##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139094_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11829712
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59076696034527	34.0	31.0	34.0	31.0	34.0
2	32.72140581275352	34.0	31.0	34.0	31.0	34.0
3	32.79191716586169	34.0	31.0	34.0	31.0	34.0
4	36.275857518762926	37.0	37.0	37.0	35.0	37.0
5	36.20717013229063	37.0	37.0	37.0	35.0	37.0
6	36.234539775778146	37.0	37.0	37.0	35.0	37.0
7	36.18335864812263	37.0	37.0	37.0	35.0	37.0
8	36.16135777439045	37.0	37.0	37.0	35.0	37.0
9	37.880844774581156	39.0	38.0	39.0	35.0	39.0
10-11	37.81614307262932	39.0	38.0	39.0	35.0	39.0
12-13	37.783625374818925	39.0	38.0	39.0	35.0	39.0
14-15	39.15583925458202	40.0	39.0	41.0	36.0	41.0
16-17	39.1203764301278	40.0	38.0	41.0	36.0	41.0
18-19	39.006206998107814	40.0	38.5	41.0	35.5	41.0
20-21	39.06774228563319	40.0	39.0	41.0	36.0	41.0
22-23	38.98256949014912	40.0	38.0	41.0	35.0	41.0
24-25	38.87136918391767	40.0	38.0	41.0	35.0	41.0
26-27	38.818873488261076	40.0	38.0	41.0	35.0	41.0
28-29	38.70886394628067	40.0	38.0	41.0	35.0	41.0
30-31	38.636500709551406	40.0	38.0	41.0	35.0	41.0
32-33	38.50228724501616	40.0	38.0	41.0	34.0	41.0
34-35	38.27286521145419	40.0	38.0	41.0	34.0	41.0
36-37	38.53383043275366	40.0	38.0	41.0	34.0	41.0
38-39	38.62667621262548	40.0	38.0	41.0	35.0	41.0
40-41	38.61533581708947	40.0	38.0	41.0	34.5	41.0
42-43	38.543070087460436	40.0	38.0	41.0	34.0	41.0
44-45	38.46409741484142	40.0	38.0	41.0	34.0	41.0
46-47	38.386711162126915	40.0	38.0	41.0	34.0	41.0
48-49	38.31574140210971	40.0	38.0	41.0	34.0	41.0
50-51	38.23211577236892	40.0	38.0	41.0	33.0	41.0
52-53	38.128382390350005	40.0	37.0	41.0	33.0	41.0
54-55	38.0037215021437	40.0	37.0	41.0	33.0	41.0
56-57	37.8498702121513	40.0	37.0	41.0	33.0	41.0
58-59	37.6760480709971	39.5	36.0	41.0	33.0	41.0
60-61	37.50281142048209	39.0	36.0	41.0	33.0	41.0
62-63	37.28092321212934	39.0	35.0	41.0	32.0	41.0
64-65	36.993195744907034	38.5	35.0	40.0	32.0	41.0
66-67	36.669760617222224	38.0	35.0	40.0	31.0	41.0
68-69	36.35256919907191	37.0	35.0	40.0	31.0	41.0
70-71	36.01165896650355	37.0	35.0	39.5	31.0	41.0
72-73	35.69588875367953	36.0	34.0	39.0	31.0	41.0
74-75	35.366413088905766	36.0	34.0	39.0	30.5	40.0
76-77	34.444740948615305	35.0	33.5	37.0	29.5	39.0
78-79	34.69762649232766	35.0	34.0	37.0	30.0	39.0
80-81	34.50700420828889	35.0	34.0	37.0	30.0	39.0
82-83	34.25965016604056	35.0	34.0	36.0	30.0	38.5
84-85	34.004900850840535	35.0	34.0	36.0	30.0	37.0
86-87	33.80176736638707	35.0	34.0	35.5	30.0	37.0
88-89	33.599891986843275	35.0	33.0	35.0	29.5	36.5
90-91	33.42084831715522	35.0	33.0	35.0	29.0	36.0
92-93	33.29063065480321	35.0	33.0	35.0	29.0	36.0
94-95	33.23783624424	35.0	33.0	35.0	29.0	36.0
96-97	33.233452226567536	35.0	33.0	35.0	29.0	35.0
98-99	33.31920131084569	35.0	33.0	35.0	29.5	35.0
100-101	31.722500429763237	33.0	30.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	1.0
17	0.0
18	2.0
19	8.0
20	40.0
21	334.0
22	1493.0
23	5217.0
24	13318.0
25	27345.0
26	48833.0
27	77358.0
28	112344.0
29	150801.0
30	194105.0
31	243820.0
32	308234.0
33	401398.0
34	555571.0
35	802328.0
36	1340231.0
37	2575935.0
38	3299543.0
39	1585217.0
40	86235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.67908331472905	21.391143264808964	15.66093468571374	24.26883873474825
