FastQCFastQC Report
Fri 27 May 2016
SRR2139097_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139097_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9519825
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114700.048.5856441
GTATCAA208100.038.1465031
TCAACGC251200.030.91414
ATCAACG255000.030.4534233
CAACGCA260200.029.8122675
TATCAAC266400.029.3608822
AACGCAG267250.029.0742846
ACGCAGA308050.025.2375247
CGCAGAG311850.024.8888638
GTACATG295650.022.7767541
TACATGG299850.021.997052
ACATGGG304200.021.043943
GCAGAGT374400.020.6413279
GAGTACT207700.019.20643412-13
CATGGGG235800.018.7273774
AGAGTAC334200.018.56396510-11
CAGAGTA363800.018.13347610-11
GTGGTAT61600.018.0093291
TGGTATC58550.017.178062
GTACTTT234100.016.92109714-15
Produced by FastQC (version 0.11.5)