##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139097_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9519825
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55932572289932	33.0	33.0	33.0	33.0	33.0
2	32.56368420637985	33.0	33.0	33.0	33.0	33.0
3	32.5569424858125	33.0	33.0	33.0	33.0	33.0
4	36.45295097336348	37.0	37.0	37.0	37.0	37.0
5	36.483171486870816	37.0	37.0	37.0	37.0	37.0
6	36.485192532425756	37.0	37.0	37.0	37.0	37.0
7	36.463206308939505	37.0	37.0	37.0	37.0	37.0
8	36.44521826819295	37.0	37.0	37.0	37.0	37.0
9	36.48098688788922	37.0	37.0	37.0	37.0	37.0
10-11	36.43477364342307	37.0	37.0	37.0	37.0	37.0
12-13	36.366425328196684	37.0	37.0	37.0	37.0	37.0
14-15	38.38668841076385	40.0	37.0	40.0	37.0	40.0
16-17	38.34922453931664	40.0	37.0	40.0	37.0	40.0
18-19	38.35392551858884	40.0	37.0	40.0	37.0	40.0
20-21	38.34886219069092	40.0	37.0	40.0	37.0	40.0
22-23	38.346473917342166	40.0	37.0	40.0	37.0	40.0
24-25	38.34562153411645	40.0	37.0	40.0	37.0	40.0
26-27	38.19626096837402	40.0	37.0	40.0	37.0	40.0
28-29	38.120185608394436	40.0	37.0	40.0	33.0	40.0
30-31	38.08450015760192	40.0	37.0	40.0	33.0	40.0
32-33	38.00878032941546	40.0	37.0	40.0	33.0	40.0
34-35	37.970386143035824	40.0	37.0	40.0	33.0	40.0
36-37	37.93045603996214	40.0	37.0	40.0	33.0	40.0
38-39	37.88486231406594	40.0	37.0	40.0	33.0	40.0
40-41	37.8531032696296	40.0	37.0	40.0	33.0	40.0
42-43	37.75797292924531	40.0	37.0	40.0	33.0	40.0
44-45	37.7095538006525	40.0	37.0	40.0	33.0	40.0
46-47	37.675011177103656	40.0	37.0	40.0	33.0	40.0
48-49	37.64884524864439	40.0	37.0	40.0	33.0	40.0
50-51	37.58216200110783	40.0	37.0	40.0	33.0	40.0
52-53	37.48021080308901	40.0	37.0	40.0	33.0	40.0
54-55	37.27034812011857	40.0	37.0	40.0	33.0	40.0
56-57	37.08332122471862	38.5	37.0	40.0	33.0	40.0
58-59	37.01960238796932	37.0	37.0	40.0	33.0	40.0
60-61	36.88049904136017	37.0	37.0	40.0	33.0	40.0
62-63	36.6969909002006	37.0	37.0	40.0	33.0	40.0
64-65	36.545714253806324	37.0	37.0	40.0	33.0	40.0
66-67	36.32547682179183	37.0	37.0	40.0	33.0	40.0
68-69	36.105540716072525	37.0	37.0	40.0	33.0	40.0
70-71	35.84563765767642	37.0	35.0	37.0	33.0	40.0
72-73	35.64174817642103	37.0	33.0	37.0	33.0	40.0
74-75	35.46566090788458	37.0	33.0	37.0	33.0	40.0
76-77	33.66628473682577	35.0	33.0	37.0	27.0	37.0
78-79	34.91739942897077	37.0	33.0	37.0	33.0	37.0
80-81	34.994451944201145	37.0	33.0	37.0	33.0	37.0
82-83	34.88983121315786	37.0	33.0	37.0	33.0	37.0
84-85	34.74421609053056	37.0	33.0	37.0	33.0	37.0
86-87	34.626241988069694	37.0	33.0	37.0	33.0	37.0
88-89	34.55361835225855	37.0	33.0	37.0	33.0	37.0
90-91	34.47787348990396	37.0	33.0	37.0	33.0	37.0
92-93	34.41112781107827	37.0	33.0	37.0	30.0	37.0
94-95	34.40210165045019	37.0	33.0	37.0	33.0	37.0
96-97	34.41126517228152	37.0	33.0	37.0	33.0	37.0
98-99	34.416322535194084	37.0	33.0	37.0	33.0	37.0
100	34.426015921318225	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	18.0
20	88.0
21	458.0
22	1695.0
23	4772.0
24	10663.0
25	21338.0
26	36312.0
27	57007.0
28	83284.0
29	115711.0
30	155950.0
31	205511.0
32	270327.0
33	359271.0
34	488627.0
35	700423.0
36	1193842.0
37	2724109.0
38	3020554.0
39	69862.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.50670836911392	20.17719863547912	14.314643388927841	24.00144960647911
2	14.694555834797383	20.87131853789329	41.74260556260225	22.69152006470707
3	18.329948292116715	26.083431155509686	30.143936469420396	25.442684082953203
4	12.64534799746844	17.981832649234626	38.46703064394566	30.905788709351274
5	13.658917049420552	37.63311825585029	35.21911379673471	13.488850897994448
6	30.260106781419527	37.49593611056342	18.72434366150372	13.51961344651333
