##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139088_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13072417
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55348081383879	34.0	31.0	34.0	31.0	34.0
2	32.69612612571952	34.0	31.0	34.0	31.0	34.0
3	32.684006943780936	34.0	31.0	34.0	31.0	34.0
4	36.214553437210576	37.0	35.0	37.0	35.0	37.0
5	36.17089762359937	37.0	35.0	37.0	35.0	37.0
6	36.214306275572454	37.0	37.0	37.0	35.0	37.0
7	36.17371577115387	37.0	37.0	37.0	35.0	37.0
8	36.14976052248027	37.0	36.0	37.0	35.0	37.0
9	37.88090503844851	39.0	38.0	39.0	35.0	39.0
10-11	37.80826124197232	39.0	38.0	39.0	35.0	39.0
12-13	37.780714385105675	39.0	38.0	39.0	35.0	39.0
14-15	39.11968364381277	40.0	38.5	41.0	36.0	41.0
16-17	39.090304302563176	40.0	38.0	41.0	36.0	41.0
18-19	39.05135538439448	40.0	38.5	41.0	36.0	41.0
20-21	39.03759997047475	40.0	39.0	41.0	35.5	41.0
22-23	38.919965259093914	40.0	38.0	41.0	35.0	41.0
24-25	38.78458075407163	40.0	38.0	41.0	35.0	41.0
26-27	38.73282124712846	40.0	38.0	41.0	34.5	41.0
28-29	38.6761976299819	40.0	38.0	41.0	35.0	41.0
30-31	38.6071784058462	40.0	38.0	41.0	35.0	41.0
32-33	38.49234029381684	40.0	38.0	41.0	34.0	41.0
34-35	38.30078049079931	40.0	38.0	41.0	34.0	41.0
36-37	38.437456120684075	40.0	38.0	41.0	34.0	41.0
38-39	38.42024960175038	40.0	38.0	41.0	34.0	41.0
40-41	38.38604478188738	40.0	38.0	41.0	34.0	41.0
42-43	38.41617276967297	40.0	38.0	41.0	34.0	41.0
44-45	38.34948895915696	40.0	38.0	41.0	34.0	41.0
46-47	38.28940560332809	40.0	38.0	41.0	33.5	41.0
48-49	38.12971217326584	40.0	38.0	41.0	33.0	41.0
50-51	38.12555861477526	40.0	37.0	41.0	33.0	41.0
52-53	38.029394906812584	40.0	37.0	41.0	33.0	41.0
54-55	37.880124240624625	40.0	37.0	41.0	33.0	41.0
56-57	37.71425008641809	40.0	36.5	41.0	33.0	41.0
58-59	37.550089206220704	39.0	36.0	41.0	33.0	41.0
60-61	37.37184295838743	39.0	36.0	41.0	32.0	41.0
62-63	37.16039715744509	39.0	35.0	40.5	32.0	41.0
64-65	36.86713884143087	38.0	35.0	40.0	31.5	41.0
66-67	36.51359620112554	38.0	35.0	40.0	31.0	41.0
68-69	36.17938166073441	37.0	35.0	40.0	31.0	41.0
70-71	35.879893994865796	37.0	34.5	39.0	31.0	41.0
72-73	35.554190880643915	36.0	34.0	39.0	30.0	40.5
74-75	35.186402839519324	36.0	34.0	39.0	30.0	40.0
76-77	34.22486481999633	35.0	33.0	37.0	29.0	39.0
78-79	34.513548688925	35.0	34.0	37.0	29.5	39.0
80-81	34.3153756783342	35.0	34.0	37.0	29.5	39.0
82-83	34.035940070326234	35.0	33.5	36.0	29.0	38.5
84-85	33.79321183004285	35.0	33.0	36.0	29.0	37.0
86-87	33.55546807573822	35.0	33.0	35.5	29.0	37.0
88-89	33.31777453273152	35.0	33.0	35.0	29.0	36.5
90-91	33.12895476229582	35.0	33.0	35.0	29.0	36.0
92-93	33.042916128254504	34.5	33.0	35.0	29.0	36.0
94-95	32.960987387527496	34.0	32.5	35.0	29.0	35.5
96-97	32.938417810845124	34.0	32.0	35.0	29.0	35.0
98-99	33.064351791049006	34.0	32.0	35.0	29.0	35.0
100-101	31.207703031041426	32.5	28.5	34.5	24.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	10.0
20	65.0
21	334.0
22	1656.0
23	5480.0
24	14459.0
25	30456.0
26	54991.0
27	86740.0
28	127231.0
29	172529.0
30	225288.0
31	285665.0
32	364264.0
33	476762.0
34	663967.0
35	964623.0
36	1605279.0
37	2918469.0
38	3425827.0
39	1563771.0