2	13.645853991898377	21.728465258190006	41.35645638593336	23.269224363978257
3	16.835446408758045	26.586928449906484	31.409077171811102	25.168547969524376
4	11.981038586832526	18.845856647868516	38.92409356815117	30.24901119714779
5	12.973100183613983	37.954822050145104	35.719847377479866	13.352230388761042
6	29.560930027949123	38.44676990786502	18.686435989253837	13.30586407493202
7	26.010293237137112	33.155904342194724	23.16396793868725	17.669834481980917
8	24.314903017081058	36.95682532254378	21.023749352477896	17.704522307897268
9	25.276953204086954	17.06845138086682	21.445718254053798	36.20887716099243
10-11	23.894759457125527	27.134399594981584	28.92793665509244	20.042904292800454
12-13	24.78624794806906	24.687145780653474	29.126865398486913	21.399740872790552
14-15	21.914519169376742	26.056240338343834	26.88999894586666	25.139241546412766
16-17	21.392382260790296	29.21174105455217	27.539708516798058	21.856168167859483
18-19	21.47848691797913	28.207752194073414	29.439003951830088	20.874756936117365
20-21	22.189551609899897	27.318293429983303	29.41886469112619	21.073290268990608
22-23	21.9327305964748	27.278754764283423	29.245486638532636	21.543028000709143
24-25	21.740008778399204	27.463481085577047	29.26342076737889	21.533089368644863
26-27	21.721815257403097	27.55390552973266	29.215474313752914	21.508804899111333
28-29	21.667695967632277	27.59325999548033	29.227923234908047	21.51112080197935
30-31	21.8369153427574	27.49066694430338	29.199382116654167	21.47303559628505
32-33	21.687543760125518	27.628797441938513	29.096808044878507	21.58685075305746
34-35	21.853438662700825	27.623547723371818	29.04649204166267	21.476521572264687
36-37	21.752685180679542	27.6166256498136	29.027886471416924	21.602802698089935
38-39	21.668863398807588	27.673703662869524	29.041618771713978	21.615814166608903
40-41	21.899556213952064	27.549814427991166	28.937445054514832	21.61318430354194
42-43	21.831466032941577	27.65739185044311	28.698979013955267	21.812163102660048
44-45	21.893630330650662	27.76461166303628	28.492261769487154	21.849496236825903
46-47	22.02661219135179	27.744145908160334	28.178074099979007	22.051167800508864
48-49	21.935119232140764	27.93180812194378	28.086187985131282	22.046884660784173
50-51	21.947666490435978	28.00118382915406	27.979356470724927	22.07179320968504
52-53	22.104438281574836	27.9281087189578	27.891197073442925	22.076255926024434
54-55	22.069416190610166	27.85675294937005	27.877113598432135	22.196717261587647
56-57	22.09883252023167	27.970009768687504	27.839977930516085	22.09117978056474
58-59	22.0294403430463	28.015538036553128	27.899508273690156	22.055513346710416
60-61	22.12727817303867	28.00842655943072	27.855431498711354	22.008863768819257
62-63	22.067981718292575	28.105543594165454	27.79576441824697	22.030710269295
64-65	22.053103078925663	28.069329972279544	27.739297632865224	22.138269315929573
66-67	22.09397441108724	28.078755589106326	27.63832917174767	22.188940828058765
68-69	22.06107946263987	28.010090469108214	27.7073529187351	22.221477149516822
70-71	22.074415217149987	27.844776466404053	27.761328905861326	22.319479410584634
72-73	22.11111370780448	27.825861547904896	27.707402393791746	22.355622350498873
74-75	22.02786189405441	27.92896227605518	27.67023020096198	22.372945628928427
