##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139090_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15042281
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55270666729334	34.0	31.0	34.0	31.0	34.0
2	32.69602974442506	34.0	31.0	34.0	31.0	34.0
3	32.683249102978465	34.0	31.0	34.0	31.0	34.0
4	36.2110743044888	37.0	35.0	37.0	35.0	37.0
5	36.16610452896074	37.0	35.0	37.0	35.0	37.0
6	36.20896850683749	37.0	37.0	37.0	35.0	37.0
7	36.16905248612228	37.0	37.0	37.0	35.0	37.0
8	36.14557566103173	37.0	37.0	37.0	35.0	37.0
9	37.87312323177582	39.0	38.0	39.0	35.0	39.0
10-11	37.80181682552001	39.0	38.0	39.0	35.0	39.0
12-13	37.772857088629046	39.0	38.0	39.0	35.0	39.0
14-15	39.11934695941393	40.0	38.5	41.0	36.0	41.0
16-17	39.085736199184154	40.0	38.0	41.0	36.0	41.0
18-19	39.04602227547804	40.0	38.5	41.0	36.0	41.0
20-21	39.02976400882278	40.0	39.0	41.0	35.5	41.0
22-23	38.910272793081866	40.0	38.0	41.0	35.0	41.0
24-25	38.77298362646272	40.0	38.0	41.0	35.0	41.0
26-27	38.7230902258639	40.0	38.0	41.0	34.5	41.0
28-29	38.6712916135554	40.0	38.0	41.0	35.0	41.0
30-31	38.604702459063404	40.0	38.0	41.0	34.5	41.0
32-33	38.492213848157846	40.0	38.0	41.0	34.0	41.0
34-35	38.30011717913916	40.0	38.0	41.0	34.0	41.0
36-37	38.433967952359424	40.0	38.0	41.0	34.0	41.0
38-39	38.41247005117221	40.0	38.0	41.0	34.0	41.0
40-41	38.38311581244106	40.0	38.0	41.0	34.0	41.0
42-43	38.41626968341632	40.0	38.0	41.0	34.0	41.0
44-45	38.35378860638096	40.0	38.0	41.0	34.0	41.0
46-47	38.293574420785546	40.0	38.0	41.0	33.5	41.0
48-49	38.13887548509637	40.0	38.0	41.0	33.0	41.0
50-51	38.13629463989133	40.0	37.5	41.0	33.0	41.0
52-53	38.04305428752642	40.0	37.0	41.0	33.0	41.0
54-55	37.8959128849184	40.0	37.0	41.0	33.0	41.0
56-57	37.72676602004496	40.0	36.5	41.0	33.0	41.0
58-59	37.56288559654888	39.0	36.0	41.0	33.0	41.0
60-61	37.3843444549244	39.0	36.0	41.0	32.0	41.0
62-63	37.17480980720688	39.0	35.0	40.5	32.0	41.0
64-65	36.883645837874965	38.0	35.0	40.0	31.5	41.0
66-67	36.52843815881846	38.0	35.0	40.0	31.0	41.0
68-69	36.19583877316293	37.0	35.0	40.0	31.0	41.0
70-71	35.892040589498535	37.0	34.5	39.0	31.0	41.0
72-73	35.56464236388047	36.0	34.0	39.0	30.0	40.5
74-75	35.19680473914377	36.0	34.0	39.0	30.0	40.0
76-77	34.229790662170245	35.0	33.0	37.0	29.0	39.0
78-79	34.517069711711585	35.0	34.0	37.0	29.5	39.0
80-81	34.3195393919577	35.0	34.0	37.0	29.5	39.0
82-83	34.04150527062854	35.0	33.5	36.0	29.0	38.5
84-85	33.79313961655835	35.0	33.0	36.0	29.0	37.0
86-87	33.55019235157747	35.0	33.0	35.5	29.0	37.0
88-89	33.309075906434806	35.0	33.0	35.0	29.0	36.5
90-91	33.119534532897475	35.0	33.0	35.0	29.0	36.0
92-93	33.026403674081095	34.5	33.0	35.0	29.0	36.0
94-95	32.94556908523466	34.0	32.0	35.0	29.0	35.5
96-97	32.92468951844302	34.0	32.0	35.0	29.0	35.0
98-99	33.05034049820351	34.0	32.0	35.0	29.0	35.0
100-101	31.17299553304975	32.5	28.5	34.5	24.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	5.0
19	11.0
20	86.0
21	417.0
22	2132.0
23	7145.0
24	18025.0
25	37475.0
26	65745.0
27	104784.0
28	148799.0
29	200116.0
30	258051.0
31	325959.0
32	412294.0
33	539325.0
34	747990.0
35	1084276.0
36	1801537.0
37	3307780.0
38	3956296.0
39	1913605.0
40	110426.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.33043516876665	21.690536387471607	15.585244762445905	24.393783681315842
