG273250.021.6516592
ACATGGG278300.020.7112663
TGGTATC55300.020.2320482
GCAGAGT344300.019.9569
GAGTACT188450.019.87002412-13
CATGGGG211050.018.8122774
CAGAGTA331550.018.80604610-11
AGAGTAC305650.017.92802210-11
GTACTTT208350.017.89915314-15
Produced by FastQC (version 0.11.5)