7	26.727371564078123	32.44212997612876	22.975758482955307	17.85473997683781
8	24.452319239061644	36.869721869887314	20.964944208533247	17.713014682517798
9	25.74899223462616	16.85294635142978	21.01491361448346	36.383147799460595
10-11	24.553981669124035	26.61919376247679	28.560845978371535	20.265978590027643
12-13	25.393158103857722	24.099706900432018	28.684796769680883	21.822338226029377
14-15	22.42981656089015	25.422901220133028	26.320115943417495	25.827166275559332
16-17	22.08539022513544	28.518013723991775	26.966041917787354	22.43055413308543
18-19	22.15383160929954	27.660319386123167	28.64025861819939	21.5455903863779
20-21	22.908538228964737	26.866903120122544	28.501279785771654	21.72327886514106
22-23	22.67597783586228	26.834020204350495	28.341380752305113	22.148621207482112
24-25	22.438493816086176	26.98984136033863	28.376696549983887	22.194968273591304
26-27	22.371805032401124	27.115007271490416	28.341217263052208	22.171970433056252
28-29	22.31352745995736	27.142729904861735	28.360434325320007	22.1833083098609
30-31	22.560564048041325	27.07097511557402	28.197008480558488	22.171452355826165
32-33	22.421219589658495	27.13621722689709	28.197446531125784	22.24511665231863
34-35	22.524483495887676	27.165906619749165	28.176126111947546	22.13348377241561
36-37	22.49567309923047	27.17483306288217	28.085208959274695	22.244284878612664
38-39	22.354758360100654	27.22111323334881	28.147515200062124	22.27661320648841
40-41	22.627289392902906	27.058860188579096	28.01925460198232	22.294595816535683
42-43	22.519964724021246	27.18150550788161	27.832878357899	22.465651410198145
44-45	22.55878556756277	27.28225819932263	27.67632316248223	22.482633070632374
46-47	22.666171274122586	27.28809431297209	27.413605023001303	22.632129389904023
48-49	22.584043263741236	27.444102813383136	27.337509602367398	22.63434432050823
50-51	22.609812200803464	27.548013375635378	27.279874904829526	22.56229951873163
52-53	22.810830760975414	27.41801598081239	27.169839360393755	22.60131389781844
54-55	22.694957183016893	27.34246125745997	27.215365610709753	22.747215948813388
56-57	22.693199444259125	27.45817079468206	27.188686177109524	22.659943583949293
58-59	22.694960945145194	27.504627277140298	27.181109538412073	22.61930223930243
60-61	22.739275401344887	27.51078762978423	27.164177892516957	22.585759076353927
62-63	22.72945346777909	27.587652073263623	27.13324262695772	22.549651831999565
64-65	22.722842864909964	27.52651628237171	27.0599276803337	22.690713172384623
66-67	22.739213118410444	27.48564638499067	27.004361682838308	22.770778813760582
68-69	22.717541332543377	27.476751274187734	27.061351615623426	22.744355777645463
70-71	22.710803177352584	27.341654687606955	27.112162689023613	22.83537944601685
72-73	22.77610473022201	27.336963318521562	27.028868270634927	22.858063680621502
74-75	22.608915068112907	27.49465641625467	27.041216291581343	22.855212224051087
76-77	22.796140759800284	27.394252854743428	27.000202099241726	22.80940428621456
78-79	22.730019352145483	27.42703765194215	26.944753752287237	22.898189243625133
80-81	22.736895990388916	27.47903708368194	26.971355900566437	22.812711025362702
82-83	22.77574735546692	27.41886439363766	27.02724476440655	22.778143486488872
84-85	22.823430972336794	27.425893484099813	26.99140358190228	22.759271961661113
86-87	22.7144142119148	27.420020495436027	27.05416168647426	22.81140360617492
88-89	22.741042000161602	27.387523035691164	27.02224244239495	22.84919252175228
90-91	22.752599724960085	27.45710260854371	26.98689405576801	22.8034036107282