40	84548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.271966079417446	21.73070978381427	15.77517761252567	24.22214652424261
2	13.561230604254257	21.923183329161052	41.47366811951776	23.041917947066928
3	16.72798534502074	26.890375360577924	31.604889899090583	24.776749395310752
4	11.916281631529023	19.100991401731893	39.195633548545025	29.787093418194054
5	12.7775376198602	38.129475214874184	35.98887642583617	13.104110739429442
6	29.208691858590498	38.880858834292084	18.79317344298304	13.117275864134385
7	25.72503615819477	33.23571302843231	23.527554238822095	17.51169657455083
8	24.09430482519032	37.08331825705989	21.280907731141074	17.54146918660872
9	25.21524519910893	16.913796431065503	21.67722311795898	36.193735251866585
10-11	23.707307904719404	27.189832287484787	29.24523917739815	19.85762063039766
12-13	24.59820983076518	24.776215458602493	29.363699588738495	21.261875121893826
14-15	21.78202522348743	26.184681985402886	27.073296028469795	24.959996762639886
16-17	21.162350120637875	29.322855866427787	27.77687424744763	21.737919765486705
18-19	21.191482799240568	28.376232949117213	29.670285915756818	20.761998335885398
20-21	21.987470669283855	27.478026203547156	29.597001907964376	20.937501219204613
22-23	21.748165195765623	27.443267021461914	29.44500935007868	21.36355843269378
24-25	21.57635806136342	27.61122761659223	29.48190650703626	21.330507815008087
26-27	21.548412511104182	27.69340992036763	29.430579649309784	21.3275979192184
28-29	21.42268989667107	27.723814438214735	29.434579127002376	21.418916538111823
30-31	21.70689583499253	27.618811355565683	29.366434678524612	21.307858130917175
32-33	21.57194500838425	27.673441689150728	29.32756193939397	21.427051363071055
34-35	21.576215065454832	27.736748076735175	29.35995981746832	21.327077040341674
36-37	21.652996505134272	27.74312291940265	29.208911222765018	21.39496935269806
38-39	21.566844010737892	27.803718740627588	29.220705260688785	21.408731987945735
40-41	21.702407659596414	27.67409464048863	29.140962072299477	21.48253562761548
42-43	21.684232034158835	27.823096108086958	28.888920670950313	21.603751186803898
44-45	21.760014026314998	27.967167073416288	28.640792613962518	21.632026286306193
46-47	21.81688046382003	27.912092754054026	28.382198626593247	21.8888281555327
48-49	21.814261840908806	28.065232201356523	28.236574616379272	21.883931341355396
50-51	21.761467297942595	28.124330892642053	28.18971275066704	21.924489058748314
52-53	21.911394587764292	28.073371945628228	28.09916906531193	21.91606440129555
54-55	21.90229247633496	28.007843173978582	28.075756796634998	22.014107553051456
56-57	21.943149956298498	28.12400602465994	28.003480250127634	21.92936376891393
58-59	21.814940332370018	28.23269059688686	28.12741447288768	21.824954597855438
60-61	21.898499707345803	28.133433712247218	28.078646543057516	21.88942003734946
62-63	21.974950437393527	28.231769088077098	28.00353924611511	21.789741228414265
64-65	21.76109450985971	28.19227513102441	28.004545877673515	22.042084481442366
66-67	21.838985499999282	28.195091552897384	27.861340029260965	22.104582917842368
68-69	21.784608325733217	28.26523604666043	27.942113545929796	22.008042081676553
70-71	21.783586006421693	28.122613827591454	27.97888622240057	22.11491394358629
