##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139083_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7591603
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61934719189083	34.0	31.0	34.0	31.0	34.0
2	32.75356811993462	34.0	31.0	34.0	31.0	34.0
3	32.74126571160267	34.0	31.0	34.0	31.0	34.0
4	36.24577879006581	37.0	37.0	37.0	35.0	37.0
5	36.203915826473015	37.0	37.0	37.0	35.0	37.0
6	36.244652545714	37.0	37.0	37.0	35.0	37.0
7	36.205460954689016	37.0	37.0	37.0	35.0	37.0
8	36.18431574991474	37.0	37.0	37.0	35.0	37.0
9	37.927592630963446	39.0	38.0	39.0	35.0	39.0
10-11	37.86019158272634	39.0	38.0	39.0	35.0	39.0
12-13	37.8321727967071	39.0	38.0	39.0	35.0	39.0
14-15	39.20295476462613	41.0	39.0	41.0	36.0	41.0
16-17	39.17408233807801	40.0	39.0	41.0	36.0	41.0
18-19	39.12844691694231	40.0	39.0	41.0	36.0	41.0
20-21	39.11153088965464	40.0	39.0	41.0	36.0	41.0
22-23	39.000065701773266	40.0	38.5	41.0	35.0	41.0
24-25	38.87017762811443	40.0	38.0	41.0	35.0	41.0
26-27	38.821602469824015	40.0	38.0	41.0	35.0	41.0
28-29	38.76019273379143	40.0	38.0	41.0	35.0	41.0
30-31	38.69877892604573	40.0	38.0	41.0	35.0	41.0
32-33	38.59328595558496	40.0	38.0	41.0	35.0	41.0
34-35	38.40762194485053	40.0	38.0	41.0	34.0	41.0
36-37	38.550733378608555	40.0	38.0	41.0	34.0	41.0
38-39	38.540022225264025	40.0	38.0	41.0	34.5	41.0
40-41	38.50530535059494	40.0	38.0	41.0	34.0	41.0
42-43	38.53763344056273	40.0	38.0	41.0	34.0	41.0
44-45	38.47475103193188	40.0	38.0	41.0	34.0	41.0
46-47	38.41608693202001	40.0	38.0	41.0	34.0	41.0
48-49	38.25415464245033	40.0	38.0	41.0	33.0	41.0
50-51	38.24991986688977	40.0	38.0	41.0	33.0	41.0
52-53	38.154520949120545	40.0	37.0	41.0	33.0	41.0
54-55	38.008389490738224	40.0	37.0	41.0	33.0	41.0
56-57	37.83976958737689	40.0	37.0	41.0	33.0	41.0
58-59	37.676405428021795	39.5	36.0	41.0	33.0	41.0
60-61	37.49578372995799	39.0	36.0	41.0	33.0	41.0
62-63	37.28342468013659	39.0	35.0	41.0	32.0	41.0
64-65	36.998996039977804	38.5	35.0	40.0	32.0	41.0
66-67	36.64730150888399	38.0	35.0	40.0	31.0	41.0
68-69	36.31147799080743	37.0	35.0	40.0	31.0	41.0
70-71	36.00618102571957	37.0	35.0	39.5	31.0	41.0
72-73	35.6822947527542	36.0	34.0	39.0	31.0	41.0
74-75	35.308308673672485	36.0	34.0	39.0	30.0	40.0
76-77	34.34434195266644	35.0	33.0	37.0	29.0	39.0
78-79	34.59641059600399	35.0	34.0	37.0	30.0	39.0
80-81	34.38063707689912	35.0	34.0	37.0	30.0	39.0
82-83	34.1053387746811	35.0	34.0	36.0	30.0	38.5
84-85	33.87328179719056	35.0	33.5	36.0	29.0	37.0
86-87	33.64968937497028	35.0	33.0	35.0	29.0	37.0
88-89	33.42243747297833	35.0	33.0	35.0	29.0	36.5
90-91	33.253790797569195	35.0	33.0	35.0	29.0	36.0
92-93	33.17716302765155	35.0	33.0	35.0	29.0	36.0
94-95	33.10033728050662	35.0	33.0	35.0	29.0	35.5
96-97	33.06620943492371	34.0	33.0	35.0	29.0	35.0
98-99	33.173117280096314	34.0	33.0	35.0	29.0	35.0
100-101	31.2981796712085	32.5	29.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	1.0
19	6.0
20	32.0
21	191.0
22	968.0
23	3345.0
24	8659.0
25	18045.0
26	32192.0
27	50293.0
28	71872.0
29	97464.0
30	125059.0
31	158384.0
32	199579.0
33	261164.0
34	365627.0
35	532220.0
36	897257.0
37	1689918.0
38	2098224.0
39	924169.0
40	56932.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.14418052265919	21.080296501357225	15.315280663459527	24.460242312524063