92-93	22.7931237120234	27.389465226395117	27.059238872534262	22.75817218904722
94-95	22.69370472629356	27.425464130983713	27.07725623895529	22.803574903767437
96-97	22.80383973938398	27.508635446855667	27.094639483996367	22.592885329763984
98-99	23.90271087457955	29.43269454103564	27.289772478633058	19.37482210575175
100	22.797208367496477	30.42181034630098	21.03549564271946	25.745485643483086
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1474.0
1	1394.0
2	2121.0
3	3072.0
4	4179.5
5	7123.5
6	9655.5
7	11025.5
8	11629.0
9	11159.5
10	10147.0
11	9153.0
12	9818.0
13	12834.5
14	20459.0
15	31921.5
16	41777.0
17	47770.5
18	49473.5
19	47126.5
20	42497.0
21	37136.5
22	33267.0
23	33035.5
24	37344.5
25	45955.0
26	57990.5
27	72590.0
28	89840.5
29	110649.5
30	132723.5
31	155742.5
32	179717.5
33	202639.0
34	224995.5
35	246081.5
36	268866.0
37	291401.0
38	310591.0
39	325604.0
40	338650.5
41	351995.0
42	361508.5
43	370004.0
44	377497.5
45	385188.5
46	392036.5
47	395178.5
48	396963.5
49	393631.0
50	387387.5
51	380709.5
52	369828.0
53	353278.0
54	334818.5
55	314023.0
56	290263.0
57	263946.0
58	235933.0
59	205393.0
60	170952.5
61	140017.5
62	112777.5
63	86957.0
64	64435.0
65	47486.5
66	35922.0
67	27397.0
68	20373.5
69	14470.5
70	10121.5
71	7065.0
72	4626.0
73	2946.5
74	1907.0
75	1217.0
76	788.0
77	519.0
78	371.5
79	263.5
80	188.0
81	132.5
82	89.0
83	54.5
84	34.0
85	26.0
86	24.0
87	21.0
88	15.5
89	9.5
90	5.5
91	3.5
92	2.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	4.201757910465791E-5
7	0.0
8	0.0
9	0.0
10-11	0.0015651548216485073
12-13	2.3634888246370074E-4
14-15	0.0073425719485389705
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	168.0
20-21	536.0
22-23	1154.0
24-25	3170.0
26-27	3794.0
28-29	5841.0
30-31	14265.0
32-33	18015.0
34-35	24817.0
36-37	36455.0
38-39	45153.0
40-41	48481.0
42-43	58384.0
44-45	69729.0
46-47	68618.0
48-49	62184.0
50-51	55702.0
52-53	55452.0
54-55	59952.0
56-57	63487.0
58-59	64424.0
60-61	65006.0
62-63	63649.0
64-65	67236.0
66-67	73079.0
68-69	72710.0
70-71	72703.0
72-73	70586.0
74-75	73273.0
76-77	72450.0
78-79	78454.0
80-81	80783.0
82-83	79188.0
84-85	80032.0
86-87	84428.0
88-89	90882.0
90-91	96011.0
92-93	101843.0
94-95	122854.0
96-97	339335.0
98-99	598055.0
100-101	6377487.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.88442169014449
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.58594226385028	73.4239459441576
2	7.734334342191087	12.821116582214644
3	1.9704890299224374	4.899685310756851
4	0.7611766281511576	2.523587385134514
5	0.35399491306048697	1.467033182513572
6	0.18685578138281408	0.9292460027624768
7	0.12064306431851118	0.6999601431876693
8	0.07094275199442454	0.47040391777321783
9	0.04975407864501791	0.3711454231696449
>10	0.16165035740796535	2.109787623554148
>50	0.0035141588662545684	0.19729206881586342
>100	7.026302095946174E-4	0.08679641595986201
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.1008789552328956E-5	0.0	0.0	0.0
3	0.0	2.1008789552328956E-5	0.0	0.0	0.0
4	0.0	5.252197388082239E-5	0.0	1.0504394776164478E-5	0.0
5	0.0	6.302636865698687E-5	0.0	1.0504394776164478E-5	0.0
6	0.0	6.302636865698687E-5	0.0	1.0504394776164478E-5	2.1008789552328956E-5
7	0.0	6.302636865698687E-5	0.0	1.0504394776164478E-5	2.1008789552328956E-5
8	0.0	7.353076343315134E-5	0.0	1.0504394776164478E-5	3.1513184328493436E-5
9	0.0	1.5756592164246718E-4	0.0	4.201757910465791E-5	3.1513184328493436E-5
10-11	0.0	1.5756592164246718E-4	0.0	5.252197388082239E-5	4.201757910465791E-5
12-13	0.0	1.5756592164246718E-4	0.0	5.777417126890463E-5	4.201757910465791E-5