72-73	21.871425294896856	28.10549045899168	27.777414218779423	22.245670027332036
74-75	21.78410039800924	28.167448696050922	27.75659501997005	22.291855885969785
76-77	21.890544175631614	27.94515015513313	27.87933777127838	22.28496789795687
78-79	21.842694763014254	27.88632741423073	27.87892954344117	22.39204827931384
80-81	22.018610395678213	27.933810858909773	27.713207370291208	22.334371375120806
82-83	22.033148551136673	28.093757499129225	27.789111515675348	22.083982434058758
84-85	21.99002837197065	27.91317291291579	27.723574592368195	22.373224122745363
86-87	21.860980671721194	28.08790173219034	27.79054359671932	22.260573999369146
88-89	22.107595317901303	27.821241893846988	27.7798013772361	22.291361411015608
90-91	22.162733952422055	27.98028140057291	27.8040348605059	22.05294978649914
92-93	21.998063475384146	27.841852953477215	27.904355763716893	22.255727807421742
94-95	21.986945618056914	27.74764398904078	27.979696047320225	22.28571434558208
96-97	21.89560568591855	27.979721030631044	28.039795282169493	22.08487800128091
98-99	22.097600598965748	28.380344155093905	28.142536502871735	21.379518743068612
100-101	20.536157778123286	31.98512570070599	26.006510197050424	21.4722063241203
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2984.0
1	2667.0
2	3575.0
3	5046.5
4	6699.5
5	10106.5
6	13024.0
7	16196.5
8	18919.5
9	19330.0
10	18102.5
11	16788.5
12	18400.5
13	24642.0
14	40398.0
15	62465.5
16	79734.0
17	88356.5
18	89189.5
19	84706.0
20	77822.5
21	70769.0
22	67225.0
23	71572.0
24	82907.0
25	100643.0
26	124414.0
27	151101.0
28	180800.0
29	212496.5
30	243638.5
31	275433.0
32	308013.5
33	341159.5
34	371338.0
35	397049.0
36	427496.0
37	454513.5
38	470926.5
39	482372.0
40	493853.0
41	508058.5
42	515338.5
43	520194.5
44	525848.0
45	527057.5
46	523593.5
47	518446.0
48	515398.5
49	511322.0
50	500843.5
51	482003.5
52	456635.5
53	430740.5
54	405500.5
55	374377.0
56	340509.0
57	306947.0
58	271343.5
59	232996.5
60	194492.0
61	158829.0
62	125697.5
63	96128.5
64	71776.0
65	54052.5
66	41452.0
67	31638.0
68	23922.0
69	17104.0
70	11871.5
71	8215.0
72	5560.5
73	3678.5
74	2321.5
75	1463.5
76	960.5
77	598.0
78	381.0
79	255.5
80	180.5
81	137.5
82	108.0
83	67.5
84	36.5
85	24.0
86	16.5
87	11.5
88	9.0
89	9.0
90	8.5
91	7.5
92	6.0
93	3.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.015490631916041234
3	0.0
4	1.3004481114701282E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.3386965853368968E-4
12-13	5.928513449349114E-4
14-15	2.868635540007636E-4
16-17	0.0018129776612848258
18-19	0.0
20-21	0.0
22-23	1.9891303208566664E-4
24-25	0.009052450956835649
26-27	0.019428184439608833
28-29	0.34460860407571964
30-31	0.3199345648850818
32-33	0.3138025251544814
34-35	0.18355711297872124
36-37	0.04410067856568953
38-39	0.03571279416294911
40-41	0.014323222830771183
42-43	0.013035512257222447
44-45	0.016405585162072005
46-47	0.011026295749246627
48-49	0.006472363715734595
50-51	0.0034997058940274355
52-53	0.0012672108733934142
54-55	6.215397844301134E-5
56-57	1.7540710527408238E-4
58-59	0.0010148921312844248
60-61	8.850905938924007E-4
62-63	1.6237399671030283E-4
64-65	3.4509042016052744E-5
66-67	0.0032608973806493033
68-69	0.005371499090216112
70-71	0.007120850134205091
