##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139084_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12646922
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60667915877081	34.0	31.0	34.0	31.0	34.0
2	32.73907153060642	34.0	31.0	34.0	31.0	34.0
3	32.72551044435951	34.0	31.0	34.0	31.0	34.0
4	36.23730572545636	37.0	37.0	37.0	35.0	37.0
5	36.19411576982921	37.0	37.0	37.0	35.0	37.0
6	36.23574574113765	37.0	37.0	37.0	35.0	37.0
7	36.193914535093995	37.0	37.0	37.0	35.0	37.0
8	36.17018805049956	37.0	37.0	37.0	35.0	37.0
9	37.9087740084109	39.0	38.0	39.0	35.0	39.0
10-11	37.8393031126467	39.0	38.0	39.0	35.0	39.0
12-13	37.81458120798088	39.0	38.0	39.0	35.0	39.0
14-15	39.17814615287419	41.0	39.0	41.0	36.0	41.0
16-17	39.14921140495687	40.0	39.0	41.0	36.0	41.0
18-19	39.10598464195478	40.0	38.5	41.0	36.0	41.0
20-21	39.08954989748209	40.0	39.0	41.0	36.0	41.0
22-23	38.98147932994336	40.0	38.5	41.0	35.0	41.0
24-25	38.85263248554678	40.0	38.0	41.0	35.0	41.0
26-27	38.805679475941446	40.0	38.0	41.0	35.0	41.0
28-29	38.74262489460086	40.0	38.0	41.0	35.0	41.0
30-31	38.67650633888702	40.0	38.0	41.0	35.0	41.0
32-33	38.56770852711186	40.0	38.0	41.0	34.5	41.0
34-35	38.37809165234604	40.0	38.0	41.0	34.0	41.0
36-37	38.525501880537234	40.0	38.0	41.0	34.0	41.0
38-39	38.5118011884216	40.0	38.0	41.0	34.5	41.0
40-41	38.480617344120546	40.0	38.0	41.0	34.0	41.0
42-43	38.5154576418824	40.0	38.0	41.0	34.0	41.0
44-45	38.45340721595302	40.0	38.0	41.0	34.0	41.0
46-47	38.39662218407629	40.0	38.0	41.0	34.0	41.0
48-49	38.22980467385565	40.0	38.0	41.0	33.0	41.0
50-51	38.22530346727362	40.0	37.5	41.0	33.0	41.0
52-53	38.1288532418669	40.0	37.0	41.0	33.0	41.0
54-55	37.98635579495294	40.0	37.0	41.0	33.0	41.0
56-57	37.81721211351068	40.0	36.5	41.0	33.0	41.0
58-59	37.651834241458474	39.0	36.0	41.0	33.0	41.0
60-61	37.4718486630982	39.0	36.0	41.0	32.5	41.0
62-63	37.26398496170249	39.0	35.0	41.0	32.0	41.0
64-65	36.98591782322708	38.5	35.0	40.0	32.0	41.0
66-67	36.63774025206382	38.0	35.0	40.0	31.0	41.0
68-69	36.300630262123434	37.0	35.0	40.0	31.0	41.0
70-71	36.00169137684492	37.0	35.0	39.5	31.0	41.0
72-73	35.68183524481781	36.0	34.0	39.0	31.0	41.0
74-75	35.3115711581771	36.0	34.0	39.0	30.0	40.0
76-77	34.35170957596635	35.0	33.0	37.0	29.0	39.0
78-79	34.59871225806087	35.0	34.0	37.0	30.0	39.0
80-81	34.38543268679174	35.0	34.0	37.0	30.0	39.0
82-83	34.11091147393399	35.0	34.0	36.0	30.0	38.0
84-85	33.87975230602595	35.0	33.5	36.0	30.0	37.0
86-87	33.66015930303908	35.0	33.0	35.0	29.0	37.0
88-89	33.436067442043324	35.0	33.0	35.0	29.0	36.0
90-91	33.273421618269126	35.0	33.0	35.0	29.0	36.0
92-93	33.19755439671131	35.0	33.0	35.0	29.0	36.0
94-95	33.12192847370531	35.0	33.0	35.0	29.0	35.5
96-97	33.08802647955024	34.5	33.0	35.0	29.0	35.0
98-99	33.189733114656704	34.0	33.0	35.0	29.0	35.0
100-101	31.336821101488447	32.5	29.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	2.0
19	11.0
20	66.0
21	354.0
22	1611.0
23	5371.0
24	14185.0
25	29762.0
26	53054.0
27	84521.0
28	121175.0
29	162798.0
30	209883.0
31	264432.0
32	335108.0
33	437479.0
34	607710.0
35	886298.0
36	1495810.0
37	2786318.0
38	3458209.0
39	1589505.0
