FastQCFastQC Report
Fri 27 May 2016
SRR2139086_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139086_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11271375
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151250.052.344361
GTATCAA274650.036.658081
TCAACGC346100.028.7281914
ATCAACG347600.028.5672633
CAACGCA354600.028.02745
TATCAAC359000.027.8428572
AACGCAG363550.027.4200616
ACGCAGA415400.023.9564117
CGCAGAG427300.023.2792578
GTGGTAT74350.021.0097791
GCAGAGT493250.020.0629739
TGGTATC73000.020.0536122
GTACATG340850.019.9843941
GAGTACT290800.019.80403112-13
TACATGG344600.019.3334332
AGAGTAC448050.018.75410310-11
ACATGGG347000.018.6555543
CAGAGTA481250.018.28056710-11
GTACTTT322350.017.83285314-15
AGTACTT317150.017.33783512-13
Produced by FastQC (version 0.11.5)