##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139082_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9137892
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61658925275107	34.0	31.0	34.0	31.0	34.0
2	32.74816456574448	34.0	31.0	34.0	31.0	34.0
3	32.73653048208493	34.0	31.0	34.0	31.0	34.0
4	36.2407587001466	37.0	37.0	37.0	35.0	37.0
5	36.200406176829404	37.0	37.0	37.0	35.0	37.0
6	36.24231113696682	37.0	37.0	37.0	35.0	37.0
7	36.20195653439546	37.0	37.0	37.0	35.0	37.0
8	36.18128010267576	37.0	37.0	37.0	35.0	37.0
9	37.92256638620811	39.0	38.0	39.0	35.0	39.0
10-11	37.85394766101416	39.0	38.0	39.0	35.0	39.0
12-13	37.824650969829804	39.0	38.0	39.0	35.0	39.0
14-15	39.183872330730104	41.0	39.0	41.0	36.0	41.0
16-17	39.15362881285968	40.0	39.0	41.0	36.0	41.0
18-19	39.11709385490658	40.0	39.0	41.0	36.0	41.0
20-21	39.106718814169426	40.0	39.0	41.0	36.0	41.0
22-23	38.99547531932684	40.0	38.5	41.0	35.0	41.0
24-25	38.86008261329452	40.0	38.0	41.0	35.0	41.0
26-27	38.80998452259837	40.0	38.0	41.0	35.0	41.0
28-29	38.753587991939696	40.0	38.0	41.0	35.0	41.0
30-31	38.68634499895769	40.0	38.0	41.0	35.0	41.0
32-33	38.579167325927614	40.0	38.0	41.0	34.5	41.0
34-35	38.38675441666441	40.0	38.0	41.0	34.0	41.0
36-37	38.527635005688516	40.0	38.0	41.0	34.0	41.0
38-39	38.511512173895866	40.0	38.0	41.0	34.5	41.0
40-41	38.489485659575394	40.0	38.0	41.0	34.0	41.0
42-43	38.52466851114738	40.0	38.0	41.0	34.0	41.0
44-45	38.46606682621387	40.0	38.0	41.0	34.0	41.0
46-47	38.41047754899405	40.0	38.0	41.0	34.0	41.0
48-49	38.252355297965465	40.0	38.0	41.0	33.0	41.0
50-51	38.24091887879541	40.0	38.0	41.0	33.0	41.0
52-53	38.15771048984916	40.0	37.0	41.0	33.0	41.0
54-55	38.0249584130665	40.0	37.0	41.0	33.0	41.0
56-57	37.85950499085135	40.0	37.0	41.0	33.0	41.0
58-59	37.70887000295072	39.5	36.0	41.0	33.0	41.0
60-61	37.53628319760373	39.0	36.0	41.0	33.0	41.0
62-63	37.34113339254179	39.0	35.5	41.0	32.0	41.0
64-65	37.07948449213221	38.5	35.0	40.0	32.0	41.0
66-67	36.745285695577465	38.0	35.0	40.0	31.0	41.0
68-69	36.42285894708624	37.5	35.0	40.0	31.0	41.0
70-71	36.128181568597334	37.0	35.0	39.5	31.0	41.0
72-73	35.8118336172397	36.5	34.5	39.0	31.0	41.0
74-75	35.44046151046153	36.0	34.0	39.0	30.5	40.0
76-77	34.468927027389434	35.0	33.5	37.0	29.5	39.0
78-79	34.714724716330025	35.0	34.0	37.0	30.0	39.0
80-81	34.49401830600026	35.0	34.0	37.0	30.0	39.0
82-83	34.20652530259638	35.0	34.0	36.0	30.0	38.5
84-85	33.95818516469064	35.0	34.0	36.0	30.0	37.0
86-87	33.72331224589384	35.0	33.0	35.5	29.0	37.0
88-89	33.484988432538174	35.0	33.0	35.0	29.0	36.5
90-91	33.30831843250961	35.0	33.0	35.0	29.0	36.0
92-93	33.22513286407238	35.0	33.0	35.0	29.0	36.0
94-95	33.14343353750016	35.0	33.0	35.0	29.0	36.0
96-97	33.10320093350095	34.5	33.0	35.0	29.0	35.0
98-99	33.200498628247374	34.0	33.0	35.0	29.0	35.0
100-101	31.33084302152545	32.5	29.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	1.0
17	0.0
18	1.0
19	13.0
20	44.0
21	236.0
22	1271.0
23	4056.0
24	10607.0
25	21523.0
26	38687.0
27	60343.0
28	86745.0
29	116730.0
30	149796.0
31	189001.0
32	239313.0
33	312867.0
34	436604.0
35	628102.0
36	1042355.0
37	1977366.0
38	2564153.0
39	1183670.0
40	74407.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.51811101708261	21.61459356431369	15.50118074290884	24.36611467569486
