##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139086_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11271375
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5749688924377	34.0	31.0	34.0	31.0	34.0
2	32.711888212396445	34.0	31.0	34.0	31.0	34.0
3	32.697131450244534	34.0	31.0	34.0	31.0	34.0
4	36.22432356300806	37.0	35.0	37.0	35.0	37.0
5	36.183306207095406	37.0	35.0	37.0	35.0	37.0
6	36.22658575373457	37.0	37.0	37.0	35.0	37.0
7	36.18677667986381	37.0	37.0	37.0	35.0	37.0
8	36.16349061228111	37.0	37.0	37.0	35.0	37.0
9	37.89788787969525	39.0	38.0	39.0	35.0	39.0
10-11	37.82749935123266	39.0	38.0	39.0	35.0	39.0
12-13	37.79863344090673	39.0	38.0	39.0	35.0	39.0
14-15	39.14536735757616	40.0	39.0	41.0	36.0	41.0
16-17	39.11367628173138	40.0	38.0	41.0	36.0	41.0
18-19	39.077229264397644	40.0	38.5	41.0	36.0	41.0
20-21	39.06299594127053	40.0	39.0	41.0	36.0	41.0
22-23	38.94801968140101	40.0	38.0	41.0	35.0	41.0
24-25	38.8135642852436	40.0	38.0	41.0	35.0	41.0
26-27	38.764065241653185	40.0	38.0	41.0	34.5	41.0
28-29	38.703104511398465	40.0	38.0	41.0	35.0	41.0
30-31	38.63448820871925	40.0	38.0	41.0	35.0	41.0
32-33	38.521749912812155	40.0	38.0	41.0	34.0	41.0
34-35	38.328016194803766	40.0	38.0	41.0	34.0	41.0
36-37	38.46642416196812	40.0	38.0	41.0	34.0	41.0
38-39	38.446191990878575	40.0	38.0	41.0	34.0	41.0
40-41	38.41641126393743	40.0	38.0	41.0	34.0	41.0
42-43	38.44933203184979	40.0	38.0	41.0	34.0	41.0
44-45	38.38328794120717	40.0	38.0	41.0	34.0	41.0
46-47	38.3224737004261	40.0	38.0	41.0	34.0	41.0
48-49	38.15866038569867	40.0	38.0	41.0	33.0	41.0
50-51	38.153963179308164	40.0	37.0	41.0	33.0	41.0
52-53	38.06210229553767	40.0	37.0	41.0	33.0	41.0
54-55	37.911622220737115	40.0	37.0	41.0	33.0	41.0
56-57	37.74370033303633	40.0	36.5	41.0	33.0	41.0
58-59	37.57597070275386	39.0	36.0	41.0	33.0	41.0
60-61	37.39597788904296	39.0	36.0	41.0	32.0	41.0
62-63	37.18209943232203	39.0	35.0	40.5	32.0	41.0
64-65	36.887455527531934	38.0	35.0	40.0	31.5	41.0
66-67	36.53578606356787	38.0	35.0	40.0	31.0	41.0
68-69	36.195872905320755	37.0	35.0	40.0	31.0	41.0
70-71	35.89074170202831	37.0	34.5	39.0	31.0	41.0
72-73	35.562288130919654	36.0	34.0	39.0	30.0	40.5
74-75	35.19360150878482	36.0	34.0	38.5	30.0	40.0
76-77	34.23514948829306	35.0	33.0	37.0	29.0	39.0
78-79	34.519094909063746	35.0	34.0	37.0	30.0	39.0
80-81	34.32164529913649	35.0	34.0	37.0	30.0	39.0
82-83	34.047567247973504	35.0	34.0	36.0	29.0	38.5
84-85	33.80586592959169	35.0	33.0	36.0	29.0	37.0
86-87	33.574668175990695	35.0	33.0	35.5	29.0	37.0
88-89	33.34266509001867	35.0	33.0	35.0	29.0	36.5
90-91	33.15903847706767	35.0	33.0	35.0	29.0	36.0
92-93	33.07467837794773	35.0	33.0	35.0	29.0	36.0
94-95	32.99671510645958	34.0	33.0	35.0	29.0	35.5
96-97	32.97993049368901	34.0	32.0	35.0	29.0	35.0
98-99	33.100876694476625	34.0	32.5	35.0	29.0	35.0
100-101	31.241621275122064	32.5	29.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	9.0
20	50.0
21	264.0
22	1374.0
23	4564.0
24	12135.0
25	25328.0
26	46328.0
27	73561.0
28	107197.0
29	146736.0
30	191533.0
31	243253.0
32	310602.0
33	407049.0
34	567248.0
35	823637.0
36	1372164.0
37	2531736.0
38	2986229.0
39	1346919.0
40	73458.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.489554291290986	21.559836311009086	15.74166417140766	24.208945226292265