2	13.56921650359048	21.828079609290032	41.60927766621899	22.993426220900492
3	16.8417897314629	26.78140061090477	31.5271642016479	24.84964545598443
4	11.940329465010754	18.94269912144215	38.92433087703215	30.192640536514947
5	12.735357454811203	38.15952356687441	36.003068418023624	13.102050560290765
6	29.428324695458404	38.588558383436556	18.747949114097064	13.235167807007981
7	25.78714131102466	33.174957519737696	23.38818982228758	17.649711346950063
8	24.0057275887879	37.32832806407485	21.22639511919768	17.439549227939565
9	25.145847228887693	16.952994030626073	21.651709604414386	36.249449136071846
10-11	23.694425868078415	27.214149282007895	29.184607646928622	19.90681720298507
12-13	24.523759379122158	24.76775649496689	29.3466754100767	21.361808715834254
14-15	21.671736445892915	26.172372803929893	27.03997965196605	25.115911098211143
16-17	21.1757350783246	29.38567518230888	27.742408075050996	21.696181664315525
18-19	21.237224593796647	28.435341687873	29.553519841837815	20.773913876492532
20-21	21.963184677933246	27.512161913755868	29.551763465029367	20.972889943281523
22-23	21.674794099314358	27.584465406191566	29.345186785788492	21.395553708705588
24-25	21.503273040044125	27.755712768951273	29.395878979270794	21.345135211733805
26-27	21.478440336329477	27.937972585735775	29.324484675121298	21.259102402813447
28-29	21.371393114701196	27.953391118652686	29.329417726577685	21.34579804006843
30-31	21.725311331157673	27.744667257289475	29.285357880888956	21.244663530663896
32-33	21.455161422389953	27.887613750644075	29.247891604666336	21.40933322229963
34-35	21.59740779746181	27.876611094746874	29.227697095209397	21.29828401258192
36-37	21.48489352707946	27.962671870048673	29.169019682789806	21.38341492008206
38-39	21.45736915171525	27.902003790889836	29.23396634040955	21.406660716985364
40-41	21.639041089213094	27.779560906335004	29.108363987199382	21.47303401725252
42-43	21.588542762270492	27.87217846150993	28.936174231724323	21.60310454449526
44-45	21.614867044901292	28.028145186890846	28.739036026794434	21.617951741413428
46-47	21.74002160780156	27.94424681515006	28.42729334777589	21.888438229272495
48-49	21.73250821791723	28.06328448860032	28.333394996746225	21.87081229673623
50-51	21.670099829659208	28.19330459170756	28.294238691571476	21.842356887061754
52-53	21.800057875116952	28.126662902337827	28.25255063497148	21.820728587573743
54-55	21.763257696518558	28.083114066813447	28.193299820267125	21.960328416400866
56-57	21.86244114653351	28.16169378798629	28.074802174151237	21.901062891328962
58-59	21.68426115897274	28.31028975277741	28.245107294281606	21.760341793968237
60-61	21.868986187108856	28.350105969196388	28.04626192295473	21.734645920740025
62-63	21.764580146537856	28.497334283437585	27.995719414682213	21.742366155342346
64-65	21.690678422104227	28.43022584166082	28.016215551902274	21.862880184332674
66-67	21.789873106312513	28.394721349376546	27.88191324352906	21.93349230078188
68-69	21.772712866079345	28.335987787409177	27.925312327527635	21.965987018983842
70-71	21.739687714534515	28.131980431814046	28.016162804333337	22.1121690493181
72-73	21.804672606487948	28.10317086707657	27.984366742315874	22.107789784119607
74-75	21.780041323122866	28.1602881583421	27.922580724151036	22.137089794383996
