FastQCFastQC Report
Fri 27 May 2016
SRR2139094_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139094_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11829712
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160650.052.7726481
GTATCAA295950.037.1210021
TCAACGC369550.029.0347544
ATCAACG372750.028.8209823
TATCAAC382600.028.2465232
CAACGCA382100.028.0811125
AACGCAG389900.027.5521076
ACGCAGA449800.023.9208497
CGCAGAG453650.023.6709128
GTGGTAT76250.021.0532861
GCAGAGT525500.020.3455899
GTACATG344800.020.2285611
TGGTATC72000.020.166952
GAGTACT317950.019.81904212-13
TACATGG352500.019.4001352
AGAGTAC477650.018.82191710-11
ACATGGG357200.018.6318783
CAGAGTA512850.018.5097110-11
GTACTTT351650.017.8537914-15
AGTACTT347150.017.28732112-13
Produced by FastQC (version 0.11.5)