2	13.631959593969798	21.744772775975733	41.50356645153047	23.119701178524004
3	16.78784531612159	26.8142706635171	31.53263022479511	24.865253795566204
4	11.918981248590455	19.015814054835943	39.03056648147172	30.03463821510188
5	12.807700923800336	38.14201905268878	35.87159508045824	13.178684943052643
6	29.34971110445709	38.70669727517716	18.73828170919697	13.205309911168781
7	25.870330815894242	33.218218717769574	23.278340042807557	17.633110423528628
8	24.277934147342272	36.96755719688148	21.175065153985205	17.579443501791044
9	25.291404109968834	16.940728172028702	21.43434141797252	36.333526300029945
10-11	23.800394051914335	27.12213679674343	29.11194148530911	19.96552766603313
12-13	24.676499075483505	24.6802164826284	29.176025616217906	21.467258825670196
14-15	21.86992698338356	26.09436758553565	26.923896394381195	25.11180903669959
16-17	21.30819793403861	29.288102236777746	27.546388489073315	21.85731134011033
18-19	21.343030464339975	28.309101595856763	29.405374233400984	20.942493706402278
20-21	22.175878405876425	27.413481824398055	29.34874157034411	21.061898199381407
22-23	21.892712953842693	27.367507182895174	29.21454561006451	21.52523425319762
24-25	21.698762409641205	27.50766357597097	29.33539710075262	21.458176913635203
26-27	21.678942474368643	27.68686135259341	29.211856962959033	21.42233921007891
28-29	21.493212447881383	27.63265465090609	29.25950679298868	21.61462610822384
30-31	21.888630024510828	27.554920576959002	29.156885296193625	21.399564102336544
32-33	21.774106992449855	27.626510799290966	29.12689217152501	21.47249003673417
34-35	21.667537943034187	27.660939333478503	29.253696972853856	21.417825750633455
36-37	21.7692237596126	27.611002610052548	29.0947344580104	21.52503917232444
38-39	21.63816194717377	27.715718648386982	29.110312470568196	21.535806933871047
40-41	21.817371904899336	27.593964552286632	29.022574286930357	21.566089255883675
42-43	21.769576006351166	27.694985263728416	28.82045047480699	21.714988255113425
44-45	21.793921105632847	27.881426966424666	28.561251172158364	21.763400755784122
46-47	21.954458321063917	27.720640444137846	28.24423524391286	22.08066599088537
48-49	21.92747942379342	27.83713548866421	28.22422717268563	22.011157914856735
50-51	21.82964957363533	28.00877936056757	28.1271098313953	22.034461234401807
52-53	22.002157019044322	27.950971168875586	28.045062945486098	22.00180886659399
54-55	21.9890345793375	27.884308525391006	27.98142215054163	22.145234744729866
56-57	22.04780804290084	28.0664638909015	27.848922696467177	22.03680536973048
58-59	21.896103026334636	28.144422008637854	28.03873084298809	21.92074412203942
60-61	21.99332734160119	28.10353430377925	27.928642358194967	21.974495996424597
62-63	22.05735690430562	28.232891809760257	27.856843159698236	21.85290812623589
64-65	21.864535577991	28.13198164634731	27.889017415728457	22.114465359933238
66-67	21.97881149180291	28.122894600989962	27.727361359933226	22.1709325472739
68-69	21.909068609556098	28.179007121317657	27.827838450353536	22.084085818772706
70-71	21.905152195626172	27.95706635290585	27.90829548260524	22.22948596886274
72-73	22.008418605422566	27.99369366052142	27.67762044322954	22.320267290826475
74-75	21.897465809290768	28.062194528221955	27.62204644751734	22.418293214969935
