FastQCFastQC Report
Fri 27 May 2016
SRR2139082_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139082_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9137892
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151450.038.5311161
GTATCAA247850.031.3928471
ATCAACG291700.026.1241743
TCAACGC292200.026.123694
CAACGCA295750.025.795465
AACGCAG305400.025.0366086
TATCAAC310200.024.8475782
ACGCAGA344400.022.0643317
CGCAGAG348750.021.813748
GTACATG258550.019.7468911
TATCTCG175350.019.64298894-95
GAGTACT219500.019.23667312-13
TACATGG253800.019.033662
TCGTATC8600.018.96932294-95
GCAGAGT400550.018.939159
AGAGTAC331600.018.902910-11
ACATGGG253700.018.4791853
GGACCGA11550.018.2135546
CAGAGTA378850.017.34994110-11
GTACTTT245300.017.21425814-15
Produced by FastQC (version 0.11.5)