FastQCFastQC Report
Fri 27 May 2016
SRR2139090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15042281
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA189800.050.115941
GTATCAA357250.038.271381
TCAACGC441950.030.2634184
ATCAACG442750.030.1416653
TATCAAC460300.029.3087732
CAACGCA456600.029.273825
AACGCAG468050.028.6121126
ACGCAGA542950.024.6417357
CGCAGAG551500.024.2597088
GCAGAGT633200.021.0030339
GTGGTAT92700.020.7772831
GTACATG433350.020.0980321
TGGTATC90750.019.9205272
TACATGG429300.019.6776312
GAGTACT387550.018.99437312-13
ACATGGG438200.018.9088613
AGAGTAC582100.018.73703810-11
CAGAGTA619750.018.15414810-11
GTACTTT430650.017.04951714-15
AGTACTT425500.016.90325712-13
Produced by FastQC (version 0.11.5)