FastQCFastQC Report
Fri 27 May 2016
SRR2139083_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139083_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7591603
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90400.048.915521
GTATCAA167800.037.5877761
TCAACGC202850.030.4506324
ATCAACG204550.030.1342963
CAACGCA208950.029.5616685
TATCAAC211850.029.3241372
AACGCAG214650.028.8979956
TATCTCG91600.027.9340694-95
ACGCAGA248700.024.9415157
CGCAGAG254000.024.4380658
CTATCTC145950.021.10350694-95
GCAGAGT293900.020.973579
GTACATG210650.020.4799921
TACATGG214700.019.7118872
GAGTACT171550.019.31175212-13
ACATGGG214500.019.0034663
AGAGTAC265850.018.99285310-11
CGCTATC144550.018.45873392-93
CAGAGTA289300.018.07959710-11
GTGGTAT53250.017.904571
Produced by FastQC (version 0.11.5)