##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139098_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8790885
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.557854186467004	33.0	33.0	33.0	33.0	33.0
2	32.56727917610115	33.0	33.0	33.0	33.0	33.0
3	32.56193022659266	33.0	33.0	33.0	33.0	33.0
4	36.4577580072996	37.0	37.0	37.0	37.0	37.0
5	36.48752019847831	37.0	37.0	37.0	37.0	37.0
6	36.48462344803737	37.0	37.0	37.0	37.0	37.0
7	36.46118564854392	37.0	37.0	37.0	37.0	37.0
8	36.44475795099128	37.0	37.0	37.0	37.0	37.0
9	36.481900627752495	37.0	37.0	37.0	37.0	37.0
10-11	36.4367106952258	37.0	37.0	37.0	37.0	37.0
12-13	36.36740453321821	37.0	37.0	37.0	37.0	37.0
14-15	38.394693708312644	40.0	37.0	40.0	37.0	40.0
16-17	38.35358265976634	40.0	37.0	40.0	37.0	40.0
18-19	38.35783206127711	40.0	37.0	40.0	37.0	40.0
20-21	38.35157034592788	40.0	37.0	40.0	37.0	40.0
22-23	38.34643799470719	40.0	37.0	40.0	37.0	40.0
24-25	38.345624932828485	40.0	37.0	40.0	37.0	40.0
26-27	38.19601119603966	40.0	37.0	40.0	37.0	40.0
28-29	38.12013244172657	40.0	37.0	40.0	33.0	40.0
30-31	38.08868259792558	40.0	37.0	40.0	33.0	40.0
32-33	38.0131122254859	40.0	37.0	40.0	33.0	40.0
34-35	37.97559661056776	40.0	37.0	40.0	33.0	40.0
36-37	37.93753366934811	40.0	37.0	40.0	33.0	40.0
38-39	37.89199051464996	40.0	37.0	40.0	33.0	40.0
40-41	37.85854048353855	40.0	37.0	40.0	33.0	40.0
42-43	37.76050269345357	40.0	37.0	40.0	33.0	40.0
44-45	37.71248723378454	40.0	37.0	40.0	33.0	40.0
46-47	37.67505110698097	40.0	37.0	40.0	33.0	40.0
48-49	37.645170371328504	40.0	37.0	40.0	33.0	40.0
50-51	37.580513551675736	40.0	37.0	40.0	33.0	40.0
52-53	37.47503163183448	40.0	37.0	40.0	33.0	40.0
54-55	37.26208560662553	40.0	37.0	40.0	33.0	40.0
56-57	37.07338669272071	38.5	37.0	40.0	33.0	40.0
58-59	37.009596407094264	37.0	37.0	40.0	33.0	40.0
60-61	36.87138297440336	37.0	37.0	40.0	33.0	40.0
62-63	36.68215871993921	37.0	37.0	40.0	33.0	40.0
64-65	36.52985204294012	37.0	37.0	40.0	33.0	40.0
66-67	36.30664534211812	37.0	37.0	40.0	33.0	40.0
68-69	36.08195120773641	37.0	37.0	40.0	33.0	40.0
70-71	35.81858156095231	37.0	35.0	37.0	33.0	40.0
72-73	35.60845737749085	37.0	33.0	37.0	33.0	40.0
74-75	35.430834529216995	37.0	33.0	37.0	33.0	40.0
76-77	33.64336838059549	35.0	33.0	37.0	27.0	37.0
78-79	34.88956966087851	37.0	33.0	37.0	33.0	37.0
80-81	34.96398158454723	37.0	33.0	37.0	33.0	37.0
82-83	34.85552435907917	37.0	33.0	37.0	33.0	37.0
84-85	34.70505386115928	37.0	33.0	37.0	33.0	37.0
86-87	34.58470890794153	37.0	33.0	37.0	33.0	37.0
88-89	34.509983048868605	37.0	33.0	37.0	33.0	37.0
90-91	34.42647710397418	37.0	33.0	37.0	30.0	37.0
92-93	34.35972950297789	37.0	33.0	37.0	30.0	37.0
94-95	34.35399644708209	37.0	33.0	37.0	30.0	37.0
96-97	34.36233957586235	37.0	33.0	37.0	33.0	37.0
98-99	34.367049902686354	37.0	33.0	37.0	33.0	37.0
100	34.37456507740409	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	10.0
20	100.0
21	391.0
22	1607.0
23	4403.0
24	9910.0
25	19687.0
26	34078.0
27	53916.0
28	78740.0
29	109115.0
30	146347.0
31	192013.0
32	252894.0
