##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139091_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13370821
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5828139498689	34.0	31.0	34.0	31.0	34.0
2	32.719414462283204	34.0	31.0	34.0	31.0	34.0
3	32.70557739124621	34.0	31.0	34.0	31.0	34.0
4	36.22348575304389	37.0	35.0	37.0	35.0	37.0
5	36.18017173365794	37.0	35.0	37.0	35.0	37.0
6	36.222320304789065	37.0	37.0	37.0	35.0	37.0
7	36.181631180314206	37.0	37.0	37.0	35.0	37.0
8	36.15890542547836	37.0	37.0	37.0	35.0	37.0
9	37.89075053805597	39.0	38.0	39.0	35.0	39.0
10-11	37.821072580359875	39.0	38.0	39.0	35.0	39.0
12-13	37.78999142984563	39.0	38.0	39.0	35.0	39.0
14-15	39.13835317217993	40.0	39.0	41.0	36.0	41.0
16-17	39.10673720035591	40.0	38.0	41.0	36.0	41.0
18-19	39.07014191574324	40.0	38.5	41.0	36.0	41.0
20-21	39.0584940196664	40.0	39.0	41.0	35.5	41.0
22-23	38.9398149893654	40.0	38.0	41.0	35.0	41.0
24-25	38.79944355661931	40.0	38.0	41.0	35.0	41.0
26-27	38.74469361787649	40.0	38.0	41.0	34.5	41.0
28-29	38.686813895895455	40.0	38.0	41.0	35.0	41.0
30-31	38.61743354783678	40.0	38.0	41.0	35.0	41.0
32-33	38.50107105067493	40.0	38.0	41.0	34.0	41.0
34-35	38.305750561139405	40.0	38.0	41.0	34.0	41.0
36-37	38.43736632157991	40.0	38.0	41.0	34.0	41.0
38-39	38.40598505261768	40.0	38.0	41.0	34.0	41.0
40-41	38.38443114910528	40.0	38.0	41.0	34.0	41.0
42-43	38.42462477776863	40.0	38.0	41.0	34.0	41.0
44-45	38.3642105584229	40.0	38.0	41.0	34.0	41.0
46-47	38.3023963062201	40.0	38.0	41.0	33.5	41.0
48-49	38.14611553498054	40.0	38.0	41.0	33.0	41.0
50-51	38.13486467168943	40.0	37.0	41.0	33.0	41.0
52-53	38.040299117162604	40.0	37.0	41.0	33.0	41.0
54-55	37.89102731336731	40.0	37.0	41.0	33.0	41.0
56-57	37.709347408263525	40.0	36.5	41.0	33.0	41.0
58-59	37.53870200096801	39.0	36.0	41.0	33.0	41.0
60-61	37.350814392466674	39.0	35.5	41.0	32.0	41.0
62-63	37.136963613994006	39.0	35.0	40.5	32.0	41.0
64-65	36.84283199514937	38.0	35.0	40.0	31.5	41.0
66-67	36.48681872387573	38.0	35.0	40.0	31.0	41.0
68-69	36.15347294190114	37.0	35.0	40.0	31.0	41.0
70-71	35.85409899294518	36.5	34.5	39.0	31.0	41.0
72-73	35.528943249393656	36.0	34.0	39.0	30.0	40.5
74-75	35.16212155567439	36.0	34.0	38.5	30.0	40.0
76-77	34.208168596105544	35.0	33.0	37.0	29.0	39.0
78-79	34.488532774213695	35.0	34.0	37.0	30.0	39.0
80-81	34.29862287991088	35.0	34.0	36.5	30.0	39.0
82-83	34.03308466722939	35.0	34.0	36.0	29.0	38.0
84-85	33.79534694205806	35.0	33.0	36.0	29.0	37.0
86-87	33.565138965097745	35.0	33.0	35.0	29.0	37.0
88-89	33.33815142328853	35.0	33.0	35.0	29.0	36.0
90-91	33.15863385426999	35.0	33.0	35.0	29.0	36.0
92-93	33.07359843032574	35.0	33.0	35.0	29.0	36.0
94-95	33.00006931388394	34.0	33.0	35.0	29.0	35.5
96-97	32.98215987603043	34.0	32.0	35.0	29.0	35.0
98-99	33.10192391836699	34.0	32.5	35.0	29.0	35.0
100-101	31.219411370666982	32.5	29.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	3.0
19	10.0
20	65.0
21	407.0
22	1900.0
23	6342.0
24	16097.0
25	33414.0
26	59541.0
27	92697.0
28	131880.0
29	177360.0
30	228415.0
31	288037.0
32	364569.0
33	476092.0
34	662530.0
35	961140.0
36	1602868.0
37	2971143.0
38	3522115.0
39	1674118.0
40	100075.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.64437633593153	21.3799677207531	15.458715321872555	24.516940621442814