72-73	0.010845583817776911
74-75	0.007899795842351547
76-77	0.006013512749336652
78-79	0.003761566526465782
80-81	0.004583538470993684
82-83	0.0016150335517265233
84-85	0.0
86-87	0.0
88-89	9.922306356765257E-6
90-91	0.002465243097168086
92-93	0.01491899172249998
94-95	0.0012572078607759633
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	226.0
20-21	785.0
22-23	1770.0
24-25	4813.0
26-27	5931.0
28-29	9491.0
30-31	24794.0
32-33	32823.0
34-35	48197.0
36-37	65754.0
38-39	77612.0
40-41	84298.0
42-43	102237.0
44-45	121624.0
46-47	120276.0
48-49	104000.0
50-51	93407.0
52-53	84406.0
54-55	92614.0
56-57	98719.0
58-59	65405.0
60-61	105832.0
62-63	107915.0
64-65	117893.0
66-67	124660.0
68-69	119656.0
70-71	117955.0
72-73	115932.0
74-75	118547.0
76-77	119684.0
78-79	127587.0
80-81	130232.0
82-83	129726.0
84-85	134753.0
86-87	146069.0
88-89	157344.0
90-91	171810.0
92-93	188597.0
94-95	225113.0
96-97	344069.0
98-99	1295931.0
100-101	7733930.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.7548875061614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.2197523666662	73.00615682924875
2	7.855628833712541	13.001833608480784
3	2.15782078934386	5.357106500418226
4	0.811017444970033	2.6846262969611807
5	0.38007036652105686	1.5726340212937795
6	0.19592184989696249	0.97280943889333
7	0.11780844109209462	0.6824457002896771
8	0.07184502458389272	0.47564215418539524
9	0.04894865833078897	0.3645666642367774
>10	0.13846160554152123	1.685782434257269
>50	0.0023269453472781884	0.1225075155744982
>100	3.8180566183629273E-4	0.06394804823854175
>500	1.586833193285572E-5	0.00994078792178525
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.649694773353696E-6	0.0	0.0	0.0
3	0.0	7.649694773353696E-6	0.0	0.0	0.0
4	0.0	3.059877909341478E-5	0.0	0.0	7.649694773353696E-6
5	0.0	6.119755818682957E-5	0.0	0.0	7.649694773353696E-6
6	0.0	6.119755818682957E-5	0.0	0.0	2.294908432006109E-5
7	0.0	6.884725296018326E-5	0.0	0.0	2.294908432006109E-5
8	0.0	9.179633728024436E-5	0.0	0.0	2.294908432006109E-5
9	0.0	9.944603205359804E-5	0.0	0.0	3.059877909341478E-5
10-11	0.0	9.944603205359804E-5	0.0	3.824847386676848E-6	3.824847386676848E-5
12-13	0.0	1.1857026898698228E-4	0.0	7.649694773353696E-6	3.824847386676848E-5
14-15	0.0	1.3004481114701282E-4	0.0	7.649694773353696E-6	4.972301602679903E-5
16-17	0.0	1.3004481114701282E-4	0.0	2.6773931706737934E-5	7.26721003468601E-5
18-19	0.0	1.4151935330704337E-4	0.0	3.442362648009163E-5	7.649694773353696E-5
20-21	0.0	1.5299389546707392E-4	0.0	6.119755818682957E-5	8.032179512021382E-5
22-23	7.649694773353696E-6	1.5299389546707392E-4	0.0	1.9506721672051924E-4	9.56211846669212E-5
24-25	7.649694773353696E-6	1.5681874285375076E-4	0.0	9.179633728024435E-4	1.0709572682695174E-4
26-27	7.649694773353696E-6	1.6446843762710445E-4	0.0	0.003040753672408094	1.0709572682695174E-4
28-29	7.649694773353696E-6	1.6829328501378132E-4	0.0	0.014893955723719645	1.0709572682695174E-4
30-31	7.649694773353696E-6	1.835926745604887E-4	0.0	0.020818644325682083	1.1092057421362858E-4
32-33	7.649694773353696E-6	1.835926745604887E-4	0.0	0.023343043600888805	1.2239511637365913E-4
34-35	7.649694773353696E-6	1.912423693338424E-4	0.0	0.02640292151023028	1.3769450592036653E-4
