##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139085_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9289039
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.573328629581596	34.0	31.0	34.0	31.0	34.0
2	32.70777375356052	34.0	31.0	34.0	31.0	34.0
3	32.69438765409425	34.0	31.0	34.0	31.0	34.0
4	36.224214797677135	37.0	35.0	37.0	35.0	37.0
5	36.182114748360945	37.0	35.0	37.0	35.0	37.0
6	36.22422943858886	37.0	37.0	37.0	35.0	37.0
7	36.183890820137584	37.0	37.0	37.0	35.0	37.0
8	36.15920710420098	37.0	37.0	37.0	35.0	37.0
9	37.89359319085645	39.0	38.0	39.0	35.0	39.0
10-11	37.82171551868821	39.0	38.0	39.0	35.0	39.0
12-13	37.794627086827816	39.0	38.0	39.0	35.0	39.0
14-15	39.145050634409	40.0	39.0	41.0	36.0	41.0
16-17	39.11147100362051	40.0	38.0	41.0	36.0	41.0
18-19	39.06624441990178	40.0	38.5	41.0	36.0	41.0
20-21	39.04797282783711	40.0	39.0	41.0	36.0	41.0
22-23	38.93355615976833	40.0	38.0	41.0	35.0	41.0
24-25	38.797394124684665	40.0	38.0	41.0	35.0	41.0
26-27	38.751875897596534	40.0	38.0	41.0	34.5	41.0
28-29	38.69825783779524	40.0	38.0	41.0	35.0	41.0
30-31	38.628795035805425	40.0	38.0	41.0	35.0	41.0
32-33	38.517249364100714	40.0	38.0	41.0	34.0	41.0
34-35	38.323338438896904	40.0	38.0	41.0	34.0	41.0
36-37	38.465735250500146	40.0	38.0	41.0	34.0	41.0
38-39	38.44860505835649	40.0	38.0	41.0	34.0	41.0
40-41	38.40961591221732	40.0	38.0	41.0	34.0	41.0
42-43	38.437833409611954	40.0	38.0	41.0	34.0	41.0
44-45	38.3719095534677	40.0	38.0	41.0	34.0	41.0
46-47	38.30873407545711	40.0	38.0	41.0	34.0	41.0
48-49	38.140593903938395	40.0	38.0	41.0	33.0	41.0
50-51	38.137623703624755	40.0	37.0	41.0	33.0	41.0
52-53	38.035408403893264	40.0	37.0	41.0	33.0	41.0
54-55	37.879237984249414	40.0	37.0	41.0	33.0	41.0
56-57	37.70669228658839	40.0	36.0	41.0	33.0	41.0
58-59	37.531851628932415	39.0	36.0	41.0	32.5	41.0
60-61	37.345786052845476	39.0	35.5	41.0	32.0	41.0
62-63	37.12342100190682	39.0	35.0	40.0	32.0	41.0
64-65	36.82567090100967	38.0	35.0	40.0	31.5	41.0
66-67	36.46342403514488	37.5	35.0	40.0	31.0	41.0
68-69	36.11599651929613	37.0	35.0	40.0	31.0	41.0
70-71	35.8112430467711	36.5	34.0	39.0	30.5	41.0
72-73	35.48146479404446	36.0	34.0	39.0	30.0	40.5
74-75	35.11530553318625	35.5	34.0	38.5	30.0	40.0
76-77	34.16010514128948	35.0	32.5	37.0	29.0	39.0
78-79	34.449014128201156	35.0	34.0	37.0	29.5	39.0
80-81	34.25744999975479	35.0	34.0	36.5	29.0	39.0
82-83	33.983739131405784	35.0	33.5	36.0	29.0	38.0
84-85	33.74516050398705	35.0	33.0	36.0	29.0	37.0
86-87	33.518867379208494	35.0	33.0	35.0	29.0	37.0
88-89	33.290349893878926	35.0	33.0	35.0	29.0	36.0
90-91	33.10773648570122	35.0	33.0	35.0	29.0	36.0
92-93	33.025390877202156	35.0	33.0	35.0	29.0	36.0
94-95	32.94862297197498	34.0	32.5	35.0	29.0	35.5
96-97	32.93471100104323	34.0	32.0	35.0	29.0	35.0
98-99	33.064390318030235	34.0	32.5	35.0	29.0	35.0
100-101	31.22083425665464	32.5	29.0	34.5	24.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	5.0
20	33.0
21	195.0
22	943.0
23	3470.0
24	9487.0
25	20607.0
26	37142.0
27	60487.0
28	89450.0
29	122742.0
30	160293.0
31	205585.0
32	262819.0
33	344539.0
34	479637.0
35	696209.0
36	1158710.0
37	2090228.0
38	2416956.0
39	1072763.0
40	56737.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.86193597227183	21.20859016832529	15.532803289210356	24.396670570192523
