##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139087_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9400065
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.575869315797284	34.0	31.0	34.0	31.0	34.0
2	32.71218401149354	34.0	31.0	34.0	31.0	34.0
3	32.696603800080105	34.0	31.0	34.0	31.0	34.0
4	36.22471546739305	37.0	35.0	37.0	35.0	37.0
5	36.1818300192605	37.0	35.0	37.0	35.0	37.0
6	36.22619013804692	37.0	37.0	37.0	35.0	37.0
7	36.186137542665925	37.0	37.0	37.0	35.0	37.0
8	36.16037474208955	37.0	37.0	37.0	35.0	37.0
9	37.89395126523061	39.0	38.0	39.0	35.0	39.0
10-11	37.82218761253247	39.0	38.0	39.0	35.0	39.0
12-13	37.79538710636575	39.0	38.0	39.0	35.0	39.0
14-15	39.14304698956869	40.0	39.0	41.0	36.0	41.0
16-17	39.11184396065347	40.0	38.0	41.0	36.0	41.0
18-19	39.07095488169497	40.0	38.5	41.0	36.0	41.0
20-21	39.05222952487141	40.0	39.0	41.0	36.0	41.0
22-23	38.937833743228175	40.0	38.0	41.0	35.0	41.0
24-25	38.80399835997517	40.0	38.0	41.0	35.0	41.0
26-27	38.754894989355975	40.0	38.0	41.0	34.5	41.0
28-29	38.7020909446553	40.0	38.0	41.0	35.0	41.0
30-31	38.6322766086801	40.0	38.0	41.0	35.0	41.0
32-33	38.519839395745294	40.0	38.0	41.0	34.0	41.0
34-35	38.32808165609932	40.0	38.0	41.0	34.0	41.0
36-37	38.46586157963415	40.0	38.0	41.0	34.0	41.0
38-39	38.447693722102116	40.0	38.0	41.0	34.0	41.0
40-41	38.40951229354894	40.0	38.0	41.0	34.0	41.0
42-43	38.44023012308939	40.0	38.0	41.0	34.0	41.0
44-45	38.373869036010085	40.0	38.0	41.0	34.0	41.0
46-47	38.313810180450176	40.0	38.0	41.0	34.0	41.0
48-49	38.15161313062987	40.0	38.0	41.0	33.0	41.0
50-51	38.1493103496287	40.0	37.5	41.0	33.0	41.0
52-53	38.05086266574246	40.0	37.0	41.0	33.0	41.0
54-55	37.89970057630589	40.0	37.0	41.0	33.0	41.0
56-57	37.7274296267077	40.0	36.5	41.0	33.0	41.0
58-59	37.557816277747015	39.0	36.0	41.0	33.0	41.0
60-61	37.37187557829524	39.0	36.0	41.0	32.0	41.0
62-63	37.15354326532521	39.0	35.0	40.5	32.0	41.0
64-65	36.85388673075123	38.0	35.0	40.0	31.5	41.0
66-67	36.49155315937274	38.0	35.0	40.0	31.0	41.0
68-69	36.1477564031686	37.0	35.0	40.0	31.0	41.0
70-71	35.84127637605722	37.0	34.0	39.0	30.5	41.0
72-73	35.50916888489564	36.0	34.0	39.0	30.0	40.5
74-75	35.13894947965133	36.0	34.0	38.5	30.0	40.0
76-77	34.19059626798409	35.0	33.0	37.0	29.0	39.0
78-79	34.47063198662147	35.0	34.0	37.0	29.5	39.0
80-81	34.271918493625606	35.0	34.0	36.5	29.5	39.0
82-83	33.99683252860354	35.0	33.5	36.0	29.0	38.0
84-85	33.760481535373174	35.0	33.0	36.0	29.0	37.0
86-87	33.529371150263984	35.0	33.0	35.0	29.0	37.0
88-89	33.29848413328919	35.0	33.0	35.0	29.0	36.0
90-91	33.11415453126928	35.0	33.0	35.0	29.0	36.0
92-93	33.03094854953755	35.0	33.0	35.0	29.0	36.0
94-95	32.95394039913964	34.0	32.5	35.0	29.0	35.5
96-97	32.938676262271386	34.0	32.0	35.0	29.0	35.0
98-99	33.06828035564861	34.0	32.5	35.0	29.0	35.0
100-101	31.219420937629977	32.5	29.0	34.5	24.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	6.0
20	42.0
21	202.0
22	1062.0
23	3843.0
24	9828.0
25	21296.0
26	38219.0
27	62030.0
28	90044.0
29	124221.0
30	161712.0
31	206758.0
32	264775.0
33	346524.0
34	483277.0
35	699738.0
36	1160816.0
37	2116628.0
38	2472943.0
39	1078359.0
40	57741.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.695176830197845	21.323016664888307	15.69571395425754	24.28609255065631