76-77	22.195505224339744	27.893162832288482	27.60395835622809	22.30737358714368
78-79	22.160796710700982	27.878743785268888	27.566343703202445	22.394115800827688
80-81	22.16464603951705	27.878174800748095	27.564955288677677	22.392223871057176
82-83	22.194232915642786	27.843156367250728	27.629650414165113	22.33296030294137
84-85	22.243945776526335	27.839926002153923	27.649453588241407	22.26667463307834
86-87	22.170021795001418	27.836651801091545	27.698627977923117	22.29469842598392
88-89	22.1865748398584	27.763035210080496	27.713303736988493	22.337086213072613
90-91	22.204129092395313	27.797673102181868	27.70949091373605	22.28870689168677
92-93	22.24739949408298	27.704570337601268	27.782849485608736	22.26518068270701
94-95	22.109021456915134	27.7433119112212	27.81579252489232	22.33187410697135
96-97	22.079836266210858	27.76645685787719	27.885105813456708	22.26860106245524
98-99	22.18083452134282	28.31271489085843	27.92606012309581	21.580390464702937
100-101	20.57516223856442	31.869499149889087	25.857303829396333	21.698034782150163
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2864.0
1	2518.5
2	3171.5
3	4419.5
4	5865.0
5	8608.5
6	11041.5
7	13897.0
8	16526.0
9	17130.5
10	16009.0
11	15072.5
12	16984.5
13	22977.0
14	37237.5
15	56366.0
16	71192.5
17	78867.5
18	79477.5
19	74805.0
20	67968.0
21	61254.0
22	57958.5
23	61363.0
24	71061.0
25	86959.5
26	107644.0
27	129865.5
28	155670.0
29	184800.0
30	211867.0
31	238237.0
32	265862.5
33	292812.0
34	318911.0
35	342805.0
36	369031.0
37	393397.5
38	412504.0
39	428191.5
40	441830.0
41	456662.5
42	466308.0
43	471519.0
44	477010.5
45	481817.5
46	484459.5
47	483847.0
48	477463.5
49	467498.0
50	458050.0
51	444507.5
52	424522.0
53	402393.0
54	378641.5
55	352034.0
56	324285.0
57	293819.5
58	260783.5
59	225834.5
60	188217.0
61	153738.5
62	122712.5
63	94102.0
64	70008.0
65	52225.5
66	39503.5
67	29974.5
68	22652.5
69	16133.5
70	11323.0
71	8076.0
72	5510.5
73	3637.5
74	2400.5
75	1568.0
76	1026.5
77	644.0
78	432.0
79	298.0
80	205.0
81	141.0
82	97.0
83	63.0
84	43.0
85	31.5
86	25.5
87	20.0
88	16.5
89	14.0
90	10.0
91	7.0
92	5.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.5359873511713556E-5
2	0.004040673179533026
3	0.004962081917125286
4	2.5359873511713556E-5
5	2.5359873511713556E-5
6	2.5359873511713556E-5
7	2.5359873511713556E-5
8	0.0
9	0.0031023578595996255
10-11	0.001069341333077255
12-13	8.453291170571185E-6
14-15	7.523429141808356E-4
16-17	6.762632936456948E-5
18-19	2.5359873511713556E-5
20-21	1.2680319425699879E-5
22-23	6.213830591956132E-4
24-25	0.004156058078269186
26-27	0.021129898035976823
28-29	0.3332672691539294
30-31	0.3262361582806807
32-33	0.3376932950308661
34-35	0.17949621022408166
36-37	0.038712773150629445
38-39	0.03570743425170401
40-41	0.01273841875520864
42-43	0.01000114995800911
44-45	0.009134448403877067
46-47	0.005706978347564389
48-49	0.009077708410026378
50-51	0.009340152352480268
52-53	0.0029643188896781598
54-55	0.003609653617271959
56-57	0.0036250938987172805
58-59	0.00444867446754636
60-61	0.003038828084886793
62-63	0.001646674285026601
64-65	4.118921501594023E-4
66-67	0.006846791310526663
68-69	0.016362835960173266
70-71	0.013268595764002038
72-73	0.013965263591229696
74-75	0.0071185228505088