2	13.774788491781752	21.45423271364876	41.365562429326545	23.405416365242946
3	17.133434699742516	26.332232160251834	31.093046117676625	25.441287022329018
4	12.189200442753158	18.466476517630234	38.61041476296991	30.733908276646694
5	13.103797209574106	37.87554166769302	35.716366020816956	13.304295101915919
6	30.09441222024021	38.030602763422	18.561997849729213	13.312987166608576
7	26.312390981508248	32.87294302309315	23.11373804896516	17.70092794643344
8	24.42062631568063	36.970900612163206	20.97510894602892	17.633364126127248
9	25.44122763005389	16.835034708743333	21.298215936739577	36.4255217244632
10-11	24.074402044103394	26.87600131329494	28.95221918194371	20.09737746065796
12-13	24.830856536243513	24.401978855856804	29.13962264797143	21.627541959928255
14-15	21.95112071853812	25.870620748867772	26.70744254111512	25.47081599147898
16-17	21.581810067451986	29.04011131435081	27.3868421336884	21.991236484508807
18-19	21.65136928261396	28.1835865231625	29.12846601699272	21.036578177230815
20-21	22.35403405732583	27.27010527866025	29.062204393221414	21.313656270792507
22-23	22.098738889134804	27.21569148207401	28.909169665045752	21.776399963745437
24-25	21.898732467446898	27.382425854699093	28.940902838976417	21.777938838877585
26-27	21.880748465065484	27.524736733236416	28.90044622557918	21.694068576118923
28-29	21.79906711960192	27.545909717192053	28.903630303943423	21.751392859262605
30-31	22.03603013559561	27.33732814627942	28.87672954683696	21.749912171288006
32-33	21.819697523818775	27.541061587221193	28.796840961533395	21.842399927426634
34-35	21.978715650187226	27.545433680707898	28.78969163807845	21.686159031026424
36-37	21.902314172976432	27.54076463687747	28.79747653833849	21.759444651807613
38-39	21.896245025248568	27.6097079213873	28.717781820146122	21.77626523321801
40-41	22.03849281648143	27.39494317396608	28.66225625442468	21.904307755127814
42-43	22.00350309085886	27.561443714810828	28.471661727383562	21.963391466946746
44-45	21.967169374485454	27.6337348462181	28.311121250348386	22.087974528948063
46-47	22.065693438209053	27.596044777843804	28.061246555079585	22.277015228867562
48-49	22.03742195060296	27.806576695260187	28.002486667431338	22.153514686705517
50-51	21.98550022024085	27.883354609373516	27.9304604145381	22.200684755847533
52-53	22.09875388181612	27.78837058903133	27.734296451300043	22.378579077852507
54-55	22.059069707263173	27.551057109724216	27.874694214167068	22.515178968845547
56-57	22.120205001084287	27.391249942671493	28.037448397264765	22.451096658979456
58-59	21.908665733781092	27.33732107769622	28.22684701383158	22.527166174691107
60-61	21.994033696086433	27.183319503869402	28.394749242794166	22.427897557249995
62-63	21.790358782777503	27.665727419432518	28.31459683798807	22.229316959801906
64-65	21.45201684625201	27.84513818221973	28.17299724687654	22.529847724651713
66-67	21.543641814092577	28.21454195511238	27.811370891456345	22.430445339338696
68-69	21.611736713416477	28.429157835787922	27.495555632204567	22.463549818591037
70-71	21.586589900370228	28.090041787996327	27.449498971177334	22.87386934045611
72-73	21.54046302677993	27.797503225017394	27.39570075285429	23.266332995348385