2	13.746671336831987	21.711055226123534	41.26259167788457	23.279681759159914
3	17.051438910569004	26.490865008032394	31.227854536719597	25.22984154467901
4	12.094851845985058	18.802104116691307	38.845286870313124	30.257757167010514
5	13.040859407006103	37.937458504267475	35.65829292846229	13.363389160264138
6	29.750574038644846	38.35898351330374	18.610128611890303	13.280313836161115
7	26.207526481471454	33.038204105653335	23.094143092445233	17.66012632042998
8	24.40176198819093	36.95522040650153	20.912998136289087	17.73001946901845
9	25.42233755004221	16.97005467196819	21.3194505909747	36.288157187014896
10-11	23.97060908937815	27.052773873838316	28.908881219661726	20.067735817121804
12-13	24.853918983992195	24.627637225570005	29.06685224100643	21.451591549431377
14-15	21.9898508953312	26.019413106815172	26.8241010863676	25.166634911486025
16-17	21.455257939463056	29.137520904522184	27.48712217602778	21.920098979986978
18-19	21.49096841351629	28.21533680884122	29.375068151124488	20.91862662651801
20-21	22.220277737646928	27.251232631822404	29.45719305270768	21.07129657782299
22-23	21.95713897988142	27.21612963251997	29.259458568634543	21.567272818964067
24-25	21.781320519554434	27.36110689118237	29.28119427643216	21.576378312831043
26-27	21.72365638832886	27.47323939718196	29.219978886537685	21.5831253279515
28-29	21.673838488391308	27.507268037885428	29.23720344206837	21.581690031654894
30-31	21.909227428560307	27.41680836631282	29.134463112992993	21.539501092133882
32-33	21.738526567525405	27.52420763638191	29.10503011271526	21.63223568337743
34-35	21.87729316798049	27.55153671283833	29.08360488875926	21.487565230421918
36-37	21.838503027448006	27.51497677316022	29.024031915757742	21.62248828363403
38-39	21.774122578250388	27.64809119722341	29.007766788781364	21.57001943574484
40-41	22.006329113236532	27.499733046207286	28.871522499126357	21.622415341429825
42-43	21.828454351881792	27.653125856098303	28.67219576356918	21.846224028450724
44-45	21.96100531087654	27.697169576449344	28.492029828704275	21.84979528396984
46-47	22.030558787833638	27.74823108061002	28.15684353729394	22.064366594262403
48-49	21.970576489288813	27.90862439874548	28.07245288402797	22.04834622793774
50-51	22.003386816169577	27.98699751936767	27.959826132510806	22.04978953195195
52-53	22.145023623601922	27.89412666074334	27.865226974065106	22.09562274158963
54-55	22.082920322296363	27.804447072239498	27.91980572235716	22.192826883106974
56-57	22.11374689267518	27.904855525258448	27.86486465284485	22.11653292922152
58-59	22.059774224953067	27.967481782061665	27.89584904379651	22.07689494918876
60-61	22.123933501968462	27.87017314654972	27.93408885047989	22.071804501001928
62-63	22.199795688402382	27.93156556484691	27.91572791290775	21.952910833842953
64-65	22.014905080508857	27.88752983711565	27.889002248752547	22.208562833622945
66-67	22.079131473912085	27.927368231728263	27.72923658694793	22.26426370741172
68-69	22.010163334462565	28.04318775674759	27.80380378272308	22.142845126066764
70-71	22.025462256363866	27.877807765079467	27.82345539882347	22.27327457973319
72-73	22.044722263550476	27.89596560902475	27.6385380787067	22.42077404871808
74-75	21.981395712281284	27.930746102587978	27.6289331874628	22.458924997667935