2	13.51981926041996	21.612044080720743	41.86429448704221	23.00384217181709
3	16.804643434683122	26.812423129144353	31.5105640896789	24.872369346493628
4	11.788191180407013	18.997454771247867	39.07861799922564	30.135736049119476
5	12.627891928235957	38.22328368766929	36.15439273679233	12.994431647302424
6	29.214377803368414	38.73310592126783	18.767043730331213	13.285472545032546
7	25.688356317666734	33.208516056932616	23.297224709727878	17.805902915672775
8	24.01362371102657	37.20532043933108	21.30670837431653	17.474347475325818
9	25.00228717958146	17.00074809376167	21.586149190644843	36.410815536012024
10-11	23.63301185471774	27.166440447189018	29.273405946083127	19.927141752010122
12-13	24.527318209507758	24.682382317427866	29.334124987066133	21.45617448599824
14-15	21.642145360031677	26.212696515309176	26.96476459857519	25.18039352608395
16-17	21.139380751299175	29.484106425696215	27.642481416038443	21.73403140696616
18-19	21.252379651674588	28.48053467911418	29.413791495894237	20.853294173316996
20-21	21.89586168151292	27.51582313373424	29.425045155992755	21.16327002876008
22-23	21.589961410456965	27.67134832459872	29.26057094354894	21.478119321395376
24-25	21.532530923556624	27.804508225753104	29.323826354445593	21.33913449624468
26-27	21.510917557695418	27.935312254769013	29.325644627516173	21.2281255600194
28-29	21.380936665144333	27.933904761999013	29.31523396891564	21.36992460394102
30-31	21.478904996698056	27.59635497818394	29.47197875309236	21.452761272025647
32-33	21.25777988747183	27.93227021739427	29.258487579893217	21.551462315240684
34-35	21.535672047683175	27.879956918865744	29.270386493730705	21.31398453972038
36-37	21.378219564810685	27.968380236046002	29.343952019407165	21.309448179736147
38-39	21.386077415072187	27.893938503014322	29.283828232922403	21.43615584899109
40-41	21.49156867359633	27.719332549086406	29.26969566504515	21.51940311227212
42-43	21.64733980431612	27.773965415535674	29.043214139657064	21.535480640491144
44-45	21.49668440393636	28.0150029967986	28.855915948988308	21.632396650276732
46-47	21.722543203807238	27.824368851452082	28.489403470692153	21.963684474048527
48-49	21.68549092990625	28.035771697349443	28.42440258361846	21.85433478912585
50-51	21.421652205285373	28.13595890817404	28.552644064104456	21.88974482243613
52-53	21.5568757981827	28.083315327885096	28.387454415618468	21.97235445831374
54-55	21.550821247632435	27.912793618929122	28.34732004312962	22.189065090308826
56-57	21.817766630647593	27.80785471022364	28.26585456586382	22.108524093264954
58-59	21.38843888393298	27.925013137039866	28.725490451461866	21.961057527565288
60-61	21.649471969899224	27.84063307720162	28.643360127766577	21.86653482513258
62-63	21.225004860305773	28.385014534671594	28.60657360785197	21.783406997170662
64-65	20.891520086699263	28.517945023958212	28.506596349005576	22.083938540336952
66-67	21.048614585525172	28.821167500249423	28.083922705631164	22.04629520859424
68-69	21.17785620768902	28.904056588048228	27.800287889865384	22.117799314397367
70-71	21.14981151905437	28.487527054572	27.82500914908677	22.537652277286867
72-73	21.13926386064672	28.150070900735596	27.833603060126787	22.877062178490892
