##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139089_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8927338
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56617975033543	34.0	31.0	34.0	31.0	34.0
2	32.6981104557708	34.0	31.0	34.0	31.0	34.0
3	32.682813510589604	34.0	31.0	34.0	31.0	34.0
4	36.20755761683942	37.0	35.0	37.0	35.0	37.0
5	36.1643638898852	37.0	35.0	37.0	35.0	37.0
6	36.20632959119504	37.0	37.0	37.0	35.0	37.0
7	36.165194708657836	37.0	37.0	37.0	35.0	37.0
8	36.14383436585464	37.0	36.0	37.0	35.0	37.0
9	37.87221319501961	39.0	38.0	39.0	35.0	39.0
10-11	37.80353891608003	39.0	38.0	39.0	35.0	39.0
12-13	37.77030666924452	39.0	38.0	39.0	35.0	39.0
14-15	39.115715289372936	40.0	38.5	41.0	36.0	41.0
16-17	39.08320699854761	40.0	38.0	41.0	36.0	41.0
18-19	39.04752984596304	40.0	38.5	41.0	36.0	41.0
20-21	39.035967554277896	40.0	39.0	41.0	35.5	41.0
22-23	38.91920449939184	40.0	38.0	41.0	35.0	41.0
24-25	38.780422991445505	40.0	38.0	41.0	35.0	41.0
26-27	38.72744382385413	40.0	38.0	41.0	34.5	41.0
28-29	38.66721993175328	40.0	38.0	41.0	35.0	41.0
30-31	38.60385440103167	40.0	38.0	41.0	34.5	41.0
32-33	38.49065628798452	40.0	38.0	41.0	34.0	41.0
34-35	38.29154690435892	40.0	38.0	41.0	34.0	41.0
36-37	38.42538134119597	40.0	38.0	41.0	34.0	41.0
38-39	38.393567133711066	40.0	38.0	41.0	34.0	41.0
40-41	38.37503162975469	40.0	38.0	41.0	34.0	41.0
42-43	38.4171083623193	40.0	38.0	41.0	34.0	41.0
44-45	38.35973560764826	40.0	38.0	41.0	34.0	41.0
46-47	38.29882995648907	40.0	38.0	41.0	33.0	41.0
48-49	38.14147518310292	40.0	38.0	41.0	33.0	41.0
50-51	38.131567793550104	40.0	37.0	41.0	33.0	41.0
52-53	38.039985729822845	40.0	37.0	41.0	33.0	41.0
54-55	37.892486482865124	40.0	37.0	41.0	33.0	41.0
56-57	37.71229948553885	40.0	36.5	41.0	33.0	41.0
58-59	37.53960781033172	39.0	36.0	41.0	32.5	41.0
60-61	37.35632416195442	39.0	35.5	41.0	32.0	41.0
62-63	37.14682253677069	39.0	35.0	40.5	32.0	41.0
64-65	36.858166092219776	38.0	35.0	40.0	31.5	41.0
66-67	36.50933068373192	38.0	35.0	40.0	31.0	41.0
68-69	36.181194080951705	37.0	35.0	40.0	31.0	41.0
70-71	35.886370346361886	37.0	34.5	39.0	31.0	41.0
72-73	35.56807907129947	36.0	34.0	39.0	30.0	40.5
74-75	35.20620968166745	36.0	34.0	39.0	30.0	40.0
76-77	34.254756931678074	35.0	33.0	37.0	29.0	39.0
78-79	34.53764814831597	35.0	34.0	37.0	30.0	39.0
80-81	34.347325054967676	35.0	34.0	37.0	30.0	39.0
82-83	34.07836587385427	35.0	34.0	36.0	29.0	38.5
84-85	33.83700720276945	35.0	33.0	36.0	29.0	37.0
86-87	33.60167060771074	35.0	33.0	35.5	29.0	37.0
88-89	33.370245400924794	35.0	33.0	35.0	29.0	36.5
90-91	33.18955160883864	35.0	33.0	35.0	29.0	36.0
92-93	33.10286102033621	35.0	33.0	35.0	29.0	36.0
94-95	33.02658212808409	34.5	33.0	35.0	29.0	36.0
96-97	33.007167159687086	34.0	33.0	35.0	29.0	35.0
98-99	33.12884960453847	34.0	33.0	35.0	29.0	35.0
100-101	31.275249957539327	32.5	29.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	11.0
20	47.0
21	256.0
22	1225.0
23	4131.0
24	10860.0
25	22459.0
26	39212.0
27	62402.0
28	89705.0
29	119906.0
30	154421.0
31	193490.0
32	245625.0
33	321269.0
34	443514.0
35	639828.0
36	1057834.0
37	1941207.0
38	2352745.0
39	1156430.0