2	13.691954492774133	21.557325583535007	41.510099127983125	23.24062079570773
3	16.92133317178752	26.583268640218026	31.51455183750884	24.98084635048561
4	12.06861310232844	18.917186624374505	38.40460843065549	30.609591842641564
5	12.895641404192116	38.01205161687914	35.809980240553685	13.282326738375058
6	29.63222150922681	38.32429873410905	18.49911972489346	13.544360031770678
7	25.969020598587072	33.110923638191224	22.948884212037857	17.971171551183847
8	24.33871487771768	36.97382531708412	21.047697819004533	17.639761986193665
9	25.2283760286672	16.80900522114536	21.294825500992047	36.6677932491954
10-11	23.944618280937824	26.932546753739977	28.92161218152808	20.20122278379412
12-13	24.845376931615085	24.474810333997198	29.040021042962387	21.63979169142533
14-15	21.99764882888127	25.896530296822174	26.64525979348929	25.46056108080727
16-17	21.454171621421352	29.185148327022148	27.33568778215177	22.024992269404727
18-19	21.536669289043658	28.151831514310153	29.240003287756227	21.07149590888996
20-21	22.244991861492117	27.16282258696027	29.184476859196373	21.40770869235124
22-23	22.02168044302148	27.343977433645662	29.006949811223997	21.62739231210886
24-25	21.91103913009434	27.642817943824454	28.994974801053218	21.45116812502799
26-27	21.847122483852313	27.738581111497506	29.006299101960725	21.407997302689456
28-29	21.704972022616342	27.7126622104979	29.006405108608078	21.57596065827768
30-31	22.10603426047847	27.46179432800475	28.99754184283911	21.434629568677668
32-33	21.7836140229422	27.62133480962557	29.021081445618226	21.573969721814006
34-35	21.88602542276481	27.42674308499491	29.09607876027523	21.591152731965053
36-37	21.7437295431855	27.65621020938349	28.9671390214204	21.632921226010605
38-39	21.801686816327003	27.422300354011657	29.090350577999402	21.685662251661935
40-41	21.849106345812356	27.302209370782037	29.056135296702283	21.792548986703327
42-43	21.953274825410848	27.48242524733117	28.803450317129958	21.76084961012802
44-45	21.88691409756438	27.672190267748498	28.59596139364864	21.84493424103848
46-47	22.123589708810005	27.458967917052597	28.17982529080641	22.23761708333099
48-49	22.022263905122415	27.712562783388822	28.029041625253626	22.23613168623514
50-51	21.892990928296967	27.716878889578933	28.18504473102226	22.205085451101844
52-53	22.01430861819018	27.74237535833179	28.163538727719573	22.07977729575846
54-55	21.984316322954307	27.73848762000675	27.987120554213764	22.290075502825182
56-57	22.230830115948248	27.87410117634506	27.729923063253686	22.165145644453002
58-59	21.8617058915043	28.212190309257245	28.054718630883492	21.871385168354955
60-61	22.209772893912145	28.320409537778286	27.620321127637286	21.84949644067228
62-63	22.007585642636357	28.540194645903018	27.533495203355823	21.918724508104802
64-65	21.93579313755521	28.392954622849604	27.606726229488558	22.06452601010663
66-67	22.09462019239768	28.2236610878226	27.48080979776546	22.20090892201426
68-69	21.99173578999446	28.06942750949794	27.63641513591647	22.30242156459113
70-71	21.977416116194394	27.920767508436604	27.695816558717944	22.40599981665106
72-73	22.108205919666382	27.867603643085687	27.639761547236436	22.3844288900115