2	13.858070876272844	21.50222046888361	41.339245395889044	23.300463258954498
3	17.18040124284129	26.23633362249137	31.02640983921048	25.55685529545686
4	12.078393588132231	18.501834153745953	38.67086158127467	30.748910676847142
5	13.032910404715754	37.90338676620765	35.68186501120999	13.381837817866607
6	30.131893098079694	38.214743012139685	18.400764427920308	13.252599461860312
7	26.474054686825482	32.879863339992795	22.885394590914583	17.76068738226714
8	24.431633885916508	37.11519566232847	20.711571993615273	17.741598458139748
9	25.360632030934525	16.956393443928913	21.267883577623046	36.41509094751352
10-11	24.11912315236943	27.035004909826117	28.753936567404192	20.091935370400265
12-13	24.850311907160545	24.470517867296977	28.99295638639412	21.68621383914836
14-15	21.97300121616822	25.918808794606875	26.74069930587874	25.367490683346162
16-17	21.524577745569545	29.145620175908554	27.32833053096233	22.00147154755957
18-19	21.542556770404346	28.145629488690922	29.247062048076234	21.064751692828505
20-21	22.396652953897373	27.262460251136115	29.19589881887168	21.144987976094836
22-23	22.046938183796073	27.191665363031593	29.02367548433587	21.737720968836467
24-25	21.831419220909066	27.30347311131033	29.1779623521245	21.68714531565611
26-27	21.822696278963413	27.493940747934847	29.035526514597553	21.647836458504187
28-29	21.738266627014404	27.538783270558092	29.071760234492572	21.651189867934935
30-31	22.00604524198902	27.40094033370718	28.984587938437883	21.608426485865916
32-33	21.867642147437966	27.48837079224733	28.931218546289685	21.712768514025022
34-35	21.917748372455176	27.527833259262547	28.913690588447334	21.640727779834947
36-37	21.93355921197867	27.472815736055132	28.868937036826285	21.72468801513991
38-39	21.800604375637622	27.66373482622478	28.876597946723827	21.65906285141377
40-41	22.050699481212924	27.482673650830865	28.736124103360673	21.73050276459554
42-43	21.905676513253148	27.61924208204576	28.57242064891907	21.902660755782026
44-45	22.016345095606702	27.760473116384198	28.313496501663128	21.90968528634597
46-47	22.118330625410312	27.675622534257922	28.053489539078136	22.15255730125363
48-49	22.105854338190742	27.794585525595792	27.984988145412267	22.114571990801203
50-51	22.01902943951957	27.91496893138715	27.930547879912293	22.13545374918099
52-53	22.21875394431762	27.856221950318456	27.78899781048948	22.136026294874448
54-55	22.178920471636296	27.70891956952992	27.841601723226223	22.27055823560756
56-57	22.21011065671151	27.857414259135087	27.77464875648382	22.15782632766958
58-59	22.123271714632455	27.96497401329675	27.80982995575288	22.101924316317916
60-61	22.14932430168646	27.830798214175573	27.85205492815396	22.167822555984003
62-63	22.256445015257604	27.914323432611134	27.787322942109192	22.04190861002207
64-65	22.037156602730292	27.888305642420452	27.820898685182062	22.2536390696672
66-67	22.165117029442825	27.945048281134238	27.584426030403996	22.30540865901894
68-69	22.123141911646666	27.98889820314066	27.69956023157597	22.188399653636708
70-71	22.113544904621797	27.762722131407493	27.7636087504964	22.360124213474318
72-73	22.15195552653131	27.8138079577873	27.577154892008295	22.457081623673098
