FastQCFastQC Report
Fri 27 May 2016
SRR2139091_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139091_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13370821
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA189950.049.4107171
GTATCAA338550.035.0248831
TCAACGC413000.028.0628874
ATCAACG413900.027.9300963
CAACGCA422100.027.457685
TATCAAC427650.027.2828522
AACGCAG434550.026.7197276
CTTATAC172950.026.3640861
ACGCAGA495500.023.3903757
CGCAGAG507500.022.83738
TATACAC233200.022.1227383
GTACATG382650.020.9165531
TTATACA232300.020.5197332
TACATGG391500.019.9687942
GCAGAGT582900.019.767399
GAGTACT349150.019.40568512-13
ACATGGG393650.019.3203073
GTGGTAT95350.018.8799461
AGAGTAC537100.018.3738110-11
CAGAGTA570950.018.1540710-11
Produced by FastQC (version 0.11.5)