74-75	22.02122087511718	27.95206856201239	27.625238550376096	22.401472012494335
76-77	22.170141970167386	27.897208801712527	27.565450772848326	22.367198455271758
78-79	22.126440651363183	27.813832017694374	27.560378610538518	22.499348720403926
80-81	22.13067362887552	27.883810076286174	27.543502952151023	22.442013342687286
82-83	22.112444918343662	27.882783282702416	27.68446788965048	22.320303909303437
84-85	22.28042592314651	27.895842376150608	27.561030784922085	22.26270091578079
86-87	22.1514330826427	27.842401190745864	27.646408859236875	22.35975686737456
88-89	22.144527364567477	27.79627502963356	27.663301377285283	22.39589622851368
90-91	22.19637751951359	27.80672175843541	27.645306920889567	22.35159380116143
92-93	22.304428178471774	27.660745415139964	27.764985621996125	22.269840784392137
94-95	22.036851153971842	27.730804729846813	27.858714002138484	22.37363011404286
96-97	21.96108349100191	27.732959091140046	27.943271175887514	22.36268624197053
98-99	22.122888311086285	28.29516159890375	27.942715717629756	21.639234372380205
100-101	20.72701897792654	31.83769888578572	25.686033929459146	21.749248206828593
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3283.0
1	2965.0
2	3919.5
3	5474.5
4	7326.0
5	11040.0
6	14268.0
7	17684.5
8	20747.0
9	21379.5
10	19721.0
11	17882.5
12	19530.5
13	26277.0
14	42725.5
15	65024.0
16	82207.0
17	90777.5
18	91273.0
19	85704.5
20	77281.0
21	69451.5
22	65465.0
23	69762.0
24	80693.5
25	97854.5
26	121428.0
27	145498.0
28	171036.0
29	199732.5
30	228436.0
31	258628.0
32	291335.0
33	324404.5
34	354986.0
35	381753.0
36	413616.0
37	444267.0
38	464703.0
39	478959.0
40	491945.0
41	506956.5
42	517597.5
43	528485.5
44	542417.5
45	555747.5
46	573215.0
47	586076.5
48	577469.0
49	562365.0
50	546279.0
51	521594.5
52	494422.5
53	466137.5
54	438209.5
55	406201.0
56	372551.0
57	337860.5
58	299053.5
59	256471.5
60	211584.5
61	170758.0
62	134532.5
63	102704.0
64	76825.0
65	57756.0
66	44328.5
67	33847.5
68	25812.5
69	18791.0
70	13091.5
71	9183.0
72	6440.5
73	4472.5
74	2939.0
75	1904.0
76	1265.5
77	808.5
78	529.5
79	352.0
80	240.0
81	160.0
82	107.0
83	64.0
84	40.0
85	34.0
86	27.5
87	21.0
88	16.0
89	10.0
90	5.5
91	4.5
92	3.5
93	1.5
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.4789723084319205E-6
2	0.015279540426126415
3	7.4789723084319205E-6
4	1.6453739078550224E-4
5	7.4789723084319205E-6
6	7.4789723084319205E-6
7	7.4789723084319205E-6
8	0.0
9	0.0
10-11	1.4210047386020648E-4
12-13	5.983177846745536E-4
14-15	2.80461461566197E-4
16-17	0.0018435666740284685
18-19	0.0
20-21	0.0
22-23	1.8699545354213893E-4
24-25	0.009524011525625072
26-27	0.019586698214448302
28-29	0.3452735765187011
30-31	0.32128007969050365
32-33	0.31534751997707494
34-35	0.18488925373748522
36-37	0.044981672542280936
38-39	0.036551398405102575
40-41	0.014943015334162844
42-43	0.013782749811471671
44-45	0.017081227606208716
46-47	0.011413636769196153
48-49	0.006717325636311589
50-51	0.0037126955785684763
52-53	0.001483217994610867
54-55	1.4252019022454828E-4
56-57	3.4086485874683457E-4
58-59	0.0013223333986347716
60-61	0.0011303910705134181
62-63	2.7919730099123076E-4
64-65	1.1978718266877972E-4
66-67	0.003310040009891994
68-69	0.005542637363273932
70-71	0.007107041004563599