40	103259.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.02111826269809	21.309803390089968	15.321523713163069	24.347554634048873
2	13.86838927969008	21.528505622974023	41.399486793842314	23.20361830349358
3	16.981405988066186	26.56156387787984	31.2132020117782	25.24382812227577
4	12.100498232413944	18.58768810624003	38.85131141530314	30.460502246042886
5	12.990047142699792	37.98220135952458	35.83939521722323	13.188356280552396
6	29.864225450605726	38.29050564955692	18.60429111560039	13.240977784236971
7	26.249495825901025	33.00178754971269	23.207996634121457	17.540719990264826
8	24.384288920260598	36.8757156879753	21.05993062976114	17.68006476200296
9	25.44491062726567	16.84165522646538	21.496613958716594	36.21682018755236
10-11	23.993767804254432	26.979442483446743	29.000810237633594	20.025979474665228
12-13	24.797024362927715	24.49436372949507	29.111826213002807	21.596785694574407
14-15	21.98304271321649	25.924575865211487	26.76354078572734	25.328840635844685
16-17	21.58821817036855	29.140416889172105	27.37554465318981	21.895820287269533
18-19	21.59756513067489	28.185366224711927	29.197023528493617	21.02004511611957
20-21	22.429531425647085	27.261741876227585	29.098659107709405	21.210067590415925
22-23	22.077274916314966	27.268479308662357	28.88347595177148	21.770769823251193
24-25	21.876067594201608	27.436337471732852	28.98732171695923	21.700273217106314
26-27	21.907700060251557	27.55557521560862	28.883867222472897	21.65285750166693
28-29	21.84178183877861	27.54895418752717	28.939967263926196	21.669296709768027
30-31	22.0341717015172	27.361560772991478	28.85247303953261	21.751794485958712
32-33	21.789431091843667	27.581711188238785	28.764416970515732	21.864440749401812
34-35	22.00568954674991	27.626069523801277	28.745816160536513	21.6224247689123
36-37	22.003701690637286	27.52321843477647	28.731101875677783	21.74197799890846
38-39	21.88813287872037	27.69727558042675	28.714939202248736	21.699652338604146
40-41	22.044899192887602	27.46529400506514	28.661930085866206	21.827876716181052
42-43	22.065416381488756	27.567259524194842	28.429547842016806	21.9377762522996
44-45	21.96469817608957	27.73662556518395	28.24623459208552	22.05244166664096
46-47	22.12554705128127	27.576536079782837	28.03643658679468	22.261480282141218
48-49	22.118429339861915	27.813854025948643	27.894022534179737	22.173694100009712
50-51	21.983720611593483	27.86173599446528	27.939872491656477	22.214670902284762
52-53	22.131879768949492	27.808212414602103	27.70346843911514	22.356439377333263
54-55	22.10167688073582	27.496474526213838	27.881181728582	22.52066686446834
56-57	22.230391773836526	27.364532253176282	27.922663494717277	22.482412478269918
58-59	21.87482758765219	27.35599842712893	28.254219178745405	22.514954806473476
60-61	22.038614406315276	27.18545761191053	28.371074314567224	22.40485366720697
62-63	21.833620531565156	27.636040486390435	28.29628073774888	22.234058244295525
64-65	21.37479532274372	27.911601846071495	28.198920719398956	22.514682111785834
66-67	21.58642914570861	28.288595494543706	27.690717767781404	22.43425759196628
68-69	21.62198262121778	28.475034808840928	27.40611742744109	22.496865142500205