76-77	21.893127244209058	28.09881756096034	27.864593519467412	22.14346167536318
78-79	21.849492836423494	28.048577987066693	27.86727068347493	22.234658493034885
80-81	21.84300890064183	28.143010825931164	27.840042952745677	22.17393732068133
82-83	21.89754650986173	28.095734729098115	27.910757252602146	22.095961508438013
84-85	21.966773727642178	28.09508818672159	27.893024653834587	22.04511343180165
86-87	21.854983688139967	28.109352528745923	27.925526977233396	22.110136805880714
88-89	21.8564136137328	28.073535674779613	27.985705169067877	22.08434554241971
90-91	21.909311619972605	28.06289369905066	27.98795738930158	22.039837291675152
92-93	21.970724521683714	27.95722139702263	28.05966375249971	22.012390328793945
94-95	21.806218391540156	27.96292862204281	28.139209647788277	22.091643338628757
96-97	21.686306352429778	28.022610544069632	28.18710397409316	22.10397912940743
98-99	21.798080084462832	28.53062073040776	28.296068522431174	21.37523066269823
100-101	20.345516526888566	32.12370845310753	26.168935576459923	21.36183944354397
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3829.0
1	3363.5
2	4208.0
3	5886.5
4	7797.5
5	11567.5
6	14821.5
7	18354.0
8	21500.5
9	22182.5
10	20871.5
11	19429.0
12	21550.5
13	29100.5
14	46943.5
15	70879.0
16	89244.0
17	98338.5
18	99105.0
19	94051.0
20	86354.5
21	79706.5
22	77743.0
23	83745.0
24	97898.0
25	120808.5
26	150650.0
27	181983.0
28	216037.0
29	253003.5
30	287810.0
31	323100.5
32	360109.5
33	395119.5
34	427816.5
35	458488.0
36	490811.0
37	521074.0
38	544338.5
39	563235.5
40	580283.0
41	595032.5
42	603106.5
43	608100.5
44	613710.5
45	620540.5
46	629608.0
47	633363.0
48	623030.5
49	606362.0
50	589052.0
51	562723.5
52	528714.5
53	494128.0
54	461089.0
55	425767.0
56	388588.0
57	349423.0
58	306920.5
59	262592.0
60	218897.5
61	179311.0
62	143251.0
63	110410.5
64	82719.5
65	63286.0
66	49288.0
67	37274.5
68	27793.0
69	20337.5
70	14300.5
71	9961.5
72	7053.5
73	4847.5
74	3196.5
75	2079.0
76	1325.5
77	815.0
78	547.5
79	382.5
80	244.0
81	148.0
82	107.0
83	86.5
84	63.0
85	44.5
86	33.0
87	24.5
88	19.0
89	13.0
90	8.0
91	7.0
92	5.0
93	4.0
94	3.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.3295855861222112E-5
2	0.015808772618993088
3	1.3295855861222112E-5
4	1.063668468897769E-4
5	1.3295855861222112E-5
6	6.647927930611056E-6
7	6.647927930611056E-6
8	0.0
9	0.0
10-11	1.2963459464691558E-4
12-13	6.315531534080503E-4
14-15	2.758890091203588E-4
16-17	0.001964462703495567
18-19	0.0
20-21	0.0
22-23	1.9613508175458724E-4
24-25	0.009206899888180554
26-27	0.01948780565279409
28-29	0.3470281222656274
30-31	0.3231022452949204
32-33	0.31708217747325784
34-35	0.1855206217880258
36-37	0.04509023987099077
38-39	0.03638994296945388
40-41	0.014921059483836315
42-43	0.0139862838955341
44-45	0.017132726616262584
46-47	0.011954266631478498
48-49	0.007006500695716162
50-51	0.00397868282404409
52-53	0.001651183493863263
54-55	1.1237143529714379E-4
56-57	3.1806901834468545E-4
58-59	0.0013170608558482795
60-61	0.0012925404103260793
62-63	3.4985503023255316E-4
64-65	1.3304754214432953E-4
66-67	0.0031308381092076333
68-69	0.005371681847895924
70-71	0.0071504750537801556
72-73	0.011089491359076296
74-75	0.008106575084785796
76-77	0.006237599409958133
78-79	0.004077907915314099
80-81	0.004815079643421845
82-83	0.001556968240978744