74-75	22.019062564679675	27.883017979503215	27.562892911736782	22.53502654408032
76-77	22.13050025470241	27.70839687586331	27.64385736026099	22.51724550917328
78-79	22.09035190669024	27.751612195934435	27.582890827011607	22.575145070363718
80-81	22.275129142446374	27.69852764588039	27.435428450420545	22.59091476125269
82-83	22.330645230527217	27.81346358447315	27.55290406900528	22.302987115994355
84-85	22.284623548746534	27.64357383268293	27.493560749719297	22.57824186885124
86-87	22.07002921599627	27.819723209541703	27.58894383507025	22.521303739391772
88-89	22.324007619247276	27.592406902982603	27.581135948903544	22.50244952886657
90-91	22.35402357335346	27.806632907117397	27.596885011423318	22.24245850810583
92-93	22.253649766317	27.680911622754934	27.631076922139147	22.434361688788922
94-95	22.237956623599526	27.535501967840194	27.720139449906796	22.50640195865349
96-97	22.09905472672946	27.74982693233695	27.85772837706475	22.293389963868837
98-99	22.289623937127885	28.194771980301013	27.96670898117427	21.54889510139683
100-101	20.8498994104001	31.83959005370926	25.680118492686	21.630392043204644
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1985.0
1	1711.5
2	2153.0
3	3018.0
4	4035.0
5	6144.0
6	8007.5
7	10003.0
8	11658.5
9	11984.0
10	11192.0
11	10428.0
12	11754.0
13	16264.5
14	27029.0
15	41552.5
16	53011.0
17	58776.0
18	59166.5
19	55936.0
20	50849.5
21	45337.0
22	42040.0
23	44048.5
24	51418.5
25	63488.0
26	79971.5
27	97861.0
28	116872.0
29	137498.5
30	156400.5
31	175666.5
32	197642.0
33	220960.0
34	242954.5
35	263317.0
36	285638.5
37	304646.5
38	317606.5
39	328058.5
40	341578.0
41	354887.0
42	361307.0
43	366628.0
44	372220.0
45	376996.5
46	378958.5
47	377479.0
48	375293.0
49	373027.0
50	368043.5
51	355777.5
52	336856.5
53	316710.0
54	297595.0
55	276266.0
56	253757.5
57	230211.0
58	205306.5
59	179251.0
60	151595.0
61	124771.0
62	99124.5
63	76231.0
64	57736.0
65	44408.5
66	34613.0
67	26003.0
68	18919.5
69	13950.5
70	9879.0
71	6641.5
72	4554.5
73	3042.5
74	2071.5
75	1356.0
76	846.0
77	524.5
78	342.0
79	222.5
80	155.0
81	105.0
82	77.0
83	51.5
84	31.5
85	22.0
86	15.5
87	12.5
88	10.0
89	8.0
90	6.0
91	3.5
92	2.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0765376267663426E-5
2	0.01465167710028992
3	1.0765376267663426E-5
4	1.5071526774728796E-4
5	1.0765376267663426E-5
6	1.0765376267663426E-5
7	1.0765376267663426E-5
8	0.0
9	0.0
10-11	1.4533257961345624E-4
12-13	6.136264472568153E-4
14-15	3.014305354945759E-4
16-17	0.0019216196637779214
18-19	0.0
20-21	0.0
22-23	2.637768069236136E-4
24-25	0.009039859264628178
26-27	0.019280311220844927
28-29	0.3408861196512381
30-31	0.3170240103111269
32-33	0.3108591608196645
34-35	0.18192652623424604
36-37	0.043099351236765414
38-39	0.03504775447856408
40-41	0.014007125162898799
42-43	0.012625852556267622
44-45	0.01617401905968715
46-47	0.010734089582872829
48-49	0.006258144603233348
50-51	0.0030618964951930226
52-53	0.001020108999511097
54-55	3.4807950901760884E-5
56-57	1.344068348797424E-4
58-59	8.303332881262988E-4
60-61	7.16247276262553E-4
62-63	8.356073791293927E-5
64-65	3.0100445185584296E-5
66-67	0.0032763425999121896
68-69	0.005101262203281186
70-71	0.006931268782389964
72-73	0.01080047755015867
74-75	0.008062077618268782
76-77	0.006055003575837291