74-75	21.4373099662135	27.933655926802647	27.801261889313928	22.827772217669924
76-77	21.78395208371767	27.76420475317073	27.67027268661062	22.78157047650098
78-79	21.960883079633213	27.620581482462285	27.190503053298876	23.228032384605623
80-81	21.889656882658056	28.004602854550686	27.081153661212877	23.024586601578378
82-83	21.797860350938933	28.07532384045443	27.262470670976803	22.864345137629833
84-85	21.99521753818972	28.066486797048906	27.128512568824593	22.80978309593678
86-87	22.072548384440267	28.44456052651391	27.10960980148235	22.373281287563476
88-89	22.301593387126804	28.04476043599938	27.36710019258005	22.286545984293774
90-91	22.069828490462278	28.051985648661272	27.59739900373551	22.28078685714094
92-93	21.87809864392458	27.628949193632145	27.61802546877715	22.874926693666122
94-95	21.674304855178285	27.59628742238041	28.19851552628942	22.530892196151882
96-97	21.29244896965824	27.87677526678597	28.407275619999794	22.423500143555998
98-99	21.471312617851623	28.034664853133627	28.669077343815868	21.824945185198878
100-101	20.23543957776186	31.757149541726736	26.59839541541844	21.409015465092963
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1562.0
1	1498.5
2	2274.5
3	3333.5
4	4607.0
5	7129.5
6	9202.5
7	10989.0
8	12436.5
9	12713.0
10	11943.0
11	11172.5
12	12525.0
13	16784.5
14	26978.0
15	41263.5
16	52650.0
17	58451.5
18	58888.0
19	55677.0
20	51210.0
21	46759.5
22	44634.5
23	47554.0
24	55115.5
25	67154.0
26	83741.5
27	102252.5
28	122871.0
29	145673.5
30	167390.0
31	188363.0
32	209724.0
33	232428.5
34	252534.0
35	269386.0
36	289399.0
37	309472.5
38	324317.0
39	333936.5
40	342964.0
41	351107.0
42	355993.0
43	361959.0
44	368122.0
45	373370.5
46	378581.5
47	385542.5
48	394984.0
49	385716.5
50	365748.0
51	349569.5
52	327834.5
53	306495.0
54	284987.0
55	259476.5
56	233339.5
57	206775.5
58	180034.0
59	153034.5
60	124742.5
61	100011.5
62	78443.5
63	59323.5
64	43937.0
65	33070.0
66	25017.5
67	18685.5
68	14179.5
69	9913.0
70	6686.0
71	4668.0
72	3174.5
73	2149.5
74	1396.0
75	879.0
76	574.5
77	358.0
78	224.5
79	147.0
80	105.0
81	81.0
82	57.5
83	35.0
84	22.0
85	16.5
86	13.0
87	10.0
88	6.5
89	4.0
90	2.5
91	2.0
92	2.0
93	1.5
94	1.5
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0943442973499795E-5
2	0.018089511235195165
3	1.0943442973499795E-5
4	1.0943442973499795E-4
5	1.0943442973499795E-5
6	1.0943442973499795E-5
7	1.0943442973499795E-5
8	0.0
9	0.0
10-11	1.2037787270849776E-4
12-13	6.40191413949738E-4
14-15	2.462274669037454E-4
16-17	0.0019041590773889647
18-19	0.0
20-21	0.0
22-23	1.8059315328670237E-4
24-25	0.009212415138904664
26-27	0.019532508813585994
28-29	0.34731602575225007
30-31	0.3239634734803722
32-33	0.3181794792405166
34-35	0.1881501524132591
36-37	0.04771819247364186
38-39	0.03859871725007762
40-41	0.016386652900459958
42-43	0.01518071679036338
44-45	0.01860206663840518
46-47	0.012662220335273073
48-49	0.00774846847402787
50-51	0.004834745250432789
52-53	0.001965261935877151
54-55	2.1809976979864028E-4
56-57	5.552270134244441E-4
58-59	0.001728683789634812
60-61	0.0014567365593012183
62-63	3.543491189259306E-4
64-65	1.518012001767209E-4
66-67	0.0035160397491981804
68-69	0.0059190317534580745
