FastQCFastQC Report
Fri 27 May 2016
SRR2139080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8614905
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112300.048.41551
GTATCAA202150.036.2898141
TCAACGC249300.028.8076654
ATCAACG252000.028.4819533
CAACGCA256200.028.0816655
TATCAAC262050.027.5721682
AACGCAG263500.027.3524766
ACGCAGA303450.023.7233927
CGCAGAG311850.023.0570748
GTACATG248550.020.5732291
TACATGG251450.019.9465082
GCAGAGT363950.019.6160539
ACATGGG254600.018.9445483
AGAGTAC324400.018.64881110-11
GAGTACT211700.018.30020512-13
GTGGTAT57800.018.0472681
CAGAGTA349200.017.84862710-11
CATGGGG188750.017.4569854
AGTACTT231850.017.28815512-13
TGGTATC59300.016.8009722
Produced by FastQC (version 0.11.5)