FastQCFastQC Report
Fri 27 May 2016
SRR2139093_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139093_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181557
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27550.049.2486461
GTATCAA54900.038.6828271
ATCAACG67450.030.8571073
TCAACGC68050.030.6469524
CAACGCA69250.030.1158895
TATCAAC71150.029.6111372
AACGCAG71150.029.4893156
ACGCAGA82400.025.3609037
CGCAGAG82800.025.2383868
CTTATAC27900.023.409251
GCAGAGT95250.021.7645749
TATACAC36150.021.6777823
TACATGG64200.020.6745242
GTACATG64900.020.5813831
AGAGTAC86600.019.99742110-11
GAGTACT57750.019.44443312-13
ACATGGG65900.019.435623
TTATACA37800.018.3929822
CAGAGTA92900.018.21041710-11
ACCGAAC3051.036824E-917.9578258
Produced by FastQC (version 0.11.5)