76-77	22.007735350768133	27.836852619384405	27.75556828320879	22.399843746638666
78-79	21.948596005045626	27.833458390193876	27.730637050359846	22.487308554400652
80-81	22.14969971958724	27.849151137074614	27.55772620804919	22.44342293528896
82-83	22.213313606140915	27.958119840709756	27.657852262553323	22.17071429059601
84-85	22.14720752743844	27.748191099993846	27.589229146375704	22.51537222619201
86-87	21.960683971168343	27.983815547740843	27.663216423960723	22.392284057130095
88-89	22.2003603653169	27.717078840228876	27.706659881161972	22.375900913292256
90-91	22.27823531743355	27.87870928553639	27.722151740219942	22.120903656810125
92-93	22.11749106866089	27.79272470604846	27.724154669081756	22.365629556208894
94-95	22.11221126099049	27.63087480357726	27.823492304480858	22.433421630951393
96-97	22.01562873827343	27.906107450269047	27.923648698517393	22.154615112940128
98-99	22.21047528132986	28.28027804240249	28.02562150717914	21.483625169088512
100-101	20.706392832097173	31.909514355121164	25.839751419620683	21.54434139316098
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2777.0
1	2369.5
2	2970.0
3	4231.5
4	5705.5
5	8621.5
6	11084.0
7	13591.0
8	15765.5
9	16132.0
10	14871.0
11	13779.0
12	15546.5
13	21236.5
14	34943.5
15	53614.0
16	68301.0
17	76253.0
18	77065.0
19	72200.5
20	65060.5
21	58136.0
22	54236.0
23	56511.5
24	65526.5
25	80945.0
26	101718.0
27	124341.5
28	147892.5
29	173128.0
30	197860.5
31	223766.5
32	252890.5
33	282066.5
34	308621.0
35	333627.5
36	360532.0
37	383304.0
38	399151.5
39	412395.5
40	426226.0
41	438102.5
42	443922.5
43	450135.0
44	455338.0
45	457067.0
46	457532.5
47	454625.5
48	451816.0
49	452508.0
50	449167.0
51	432009.5
52	407021.0
53	383874.5
54	359269.5
55	330534.0
56	299647.0
57	268128.5
58	236785.0
59	203824.0
60	170234.0
61	138134.5
62	108254.0
63	82603.0
64	61418.5
65	45754.5
66	34894.5
67	27181.5
68	21100.5
69	15591.0
70	11265.0
71	8027.0
72	5573.0
73	3743.5
74	2456.5
75	1574.5
76	1014.5
77	654.0
78	437.5
79	299.5
80	203.5
81	140.5
82	100.5
83	68.5
84	45.5
85	36.0
86	29.5
87	23.5
88	14.0
89	7.5
90	5.0
91	4.0
92	5.0
93	4.5
94	2.5
95	1.5
96	1.0
97	0.5
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.01570349668962305
3	0.0
4	7.98482882523206E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.5526056049062337E-4
12-13	6.920184981867785E-4
14-15	3.238291690233002E-4
16-17	0.0018276385977753378
18-19	0.0
20-21	0.0
22-23	2.440062201178337E-4
24-25	0.009336050194936638
26-27	0.019326194063840094
28-29	0.3485923839535954
30-31	0.32421192520228403
32-33	0.31770468697260235
34-35	0.1863121162103065
36-37	0.043820411566543614
38-39	0.035549094634359256
40-41	0.014348782567268027
42-43	0.012990827397855965
44-45	0.01647385135426483
46-47	0.011025554770327227
48-49	0.0063067193845167836
50-51	0.0031815126842933833
52-53	0.0011589049997880112
54-55	7.203913396282089E-5
56-57	1.742611279767915E-4
58-59	8.737554659139938E-4
60-61	8.787229318359895E-4
62-63	1.9812328706939947E-4
64-65	5.000393280931545E-5
66-67	0.003345782474407291
68-69	0.0053184418324418005
70-71	0.007015450717155611
72-73	0.010763574437053062
74-75	0.007871305109804706
76-77	0.0059209190970300205
78-79	0.0036172682573137314
80-81	0.004683492822601708