78-79	0.0037563943416308986
80-81	0.004647626065061934
82-83	0.0015519474794556825
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0024520724331050956
92-93	0.01481850625204897
94-95	0.0012359608222102883
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	151.0
20-21	499.0
22-23	1126.0
24-25	2458.0
26-27	3469.0
28-29	5996.0
30-31	16423.0
32-33	21896.0
34-35	31579.0
36-37	44735.0
38-39	51905.0
40-41	56019.0
42-43	68047.0
44-45	81260.0
46-47	80604.0
48-49	70411.0
50-51	62687.0
52-53	55753.0
54-55	61288.0
56-57	65287.0
58-59	42996.0
60-61	70199.0
62-63	70183.0
64-65	77434.0
66-67	81901.0
68-69	79863.0
70-71	79678.0
72-73	78174.0
74-75	79213.0
76-77	79864.0
78-79	85888.0
80-81	88871.0
82-83	87988.0
84-85	91215.0
86-87	98731.0
88-89	108576.0
90-91	119941.0
92-93	131092.0
94-95	158446.0
96-97	245444.0
98-99	940224.0
100-101	5611525.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.44001775453548
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.88221294767115	76.7953787006596
2	7.063662624737033	12.070389201391613
3	1.7404931676421491	4.461233014349786
4	0.608877463183192	2.0809000505883373
5	0.2756826903814861	1.1777166980406137
6	0.1546518970973746	0.7928076500263367
7	0.08300041621608763	0.4964089924595452
8	0.04779533331047463	0.3266907301304714
9	0.036345075040227666	0.2794791471054278
>10	0.10550894454184098	1.329822079312657
>50	0.0013752644753399273	0.07828696865545372
>100	3.154230221891744E-4	0.051675066988505505
>500	5.90647702841146E-5	0.03514034151550355
>1k	1.9687911217893407E-5	0.024071358776133454
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.229612880299028E-5	0.0	0.0	0.0
3	0.0	3.229612880299028E-5	0.0	0.0	0.0
4	0.0	6.459225760598055E-5	0.0	1.0765376267663426E-5	0.0
5	0.0	7.535763387364398E-5	0.0	1.0765376267663426E-5	0.0
6	4.30615050706537E-5	7.535763387364398E-5	0.0	1.0765376267663426E-5	0.0
7	4.30615050706537E-5	7.535763387364398E-5	0.0	1.0765376267663426E-5	0.0
8	4.30615050706537E-5	8.61230101413074E-5	0.0	1.0765376267663426E-5	0.0
9	4.30615050706537E-5	1.722460202826148E-4	0.0	1.0765376267663426E-5	0.0
10-11	5.382688133831713E-5	1.8301139655027822E-4	0.0	2.153075253532685E-5	0.0
12-13	5.382688133831713E-5	1.8301139655027822E-4	0.0	2.6913440669158564E-5	5.382688133831713E-6
14-15	6.997494573981227E-5	2.3145558975476365E-4	0.0	3.767881693682199E-5	1.0765376267663426E-5
16-17	1.0765376267663426E-4	2.476036541562588E-4	0.0	7.535763387364398E-5	1.0765376267663426E-5
18-19	1.0765376267663426E-4	2.745170948254173E-4	0.0	8.074032200747569E-5	2.153075253532685E-5
20-21	1.0765376267663426E-4	3.014305354945759E-4	0.0	1.0765376267663426E-4	3.229612880299028E-5
22-23	1.0765376267663426E-4	3.2834397616373446E-4	0.0	3.014305354945759E-4	3.229612880299028E-5
24-25	1.3994989147962455E-4	3.8755354563588327E-4	0.0	8.558474132792423E-4	3.229612880299028E-5
26-27	1.5071526774728796E-4	4.0908429817121015E-4	0.0	0.0025029499822317464	3.229612880299028E-5
28-29	1.6148064401495137E-4	4.1446698630504187E-4	0.0	0.015066144086594964	3.229612880299028E-5
30-31	1.6148064401495137E-4	4.68293867643359E-4	0.0	0.021385419955713393	3.229612880299028E-5
32-33	1.6148064401495137E-4	4.7367655577719073E-4	0.0	0.023716123917662528	3.229612880299028E-5