40	70758.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.2873681465557	21.631313081528464	15.509273987223063	24.572044784692768
2	13.600354469690265	21.52225910569224	41.83672211884145	23.04066430577604
3	16.96065881243856	26.619452880456162	31.40350043954882	25.016387867556457
4	11.9923632201949	18.91595050386325	38.57912388741843	30.512562388523413
5	12.881984054369635	37.977253236575834	35.898167157593264	13.242595551461264
6	29.467290130000706	38.41901996295423	18.601943513720105	13.511746393324952
7	25.90005573891248	33.00367545256502	23.021190196045048	18.07507861247745
8	24.00264222100698	37.377648297846456	21.144354565717126	17.475354915429435
9	24.937926624935674	17.095432031362538	21.48509443688589	36.48154690681589
10-11	23.84130796568604	27.08892547815814	28.96383677581503	20.105929780340794
12-13	24.683866350504545	24.500266122596294	29.132613961268028	21.68325356563113
14-15	21.84674504864681	26.024499885575796	26.663231140141143	25.46552392563625
16-17	21.431176238151686	29.336948141725806	27.33371475330781	21.8981608668147
18-19	21.462792155959594	28.29328854805318	29.162528628354835	21.081390667632387
20-21	22.112985369804363	27.39764996076547	29.101470412794324	21.387894256635846
22-23	21.883333735704326	27.602909461541913	28.92558599453197	21.58817080822179
24-25	21.78340819134964	27.868558064270317	28.98109050647948	21.366943237900568
26-27	21.758449291358797	28.034067919459925	28.921003168887164	21.28647962029411
28-29	21.563018630291634	28.00078051959919	28.956269971556498	21.47993087855268
30-31	22.05747580443612	27.707914709616627	28.917515514770752	21.3170939711765
32-33	21.693054209652253	27.87443418682869	28.939243966884	21.49326763663506
34-35	21.773793903473837	27.658703754687213	29.05065212512345	21.5168502167155
36-37	21.594979147347498	27.927852175374905	28.971485546309445	21.505683130968155
38-39	21.608902578908996	27.623035659044888	29.093627920219078	21.67443384182704
40-41	21.764013855169527	27.505280290304473	29.05896184031383	21.671744014212173
42-43	21.877538938459587	27.57638748569465	28.88519264640459	21.660880929441177
44-45	21.71892075024497	27.887737184431234	28.630234425594352	21.763107639729444
46-47	22.020603826333975	27.58030877749495	28.243371392080878	22.155716004090195
48-49	21.964527488699986	27.76717548734849	28.137819606691288	22.130477417260234
50-51	21.70997671499766	27.907133232700374	28.25297173259182	22.12991831971015
52-53	21.858653094893015	27.860995818484064	28.33203356830761	21.94831751831531
54-55	21.883575627273537	27.878924710348084	28.077967517233336	22.159532145145043
56-57	22.111693544468945	28.050076340084285	27.816843329827417	22.021386785619356
58-59	21.73968572731733	28.356918706614465	28.18683182036679	21.716563745701418
60-61	22.06703265793316	28.544125835154382	27.680768094875695	21.708073412036764
62-63	21.824143515280873	28.73119428726566	27.617976234390103	21.82668596306337
64-65	21.82988770354346	28.583160015346365	27.703592212663498	21.88336006844668
66-67	21.95942707128539	28.432937102984557	27.583323318543947	22.02431250718611
68-69	21.924256406199913	28.226547253185423	27.769371073030623	22.07982526758404
