FastQCFastQC Report
Fri 27 May 2016
SRR2139084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12646922
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156500.050.200811
GTATCAA286750.037.672491
TCAACGC351900.030.1072224
ATCAACG352200.030.0085333
CAACGCA358750.029.5562555
TATCAAC370250.028.817412
AACGCAG369750.028.7468786
TATCTCG167000.026.84112594-95
ACGCAGA423300.025.0899687
CGCAGAG437500.024.2266168
CTATCTC264050.020.9908694-95
GCAGAGT504750.020.8458989
GTACATG368900.020.2506561
TCGTATC13450.019.91316694-95
GTGGTAT84200.019.8633331
TACATGG369650.019.8151232
TGGTATC78950.019.554652
ACATGGG372200.019.1226863
GAGTACT296950.019.11735712-13
AGAGTAC458300.018.94995510-11
Produced by FastQC (version 0.11.5)