33	333868.0
34	454690.0
35	653582.0
36	1116007.0
37	2522333.0
38	2752654.0
39	54539.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.75139363101667	19.978841720714126	14.279745440874269	23.990019207394933
2	14.73326064440611	20.664654355050715	41.855842728007474	22.7462422725357
3	18.576047804060682	25.7175699602486	29.869245246638993	25.83713698905173
4	12.688096818465947	17.787208000104656	38.439588278085765	31.085106903343636
5	13.747717095605278	37.52817833471829	35.17714086806959	13.546963701606835
6	30.56310377886911	37.39195057061383	18.569770685177964	13.475174965339095
7	27.0846905630093	32.23769847973213	22.738233977580187	17.939376979678382
8	24.459346243296324	37.115660141157576	20.699292505817105	17.725701109729
9	25.665459165942906	16.928898512493337	20.885985881967514	36.51965643959624
10-11	24.70054150715938	26.634593152967454	28.405302509998442	20.25956282987472
12-13	25.44362833174134	24.00792709610583	28.608301280159022	21.94014329199381
14-15	22.430232785664817	25.425483650488406	26.218272737283836	25.92601082656294
16-17	22.14802036427504	28.515177937147396	26.904048909751406	22.432752788826154
18-19	22.176055084328823	27.718460655554022	28.552807823103134	21.55267643701402
20-21	22.961146303464368	26.902609606006106	28.423690540063763	21.71255355046577
22-23	22.690943444029497	26.832113546412415	28.27305919465337	22.203883814904714
24-25	22.460276700069507	26.940345682501825	28.358642196245714	22.240735421182958
26-27	22.3801800410296	27.079399224310986	28.30092531439342	22.239495420265996
28-29	22.304835274684713	27.17639340708178	28.30538194605557	22.213389372177932
30-31	22.58908583213677	27.006021947394693	28.1951701008839	22.209722119584637
32-33	22.380985585817125	27.161807881956673	28.159520593923403	22.2976859383028
34-35	22.503124431856513	27.212332158264907	28.115661722079356	22.16888168779922
36-37	22.49019264413194	27.166053262004674	28.07502924648834	22.26872484737504
38-39	22.344965565428506	27.288935236963578	28.129296622235028	22.236802575372884
40-41	22.604784860998773	27.1048550119407	28.013254768904982	22.277105358155545
42-43	22.485449804258565	27.233775808851973	27.83928830384255	22.44148608304691
44-45	22.570813667539987	27.310836338205156	27.69342501006497	22.424924984189886
46-47	22.694290876832543	27.33539608077985	27.42279344761178	22.547519594775824
48-49	22.542428387275468	27.499283849041067	27.37604422872439	22.582243534959076
50-51	22.582062355921153	27.581105980421473	27.31039236922691	22.52643929443047
52-53	22.750999677303884	27.44909770124145	27.214892644621504	22.585009976833163
54-55	22.695520434668616	27.37016540070832	27.265350272596518	22.66896389202655
56-57	22.68605439533819	27.50399098637918	27.218141913694726	22.59181270458791
58-59	22.686064856759515	27.51540432119997	27.225144408167466	22.57338641387305
60-61	22.711009642606488	27.473357248920426	27.286822057786353	22.528811050686738
62-63	22.688725882389953	27.580076618122572	27.186775694491182	22.54442180499629