74-75	21.853746348333207	27.484113000999287	27.464368584640038	23.19777206602747
76-77	22.22423561969672	27.344823544798746	27.322201775986425	23.108739059518104
78-79	22.364417990708837	27.228768645661294	26.843061626074594	23.56375173755528
80-81	22.31929028940368	27.614207326741635	26.726381738615252	23.34012064523943
82-83	22.224917926079694	27.701990205105837	26.84459111774464	23.22850075106983
84-85	22.34322152889462	27.670560384329594	26.780155696358182	23.2060623904176
86-87	22.469585809026213	27.955371061371597	26.79204245410942	22.78300067549277
88-89	22.61645209582208	27.61849360113469	27.058824214373946	22.706230088669283
90-91	22.382310643329266	27.64478039870412	27.247253669368675	22.725655288597935
92-93	22.292112232667918	27.20932212790422	27.258839724437113	23.239725914990757
94-95	22.07124289831138	27.197351161122313	27.82103241059735	22.910373529968957
96-97	21.709075902409268	27.455714997017015	27.998331322247694	22.836877778326027
98-99	21.87064339337062	27.597354819661096	28.322816084146535	22.20918570282175
100-101	20.65041485724182	31.288318716605374	26.10965843652233	21.951607989630475
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1609.0
1	1416.0
2	1833.5
3	2585.0
4	3475.0
5	5361.0
6	6965.5
7	8533.0
8	9804.5
9	9997.5
10	9356.5
11	8646.5
12	9581.0
13	12759.5
14	20290.0
15	30778.5
16	39237.5
17	43572.0
18	43917.5
19	41657.5
20	38320.0
21	34909.0
22	33087.5
23	35069.0
24	40637.5
25	49789.5
26	62111.0
27	75759.5
28	91010.0
29	107909.0
30	125523.5
31	143199.0
32	159913.5
33	175927.5
34	191929.0
35	206835.0
36	223123.5
37	239179.5
38	252385.5
39	264286.0
40	275725.5
41	286177.0
42	292967.0
43	297854.0
44	302900.5
45	306585.5
46	309378.0
47	311784.5
48	313748.5
49	310948.0
50	304430.0
51	295798.0
52	282497.5
53	267625.0
54	252351.0
55	235104.0
56	216255.5
57	194852.5
58	171085.5
59	146945.5
60	122225.0
61	99010.0
62	78184.5
63	59747.0
64	44271.5
65	33506.0
66	26053.0
67	19621.5
68	14569.5
69	10646.0
70	7408.0
71	5058.0
72	3394.5
73	2189.0
74	1436.0
75	936.5
76	595.5
77	369.5
78	237.5
79	154.5
80	100.5
81	72.0
82	58.5
83	43.0
84	32.0
85	24.0
86	17.5
87	16.0
88	13.5
89	10.0
90	6.5
91	4.5
92	3.5
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.6344897118566392E-5
2	0.016281146419274032
3	2.6344897118566392E-5
4	1.8441427982996477E-4
5	2.6344897118566392E-5
6	1.3172448559283196E-5
7	1.3172448559283196E-5
8	0.0
9	0.0
10-11	1.3831070987247357E-4
12-13	5.861739608881022E-4
14-15	2.9638009258387194E-4
16-17	0.0016992458641475327
18-19	0.0
20-21	0.0
22-23	2.305426757217682E-4
24-25	0.009216969976790891
26-27	0.020068015083502384
28-29	0.3542496540942809
30-31	0.3301094860158406
32-33	0.3242182249602855
34-35	0.18959063932540282
36-37	0.04730751235434919
38-39	0.03856872189874682
40-41	0.016785460479898602
42-43	0.015756139150901998
44-45	0.019248846726223526
46-47	0.013145098647206586
48-49	0.008038149405048194
50-51	0.004649351909796683
52-53	0.0019614190288125403
54-55	2.897179659198397E-4
56-57	5.095841453651057E-4
58-59	0.001779346312116909
60-61	0.0017215394318606868
62-63	5.455006781075863E-4
64-65	2.753405709259196E-4
66-67	0.0032804444650773984
68-69	0.005284554771079601
70-71	0.006672109572195853