72-73	0.01112560017204962
74-75	0.008099642023048269
76-77	0.006090090381163474
78-79	0.0039748532949444235
80-81	0.00490837396337046
82-83	0.0016858167191492208
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.002554062847713448
92-93	0.01618853527931519
94-95	0.0013849957015294391
96-97	1.0764808420319266E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	276.0
20-21	845.0
22-23	1946.0
24-25	5244.0
26-27	6257.0
28-29	10382.0
30-31	28082.0
32-33	37146.0
34-35	53625.0
36-37	72865.0
38-39	85803.0
40-41	91862.0
42-43	109039.0
44-45	130267.0
46-47	128497.0
48-49	112094.0
50-51	101578.0
52-53	90920.0
54-55	100392.0
56-57	112235.0
58-59	118625.0
60-61	122790.0
62-63	129126.0
64-65	139415.0
66-67	153741.0
68-69	133044.0
70-71	123778.0
72-73	121135.0
74-75	124195.0
76-77	125191.0
78-79	131745.0
80-81	135992.0
82-83	134623.0
84-85	139894.0
86-87	149374.0
88-89	160702.0
90-91	173723.0
92-93	189205.0
94-95	226054.0
96-97	342015.0
98-99	1266897.0
100-101	7750202.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.21401003497944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.41673302070083	72.69094175823997
2	7.572580378494054	12.451443984563971
3	2.09754001801405	5.173415282693343
4	0.8416490600606149	2.7678137707901764
5	0.39894141588963344	1.6399286784659615
6	0.22726033725701186	1.121039018868047
7	0.12961857496408102	0.7459523975971684
8	0.07539105700598363	0.49585608937901154
9	0.05981892933394763	0.44261586508886075
>10	0.17673836999910686	2.2094390328737217
>50	0.0031461073513428047	0.17301019034068554
>100	5.49309837489969E-4	0.07229141992613743
>500	3.3421091781972306E-5	0.016252511172949048
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.4789723084319205E-6
2	0.0	2.9915889233727682E-5	0.0	0.0	7.4789723084319205E-6
3	0.0	2.9915889233727682E-5	0.0	0.0	7.4789723084319205E-6
4	0.0	1.2714252924334266E-4	0.0	0.0	7.4789723084319205E-6
5	0.0	1.3462150155177456E-4	0.0	0.0	7.4789723084319205E-6
6	0.0	1.3462150155177456E-4	0.0	0.0	2.243691692529576E-5
7	0.0	1.4210047386020648E-4	0.0	0.0	3.73948615421596E-5
8	0.0	1.7201636309393417E-4	0.0	0.0	3.73948615421596E-5
9	0.0	2.617640307951172E-4	0.0	0.0	4.487383385059152E-5
10-11	0.0	2.617640307951172E-4	0.0	0.0	4.487383385059152E-5
12-13	0.0	2.692430031035491E-4	0.0	3.7394861542159603E-6	4.861332000480749E-5
14-15	0.0	3.4777221234208434E-4	0.0	1.121845846264788E-5	5.60922923132394E-5
16-17	0.0	3.589906708047322E-4	0.0	2.9915889233727682E-5	5.9831778467455364E-5
18-19	0.0	4.038645046553237E-4	0.0	4.487383385059152E-5	7.105023693010324E-5
20-21	0.0	4.936121723565067E-4	0.0	1.682768769397182E-4	8.226869539275112E-5
22-23	0.0	5.422254923613142E-4	0.0	4.898726862022908E-4	8.974766770118305E-5
24-25	0.0	6.207547015998495E-4	0.0	0.0014397021693731446	1.1966355693491073E-4
26-27	0.0	6.61889049296225E-4	0.0	0.003926460461926758	1.1966355693491073E-4
28-29	0.0	6.805864800673048E-4	0.0	0.020757887642052796	1.1966355693491073E-4
30-31	7.4789723084319205E-6	7.703341477684878E-4	0.0	0.02932878990751578	1.1966355693491073E-4
32-33	7.4789723084319205E-6	8.077290093106474E-4	0.0	0.03229420242780903	1.2714252924334266E-4
34-35	7.4789723084319205E-6	9.348715385539901E-4	0.0	0.03629919209897432	1.3462150155177456E-4