82-83	0.0016644817129126191
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.002619118529256689
92-93	0.015378217397542552
94-95	0.001316715656945077
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	170.0
20-21	710.0
22-23	1447.0
24-25	3520.0
26-27	4688.0
28-29	8011.0
30-31	21008.0
32-33	28304.0
34-35	41384.0
36-37	56218.0
38-39	66673.0
40-41	72582.0
42-43	87768.0
44-45	104786.0
46-47	103311.0
48-49	89432.0
50-51	79202.0
52-53	71410.0
54-55	79318.0
56-57	86231.0
58-59	57231.0
60-61	90973.0
62-63	93290.0
64-65	101837.0
66-67	109834.0
68-69	101270.0
70-71	98818.0
72-73	97169.0
74-75	99940.0
76-77	99681.0
78-79	106452.0
80-81	109788.0
82-83	108521.0
84-85	112748.0
86-87	120868.0
88-89	131218.0
90-91	144212.0
92-93	157892.0
94-95	189339.0
96-97	290653.0
98-99	1115363.0
100-101	6728105.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.53316415079561
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.41377228071119	75.58429089547218
2	7.31711011949362	12.370769416812037
3	1.8406158624005953	4.667792485046033
4	0.6601593639832767	2.2322143952513263
5	0.30288497202361375	1.280191252944064
6	0.1577848088559067	0.8002829488510962
7	0.08292828927683302	0.4907133483128232
8	0.057790649554339026	0.3908181172126436
9	0.039151500551576034	0.29786402005887075
>10	0.12473044259576963	1.5773397389298487
>50	0.0024542715361578022	0.14005504234816288
>100	4.973107705978033E-4	0.07896221206767058
>500	8.58096420994423E-5	0.04700338608606778
>1k	3.431860438290524E-5	0.04170274060710672
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.7744064056071243E-5	0.0	0.0	0.0
3	0.0	1.7744064056071243E-5	0.0	8.872032028035622E-6	0.0
4	0.0	5.323219216821373E-5	0.0	2.6616096084106865E-5	0.0
5	0.0	8.872032028035621E-5	0.0	2.6616096084106865E-5	0.0
6	3.5488128112142486E-5	8.872032028035621E-5	0.0	3.5488128112142486E-5	0.0
7	3.5488128112142486E-5	8.872032028035621E-5	0.0	3.5488128112142486E-5	0.0
8	3.5488128112142486E-5	9.759235230839183E-5	0.0	5.323219216821373E-5	0.0
9	3.5488128112142486E-5	1.4195251244856995E-4	0.0	7.984828825232058E-5	0.0
10-11	3.5488128112142486E-5	1.4638852846258777E-4	0.0	1.242084483924987E-4	0.0
12-13	3.5488128112142486E-5	1.5082454447660557E-4	0.0	1.4638852846258777E-4	0.0
14-15	5.7668208182231535E-5	2.1736478468687272E-4	0.0	1.5969657650464117E-4	4.436016014017811E-6
16-17	1.1533641636446307E-4	2.2180080070089054E-4	0.0	1.9518470461678367E-4	8.872032028035622E-6
18-19	1.1533641636446307E-4	2.3954486475696177E-4	0.0	2.7946900888312207E-4	8.872032028035622E-6
20-21	1.1533641636446307E-4	2.617249448270508E-4	0.0	3.5044526510740707E-4	8.872032028035622E-6
22-23	1.1533641636446307E-4	2.705969768550864E-4	0.0	5.234498896541017E-4	1.3308048042053432E-5
24-25	1.1533641636446307E-4	2.839050248971399E-4	0.0	0.0012509565159530226	1.7744064056071243E-5
26-27	1.1533641636446307E-4	2.927770569251755E-4	0.0	0.0037395614998170144	1.7744064056071243E-5
28-29	1.1533641636446307E-4	2.927770569251755E-4	0.0	0.0168612968692817	1.7744064056071243E-5
30-31	1.1533641636446307E-4	3.371372170653536E-4	0.0	0.02330239212163556	1.7744064056071243E-5
32-33	1.1977243237848088E-4	3.5044526510740707E-4	0.0	0.025817613201583656	1.7744064056071243E-5
34-35	1.3308048042053432E-4	4.1698550531767424E-4	0.0	0.029002672699648444	1.7744064056071243E-5
