FastQCFastQC Report
Fri 27 May 2016
SRR2139081_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139081_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8056079
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA194850.036.137921
GTATCAA316700.031.1118741
ATCAACG356050.027.0960243
TCAACGC356500.026.9572124
CAACGCA358350.026.7256165
AACGCAG366150.026.2021666
TATCAAC394200.024.7867012
ATCTCGT101900.022.90059994
ACGCAGA418600.022.7815977
CGCAGAG423050.022.5716698
GTACATG312400.021.2635841
TACATGG308200.021.2465932
ACATGGG314750.020.2819273
GCAGAGT469700.020.234629
GAGTACT252350.020.09349612-13
CAGAGTA429700.018.5785410-11
CATGGGG226850.018.5002234
AGAGTAC392650.018.37904710-11
GTACTTT280300.017.9475914-15
AGTACTT264300.017.52856612-13
Produced by FastQC (version 0.11.5)