72-73	0.010558709941807676
74-75	0.007629851829652217
76-77	0.005620382967336774
78-79	0.003427174952363049
80-81	0.004416179491230842
82-83	0.001519309018258496
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.002812490498342911
92-93	0.015094444660174823
94-95	0.001401334122634778
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	129.0
20-21	489.0
22-23	998.0
24-25	2618.0
26-27	3175.0
28-29	5484.0
30-31	15269.0
32-33	19947.0
34-35	28941.0
36-37	39176.0
38-39	34424.0
40-41	49499.0
42-43	57516.0
44-45	67418.0
46-47	66385.0
48-49	56231.0
50-51	54459.0
52-53	10815.0
54-55	11099.0
56-57	11429.0
58-59	12061.0
60-61	62207.0
62-63	64414.0
64-65	70315.0
66-67	73537.0
68-69	71470.0
70-71	69834.0
72-73	68226.0
74-75	70185.0
76-77	70589.0
78-79	75083.0
80-81	76474.0
82-83	75946.0
84-85	78324.0
86-87	83845.0
88-89	89741.0
90-91	98269.0
92-93	106378.0
94-95	126396.0
96-97	195153.0
98-99	785030.0
100-101	4632625.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.30928183356518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.56278032638974	76.40536468661669
2	7.345856784516679	12.533395334786803
3	1.7687218679679146	4.526651769589941
4	0.6349274377846781	2.166608149353461
5	0.28031564309536267	1.195676309958968
6	0.1432917512974598	0.7334469837516078
7	0.0827072750525456	0.4938988766000665
8	0.04685678511560915	0.3197854949793843
9	0.03000838065986113	0.23039940627728994
>10	0.10328709292202606	1.2938315956475905
>50	9.217387367760672E-4	0.049733970772538
>100	3.249164612525232E-4	0.05120742166558824
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3172448559283198E-5	0.0	0.0	0.0
3	0.0	1.3172448559283198E-5	0.0	0.0	0.0
4	0.0	2.6344897118566396E-5	0.0	3.951734567784959E-5	0.0
5	0.0	2.6344897118566396E-5	0.0	5.268979423713279E-5	0.0
6	0.0	2.6344897118566396E-5	0.0	5.268979423713279E-5	0.0
7	0.0	2.6344897118566396E-5	0.0	5.268979423713279E-5	0.0
8	0.0	2.6344897118566396E-5	0.0	6.586224279641599E-5	1.3172448559283198E-5
9	0.0	2.6344897118566396E-5	0.0	6.586224279641599E-5	1.3172448559283198E-5
10-11	0.0	2.6344897118566396E-5	0.0	7.903469135569919E-5	1.3172448559283198E-5
12-13	0.0	3.951734567784959E-5	0.0	7.903469135569919E-5	1.3172448559283198E-5
14-15	6.586224279641599E-6	9.220713991498238E-5	0.0	9.220713991498238E-5	1.3172448559283198E-5
16-17	2.6344897118566396E-5	1.0537958847426558E-4	0.0	1.1855203703354878E-4	1.3172448559283198E-5
18-19	3.951734567784959E-5	1.2513826131319039E-4	0.0	1.7782805555032318E-4	1.3172448559283198E-5
20-21	3.951734567784959E-5	1.4489693415211516E-4	0.0	2.5686274690602234E-4	1.3172448559283198E-5
22-23	3.951734567784959E-5	1.7124183127068156E-4	0.0	5.005530452527615E-4	1.9758672838924797E-5
24-25	3.951734567784959E-5	1.9758672838924798E-4	0.0	0.0012843137345301117	2.6344897118566396E-5
26-27	3.951734567784959E-5	2.1075917694853116E-4	0.0	0.0035829060081250296	2.6344897118566396E-5
28-29	3.951734567784959E-5	2.2393162550781435E-4	0.0	0.01977843151176372	2.6344897118566396E-5
30-31	3.951734567784959E-5	2.5027652262638077E-4	0.0	0.029051835297499093	2.6344897118566396E-5
32-33	3.951734567784959E-5	2.5027652262638077E-4	0.0	0.03365560606896857	2.6344897118566396E-5
34-35	5.268979423713279E-5	3.2931121398207993E-4	0.0	0.03931975894946034	2.6344897118566396E-5
