FastQCFastQC Report
Fri 27 May 2016
SRR2139095_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139095_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8067379
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98550.049.7882421
GTATCAA190400.039.1473241
TCAACGC237700.030.7828484
ATCAACG238750.030.6300073
CAACGCA244800.029.8204525
TATCAAC247900.029.6376342
AACGCAG250050.029.2965456
ACGCAGA288600.025.3389597
CGCAGAG292250.025.0370668
GCAGAGT340000.021.4835199
GTGGTAT50450.020.1775131
GTACATG225700.019.8336261
AGAGTAC309400.019.45052110-11
GAGTACT205650.019.18784112-13
TACATGG227750.019.1875692
TGGTATC50550.018.7894742
CAGAGTA332750.018.23281910-11
ACATGGG232050.018.1891443
AGTACTT226900.017.66300412-13
GTACTTT229100.017.20582814-15
Produced by FastQC (version 0.11.5)