70-71	0.0072834835019341205
72-73	0.01113616713363267
74-75	0.007892457261572049
76-77	0.005919191201714198
78-79	0.0038168069540643335
80-81	0.005036741899556573
82-83	0.0017676715542110455
84-85	0.0
86-87	0.0
88-89	7.028211945570716E-6
90-91	0.002884866341071892
92-93	0.01621942734230658
94-95	0.001600836703983949
96-97	7.593546032607597E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	221.0
20-21	845.0
22-23	1361.0
24-25	3588.0
26-27	4514.0
28-29	7083.0
30-31	19501.0
32-33	25364.0
34-35	36670.0
36-37	49123.0
38-39	43742.0
40-41	62317.0
42-43	73131.0
44-45	86203.0
46-47	83709.0
48-49	70547.0
50-51	68547.0
52-53	15102.0
54-55	16494.0
56-57	18893.0
58-59	21041.0
60-61	83713.0
62-63	88399.0
64-65	96057.0
66-67	105019.0
68-69	91911.0
70-71	86706.0
72-73	83631.0
74-75	86117.0
76-77	87477.0
78-79	92169.0
80-81	94527.0
82-83	93276.0
84-85	96826.0
86-87	102973.0
88-89	110066.0
90-91	119642.0
92-93	127729.0
94-95	152332.0
96-97	228818.0
98-99	922616.0
100-101	5479892.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.0242504428869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.16361657580651	73.19718182540414
2	8.034609969554838	13.341349406464737
3	2.11779366184464	5.274846941020438
4	0.7738836891399075	2.570044528832676
5	0.372683624266814	1.5470889278545361
6	0.18939249202211172	0.9434501813787757
7	0.10703213490757472	0.6220383941802337
8	0.06760649118895487	0.44903826048292916
9	0.043796257978274356	0.32725363417645803
>10	0.12756622749461935	1.5402261807345652
>50	0.0015588163552113273	0.08540244016461584
>100	3.953735963415548E-4	0.05784431757813213
>500	6.468584417107253E-5	0.04423496172776104
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.566065784099877E-5	0.0	0.0	0.0
3	0.0	6.566065784099877E-5	0.0	1.0943442973499797E-5	0.0
4	0.0	1.0943442973499796E-4	0.0	1.0943442973499797E-5	0.0
5	0.0	1.0943442973499796E-4	0.0	1.0943442973499797E-5	0.0
6	1.2037787270849776E-4	1.0943442973499796E-4	0.0	2.1886885946999594E-5	0.0
7	1.2037787270849776E-4	1.0943442973499796E-4	0.0	2.1886885946999594E-5	0.0
8	1.2037787270849776E-4	1.3132131568199755E-4	0.0	4.377377189399919E-5	0.0
9	1.2037787270849776E-4	1.7509508757599675E-4	0.0	5.471721486749898E-5	0.0
10-11	1.3679303716874745E-4	1.7509508757599675E-4	0.0	1.4773648014224726E-4	0.0
12-13	1.5867992311574704E-4	1.7509508757599675E-4	0.0	2.2434058095674583E-4	0.0
14-15	1.9698197352299634E-4	2.1886885946999592E-4	0.0	3.501901751519935E-4	0.0
16-17	3.8849222555924277E-4	2.2981230244349573E-4	0.0	6.40191413949738E-4	0.0
18-19	3.939639470459927E-4	2.407557454169955E-4	0.0	9.794381461282317E-4	0.0
20-21	3.939639470459927E-4	2.516991883904953E-4	0.0	0.00160868611710447	0.0
22-23	4.0490739001949243E-4	3.8849222555924277E-4	0.0	0.0029766164887919445	0.0
24-25	4.4320944042674175E-4	4.650963263737413E-4	0.0	0.005143418197544904	0.0
26-27	4.5962460488699146E-4	4.8698321232074093E-4	0.0	0.008929849466375834	0.0
28-29	4.5962460488699146E-4	4.979266552942406E-4	0.0	0.025793695088539022	0.0
30-31	4.924549338074908E-4	5.417004271882399E-4	0.0	0.0351448671093946	0.0
32-33	4.924549338074908E-4	5.471721486749898E-4	0.0	0.03960432012109576	2.1886885946999594E-5
34-35	5.635873131352395E-4	6.128328065159885E-4	0.0	0.04537698628961691	2.1886885946999594E-5
