FastQCFastQC Report
Fri 27 May 2016
SRR2139088_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139088_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13072417
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA170150.050.3592831
GTATCAA320700.037.1533741
TCAACGC397650.029.4992774
ATCAACG397000.029.4721953
CAACGCA403300.029.0543025
TATCAAC413150.028.5507052
AACGCAG417350.028.1892956
ACGCAGA480200.024.4909217
CGCAGAG484900.024.1920018
GCAGAGT561400.020.789229
GTACATG361700.019.7887171
GTGGTAT87750.019.3839021
GAGTACT353400.019.19044512-13
TACATGG369050.019.1751292
AGAGTAC517100.019.11256210-11
ACATGGG369150.018.4978083
CAGAGTA551650.018.1859910-11
TGGTATC89850.017.460042
AGTACTT386600.017.41562812-13
GTACTTT394350.017.18192114-15
Produced by FastQC (version 0.11.5)