36-37	7.649694773353696E-6	2.1419145365390347E-4	0.0	0.03154734124531064	1.3769450592036653E-4
38-39	7.649694773353696E-6	2.2949084320061087E-4	0.0	0.04170613590432435	1.3769450592036653E-4
40-41	7.649694773353696E-6	2.4479023274731826E-4	0.0	0.06284989225787396	1.453442006937202E-4
42-43	7.649694773353696E-6	2.4479023274731826E-4	0.0	0.07213279686533867	1.529938954670739E-4
44-45	7.649694773353696E-6	2.5243992752067195E-4	0.0	0.08643390124412341	1.6829328501378132E-4
46-47	7.649694773353696E-6	2.6008962229402563E-4	0.0	0.104689897820732	1.8741752194716555E-4
48-49	7.649694773353696E-6	2.6008962229402563E-4	0.0	0.1193620123960244	1.912423693338424E-4
50-51	7.649694773353696E-6	2.6773931706737937E-4	0.0	0.13763330836217968	1.912423693338424E-4
52-53	7.649694773353696E-6	2.7156416445405624E-4	0.0	0.15949231117703788	2.065417588805498E-4
54-55	7.649694773353696E-6	3.251120278675321E-4	0.0	0.2291733808675167	2.3714053797396458E-4
56-57	7.649694773353696E-6	3.595356543476237E-4	0.0	0.28711981877567094	2.5243992752067195E-4
58-59	7.649694773353696E-6	3.824847386676848E-4	0.0	0.33320922978512696	2.600896222940257E-4
60-61	7.649694773353696E-6	3.9778412821439216E-4	0.0	0.6340526009841945	2.6773931706737937E-4
62-63	7.649694773353696E-6	4.0160897560106903E-4	0.0	0.6408187560112257	2.6773931706737937E-4
64-65	7.649694773353696E-6	4.5898168640122174E-4	0.0	0.6474127929058566	2.7156416445405624E-4
66-67	7.649694773353696E-6	4.895804654946365E-4	0.0	0.6528555507370979	2.8303870661408674E-4
68-69	7.649694773353696E-6	4.972301602679903E-4	0.0	0.6583289073474323	2.906884013874404E-4
70-71	7.649694773353696E-6	5.125295498146976E-4	0.0	0.6641044269013144	2.9833809616079416E-4
72-73	7.649694773353696E-6	5.354786341347586E-4	0.0	0.6685680238015663	3.212871804808552E-4
74-75	7.649694773353696E-6	5.431283289081124E-4	0.0	0.6726491359631506	3.212871804808552E-4
76-77	7.649694773353696E-6	5.507780236814661E-4	0.0	0.6760303010529729	3.251120278675321E-4
78-79	7.649694773353696E-6	5.660774132281735E-4	0.0	0.6792967207211948	3.289368752542089E-4
80-81	7.649694773353696E-6	5.737271080015272E-4	0.0	0.6806660160856252	3.289368752542089E-4
82-83	7.649694773353696E-6	5.737271080015272E-4	0.0	0.6811249977720264	3.480611121875932E-4
84-85	7.649694773353696E-6	5.737271080015272E-4	0.0	0.681484533426374	3.5188595957427E-4
86-87	7.649694773353696E-6	5.737271080015272E-4	0.0	0.6816490018640011	3.5188595957427E-4
88-89	7.649694773353696E-6	5.890264975482346E-4	0.0	0.6817407982012814	3.595356543476237E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17015	0.0	50.359283	1
GTATCAA	32070	0.0	37.153374	1
TCAACGC	39765	0.0	29.499277	4
ATCAACG	39700	0.0	29.472195	3
CAACGCA	40330	0.0	29.054302	5
TATCAAC	41315	0.0	28.550705	2
AACGCAG	41735	0.0	28.189295	6
ACGCAGA	48020	0.0	24.490921	7
CGCAGAG	48490	0.0	24.192001	8
GCAGAGT	56140	0.0	20.78922	9
GTACATG	36170	0.0	19.788717	1
GTGGTAT	8775	0.0	19.383902	1
GAGTACT	35340	0.0	19.190445	12-13
TACATGG	36905	0.0	19.175129	2
AGAGTAC	51710	0.0	19.112562	10-11
ACATGGG	36915	0.0	18.497808	3
CAGAGTA	55165	0.0	18.18599	10-11
TGGTATC	8985	0.0	17.46004	2
AGTACTT	38660	0.0	17.415628	12-13
GTACTTT	39435	0.0	17.181921	14-15
>>END_MODULE