36-37	6.128328065159885E-4	6.949086288172372E-4	0.0	0.05462419560222423	2.1886885946999594E-5
38-39	6.128328065159885E-4	7.113237932774867E-4	0.0	0.07656032704260457	2.735860743374949E-5
40-41	6.237762494894884E-4	7.332106792244863E-4	0.0	0.2748336268364739	3.283032892049939E-5
42-43	6.237762494894884E-4	7.441541221979861E-4	0.0	0.29603107587614297	3.283032892049939E-5
44-45	6.292479709762383E-4	7.988713370654852E-4	0.0	0.33263142090101305	3.8302050407249284E-5
46-47	6.347196924629882E-4	8.426451089594843E-4	0.0	0.39251941257349066	4.377377189399919E-5
48-49	6.347196924629882E-4	8.426451089594843E-4	0.0	0.43939017882899034	5.471721486749898E-5
50-51	6.347196924629882E-4	9.520795386944822E-4	0.0	0.531960762941825	5.471721486749898E-5
52-53	6.566065784099878E-4	9.520795386944822E-4	0.0	0.7070832091252556	6.018893635424888E-5
54-55	6.620782998967376E-4	9.575512601812321E-4	0.0	1.33212889799967	7.113237932774866E-5
56-57	6.675500213834876E-4	9.739664246414819E-4	0.0	2.0126523710282416	7.660410081449857E-5
58-59	6.675500213834876E-4	9.849098676149816E-4	0.0	2.7140559332502505	7.660410081449857E-5
60-61	6.839651858437372E-4	9.849098676149816E-4	0.0	3.2405176160978924	7.660410081449857E-5
62-63	7.550975651714859E-4	9.849098676149816E-4	0.0	3.2512750205408425	7.660410081449857E-5
64-65	7.550975651714859E-4	0.0011381180692439789	0.0	3.2580380682984655	7.660410081449857E-5
66-67	7.605692866582358E-4	0.0011381180692439789	0.0	3.263624695936437	8.207582230124847E-5
68-69	7.824561726052354E-4	0.0011709483981644782	0.0	3.269047171929806	9.301926527474828E-5
70-71	8.098147800389849E-4	0.001181891841137978	0.0	3.2742015335703245	1.2037787270849776E-4
72-73	8.590602734197339E-4	0.001181891841137978	0.0	3.2782232488630854	1.4226475865549736E-4
74-75	8.645319949064839E-4	0.0012311373345187272	0.0	3.282234020712873	1.4226475865549736E-4
76-77	8.809471593667335E-4	0.0013351000427669752	0.0	3.2857523376288533	1.4773648014224723E-4
78-79	8.973623238269833E-4	0.0013569869287139748	0.0	3.28883838854738	1.5320820162899714E-4
80-81	8.973623238269833E-4	0.0013569869287139748	0.0	3.2901351865397404	1.5867992311574704E-4
82-83	9.028340453137332E-4	0.0013569869287139748	0.0	3.290644056638008	1.7509508757599675E-4
84-85	9.137774882872331E-4	0.0013679303716874745	0.0	3.2909833033701865	1.7509508757599675E-4
86-87	9.301926527474826E-4	0.0013679303716874745	0.0	3.291207643951143	1.7509508757599675E-4
88-89	9.520795386944822E-4	0.0014117041435814737	0.0	3.291306134937905	1.7509508757599675E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15145	0.0	38.531116	1
GTATCAA	24785	0.0	31.392847	1
ATCAACG	29170	0.0	26.124174	3
TCAACGC	29220	0.0	26.12369	4
CAACGCA	29575	0.0	25.79546	5
AACGCAG	30540	0.0	25.036608	6
TATCAAC	31020	0.0	24.847578	2
ACGCAGA	34440	0.0	22.064331	7
CGCAGAG	34875	0.0	21.81374	8
GTACATG	25855	0.0	19.746891	1
TATCTCG	17535	0.0	19.642988	94-95
GAGTACT	21950	0.0	19.236673	12-13
TACATGG	25380	0.0	19.03366	2
TCGTATC	860	0.0	18.969322	94-95
GCAGAGT	40055	0.0	18.93915	9
AGAGTAC	33160	0.0	18.9029	10-11
ACATGGG	25370	0.0	18.479185	3
GGACCGA	1155	0.0	18.213554	6
CAGAGTA	37885	0.0	17.349941	10-11
GTACTTT	24530	0.0	17.214258	14-15
>>END_MODULE