FastQCFastQC Report
Fri 27 May 2016
SRR2139089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8927338
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116400.048.67751
GTATCAA210150.036.029741
CTTATAC123150.029.1290111
TCAACGC262050.028.2932074
ATCAACG263000.028.0946273
TATCAAC271400.027.5715522
CAACGCA270200.027.4240175
AACGCAG278450.026.6419836
TATACAC161750.024.8123873
ACGCAGA320900.023.1439977
CGCAGAG322100.023.0184278
TTATACA166650.022.590452
GCAGAGT371050.019.7882149
GTGGTAT61800.019.758861
GTACATG251050.019.2875421
ACATCTC195050.018.7135878
TACACAT208250.018.6430665
TACATGG254800.018.5393662
GAGTACT221000.018.41924312-13
AGAGTAC337850.018.388710-11
Produced by FastQC (version 0.11.5)