36-37	1.3308048042053432E-4	4.7021769748588794E-4	0.0	0.03418393940402125	1.7744064056071243E-5
38-39	1.3308048042053432E-4	5.057058255980304E-4	0.0	0.04443113639640239	1.7744064056071243E-5
40-41	1.3308048042053432E-4	5.234498896541016E-4	0.0	0.06468154950039369	1.7744064056071243E-5
42-43	1.3308048042053432E-4	5.456299697241907E-4	0.0	0.07373102216899002	1.7744064056071243E-5
44-45	1.3751649643455212E-4	6.254782579765113E-4	0.0	0.0875181599405574	1.7744064056071243E-5
46-47	1.4195251244856995E-4	6.831464661587428E-4	0.0	0.10561266926173604	2.2180080070089056E-5
48-49	1.4638852846258777E-4	7.05326546228832E-4	0.0	0.11986115269876124	2.6616096084106865E-5
50-51	1.5969657650464117E-4	7.851748344811525E-4	0.0	0.137729425203225	2.6616096084106865E-5
52-53	1.5969657650464117E-4	7.896108504951703E-4	0.0	0.15806855862749664	3.99241441261603E-5
54-55	1.6413259251865897E-4	8.117909305652593E-4	0.0	0.22138381519557285	5.323219216821373E-5
56-57	1.685686085326768E-4	8.339710106353484E-4	0.0	0.27570726730323497	5.323219216821373E-5
58-59	1.685686085326768E-4	8.384070266493662E-4	0.0	0.3175433343314369	5.323219216821373E-5
60-61	1.7744064056071242E-4	8.517150746914197E-4	0.0	0.6130174889931352	5.323219216821373E-5
62-63	1.8631267258874804E-4	8.65023122733473E-4	0.0	0.6189440063878631	6.210422419624935E-5
64-65	1.8631267258874804E-4	9.626154750418649E-4	0.0	0.6250390923911235	6.210422419624935E-5
66-67	2.040567366448193E-4	9.892315711259717E-4	0.0	0.6302247951115103	6.210422419624935E-5
68-69	2.040567366448193E-4	0.0010114116511960609	0.0	0.6354415499439954	6.210422419624935E-5
70-71	2.1736478468687272E-4	0.0010202836832240965	0.0	0.6405607124241719	7.097625622428497E-5
72-73	2.3954486475696177E-4	0.0010202836832240965	0.0	0.6446817713011944	7.097625622428497E-5
74-75	2.3954486475696177E-4	0.0010468997793082035	0.0	0.6481418637921283	7.097625622428497E-5
76-77	2.5728892881303304E-4	0.0010912599394483813	0.0	0.6513934635304033	7.097625622428497E-5
78-79	2.6616096084106864E-4	0.0011178760355324883	0.0	0.6542946180035709	7.097625622428497E-5
80-81	2.6616096084106864E-4	0.001126748067560524	0.0	0.6555633185835801	7.541227223830277E-5
82-83	2.6616096084106864E-4	0.001126748067560524	0.0	0.6559980481529538	7.984828825232058E-5
84-85	2.7503299286910424E-4	0.001126748067560524	0.0	0.6563440574020472	7.984828825232058E-5
86-87	2.7503299286910424E-4	0.0011533641636446307	0.0	0.656525934058622	7.984828825232058E-5
88-89	2.7503299286910424E-4	0.0012332124518969513	0.0	0.6565658582027482	7.984828825232058E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15125	0.0	52.34436	1
GTATCAA	27465	0.0	36.65808	1
TCAACGC	34610	0.0	28.728191	4
ATCAACG	34760	0.0	28.567263	3
CAACGCA	35460	0.0	28.0274	5
TATCAAC	35900	0.0	27.842857	2
AACGCAG	36355	0.0	27.420061	6
ACGCAGA	41540	0.0	23.956411	7
CGCAGAG	42730	0.0	23.279257	8
GTGGTAT	7435	0.0	21.009779	1
GCAGAGT	49325	0.0	20.062973	9
TGGTATC	7300	0.0	20.053612	2
GTACATG	34085	0.0	19.984394	1
GAGTACT	29080	0.0	19.804031	12-13
TACATGG	34460	0.0	19.333433	2
AGAGTAC	44805	0.0	18.754103	10-11
ACATGGG	34700	0.0	18.655554	3
CAGAGTA	48125	0.0	18.280567	10-11
GTACTTT	32235	0.0	17.832853	14-15
AGTACTT	31715	0.0	17.337835	12-13
>>END_MODULE