70-71	21.583915488189017	28.12020812041758	27.35309028317104	22.942786108222364
72-73	21.55601861403388	27.744240423629734	27.337890562318044	23.361850400018337
74-75	21.907513317283218	27.428181433927595	27.361262952126857	23.303042296662323
76-77	22.28857084944408	27.294667163038568	27.230604047644576	23.18615793987278
78-79	22.426947335808443	27.209216113685642	26.659119871641206	23.704716678864713
80-81	22.45323754216362	27.597663052291878	26.504468245479103	23.444631160065402
82-83	22.299807903142327	27.669824756468536	26.708756246726228	23.321611093662906
84-85	22.43858545805011	27.645602898768452	26.598455495433893	23.317356147747546
86-87	22.567313438925865	28.000482438981066	26.598444154287666	22.8337599678054
88-89	22.785724183121317	27.599807736523918	26.900380821769016	22.714087258585746
90-91	22.486214036561044	27.612828245208053	27.136754693933558	22.76420302429734
92-93	22.37476859954507	27.200978416837035	27.05591302924108	23.368339954376815
94-95	22.143809535796528	27.175051811591388	27.708199045418798	22.97293960719329
96-97	21.726829301493275	27.40247020483241	27.984563717204786	22.886136776469527
98-99	21.908247978989806	27.542427278249193	28.280624824865576	22.268699917895425
100-101	20.76710712129045	31.260357416172486	26.05929512024537	21.913240342291694
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2484.0
1	2225.0
2	2995.5
3	4227.5
4	5715.0
5	8810.0
6	11407.5
7	13822.5
8	15708.5
9	15989.5
10	15119.0
11	14290.0
12	16046.5
13	21768.0
14	35049.0
15	53561.5
16	68849.5
17	77124.0
18	78293.5
19	74237.0
20	67626.5
21	60942.0
22	57632.0
23	60650.5
24	69687.5
25	85388.5
26	107243.5
27	131379.5
28	157823.0
29	186421.0
30	213620.0
31	240518.0
32	268235.0
33	296441.5
34	325229.0
35	352129.0
36	382259.5
37	409280.0
38	428626.0
39	446324.0
40	464577.0
41	481074.5
42	489431.0
43	496194.0
44	503828.0
45	512934.5
46	518739.0
47	521126.0
48	527470.0
49	524113.0
50	507731.0
51	488912.5
52	464241.5
53	435913.0
54	409117.5
55	379604.0
56	348112.5
57	313811.0
58	276640.5
59	239117.0
60	201173.0
61	166403.5
62	133420.5
63	103986.5
64	81042.5
65	65413.0
66	54592.5
67	40513.0
68	28044.5
69	21722.5
70	15730.0
71	9735.0
72	6386.0
73	4288.0
74	2767.0
75	1792.5
76	1173.0
77	709.5
78	443.5
79	301.0
80	196.5
81	135.5
82	110.5
83	76.5
84	49.5
85	34.5
86	25.0
87	18.0
88	13.0
89	10.0
90	8.5
91	8.0
92	6.0
93	2.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.907062287566888E-6
2	0.01661273786617803
3	7.907062287566888E-6
4	1.0279180973836954E-4
5	7.907062287566888E-6
6	7.907062287566888E-6
7	7.907062287566888E-6
8	0.0
9	0.0
10-11	1.1860593431350331E-4
12-13	6.641932321556186E-4
14-15	2.2535127519565632E-4
16-17	0.001798856670421467
18-19	7.907062287566888E-6
20-21	3.953649938457485E-6
22-23	2.4118945783812556E-4
24-25	0.009356578987955085
26-27	0.02009959445972737
28-29	0.34959773860097104
30-31	0.32526691510979644
32-33	0.3188972364714779
34-35	0.187222435270731
36-37	0.04642871433716138
38-39	0.03773502564033194
40-41	0.016004540175724672
42-43	0.014664723977940474
44-45	0.017942816412705013
46-47	0.012669432369136915
48-49	0.007730176815106753
50-51	0.004072218576395518
