FastQCFastQC Report
Fri 27 May 2016
SRR2139096_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139096_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047980
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28700.045.9113581
CTTATAC30950.037.6296541
GTATCAA51750.037.2894361
TATACAC39900.031.2068713
ATCAACG60900.031.192183
TCAACGC61850.030.7130764
CAACGCA63000.030.1524415
TATCAAC63800.029.9134162
AACGCAG64550.029.560086
TTATACA40950.029.478462
ACGCAGA73450.026.0361167
CGCAGAG74300.025.738268
ACACATC49550.024.3573576
TACACAT53700.023.8994625
ACATCTC50700.023.5534138
CACATCT54150.022.131267
GCAGAGT85900.021.9183839
CATCTCC56250.021.3071089
ATACACA65950.020.8778484
GAGTACT50150.020.00041412-13
Produced by FastQC (version 0.11.5)