FastQCFastQC Report
Fri 27 May 2016
SRR2139092_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139092_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13865693
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA175200.052.0115551
GTATCAA325750.037.0946731
TCAACGC410350.028.7243164
ATCAACG411900.028.6267073
CAACGCA423900.027.7961545
TATCAAC428850.027.7128752
AACGCAG435600.027.1175796
ACGCAGA498400.023.6752017
CGCAGAG515450.022.8756528
GTACATG415250.020.0690121
GCAGAGT587300.019.8826969
GTGGTAT87100.019.5342981
TACATGG418500.019.458062
GAGTACT330850.018.9445412-13
AGAGTAC531650.018.92211710-11
ACATGGG424100.018.671863
CTTATAC158300.018.6622681
CAGAGTA570450.017.97267210-11
TGGTATC87150.017.9690132
TATACAC205450.017.2425443
Produced by FastQC (version 0.11.5)