FastQCFastQC Report
Fri 27 May 2016
SRR2139087_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139087_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9400065
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118800.052.7941471
GTATCAA224950.039.8361781
TCAACGC286000.030.8786224
ATCAACG287500.030.7024153
CAACGCA295150.029.921195
TATCAAC298400.029.8148732
AACGCAG306000.028.8891016
ACGCAGA347100.025.4436027
CGCAGAG352250.025.0594148
GTGGTAT54950.023.4532411
GCAGAGT409800.021.3936949
TGGTATC54250.020.9844592
CGAGACC68900.020.36509588-89
GTACATG271150.020.2791481
GAGACCG69750.019.99324888-89
AGACCGA69750.019.90102490-91
GACCGAG71950.019.82500590-91
GAGTACT248700.019.69901812-13
AGAGTAC369650.019.65682410-11
TACATGG277600.019.591422
Produced by FastQC (version 0.11.5)