14-15	0.0	1.9958350074712508E-4	0.0	6.302636865698687E-5	5.252197388082239E-5
16-17	0.0	2.1008789552328955E-4	0.0	6.302636865698687E-5	6.302636865698687E-5
18-19	0.0	2.3109668507561852E-4	0.0	7.353076343315134E-5	7.353076343315134E-5
20-21	0.0	2.62609869404112E-4	0.0	8.928735559739806E-5	7.878296082123359E-5
22-23	0.0	2.8361865895644087E-4	0.0	9.979175037356254E-5	9.453955298548031E-5
24-25	0.0	3.1513184328493436E-4	0.0	1.2605273731397374E-4	1.0504394776164478E-4
26-27	0.0	3.256362380610988E-4	0.0	2.3634888246370074E-4	1.0504394776164478E-4
28-29	0.0	3.256362380610988E-4	0.0	5.93498304853293E-4	1.0504394776164478E-4
30-31	0.0	3.466450276134278E-4	0.0	0.0023634888246370075	1.0504394776164478E-4
32-33	0.0	3.466450276134278E-4	0.0	0.006864621986223486	1.0504394776164478E-4
34-35	0.0	3.939148041061679E-4	0.0	0.012379429243709838	1.0504394776164478E-4
36-37	0.0	4.4118458059890804E-4	0.0	0.019890071508667437	1.1554834253780926E-4
38-39	0.0	4.6219337015123704E-4	0.0	0.030462744850876987	1.2605273731397374E-4
40-41	0.0	5.252197388082239E-4	0.0	0.04061524240203995	1.365571320901382E-4
42-43	0.0	5.462285283605528E-4	0.0	0.05018999824051387	1.4180932947822043E-4
44-45	0.0	5.987505022413753E-4	0.0	0.060111399106601224	1.4706152686630268E-4
46-47	0.0	6.407680813460331E-4	0.0	0.06886681215253432	1.6281811903054943E-4
48-49	0.0	6.407680813460331E-4	0.0	0.07652976814174631	1.7857471119479612E-4
50-51	0.0	7.405598317195958E-4	0.0	0.08311077146901336	1.7857471119479612E-4
52-53	0.0	7.45812029107678E-4	0.0	0.08955521766419025	1.9433130335904284E-4
54-55	0.0	7.563164238838425E-4	0.0	0.09505952052690045	2.1008789552328955E-4
56-57	0.0	7.668208186600068E-4	0.0	0.09970246301796515	2.1008789552328955E-4
58-59	0.0	7.720730160480891E-4	0.0	0.10445570165417957	2.153400929113718E-4
60-61	0.0	7.983340029885003E-4	0.0	0.10924570567211056	2.2059229029945402E-4
62-63	0.0	8.035862003765825E-4	0.0	0.11417751901951978	2.3109668507561852E-4
64-65	0.0	0.0010189262932879543	0.0	0.11858936482550887	2.3109668507561852E-4
66-67	0.0	0.0010189262932879543	0.0	0.12341088202776837	2.3109668507561852E-4
68-69	0.0	0.0010451872802283656	0.0	0.1284529915203273	2.3634888246370074E-4
70-71	0.0	0.0010504394776164477	0.0	0.13311694280094433	2.4685327723986524E-4
72-73	0.0	0.00105569167500453	0.0	0.1366149062614071	2.9412305373260536E-4
74-75	0.0	0.0010819526619449413	0.0	0.1397189549177637	3.0462744850876986E-4
76-77	0.0	0.0011502312279900104	0.0	0.14270745523158251	3.0462744850876986E-4
78-79	0.0	0.001186996609706586	0.0	0.1446034984886802	3.0462744850876986E-4
80-81	0.0	0.001186996609706586	0.0	0.1453177973334594	3.098796458968521E-4
82-83	0.0	0.0011975010044827507	0.0	0.14576948630883446	3.1513184328493436E-4
84-85	0.0	0.0012185097940350794	0.0	0.14596906980958158	3.1513184328493436E-4
86-87	0.0	0.0012395185835874084	0.0	0.14603734837562665	3.1513184328493436E-4
88	0.0	0.0012710317679159018	0.0	0.14604260057301474	3.1513184328493436E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11470	0.0	48.585644	1
GTATCAA	20810	0.0	38.146503	1
TCAACGC	25120	0.0	30.9141	4
ATCAACG	25500	0.0	30.453423	3
CAACGCA	26020	0.0	29.812267	5
TATCAAC	26640	0.0	29.360882	2
AACGCAG	26725	0.0	29.074284	6
ACGCAGA	30805	0.0	25.237524	7
CGCAGAG	31185	0.0	24.888863	8
GTACATG	29565	0.0	22.776754	1
TACATGG	29985	0.0	21.99705	2
ACATGGG	30420	0.0	21.04394	3
GCAGAGT	37440	0.0	20.641327	9
GAGTACT	20770	0.0	19.206434	12-13
CATGGGG	23580	0.0	18.727377	4
AGAGTAC	33420	0.0	18.563965	10-11
CAGAGTA	36380	0.0	18.133476	10-11
GTGGTAT	6160	0.0	18.009329	1
TGGTATC	5855	0.0	17.17806	2
GTACTTT	23410	0.0	16.921097	14-15
>>END_MODULE