52-53	0.0015407669921061655
54-55	9.804814701151594E-5
56-57	2.7326648495014724E-4
58-59	0.001261668239332563
60-61	0.0011075271969830272
62-63	2.68726149199792E-4
64-65	7.88230147438449E-5
66-67	0.0033930414124690995
68-69	0.005656228758328707
70-71	0.0073305198100157756
72-73	0.01152011782517962
74-75	0.008488107239238252
76-77	0.006494996484495225
78-79	0.0038488691917906233
80-81	0.004782216192411003
82-83	0.0016942132927877582
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0026616904746970213
92-93	0.01556167732200544
94-95	0.001392513366119882
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	237.0
20-21	728.0
22-23	1505.0
24-25	3804.0
26-27	5296.0
28-29	9302.0
30-31	27004.0
32-33	35443.0
34-35	51263.0
36-37	71281.0
38-39	60993.0
40-41	88288.0
42-43	103842.0
44-45	121085.0
46-47	118175.0
48-49	100174.0
50-51	96456.0
52-53	18463.0
54-55	18534.0
56-57	19131.0
58-59	20745.0
60-61	111106.0
62-63	115710.0
64-65	126080.0
66-67	134423.0
68-69	127342.0
70-71	123418.0
72-73	121065.0
74-75	123148.0
76-77	124689.0
78-79	131345.0
80-81	133662.0
82-83	131972.0
84-85	136468.0
86-87	145389.0
88-89	155591.0
90-91	167132.0
92-93	179971.0
94-95	211997.0
96-97	319052.0
98-99	1290892.0
100-101	7564721.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.21830682617411
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.07419072914297	72.41310836835657
2	7.921260261175565	13.025452132066254
3	2.135942575714501	5.268407461595504
4	0.8341950004361113	2.743444019948665
5	0.4045603740788382	1.6631134482862848
6	0.20902932462042645	1.0311622288386104
7	0.1298717694093384	0.7474485889748581
8	0.08114587225141269	0.5337340977955342
9	0.048089172222719474	0.35584292851418514
>10	0.1580659219204769	1.9409921110678037
>50	0.00299198713806021	0.15927871175086722
>100	6.062433397950814E-4	0.08099171776642147
>500	3.38460145280924E-5	0.020954182706401113
>1k	1.6922535223137374E-5	0.016070002332068078
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.3721186862700662E-5	0.0	1.5814124575133776E-5	7.907062287566888E-6
3	0.0	2.3721186862700662E-5	0.0	1.5814124575133776E-5	7.907062287566888E-6
4	0.0	4.7442373725401324E-5	0.0	1.5814124575133776E-5	7.907062287566888E-6
5	0.0	4.7442373725401324E-5	0.0	1.5814124575133776E-5	7.907062287566888E-6
6	0.0	4.7442373725401324E-5	0.0	2.3721186862700662E-5	1.5814124575133776E-5
7	0.0	4.7442373725401324E-5	0.0	2.3721186862700662E-5	1.5814124575133776E-5
8	0.0	5.534943601296822E-5	0.0	2.3721186862700662E-5	1.5814124575133776E-5
9	0.0	9.488474745080265E-5	0.0	2.3721186862700662E-5	1.5814124575133776E-5
10-11	0.0	9.488474745080265E-5	0.0	3.162824915026755E-5	1.5814124575133776E-5
12-13	0.0	9.883827859458609E-5	0.0	3.162824915026755E-5	1.5814124575133776E-5
14-15	0.0	1.5023418346377088E-4	0.0	3.162824915026755E-5	2.767471800648411E-5
16-17	0.0	1.5814124575133775E-4	0.0	5.139590486918477E-5	3.5581780294050995E-5
18-19	0.0	1.7395537032647153E-4	0.0	7.907062287566888E-5	3.953531143783444E-5
20-21	0.0	1.897694949016053E-4	0.0	1.2255946545728676E-4	4.348884258161788E-5
22-23	0.0	2.411653997707901E-4	0.0	3.3605014722159273E-4	6.32564983005351E-5