76-77	22.134627844425538	27.731132528524977	27.73500337298463	22.39923625406486
78-79	22.064711336014003	27.695049619299816	27.697873342286993	22.542365702399188
80-81	22.21934451689516	27.78993602918941	27.5346084619286	22.45611099198683
82-83	22.26358229004646	27.894820184435957	27.604233486340355	22.237364039177223
84-85	22.16947704403199	27.72396624255936	27.59146809058242	22.51508862282623
86-87	22.057128475248703	27.896684067863536	27.654285735424356	22.391901721463405
88-89	22.23772452661882	27.659138520590897	27.653164659611562	22.449972293178718
90-91	22.306104486251094	27.832336928405127	27.6794112085331	22.182147376810676
92-93	22.180046911850564	27.691128377704892	27.745691812985836	22.38313289745871
94-95	22.16902579700808	27.582202545523444	27.81615594444904	22.43261571301944
96-97	22.08922180822536	27.783088017756608	27.90528068892502	22.22240948509301
98-99	22.265998084277943	28.24258233802585	27.98854561054615	21.50287396715005
100-101	20.788771108380335	31.824017110288878	25.802922800914008	21.58428898041678
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2429.0
1	2092.5
2	2494.5
3	3417.5
4	4511.0
5	6680.0
6	8609.0
7	10990.0
8	13427.5
9	14165.0
10	13207.0
11	11994.0
12	13048.5
13	17625.5
14	28650.0
15	43775.0
16	55682.0
17	61649.0
18	61994.0
19	58244.0
20	52637.5
21	47032.5
22	43772.0
23	45858.0
24	53171.5
25	65184.5
26	80738.0
27	97907.5
28	117323.0
29	138368.5
30	159614.5
31	181009.0
32	203267.0
33	225912.0
34	246864.0
35	266179.5
36	288528.0
37	307865.0
38	322657.0
39	335756.5
40	347369.0
41	358998.5
42	365145.5
43	369832.5
44	376574.0
45	381338.5
46	382034.5
47	380410.0
48	378708.0
49	375104.5
50	367650.5
51	354973.5
52	337405.0
53	319095.0
54	301511.0
55	281959.5
56	259605.5
57	235133.0
58	208484.0
59	178903.0
60	148204.5
61	120163.0
62	95004.5
63	72219.0
64	53476.0
65	39948.5
66	30001.5
67	22639.0
68	17000.0
69	12164.5
70	8575.0
71	6062.5
72	4209.0
73	2835.5
74	1819.5
75	1156.5
76	746.0
77	475.5
78	298.0
79	186.0
80	119.0
81	86.5
82	70.0
83	49.5
84	35.0
85	27.5
86	24.0
87	23.5
88	18.0
89	10.5
90	5.0
91	2.5
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.1276448620302095E-5
2	0.014861599361281012
3	2.1276448620302095E-5
4	1.7021158896241676E-4
5	2.1276448620302095E-5
6	1.0638224310151048E-5
7	2.1276448620302095E-5
8	2.1276448620302095E-5
9	1.0638224310151048E-5
10-11	1.7021158896241676E-4
12-13	6.223361221438363E-4
14-15	2.553173834436251E-4
16-17	0.0019680714973779436
18-19	0.0
20-21	0.0
22-23	1.7554798500437195E-4
24-25	0.009135495844067674
26-27	0.019218914775848694
28-29	0.3411444763816461
30-31	0.3174251077301632
32-33	0.3112004114951444
34-35	0.18283428474758837
36-37	0.04380411671647737
38-39	0.035593684402664964
40-41	0.013924195679480656
42-43	0.012619195791604775
44-45	0.016508541972403357
46-47	0.011473656673282179
48-49	0.006325716882334543
50-51	0.0029611865858925794
52-53	0.0011038545634058897
54-55	7.44507439318778E-5
56-57	1.3266823095970065E-4
58-59	8.426626256054022E-4
60-61	7.125706218814084E-4
62-63	1.2363950911111317E-4
64-65	4.155998860781341E-5
66-67	0.0033261564266795864
68-69	0.005337244892401869
70-71	0.006648464458305053
72-73	0.010403146612483097
74-75	0.007557993110450007