64-65	22.668003182464755	27.52012391563558	27.168937163982132	22.642935737917536
66-67	22.729912435119562	27.51462138046567	27.082675706677623	22.67279047773715
68-69	22.67086416561309	27.48006923666264	27.15777194149565	22.69129465622862
70-71	22.650950836243677	27.32826266410503	27.233465552920254	22.787320946731036
72-73	22.720007976825734	27.371353685374768	27.108938902340395	22.799699435459104
74-75	22.55414631894767	27.560598989916375	27.110049837608262	22.77520485352769
76-77	22.748840159367433	27.424494592389586	27.074921996439134	22.751743251803845
78-79	22.683444581676042	27.441379304928663	27.02575561874375	22.849420494651547
80-81	22.648842254613246	27.529253384147097	27.050171991388055	22.7717323698516
82-83	22.709784744395574	27.455094820142666	27.105028998103975	22.73009143735778
84-85	22.764902356976485	27.465800056208145	27.076021346744174	22.693276240071196
86-87	22.649966427801626	27.46845039453766	27.155547754996352	22.726035422664363
88-89	22.66449486370788	27.43597358341609	27.122399363718063	22.777132189157967
90-91	22.686682959265625	27.50888695066451	27.09948099724523	22.704949092824638
92-93	22.727134895512155	27.470921722395985	27.09322585860517	22.70871752348669
94-95	22.61660426969361	27.509529365730227	27.151351163937637	22.722515200638522
96-97	22.70448514372672	27.606985374133124	27.199775406038107	22.488754076102047
98-99	23.81348026650593	29.562804873374642	27.35983979232199	19.26387506779744
100	22.73414016147055	30.53261542416183	21.19855027987179	25.53469413449584
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1303.0
1	1203.5
2	1720.5
3	2491.5
4	3408.5
5	5731.5
6	7760.5
7	8996.0
8	9596.0
9	9396.0
10	8767.0
11	7997.0
12	8554.0
13	11192.0
14	17835.0
15	27473.0
16	35657.5
17	40632.0
18	41997.0
19	39953.0
20	35811.0
21	31066.0
22	27840.0
23	28111.0
24	32432.0
25	40421.0
26	51196.0
27	64312.5
28	80013.5
29	98563.5
30	117863.0
31	138057.5
32	159782.0
33	180569.0
34	201902.5
35	221540.5
36	241808.0
37	263673.5
38	282075.0
39	296002.5
40	310670.0
41	325534.5
42	335461.0
43	344074.5
44	352144.0
45	361072.0
46	367412.5
47	369520.5
48	369977.0
49	367045.5
50	361692.5
51	353946.5
52	341106.0
53	323798.0
54	306625.0
55	287842.0
56	265512.5
57	240592.5
58	214322.5
59	185832.0
60	154547.0
61	125932.0
62	100441.5
63	77077.0
64	56998.5
65	41600.5
66	31052.5
67	23614.0
68	17627.0
69	12427.5
70	8457.0
71	5786.0
72	3828.0
73	2474.0
74	1655.0
75	1091.5
76	739.5
77	500.5
78	358.0
79	265.5
80	178.0
81	113.0
82	76.5
83	55.0
84	43.5
85	33.0
86	25.5
87	19.0
88	13.0
89	11.0
90	10.5
91	8.5
92	7.5
93	4.5
94	2.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.1375418970899971E-5
7	0.0
8	0.0
9	0.0
10-11	0.001620997203353246
12-13	2.7301005530159936E-4
14-15	0.007428148587997681
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	135.0
20-21	538.0
22-23	1099.0
24-25	2740.0
26-27	3085.0
28-29	4953.0
30-31	12469.0
32-33	15551.0
34-35	21676.0
36-37	32760.0
38-39	40181.0
40-41	41623.0
42-43	49053.0
44-45	59179.0
46-47	58311.0
48-49	52532.0
50-51	46468.0
52-53	45109.0