70-71	21.916851953394378	28.021750853616247	27.827809563007662	22.233587629981717
72-73	21.990212674245843	27.966460476060245	27.8005056349166	22.242821214777305
74-75	21.951819149414604	28.07449674764949	27.71215914371805	22.261524959217855
76-77	22.081602969425767	28.032922217982847	27.659262859461737	22.226211953129653
78-79	21.998734097509637	27.998870631445744	27.647759459280984	22.354635811763632
80-81	21.971378237393612	28.002995750700215	27.69909907161669	22.326526940289483
82-83	22.00146922811161	27.95797320911894	27.812974557461196	22.227583005308247
84-85	22.160218254866862	28.01198810464889	27.68972029229248	22.13807334819177
86-87	22.032102863400507	27.95542623200966	27.797782243386067	22.21468866120376
88-89	22.015057703545448	27.97398967985319	27.785509207171522	22.22544340942984
90-91	22.082195715378454	27.978842884067	27.803688642180052	22.135272758374498
92-93	22.15392042371172	27.84956201568225	27.86164631202719	22.13487124857884
94-95	21.979864859401335	27.911096000390273	27.937994239379194	22.171044900829195
96-97	21.878153200935547	27.92393088700998	28.038755911847495	22.159160000206978
98-99	22.002020706720508	28.487733001590733	28.04401877608734	21.466227515601418
100-101	20.655658605360742	31.927184054803813	25.934224073733876	21.48293326610157
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1728.0
1	1594.5
2	2232.0
3	3190.0
4	4350.5
5	6614.5
6	8477.5
7	10318.0
8	11894.5
9	12189.5
10	11408.5
11	10593.0
12	11772.5
13	16001.0
14	26430.5
15	40741.0
16	51966.0
17	57614.5
18	57969.5
19	54736.0
20	49823.5
21	45372.0
22	43758.0
23	47192.5
24	55958.5
25	69268.0
26	85625.0
27	103512.5
28	123401.5
29	144499.0
30	164648.5
31	184624.5
32	206345.0
33	227334.0
34	246827.0
35	265803.5
36	286272.0
37	303527.0
38	313558.0
39	321095.0
40	329708.0
41	338998.5
42	345579.5
43	351369.0
44	359079.5
45	369508.0
46	383452.5
47	391636.5
48	382424.5
49	368840.5
50	357897.5
51	342481.0
52	323924.0
53	304100.0
54	284259.0
55	262771.0
56	240639.0
57	217161.5
58	191940.0
59	166250.5
60	139924.5
61	114599.5
62	90632.0
63	69276.0
64	51817.0
65	38787.5
66	29413.0
67	22426.0
68	16985.0
69	12115.0
70	8267.5
71	5749.0
72	3945.0
73	2628.0
74	1698.0
75	1065.0
76	696.0
77	462.5
78	303.5
79	207.0
80	137.0
81	93.0
82	67.0
83	40.0
84	23.5
85	16.0
86	10.5
87	7.5
88	4.5
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.2403094853135392E-5
2	0.0148756549824819
3	2.2403094853135392E-5
4	1.6802321139851542E-4
5	2.2403094853135392E-5
6	2.2403094853135392E-5
7	2.2403094853135392E-5
8	0.0
9	0.0
10-11	1.0081392683910926E-4
12-13	6.888951667339133E-4
14-15	3.472479702235986E-4
16-17	0.0019098638362297922
18-19	0.0
20-21	0.0
22-23	3.360860084425926E-4
24-25	0.009423601651248078
26-27	0.019806525998111037
28-29	0.3455903503395963
30-31	0.32168574376665104
32-33	0.31535602584798766
34-35	0.1852113820895069
36-37	0.04616607625359514
38-39	0.03821481239879389
40-41	0.015517475380211145
42-43	0.013832979232958122
44-45	0.017391734412001907
46-47	0.011913484066598852
48-49	0.007086645095339475
50-51	0.004243715819180011
52-53	0.001718467988208889
54-55	7.926099487181363E-5
56-57	2.3369986492147808E-4
58-59	0.0011548439619542246