34-35	1.7762870841644653E-4	5.275034371155079E-4	0.0	0.02667660239126997	3.229612880299028E-5
36-37	1.8301139655027822E-4	5.81330318453825E-4	0.0	0.031951636762425045	3.229612880299028E-5
38-39	1.8301139655027822E-4	5.81330318453825E-4	0.0	0.041855782928675395	4.30615050706537E-5
40-41	1.8301139655027822E-4	6.782187048627958E-4	0.0	0.061992419237339835	4.30615050706537E-5
42-43	1.8839408468410994E-4	7.051321455319544E-4	0.0	0.07013642638382722	4.30615050706537E-5
44-45	1.9915946095177336E-4	8.558474132792423E-4	0.0	0.08261888016618296	4.30615050706537E-5
46-47	2.0454214908560508E-4	9.581184878220449E-4	0.0	0.09915988080144782	4.30615050706537E-5
48-49	2.0454214908560508E-4	9.635011759558767E-4	0.0	0.11286958747831718	4.30615050706537E-5
50-51	2.099248372194368E-4	0.001065772250498679	0.0	0.1303202624081996	5.382688133831713E-5
52-53	2.3683827788859537E-4	0.0010765376267663426	0.0	0.1497894453882689	5.382688133831713E-5
54-55	2.3683827788859537E-4	0.0010765376267663426	0.0	0.21172265505613658	5.382688133831713E-5
56-57	2.3683827788859537E-4	0.001087303003034006	0.0	0.2637947800628246	5.382688133831713E-5
58-59	2.3683827788859537E-4	0.001087303003034006	0.0	0.3058228090117826	5.382688133831713E-5
60-61	2.3683827788859537E-4	0.0011142164437031644	0.0	0.590572393979614	5.382688133831713E-5
62-63	2.476036541562588E-4	0.0011303645081046597	0.0	0.5961811550150666	5.382688133831713E-5
64-65	2.476036541562588E-4	0.001216487518245967	0.0	0.6020644331453447	5.382688133831713E-5
66-67	2.476036541562588E-4	0.001216487518245967	0.0	0.607172604184351	5.382688133831713E-5
68-69	2.583690304239222E-4	0.0012541663351827892	0.0	0.6121785041488146	5.382688133831713E-5
70-71	2.583690304239222E-4	0.0012756970877181159	0.0	0.616926035082854	5.382688133831713E-5
72-73	2.7989978295924904E-4	0.0012864624639857794	0.0	0.6209415204306925	5.382688133831713E-5
74-75	2.7989978295924904E-4	0.0013295239690564332	0.0	0.6242949351380698	5.382688133831713E-5
76-77	2.906651592269125E-4	0.0014156469791977405	0.0	0.6270185753337886	5.382688133831713E-5
78-79	3.014305354945759E-4	0.0014425604198668991	0.0	0.6296022656380278	5.382688133831713E-5
80-81	3.0681322362840764E-4	0.0014425604198668991	0.0	0.6308564319732106	5.920956947214884E-5
82-83	3.175785998960711E-4	0.0014533257961345625	0.0	0.6313785527221922	6.459225760598055E-5
84-85	3.2296128802990274E-4	0.0014748565486698891	0.0	0.6317230447627575	6.459225760598055E-5
86-87	3.2296128802990274E-4	0.0015071526774728795	0.0	0.6318468465898356	6.459225760598055E-5
88-89	3.2296128802990274E-4	0.0015609795588111966	0.0	0.6319706484169137	6.997494573981227E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11885	0.0	51.23892	1
GTATCAA	21675	0.0	37.54015	1
TCAACGC	26825	0.0	29.83303	4
ATCAACG	26940	0.0	29.6579	3
CAACGCA	27660	0.0	28.947948	5
TATCAAC	27835	0.0	28.90858	2
AACGCAG	28420	0.0	28.144238	6
ACGCAGA	32920	0.0	24.257957	7
CGCAGAG	33475	0.0	23.791677	8
GTGGTAT	6020	0.0	23.31138	1
TGGTATC	5890	0.0	22.004343	2
GTACATG	27245	0.0	20.587591	1
GCAGAGT	38610	0.0	20.494316	9
TACATGG	27000	0.0	20.265774	2
GAGACCG	7420	0.0	19.902773	88-89
GACCGAG	7580	0.0	19.741268	90-91
ACATGGG	27075	0.0	19.62052	3
GAGTACT	23250	0.0	19.61035	12-13
AGACCGA	7520	0.0	19.308895	90-91
CGAGACC	7475	0.0	19.254705	88-89
>>END_MODULE