76-77	0.005311901113044682
78-79	0.0033832653267324895
80-81	0.00443105538285444
82-83	0.0015548547215961058
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0023533869114225644
92-93	0.014453696004086955
94-95	0.0012953353124918153
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	154.0
20-21	530.0
22-23	1151.0
24-25	2979.0
26-27	3932.0
28-29	6174.0
30-31	16395.0
32-33	21875.0
34-35	31598.0
36-37	43676.0
38-39	51109.0
40-41	56140.0
42-43	67444.0
44-45	81747.0
46-47	80872.0
48-49	70657.0
50-51	62066.0
52-53	55733.0
54-55	61223.0
56-57	64171.0
58-59	42753.0
60-61	68364.0
62-63	69491.0
64-65	76576.0
66-67	80468.0
68-69	80267.0
70-71	79825.0
72-73	78061.0
74-75	80512.0
76-77	80411.0
78-79	86837.0
80-81	89065.0
82-83	89167.0
84-85	92850.0
86-87	100107.0
88-89	109360.0
90-91	120226.0
92-93	132533.0
94-95	160246.0
96-97	249511.0
98-99	951763.0
100-101	5702046.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.29355039186173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.7020682228229	78.27003494832249
2	6.564779506304077	11.32996262277424
3	1.5197155426254667	3.9342494927653857
4	0.5527525836494238	1.9079593172553324
5	0.2641931823312108	1.1399083846342328
6	0.12512766296214572	0.6478626173543888
7	0.07354773889032673	0.44426868584979284
8	0.04809968597091341	0.33205541401309924
9	0.03490213191307802	0.27106459911221426
>10	0.1118606900430193	1.503868805458252
>50	0.002454907153536102	0.13929967179255937
>100	4.785737369780053E-4	0.06850446292867075
>500	1.9571596852675352E-5	0.010960977739368475
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.0638224310151048E-5	0.0	0.0	0.0
3	0.0	1.0638224310151048E-5	0.0	1.0638224310151048E-5	0.0
4	0.0	8.510579448120838E-5	0.0	1.0638224310151048E-5	0.0
5	0.0	8.510579448120838E-5	0.0	1.0638224310151048E-5	0.0
6	4.255289724060419E-5	8.510579448120838E-5	0.0	1.0638224310151048E-5	0.0
7	4.255289724060419E-5	9.574401879135942E-5	0.0	3.191467293045314E-5	0.0
8	4.255289724060419E-5	9.574401879135942E-5	0.0	3.191467293045314E-5	0.0
9	4.255289724060419E-5	1.7021158896241676E-4	0.0	5.319112155075523E-5	0.0
10-11	4.7872009395679715E-5	1.7021158896241676E-4	0.0	9.04249066362839E-5	0.0
12-13	5.319112155075523E-5	1.7021158896241676E-4	0.0	1.0638224310151046E-4	0.0
14-15	6.382934586090628E-5	2.8191294421900273E-4	0.0	1.0638224310151046E-4	5.319112155075524E-6
16-17	8.510579448120838E-5	2.925511685291538E-4	0.0	1.1702046741166151E-4	1.0638224310151048E-5
18-19	8.510579448120838E-5	3.45742290079909E-4	0.0	1.382969160319636E-4	1.595733646522657E-5
20-21	8.510579448120838E-5	4.042525237857398E-4	0.0	1.7021158896241673E-4	2.1276448620302095E-5
22-23	8.510579448120838E-5	4.734009818017216E-4	0.0	3.4042317792483347E-4	2.1276448620302095E-5
24-25	9.04249066362839E-5	5.478685519727789E-4	0.0	9.04249066362839E-4	2.1276448620302095E-5
26-27	9.574401879135942E-5	5.744641127481565E-4	0.0	0.0027712574327943477	2.1276448620302095E-5
28-29	9.574401879135942E-5	5.79783224903232E-4	0.0	0.014425432164564819	2.1276448620302095E-5
30-31	9.574401879135942E-5	6.70208131539516E-4	0.0	0.020563687591521974	2.1276448620302095E-5
32-33	9.574401879135942E-5	6.80846355849667E-4	0.0	0.022382823948557805	2.1276448620302095E-5
34-35	9.574401879135942E-5	7.659521503308753E-4	0.0	0.025148762269197072	2.1276448620302095E-5