60-61	0.001329855969071063
62-63	3.804146798981655E-4
64-65	1.1546322659805757E-4
66-67	0.003295185013264582
68-69	0.005486102970135315
70-71	0.0071035231209015635
72-73	0.011037726923670952
74-75	0.008108073119829825
76-77	0.006119765464123564
78-79	0.0038629662239386625
80-81	0.004533247131026518
82-83	0.0014949565958307593
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.002570379297755695
92-93	0.015224667296241143
94-95	0.0011821529890383682
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	150.0
20-21	622.0
22-23	1277.0
24-25	3148.0
26-27	3896.0
28-29	6572.0
30-31	18454.0
32-33	24126.0
34-35	35180.0
36-37	48986.0
38-39	57460.0
40-41	60879.0
42-43	72743.0
44-45	85816.0
46-47	85269.0
48-49	75360.0
50-51	68621.0
52-53	61056.0
54-55	68279.0
56-57	76137.0
58-59	81780.0
60-61	84526.0
62-63	89142.0
64-65	96468.0
66-67	106180.0
68-69	90612.0
70-71	84533.0
72-73	81178.0
74-75	83225.0
76-77	83073.0
78-79	88785.0
80-81	90916.0
82-83	90256.0
84-85	92714.0
86-87	99400.0
88-89	107907.0
90-91	116315.0
92-93	125786.0
94-95	149987.0
96-97	227146.0
98-99	836026.0
100-101	5167352.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.81177653356507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.9016211581955	74.51002805982341
2	7.517507076103949	12.601112463038366
3	1.9834921776039607	4.9872000943625245
4	0.750487815434328	2.5159886831338127
5	0.32722003015432427	1.3712446022300324
6	0.17865966714707268	0.8984270459094942
7	0.10035710734617422	0.5887775218116789
8	0.06398154514372846	0.42899255710866996
9	0.0410996991440627	0.3100174920233045
>10	0.13391573005577714	1.648570024656796
>50	0.001262021195537908	0.0691105658204178
>100	3.738074144982096E-4	0.06040646241204199
>500	2.216506113096297E-5	0.010124427669467872
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2403094853135392E-5
2	0.0	2.2403094853135392E-5	0.0	0.0	2.2403094853135392E-5
3	0.0	2.2403094853135392E-5	0.0	0.0	2.2403094853135392E-5
4	0.0	5.600773713283848E-5	0.0	0.0	2.2403094853135392E-5
5	0.0	5.600773713283848E-5	0.0	0.0	2.2403094853135392E-5
6	0.0	6.720928455940618E-5	0.0	1.1201547426567696E-5	3.360464227970309E-5
7	0.0	6.720928455940618E-5	0.0	1.1201547426567696E-5	4.4806189706270784E-5
8	0.0	6.720928455940618E-5	0.0	2.2403094853135392E-5	6.720928455940618E-5
9	0.0	1.3441856911881235E-4	0.0	5.600773713283848E-5	7.841083198597387E-5
10-11	0.0	1.3441856911881235E-4	0.0	7.841083198597388E-5	7.841083198597387E-5
12-13	0.0	1.3441856911881235E-4	0.0	1.0081392683910926E-4	8.961237941254157E-5
14-15	0.0	1.4562011654538004E-4	0.0	1.3441856911881235E-4	1.2321702169224464E-4
16-17	0.0	1.4562011654538004E-4	0.0	1.4562011654538004E-4	1.5682166397194773E-4
18-19	0.0	1.624224376852316E-4	0.0	1.7922475882508314E-4	1.8482553253836698E-4
20-21	0.0	1.7922475882508314E-4	0.0	2.57635590811057E-4	2.2403094853135392E-4
22-23	0.0	2.0722862739150236E-4	0.0	4.4806189706270784E-4	2.5203481709777317E-4
24-25	0.0	2.5203481709777317E-4	0.0	0.0010977516478036342	3.0244178051732777E-4
26-27	0.0	2.57635590811057E-4	0.0	0.0032652510748444833	3.0244178051732777E-4
28-29	0.0	2.57635590811057E-4	0.0	0.01847135170641013	3.0244178051732777E-4