36-37	7.4789723084319205E-6	0.0010470561231804688	0.0	0.04317236765042326	1.3462150155177456E-4
38-39	7.4789723084319205E-6	0.0010844509847226283	0.0	0.05656720705482483	1.5705841847707034E-4
40-41	7.4789723084319205E-6	0.0011891565970406754	0.0	0.0821939056696668	1.6453739078550224E-4
42-43	7.4789723084319205E-6	0.001230290944737051	0.0	0.09395085013852178	1.6453739078550224E-4
44-45	7.4789723084319205E-6	0.0013798703909056894	0.0	0.11165357759258014	1.6453739078550224E-4
46-47	7.4789723084319205E-6	0.0014957944616863842	0.0	0.13419146064403972	1.682768769397182E-4
48-49	7.4789723084319205E-6	0.001514491892457464	0.0	0.15305716829205926	1.7201636309393417E-4
50-51	7.4789723084319205E-6	0.0016229369909297267	0.0	0.17644765418668007	1.7575584924815014E-4
52-53	7.4789723084319205E-6	0.0016640713386261025	0.0	0.20300548485392184	1.86974307710798E-4
54-55	7.4789723084319205E-6	0.0017089451724766939	0.0	0.2837559488680613	2.0193225232766185E-4
56-57	7.4789723084319205E-6	0.0017463400340188536	0.0	0.35074136434853176	2.2810865540717358E-4
58-59	7.4789723084319205E-6	0.001791213867869445	0.0	0.40406269742149714	2.3932711386982146E-4
60-61	7.4789723084319205E-6	0.0018398271878742524	0.0	0.4270081844637663	2.3932711386982146E-4
62-63	7.4789723084319205E-6	0.0018697430771079803	0.0	0.4397373953327174	2.3932711386982146E-4
64-65	7.4789723084319205E-6	0.0020866332740525056	0.0	0.44929926142904764	2.3932711386982146E-4
66-67	7.4789723084319205E-6	0.002138986080211529	0.0	0.4551478177742414	2.430666000240374E-4
68-69	7.4789723084319205E-6	0.002183859914062121	0.0	0.461011332064052	2.542850584866853E-4
70-71	7.4789723084319205E-6	0.0022062968309874164	0.0	0.46639619212612304	2.542850584866853E-4
72-73	7.4789723084319205E-6	0.0022062968309874164	0.0	0.4710069785542713	2.8420094772041297E-4
74-75	7.4789723084319205E-6	0.0022399522063753603	0.0	0.47498205233620283	2.8420094772041297E-4
76-77	7.4789723084319205E-6	0.0023110024433054635	0.0	0.47822418683190804	2.916799200288449E-4
78-79	7.4789723084319205E-6	0.0023259603879223273	0.0	0.4812569101029772	2.916799200288449E-4
80-81	7.4789723084319205E-6	0.002329699874076543	0.0	0.48261434357695765	2.991588923372768E-4
82-83	7.4789723084319205E-6	0.0023446578186934073	0.0	0.48315282958316474	3.141168369541407E-4
84-85	7.4789723084319205E-6	0.002370834221772919	0.0	0.483537996657049	3.141168369541407E-4
86-87	7.4789723084319205E-6	0.002397010624852431	0.0	0.4837100130201429	3.178563231083566E-4
88-89	7.4789723084319205E-6	0.002483018806399398	0.0	0.48376984479861035	3.253352954167885E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18995	0.0	49.410717	1
GTATCAA	33855	0.0	35.024883	1
TCAACGC	41300	0.0	28.062887	4
ATCAACG	41390	0.0	27.930096	3
CAACGCA	42210	0.0	27.45768	5
TATCAAC	42765	0.0	27.282852	2
AACGCAG	43455	0.0	26.719727	6
CTTATAC	17295	0.0	26.364086	1
ACGCAGA	49550	0.0	23.390375	7
CGCAGAG	50750	0.0	22.8373	8
TATACAC	23320	0.0	22.122738	3
GTACATG	38265	0.0	20.916553	1
TTATACA	23230	0.0	20.519733	2
TACATGG	39150	0.0	19.968794	2
GCAGAGT	58290	0.0	19.76739	9
GAGTACT	34915	0.0	19.405685	12-13
ACATGGG	39365	0.0	19.320307	3
GTGGTAT	9535	0.0	18.879946	1
AGAGTAC	53710	0.0	18.37381	10-11
CAGAGTA	57095	0.0	18.15407	10-11
>>END_MODULE