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.002590600994287927
92-93	0.015106089752765952
94-95	0.001303207960835004
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	272.0
20-21	965.0
22-23	1990.0
24-25	5159.0
26-27	6396.0
28-29	11600.0
30-31	32461.0
32-33	42664.0
34-35	61731.0
36-37	84795.0
38-39	98048.0
40-41	106431.0
42-43	125108.0
44-45	147929.0
46-47	146378.0
48-49	128694.0
50-51	115869.0
52-53	103541.0
54-55	114468.0
56-57	122666.0
58-59	129221.0
60-61	132406.0
62-63	134545.0
64-65	148499.0
66-67	158011.0
68-69	147916.0
70-71	142903.0
72-73	140166.0
74-75	143737.0
76-77	143746.0
78-79	152357.0
80-81	155063.0
82-83	154636.0
84-85	160467.0
86-87	171270.0
88-89	185289.0
90-91	200140.0
92-93	217627.0
94-95	261835.0
96-97	397471.0
98-99	1433322.0
100-101	8674489.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.7359076801243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.48523776176235	75.82612845714668
2	7.342186466794827	12.44293669241169
3	1.7759190062063852	4.514523268718471
4	0.6513167250222783	2.207596555280347
5	0.2893471044091112	1.2259044763360867
6	0.15231645980657157	0.7744004085799808
7	0.0920861438158625	0.5462102086699702
8	0.0540134809738379	0.36615050698250234
9	0.03409360818032498	0.2600057551725314
>10	0.1201031857664431	1.5802119402495092
>50	0.0027391204149724165	0.15654755410523835
>100	6.14843376248126E-4	0.08417231340934028
>500	2.609347081489129E-5	0.015211862937634723
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.329585586122211E-5
2	0.0	2.659171172244422E-5	0.0	6.647927930611055E-6	1.329585586122211E-5
3	0.0	2.659171172244422E-5	0.0	1.9943783791833166E-5	1.329585586122211E-5
4	0.0	7.31272072367216E-5	0.0	3.988756758366633E-5	1.329585586122211E-5
5	0.0	7.31272072367216E-5	0.0	5.318342344488844E-5	1.329585586122211E-5
6	6.647927930611055E-6	7.31272072367216E-5	0.0	5.318342344488844E-5	1.329585586122211E-5
7	6.647927930611055E-6	7.977513516733266E-5	0.0	5.98313513754995E-5	1.9943783791833166E-5
8	6.647927930611055E-6	1.1301477482038794E-4	0.0	6.647927930611056E-5	1.9943783791833166E-5
9	6.647927930611055E-6	1.794940541264985E-4	0.0	1.19662702750999E-4	3.323963965305528E-5
10-11	1.329585586122211E-5	1.794940541264985E-4	0.0	1.7617009016119298E-4	3.323963965305528E-5
12-13	1.9943783791833166E-5	1.794940541264985E-4	0.0	2.45973333432609E-4	3.65636036183608E-5
14-15	2.659171172244422E-5	2.3267747757138693E-4	0.0	2.692410811897478E-4	4.321153154897186E-5
16-17	3.988756758366634E-5	2.39325405501998E-4	0.0	3.091286487734141E-4	4.653549551427739E-5
18-19	4.653549551427739E-5	2.6591711722444224E-4	0.0	3.7560792807952466E-4	4.985945947958292E-5
20-21	5.318342344488844E-5	2.991567568774975E-4	0.0	4.587070272121628E-4	5.318342344488844E-5
22-23	5.650738741019397E-5	3.4236828842646935E-4	0.0	7.778075678814935E-4	6.980324327141608E-5
24-25	8.309909913263819E-5	3.6231207221830257E-4	0.0	0.001688573694375208	7.977513516733266E-5
26-27	9.307099102855478E-5	3.722839641142191E-4	0.0	0.004500647209023685	7.977513516733266E-5
28-29	9.971891895916584E-5	3.7560792807952466E-4	0.0	0.02211100829721237	8.642306309794372E-5
30-31	1.0969081085508242E-4	4.321153154897186E-4	0.0	0.031231965418010738	8.642306309794372E-5
32-33	1.19662702750999E-4	4.454111713509407E-4	0.0	0.03479193082485296	8.642306309794372E-5