54-55	48175.0
56-57	50998.0
58-59	51308.0
60-61	50821.0
62-63	49109.0
64-65	52490.0
66-67	56687.0
68-69	58580.0
70-71	58541.0
72-73	56543.0
74-75	57733.0
76-77	57044.0
78-79	62528.0
80-81	64665.0
82-83	63152.0
84-85	64169.0
86-87	68964.0
88-89	74667.0
90-91	79804.0
92-93	86515.0
94-95	108052.0
96-97	313913.0
98-99	569029.0
100-101	6099936.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.69761258073524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.35401723487021	73.06666287240901
2	7.928291509462307	13.113015593532928
3	2.00336306865515	4.970200287305894
4	0.7824123786426842	2.588145430694569
5	0.34828179777773544	1.4401036590772571
6	0.18800472012084718	0.9328524904742027
7	0.1075107765008863	0.6223619180327016
8	0.07075788233523145	0.4681206352313776
9	0.049533306850030386	0.3686657597753843
>10	0.16331617753444647	2.1052589849214773
>50	0.0036545000059040895	0.20806041747011292
>100	8.317296452829221E-4	0.09662569791946181
>500	2.4917599291714675E-5	0.019926253155701717
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1375418970899971E-5	0.0	0.0	0.0
3	0.0	1.1375418970899971E-5	0.0	0.0	0.0
4	0.0	5.687709485449986E-5	0.0	0.0	1.1375418970899971E-5
5	0.0	5.687709485449986E-5	0.0	0.0	1.1375418970899971E-5
6	6.825251382539983E-5	5.687709485449986E-5	0.0	0.0	2.2750837941799942E-5
7	6.825251382539983E-5	5.687709485449986E-5	0.0	1.1375418970899971E-5	2.2750837941799942E-5
8	6.825251382539983E-5	9.100335176719977E-5	0.0	1.1375418970899971E-5	3.4126256912699914E-5
9	6.825251382539983E-5	1.592558655925996E-4	0.0	1.1375418970899971E-5	5.687709485449986E-5
10-11	6.825251382539983E-5	1.592558655925996E-4	0.0	1.1375418970899971E-5	5.687709485449986E-5
12-13	6.825251382539983E-5	1.592558655925996E-4	0.0	1.1375418970899971E-5	5.687709485449986E-5
14-15	7.39402233108498E-5	2.047575414761995E-4	0.0	1.1375418970899971E-5	7.39402233108498E-5
16-17	1.3650502765079965E-4	2.047575414761995E-4	0.0	1.1375418970899971E-5	9.100335176719977E-5
18-19	1.4788044662169964E-4	2.3319608890344943E-4	0.0	1.1375418970899971E-5	1.0806648022354973E-4
20-21	1.4788044662169964E-4	2.502592173597994E-4	0.0	2.2750837941799942E-5	1.251296086798997E-4
22-23	1.4788044662169964E-4	3.0144860272884926E-4	0.0	2.2750837941799942E-5	1.5356815610714962E-4
24-25	1.5925586559259962E-4	3.583256975833491E-4	0.0	2.2750837941799942E-5	1.7631899404894956E-4
26-27	1.7063128456349958E-4	3.98139663981499E-4	0.0	7.39402233108498E-5	1.8200670353439954E-4
28-29	1.7063128456349958E-4	3.98139663981499E-4	0.0	1.5356815610714962E-4	1.9338212250529952E-4
30-31	1.7063128456349958E-4	4.6639217780689886E-4	0.0	5.118938536904987E-4	2.047575414761995E-4
32-33	1.7631899404894956E-4	4.777675967777988E-4	0.0	0.0013764256954788966	2.047575414761995E-4
34-35	2.047575414761995E-4	5.915217864867986E-4	0.0	0.0024627782071998438	2.047575414761995E-4
36-37	2.047575414761995E-4	6.768374287685483E-4	0.0	0.00429990837100019	2.1613296044709947E-4
38-39	2.047575414761995E-4	6.995882667103483E-4	0.0	0.007189264789608782	2.2750837941799945E-4