24-25	0.0	2.965148357837583E-4	0.0	0.0012493158414355683	7.907062287566888E-5
26-27	0.0	3.162824915026755E-4	0.0	0.003554224498261316	8.697768516323576E-5
28-29	0.0	3.162824915026755E-4	0.0	0.020419988357641486	8.697768516323576E-5
30-31	0.0	3.7163192751564375E-4	0.0	0.02944589995889909	8.697768516323576E-5
32-33	0.0	3.874460520907775E-4	0.0	0.03429688267232138	1.0279180973836954E-4
34-35	0.0	4.4674901924752916E-4	0.0	0.039875315116199815	1.1860593431350332E-4
36-37	0.0	5.060519864042808E-4	0.0	0.04942704635958062	1.2255946545728676E-4
38-39	0.0	5.139590486918477E-4	0.0	0.07180403263339491	1.265129966010702E-4
40-41	0.0	5.574478912734656E-4	0.0	0.28258259203306546	1.3837359003242053E-4
42-43	0.0	6.048902649988669E-4	0.0	0.3048053905922722	1.4232712117620398E-4
44-45	0.0	6.879144190183193E-4	0.0	0.34352627461448726	1.5023418346377088E-4
46-47	0.0	7.511709173188543E-4	0.0	0.405857646627377	1.5023418346377088E-4
48-49	0.0	7.788456353253385E-4	0.0	0.45226024166196327	1.7395537032647153E-4
50-51	0.0	8.737303827761412E-4	0.0	0.5467298683426687	1.7395537032647153E-4
52-53	0.0	8.776839139199245E-4	0.0	0.7324430402907521	1.8581596375782185E-4
54-55	0.0	8.81637445063708E-4	0.0	1.392338784093078	1.976765571891722E-4
56-57	0.0	8.934980384950583E-4	0.0	2.1175152341415564	1.976765571891722E-4
58-59	0.0	9.053586319264086E-4	0.0	2.8603481542781712	1.976765571891722E-4
60-61	0.0	9.409404122204596E-4	0.0	3.411699700527923	1.976765571891722E-4
62-63	0.0	9.567545367955934E-4	0.0	3.42215679040323	2.2139774405187287E-4
64-65	0.0	0.001051639284246396	0.0	3.4288145368493614	2.253512751956563E-4
66-67	0.0	0.001051639284246396	0.0	3.4346064599750044	2.411653997707901E-4
68-69	0.0	0.0010714069399653133	0.0	3.4401690782943075	2.49072462058357E-4
70-71	0.0	0.0010793140022528802	0.0	3.4450991316306054	2.530259932021404E-4
72-73	0.0	0.001087221064540447	0.0	3.4493096422987346	2.8465424235240796E-4
74-75	0.0	0.0011386169694096318	0.0	3.4527927032364083	2.8465424235240796E-4
76-77	0.0	0.0012058269988539504	0.0	3.456394370108395	2.8465424235240796E-4
78-79	0.0	0.0012255946545728677	0.0	3.4598813845772116	2.8465424235240796E-4
80-81	0.0	0.001229548185716651	0.0	3.4613402375692677	2.965148357837583E-4
82-83	0.0	0.0012414087791480014	0.0	3.461905592522829	3.083754292151086E-4
84-85	0.0	0.0012690834971544854	0.0	3.4622732709192006	3.162824915026755E-4
86-87	0.0	0.001280944090585836	0.0	3.4624551333518148	3.162824915026755E-4
88-89	0.0	0.001344200588886371	0.0	3.4625262969124027	3.162824915026755E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15650	0.0	50.20081	1
GTATCAA	28675	0.0	37.67249	1
TCAACGC	35190	0.0	30.107222	4
ATCAACG	35220	0.0	30.008533	3
CAACGCA	35875	0.0	29.556255	5
TATCAAC	37025	0.0	28.81741	2
AACGCAG	36975	0.0	28.746878	6
TATCTCG	16700	0.0	26.841125	94-95
ACGCAGA	42330	0.0	25.089968	7
CGCAGAG	43750	0.0	24.226616	8
CTATCTC	26405	0.0	20.99086	94-95
GCAGAGT	50475	0.0	20.845898	9
GTACATG	36890	0.0	20.250656	1
TCGTATC	1345	0.0	19.913166	94-95
GTGGTAT	8420	0.0	19.863333	1
TACATGG	36965	0.0	19.815123	2
TGGTATC	7895	0.0	19.55465	2
ACATGGG	37220	0.0	19.122686	3
GAGTACT	29695	0.0	19.117357	12-13
AGAGTAC	45830	0.0	18.949955	10-11
>>END_MODULE