34-35	1.3960648654283216E-4	5.218623425529679E-4	0.0	0.0394454803762807	8.642306309794372E-5
36-37	1.5290234240405428E-4	5.916655858243839E-4	0.0	0.047140456955962994	9.307099102855478E-5
38-39	1.5622630636935982E-4	6.016374777203005E-4	0.0	0.06186561732226649	9.307099102855478E-5
40-41	1.7284612619588744E-4	6.681167570264111E-4	0.0	0.08988330958582677	9.307099102855478E-5
42-43	1.7284612619588744E-4	6.913845047835498E-4	0.0	0.10321240508670194	9.63949549938603E-5
44-45	1.7284612619588744E-4	7.81131531846799E-4	0.0	0.12318278059025756	1.0636684688977688E-4
46-47	1.794940541264985E-4	8.542587390835206E-4	0.0	0.14830862420400204	1.2631063068161005E-4
48-49	1.794940541264985E-4	8.575827030488261E-4	0.0	0.16793995538309647	1.2631063068161005E-4
50-51	1.794940541264985E-4	9.971891895916582E-4	0.0	0.19309571467252873	1.2631063068161005E-4
52-53	1.8946594602241507E-4	0.0010038371175222693	0.0	0.22173166423363583	1.2631063068161005E-4
54-55	1.927899099877206E-4	0.0010171329733834914	0.0	0.3078688664305633	1.2631063068161005E-4
56-57	1.9943783791833168E-4	0.0010570205409671579	0.0	0.3809528621357359	1.3960648654283216E-4
58-59	1.9943783791833168E-4	0.0010570205409671579	0.0	0.4381350142308869	1.5290234240405428E-4
60-61	1.9943783791833168E-4	0.0010836122526896022	0.0	0.46309465964636615	1.5290234240405428E-4
62-63	1.9943783791833168E-4	0.0011068800004467407	0.0	0.47640713532741474	1.5290234240405428E-4
64-65	1.9943783791833168E-4	0.0012431625230242675	0.0	0.4864288866828109	1.5290234240405428E-4
66-67	1.9943783791833168E-4	0.0012631063068161005	0.0	0.4930269551539424	1.5290234240405428E-4
68-69	1.9943783791833168E-4	0.0012764021626773226	0.0	0.4993790502916413	1.5290234240405428E-4
70-71	1.9943783791833168E-4	0.0012896980185385448	0.0	0.5049101263299096	1.5955027033466533E-4
72-73	1.9943783791833168E-4	0.0012996699104344614	0.0	0.5095005205659966	2.2935351360608142E-4
74-75	1.9943783791833168E-4	0.0013428814419834331	0.0	0.5135291648919469	2.3600144153669247E-4
76-77	2.0608576584894272E-4	0.0013761210816364885	0.0	0.5171190459744769	2.4264936946730354E-4
78-79	2.1273369377955377E-4	0.0013960648654283217	0.0	0.5203532629127192	2.4929729739791456E-4
80-81	2.1273369377955377E-4	0.0014126846852548494	0.0	0.5217592996700434	2.6591711722444224E-4
82-83	2.1273369377955377E-4	0.0014359524330119881	0.0	0.5223011057963882	2.858609010162754E-4
84-85	2.1273369377955377E-4	0.0014658681086997377	0.0	0.522713277328086	2.858609010162754E-4
86-87	2.1273369377955377E-4	0.0014957837843874876	0.0	0.5228661796704901	2.858609010162754E-4
88-89	2.1273369377955377E-4	0.0015323473880058482	0.0	0.5229127151660045	2.858609010162754E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18980	0.0	50.11594	1
GTATCAA	35725	0.0	38.27138	1
TCAACGC	44195	0.0	30.263418	4
ATCAACG	44275	0.0	30.141665	3
TATCAAC	46030	0.0	29.308773	2
CAACGCA	45660	0.0	29.27382	5
AACGCAG	46805	0.0	28.612112	6
ACGCAGA	54295	0.0	24.641735	7
CGCAGAG	55150	0.0	24.259708	8
GCAGAGT	63320	0.0	21.003033	9
GTGGTAT	9270	0.0	20.777283	1
GTACATG	43335	0.0	20.098032	1
TGGTATC	9075	0.0	19.920527	2
TACATGG	42930	0.0	19.677631	2
GAGTACT	38755	0.0	18.994373	12-13
ACATGGG	43820	0.0	18.908861	3
AGAGTAC	58210	0.0	18.737038	10-11
CAGAGTA	61975	0.0	18.154148	10-11
GTACTTT	43065	0.0	17.049517	14-15
AGTACTT	42550	0.0	16.903257	12-13
>>END_MODULE