30-31	0.0	3.248448753704632E-4	0.0	0.026435651926699763	3.0244178051732777E-4
32-33	0.0	3.248448753704632E-4	0.0	0.029392860447313634	3.5844951765016627E-4
34-35	0.0	4.0885648106972093E-4	0.0	0.03329659972547248	3.8085261250330165E-4
36-37	0.0	4.928680867689787E-4	0.0	0.04040398156762968	3.8085261250330165E-4
38-39	0.0	5.15271181622114E-4	0.0	0.053571400567560004	3.8085261250330165E-4
40-41	0.0	5.600773713283848E-4	0.0	0.08055592831816158	3.9205415992986934E-4
42-43	0.0	5.992827873213718E-4	0.0	0.09264239799142812	4.0325570735643703E-4
44-45	0.0	7.281005827269001E-4	0.0	0.11123696671953051	4.0325570735643703E-4
46-47	0.0	8.233137358527255E-4	0.0	0.1353707006500706	4.088564810697209E-4
48-49	0.0	8.401160569925772E-4	0.0	0.15399887402045268	4.144572547830047E-4
50-51	0.0	9.913369472512412E-4	0.0	0.17737090272598618	4.312595759228563E-4
52-53	0.0	0.0010081392683910927	0.0	0.20442824053486044	4.648642182025594E-4
54-55	0.0	0.0010137400421043764	0.0	0.28300149495851956	4.928680867689787E-4
56-57	0.0	0.0010417439106707956	0.0	0.350748453794401	5.208719553353978E-4
58-59	0.0	0.0010585462318106471	0.0	0.40426384662482817	5.376742764752494E-4
60-61	0.0	0.0010809493266637827	0.0	0.4276526776515015	5.488758239018171E-4
62-63	0.0	0.0011145539689434857	0.0	0.44066887576117314	5.54476597615101E-4
64-65	0.0	0.0013553872386146912	0.0	0.4504310243434269	5.600773713283848E-4
66-67	0.0	0.001360988012327975	0.0	0.4564014491217875	5.656781450416686E-4
68-69	1.1201547426567696E-5	0.0014001934283209621	0.0	0.46176138956540014	5.768796924682364E-4
70-71	1.1201547426567696E-5	0.0014225965231740973	0.0	0.46717733774614556	5.992827873213718E-4
72-73	1.1201547426567696E-5	0.0014393988443139488	0.0	0.4717083636801922	6.720928455940618E-4
74-75	1.1201547426567696E-5	0.0014618019391670844	0.0	0.4759817540234278	6.888951667339133E-4
76-77	1.1201547426567696E-5	0.0015010073551600714	0.0	0.4794206290833841	6.944959404471972E-4
78-79	1.1201547426567696E-5	0.001512208902586639	0.0	0.48269148093194186	7.000967141604811E-4
80-81	1.1201547426567696E-5	0.001517809676299923	0.0	0.48405806971798315	7.393021301534679E-4
82-83	1.1201547426567696E-5	0.0015234104500132066	0.0	0.48453413548361224	7.953098672863064E-4
84-85	1.1201547426567696E-5	0.0015290112237264905	0.0	0.48486458113269604	7.953098672863064E-4
86-87	1.1201547426567696E-5	0.0015570150922929098	0.0	0.4850550074389477	7.953098672863064E-4
88-89	1.1201547426567696E-5	0.0016466274717054513	0.0	0.48512781749722034	8.065114147128741E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11640	0.0	48.6775	1
GTATCAA	21015	0.0	36.02974	1
CTTATAC	12315	0.0	29.129011	1
TCAACGC	26205	0.0	28.293207	4
ATCAACG	26300	0.0	28.094627	3
TATCAAC	27140	0.0	27.571552	2
CAACGCA	27020	0.0	27.424017	5
AACGCAG	27845	0.0	26.641983	6
TATACAC	16175	0.0	24.812387	3
ACGCAGA	32090	0.0	23.143997	7
CGCAGAG	32210	0.0	23.018427	8
TTATACA	16665	0.0	22.59045	2
GCAGAGT	37105	0.0	19.788214	9
GTGGTAT	6180	0.0	19.75886	1
GTACATG	25105	0.0	19.287542	1
ACATCTC	19505	0.0	18.713587	8
TACACAT	20825	0.0	18.643066	5
TACATGG	25480	0.0	18.539366	2
GAGTACT	22100	0.0	18.419243	12-13
AGAGTAC	33785	0.0	18.3887	10-11
>>END_MODULE