36-37	6.586224279641599E-5	4.149321296174207E-4	0.0	0.04943619944298984	2.6344897118566396E-5
38-39	6.586224279641599E-5	4.544494752952703E-4	0.0	0.07068335896911364	2.6344897118566396E-5
40-41	6.586224279641599E-5	5.334841666509696E-4	0.0	0.26722287769789854	2.6344897118566396E-5
42-43	6.586224279641599E-5	5.532428394898943E-4	0.0	0.2877192076561432	2.6344897118566396E-5
44-45	7.24484670760576E-5	6.322775308455935E-4	0.0	0.3242529937353152	2.6344897118566396E-5
46-47	7.903469135569919E-5	7.178984464809343E-4	0.0	0.3842798418199687	2.6344897118566396E-5
48-49	7.903469135569919E-5	7.310708950402174E-4	0.0	0.4296128235367418	3.2931121398207994E-5
50-51	7.903469135569919E-5	8.298642592348414E-4	0.0	0.5220899986471895	3.951734567784959E-5
52-53	7.903469135569919E-5	8.298642592348414E-4	0.0	0.693463554403464	3.951734567784959E-5
54-55	7.903469135569919E-5	8.298642592348414E-4	0.0	1.3062063440356404	3.951734567784959E-5
56-57	7.903469135569919E-5	8.430367077941247E-4	0.0	1.9726466202197348	4.610356995749119E-5
58-59	7.903469135569919E-5	8.496229320737662E-4	0.0	2.6540046943972175	6.586224279641599E-5
60-61	9.220713991498238E-5	8.562091563534078E-4	0.0	3.168764225421166	6.586224279641599E-5
62-63	9.220713991498238E-5	8.69381604912691E-4	0.0	3.1794536674270244	6.586224279641599E-5
64-65	9.220713991498238E-5	0.001001106090505523	0.0	3.1867248590317487	6.586224279641599E-5
66-67	1.0537958847426558E-4	0.001001106090505523	0.0	3.1926590471077056	6.586224279641599E-5
68-69	1.0537958847426558E-4	0.0010076923147851647	0.0	3.1982309928482824	6.586224279641599E-5
70-71	1.0537958847426558E-4	0.0010406234361833725	0.0	3.203743662570343	6.586224279641599E-5
72-73	1.0537958847426558E-4	0.0010735545575815807	0.0	3.2085911236401587	6.586224279641599E-5
74-75	1.0537958847426558E-4	0.0011064856789797886	0.0	3.2123847888252324	6.586224279641599E-5
76-77	1.0537958847426558E-4	0.0011789341460558462	0.0	3.215941349936239	6.586224279641599E-5
78-79	1.0537958847426558E-4	0.001198692818894771	0.0	3.219155427384704	6.586224279641599E-5
80-81	1.0537958847426558E-4	0.0012184514917336958	0.0	3.2206505002961823	6.586224279641599E-5
82-83	1.0537958847426558E-4	0.0012250377160133374	0.0	3.221177398238554	6.586224279641599E-5
84-85	1.0537958847426558E-4	0.0012382101645726205	0.0	3.221519881901095	6.586224279641599E-5
86-87	1.0537958847426558E-4	0.0012513826131319039	0.0	3.2216055028167307	6.586224279641599E-5
88-89	1.0537958847426558E-4	0.0013304173044876029	0.0	3.2216252614895695	6.586224279641599E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9040	0.0	48.91552	1
GTATCAA	16780	0.0	37.587776	1
TCAACGC	20285	0.0	30.450632	4
ATCAACG	20455	0.0	30.134296	3
CAACGCA	20895	0.0	29.561668	5
TATCAAC	21185	0.0	29.324137	2
AACGCAG	21465	0.0	28.897995	6
TATCTCG	9160	0.0	27.93406	94-95
ACGCAGA	24870	0.0	24.941515	7
CGCAGAG	25400	0.0	24.438065	8
CTATCTC	14595	0.0	21.103506	94-95
GCAGAGT	29390	0.0	20.97357	9
GTACATG	21065	0.0	20.479992	1
TACATGG	21470	0.0	19.711887	2
GAGTACT	17155	0.0	19.311752	12-13
ACATGGG	21450	0.0	19.003466	3
AGAGTAC	26585	0.0	18.992853	10-11
CGCTATC	14455	0.0	18.458733	92-93
CAGAGTA	28930	0.0	18.079597	10-11
GTGGTAT	5325	0.0	17.90457	1
>>END_MODULE