76-77	0.004487383949494494
78-79	0.0015159065778744154
80-81	0.001173874622051501
82-83	7.181056289026533E-4
84-85	1.122279454317802E-4
86-87	0.0
88-89	5.048297944398924E-4
90-91	0.004818618831206958
92-93	0.01842922097406192
94-95	0.001736274247343861
96-97	5.898530876508711E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	213.0
20-21	741.0
22-23	1558.0
24-25	3956.0
26-27	5104.0
28-29	8438.0
30-31	23087.0
32-33	30240.0
34-35	44231.0
36-37	61909.0
38-39	71876.0
40-41	78076.0
42-43	93207.0
44-45	111628.0
46-47	110802.0
48-49	97340.0
50-51	87024.0
52-53	77711.0
54-55	86218.0
56-57	90934.0
58-59	93055.0
60-61	92908.0
62-63	94641.0
64-65	103661.0
66-67	109813.0
68-69	106998.0
70-71	104816.0
72-73	102859.0
74-75	105130.0
76-77	106510.0
78-79	112717.0
80-81	114629.0
82-83	113175.0
84-85	116337.0
86-87	123658.0
88-89	133498.0
90-91	145565.0
92-93	154081.0
94-95	179144.0
96-97	274228.0
98-99	1074801.0
100-101	7183195.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.09698091263292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.43782250755328	75.21450852285159
2	7.229772325223539	12.160040504740111
3	1.7840273499726917	4.500939419948055
4	0.6906086807340308	2.323124201671535
5	0.3208740513361561	1.3492269485287955
6	0.17339313208273896	0.874910335148624
7	0.1003453303806673	0.5907117533586376
8	0.06404563030158951	0.4308835319208253
9	0.043986360471141156	0.3329208105261974
>10	0.15157711864940543	1.9548126085207465
>50	0.0029757626818990735	0.1648279065155656
>100	5.365592785393227E-4	0.08086779209261227
>500	3.5191334332759607E-5	0.022225664176720013
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.690658234114237E-5
2	0.0	1.690658234114237E-5	0.0	0.0	1.690658234114237E-5
3	0.0	1.690658234114237E-5	0.0	0.0	1.690658234114237E-5
4	0.0	5.9173038193998295E-5	0.0	0.0	1.690658234114237E-5
5	0.0	6.762632936456948E-5	0.0	0.0	1.690658234114237E-5
6	0.0	6.762632936456948E-5	0.0	0.0	1.690658234114237E-5
7	0.0	7.607962053514067E-5	0.0	0.0	1.690658234114237E-5
8	0.0	8.453291170571186E-5	0.0	0.0	2.5359873511713556E-5
9	0.0	2.3669215277599318E-4	0.0	0.0	3.381316468228474E-5
10-11	0.0	2.3669215277599318E-4	0.0	8.453291170571185E-6	3.381316468228474E-5
12-13	0.0	2.451454439465644E-4	0.0	8.453291170571185E-6	3.381316468228474E-5
14-15	4.226645585285592E-6	3.2122506448170504E-4	0.0	8.453291170571185E-6	4.226645585285593E-5
16-17	8.453291170571185E-6	3.2122506448170504E-4	0.0	3.803981026757033E-5	6.339968377928388E-5
18-19	8.453291170571185E-6	3.465849379934186E-4	0.0	6.762632936456949E-5	8.030626612042627E-5
20-21	8.453291170571185E-6	3.8885139384627456E-4	0.0	1.0143949404685422E-4	8.453291170571186E-5
22-23	8.453291170571185E-6	4.6493101438141525E-4	0.0	3.8039810267570333E-4	9.721284846156864E-5
24-25	1.2679936755856778E-5	5.071974702342711E-4	0.0	0.0012849002579268204	1.0989278521742541E-4
26-27	1.690658234114237E-5	5.241040525754135E-4	0.0	0.0036771816591984656	1.0989278521742541E-4
28-29	1.690658234114237E-5	5.325573437459847E-4	0.0	0.018800119563350315	1.14119430802711E-4
30-31	1.690658234114237E-5	6.33996837792839E-4	0.0	0.026678586934322658	1.1834607638799659E-4
32-33	4.226645585285593E-5	6.551300657192669E-4	0.0	0.030423394922885698	1.3525265872913898E-4
