FastQCFastQC Report
Fri 27 May 2016
SRR2139085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9289039
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118850.051.238921
GTATCAA216750.037.540151
TCAACGC268250.029.833034
ATCAACG269400.029.65793
CAACGCA276600.028.9479485
TATCAAC278350.028.908582
AACGCAG284200.028.1442386
ACGCAGA329200.024.2579577
CGCAGAG334750.023.7916778
GTGGTAT60200.023.311381
TGGTATC58900.022.0043432
GTACATG272450.020.5875911
GCAGAGT386100.020.4943169
TACATGG270000.020.2657742
GAGACCG74200.019.90277388-89
GACCGAG75800.019.74126890-91
ACATGGG270750.019.620523
GAGTACT232500.019.6103512-13
AGACCGA75200.019.30889590-91
CGAGACC74750.019.25470588-89
Produced by FastQC (version 0.11.5)