40-41	2.047575414761995E-4	7.507776520793982E-4	0.0	0.010357318973004424	2.2750837941799945E-4
42-43	2.047575414761995E-4	8.01967037448448E-4	0.0	0.013576562541769116	2.2750837941799945E-4
44-45	2.047575414761995E-4	9.157212271574478E-4	0.0	0.01694368655715551	2.2750837941799945E-4
46-47	2.047575414761995E-4	9.839737409828475E-4	0.0	0.0202880597346001	2.2750837941799945E-4
48-49	2.104452509616495E-4	9.953491599537477E-4	0.0	0.02403626028551164	2.388837983888994E-4
50-51	2.2750837941799942E-4	0.0010408508358373475	0.0	0.02788115189767583	2.502592173597994E-4
52-53	2.388837983888994E-4	0.0010579139642936974	0.0	0.03144734574505297	2.786977647870493E-4
54-55	2.388837983888994E-4	0.0010579139642936974	0.0	0.03497941333551741	3.128240216997492E-4
56-57	2.6163463633069935E-4	0.0010579139642936974	0.0	0.038670736791574456	3.185117311851992E-4
58-59	2.6163463633069935E-4	0.0010692893832645973	0.0	0.0427715753305839	3.185117311851992E-4
60-61	2.900731837579493E-4	0.0010806648022354973	0.0	0.047060108282613186	3.355748596415492E-4
62-63	3.4126256912699916E-4	0.0011034156401772972	0.0	0.05109838201728267	3.526379880978991E-4
64-65	3.4126256912699916E-4	0.001194418991944497	0.0	0.05525609765114661	3.526379880978991E-4
66-67	3.4695027861244914E-4	0.001194418991944497	0.0	0.058924670269261854	3.583256975833491E-4
68-69	3.526379880978991E-4	0.0012456083773135468	0.0	0.06308807361261125	3.6970111655424906E-4
70-71	3.640134070687991E-4	0.0012854223437116967	0.0	0.06664289204101748	3.753888260396991E-4
72-73	3.98139663981499E-4	0.0013309240195952968	0.0	0.06962325181139328	4.09515082952399E-4
74-75	3.98139663981499E-4	0.0013821134049643467	0.0	0.07193246186248597	4.09515082952399E-4
76-77	3.98139663981499E-4	0.0014389904998188463	0.0	0.07427579817049138	4.09515082952399E-4
78-79	4.09515082952399E-4	0.0014560536282751963	0.0	0.07579441660310651	4.09515082952399E-4
80-81	4.09515082952399E-4	0.0014560536282751963	0.0	0.0764086892275351	4.379536303796489E-4
82-83	4.15202792437849E-4	0.0014560536282751963	0.0	0.07677270263460391	5.062061442050488E-4
84-85	4.20890501923299E-4	0.0014560536282751963	0.0	0.0769092076622547	5.460201106031986E-4
86-87	4.2657821140874895E-4	0.0014788044662169964	0.0	0.0769774601760801	5.460201106031986E-4
88	4.436413398650989E-4	0.0015243061421005963	0.0	0.0769774601760801	5.460201106031986E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11435	0.0	49.873177	1
GTATCAA	19625	0.0	35.80352	1
TCAACGC	23195	0.0	29.617235	4
ATCAACG	23415	0.0	29.320368	3
CAACGCA	23985	0.0	28.605913	5
TATCAAC	24755	0.0	28.01462	2
AACGCAG	24550	0.0	27.965302	6
ACGCAGA	28110	0.0	24.361954	7
CGCAGAG	28485	0.0	24.010801	8
GTGGTAT	5630	0.0	22.579084	1
GTACATG	27625	0.0	22.204477	1
TACATGG	27325	0.0	21.651659	2
ACATGGG	27830	0.0	20.711266	3
TGGTATC	5530	0.0	20.232048	2
GCAGAGT	34430	0.0	19.956	9
GAGTACT	18845	0.0	19.870024	12-13
CATGGGG	21105	0.0	18.812277	4
CAGAGTA	33155	0.0	18.806046	10-11
AGAGTAC	30565	0.0	17.928022	10-11
GTACTTT	20835	0.0	17.899153	14-15
>>END_MODULE