34-35	5.4946392608712704E-5	7.734761421072635E-4	0.0	0.035393930131181556	1.5215924107028135E-4
36-37	6.762632936456948E-5	8.791422817394034E-4	0.0	0.043318890603592036	1.5215924107028135E-4
38-39	6.762632936456948E-5	9.087288008364024E-4	0.0	0.05686951635001765	1.6906582341142372E-4
40-41	6.762632936456948E-5	0.0010397548139802557	0.0	0.08025977301898812	1.6906582341142372E-4
42-43	6.762632936456948E-5	0.001069341333077255	0.0	0.09266075116621605	1.6906582341142372E-4
44-45	6.762632936456948E-5	0.0012088206373916796	0.0	0.11010411749668969	1.732924689967093E-4
46-47	6.762632936456948E-5	0.0013313933593649616	0.0	0.13115703915699722	1.9019905133785167E-4
48-49	6.762632936456948E-5	0.0013356200049502473	0.0	0.14942882802218685	2.1133227926427964E-4
50-51	6.762632936456948E-5	0.0014412861445823873	0.0	0.1701562979724274	2.240122160201364E-4
52-53	8.453291170571186E-5	0.0014581927269235295	0.0	0.19219402805410646	2.409187983612788E-4
54-55	8.453291170571186E-5	0.0014962325371910999	0.0	0.25591493689787204	2.6205202628770674E-4
56-57	8.453291170571186E-5	0.001546952284214527	0.0	0.3043269354317333	2.916385453847059E-4
58-59	8.453291170571186E-5	0.001568085512140955	0.0	0.34176233538060774	3.043184821405627E-4
60-61	8.453291170571186E-5	0.0016103519679938109	0.0	0.3630350426113501	3.1277177331113387E-4
62-63	8.453291170571186E-5	0.0016483917782613813	0.0	0.38081654058864667	3.296783556522762E-4
64-65	9.298620287628304E-5	0.0019019905133785167	0.0	0.3895022972664085	3.296783556522762E-4
66-67	9.298620287628304E-5	0.0019188970957196591	0.0	0.3951533224139353	3.296783556522762E-4
68-69	9.298620287628304E-5	0.0019611635515725152	0.0	0.40109175946126163	3.3813164682284743E-4
70-71	9.298620287628304E-5	0.0019949767162548	0.0	0.4064046529619656	3.7194481150513216E-4
72-73	9.721284846156864E-5	0.002007656653010657	0.0	0.4108257242441743	4.057579761874169E-4
74-75	1.0143949404685422E-4	0.0020668296912046548	0.0	0.41468465166354007	4.057579761874169E-4
76-77	1.0143949404685422E-4	0.002151362602910367	0.0	0.4180532881950127	4.057579761874169E-4
78-79	1.0143949404685422E-4	0.002206308995519079	0.0	0.4209908068767862	4.057579761874169E-4
80-81	1.0143949404685422E-4	0.002206308995519079	0.0	0.42220808080534844	4.142112673579881E-4
82-83	1.0143949404685422E-4	0.002210535641104365	0.0	0.4226349720094623	4.4802443204027285E-4
84-85	1.0989278521742541E-4	0.002240122160201364	0.0	0.42294774378277344	4.4802443204027285E-4
86-87	1.0989278521742541E-4	0.002290841907224791	0.0	0.42308722308708785	4.56477723210844E-4
88-89	1.0989278521742541E-4	0.0024556810850509293	0.0	0.42313794283411127	4.6915765996670075E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16065	0.0	52.772648	1
GTATCAA	29595	0.0	37.121002	1
TCAACGC	36955	0.0	29.034754	4
ATCAACG	37275	0.0	28.820982	3
TATCAAC	38260	0.0	28.246523	2
CAACGCA	38210	0.0	28.081112	5
AACGCAG	38990	0.0	27.552107	6
ACGCAGA	44980	0.0	23.920849	7
CGCAGAG	45365	0.0	23.670912	8
GTGGTAT	7625	0.0	21.053286	1
GCAGAGT	52550	0.0	20.345589	9
GTACATG	34480	0.0	20.228561	1
TGGTATC	7200	0.0	20.16695	2
GAGTACT	31795	0.0	19.819042	12-13
TACATGG	35250	0.0	19.400135	2
AGAGTAC	47765	0.0	18.821917	10-11
ACATGGG	35720	0.0	18.631878	3
CAGAGTA	51285	0.0	18.50971	10-11
GTACTTT	35165	0.0	17.85379	14-15
AGTACTT	34715	0.0	17.287321	12-13
>>END_MODULE