36-37	9.574401879135942E-5	8.404197205019327E-4	0.0	0.029643412040235892	2.1276448620302095E-5
38-39	1.0106313094643493E-4	8.776535055874614E-4	0.0	0.038419947096110504	2.1276448620302095E-5
40-41	1.0638224310151046E-4	9.893548608440473E-4	0.0	0.057042158751029914	2.1276448620302095E-5
42-43	1.1702046741166151E-4	0.001037226870239727	0.0	0.06483465805821556	2.1276448620302095E-5
44-45	1.2233957956673703E-4	0.0012127575713572194	0.0	0.07736116718341841	2.1276448620302095E-5
46-47	1.2765869172181256E-4	0.001351054487389183	0.0	0.09404722201389033	2.1276448620302095E-5
48-49	1.2765869172181256E-4	0.001361692711699334	0.0	0.10738755529881974	2.1276448620302095E-5
50-51	1.542542524971902E-4	0.0014680749548008445	0.0	0.12376510162429727	2.1276448620302095E-5
52-53	1.7021158896241676E-4	0.001484032291266071	0.0	0.14259475865326462	2.1276448620302095E-5
54-55	1.7553070111749226E-4	0.001505308739886373	0.0	0.20359965595982582	2.1276448620302095E-5
56-57	1.808498132725678E-4	0.0015319043006617507	0.0	0.2543865388164869	2.1276448620302095E-5
58-59	1.808498132725678E-4	0.0015531807492820528	0.0	0.29552987133599606	2.1276448620302095E-5
60-61	1.9148803758271883E-4	0.0015584998614371283	0.0	0.5726077426060352	3.191467293045314E-5
62-63	2.1276448620302093E-4	0.0015638189735922038	0.0	0.5783417455092066	3.191467293045314E-5
64-65	2.1808359835809646E-4	0.0017712643476401493	0.0	0.5845757449549551	3.191467293045314E-5
66-67	2.23402710513172E-4	0.001797859908415527	0.0	0.5898044322033944	3.191467293045314E-5
68-69	2.23402710513172E-4	0.001840412805656131	0.0	0.5944533362269303	4.7872009395679715E-5
70-71	2.553173834436251E-4	0.0018776465907416598	0.0	0.5989958580073649	5.319112155075523E-5
72-73	3.191467293045314E-4	0.0018936039272068864	0.0	0.602814980534709	5.319112155075523E-5
74-75	3.191467293045314E-4	0.001930837712292415	0.0	0.6062032549774922	5.319112155075523E-5
76-77	3.191467293045314E-4	0.0019787097216880946	0.0	0.6091500431114041	5.319112155075523E-5
78-79	3.191467293045314E-4	0.0020053052824634725	0.0	0.6120915121331608	5.319112155075523E-5
80-81	3.191467293045314E-4	0.002010624394618548	0.0	0.6133468226017587	5.319112155075523E-5
82-83	3.191467293045314E-4	0.002015943506773623	0.0	0.6138149044714052	5.319112155075523E-5
84-85	3.191467293045314E-4	0.002021262618928699	0.0	0.6140914983034692	5.319112155075523E-5
86-87	3.191467293045314E-4	0.002063815516169303	0.0	0.6142404334438112	5.319112155075523E-5
88-89	3.191467293045314E-4	0.0021701977592708138	0.0	0.6142723481167417	5.319112155075523E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11880	0.0	52.794147	1
GTATCAA	22495	0.0	39.836178	1
TCAACGC	28600	0.0	30.878622	4
ATCAACG	28750	0.0	30.702415	3
CAACGCA	29515	0.0	29.92119	5
TATCAAC	29840	0.0	29.814873	2
AACGCAG	30600	0.0	28.889101	6
ACGCAGA	34710	0.0	25.443602	7
CGCAGAG	35225	0.0	25.059414	8
GTGGTAT	5495	0.0	23.453241	1
GCAGAGT	40980	0.0	21.393694	9
TGGTATC	5425	0.0	20.984459	2
CGAGACC	6890	0.0	20.365095	88-89
GTACATG	27115	0.0	20.279148	1
GAGACCG	6975	0.0	19.993248	88-89
AGACCGA	6975	0.0	19.901024	90-91
GACCGAG	7195	0.0	19.825005	90-91
GAGTACT	24870	0.0	19.699018	12-13
AGAGTAC	36965	0.0	19.656824	10-11
TACATGG	27760	0.0	19.59142	2
>>END_MODULE