##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139080_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8614905 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.55523757952061 34.0 31.0 34.0 31.0 34.0 2 32.694748346035155 34.0 31.0 34.0 31.0 34.0 3 32.684190713652676 34.0 31.0 34.0 31.0 34.0 4 36.21142868087344 37.0 35.0 37.0 35.0 37.0 5 36.163818057192735 37.0 35.0 37.0 35.0 37.0 6 36.20582385992649 37.0 37.0 37.0 35.0 37.0 7 36.16377615307424 37.0 37.0 37.0 35.0 37.0 8 36.14072261969227 37.0 36.0 37.0 35.0 37.0 9 37.87067030919087 39.0 38.0 39.0 35.0 39.0 10-11 37.798316812547554 39.0 38.0 39.0 35.0 39.0 12-13 37.77091900607145 39.0 38.0 39.0 35.0 39.0 14-15 39.11575409131035 40.0 38.5 41.0 36.0 41.0 16-17 39.084480211911796 40.0 38.0 41.0 36.0 41.0 18-19 39.043477496269546 40.0 38.5 41.0 36.0 41.0 20-21 39.02370431867985 40.0 38.5 41.0 35.0 41.0 22-23 38.90721665806143 40.0 38.0 41.0 35.0 41.0 24-25 38.77063388206258 40.0 38.0 41.0 35.0 41.0 26-27 38.72000101983657 40.0 38.0 41.0 34.5 41.0 28-29 38.66376406556742 40.0 38.0 41.0 35.0 41.0 30-31 38.59786311412692 40.0 38.0 41.0 34.5 41.0 32-33 38.48740246800177 40.0 38.0 41.0 34.0 41.0 34-35 38.29661696968326 40.0 38.0 41.0 34.0 41.0 36-37 38.43602480727259 40.0 38.0 41.0 34.0 41.0 38-39 38.425569792506195 40.0 38.0 41.0 34.0 41.0 40-41 38.387460434287405 40.0 38.0 41.0 34.0 41.0 42-43 38.415239327973936 40.0 38.0 41.0 34.0 41.0 44-45 38.34520425251296 40.0 38.0 41.0 34.0 41.0 46-47 38.285110168199985 40.0 38.0 41.0 33.0 41.0 48-49 38.12716908403952 40.0 38.0 41.0 33.0 41.0 50-51 38.12637906605757 40.0 37.0 41.0 33.0 41.0 52-53 38.02628970921954 40.0 37.0 41.0 33.0 41.0 54-55 37.87219953604267 40.0 37.0 41.0 33.0 41.0 56-57 37.70248078190393 40.0 36.5 41.0 33.0 41.0 58-59 37.53173196087191 39.0 36.0 41.0 32.5 41.0 60-61 37.346808851868914 39.0 36.0 41.0 32.0 41.0 62-63 37.126929919968454 39.0 35.0 40.0 32.0 41.0 64-65 36.824532550315666 38.0 35.0 40.0 31.0 41.0 66-67 36.45841471831842 38.0 35.0 40.0 31.0 41.0 68-69 36.11808558164747 37.0 35.0 40.0 31.0 41.0 70-71 35.80905588036929 36.5 34.0 39.0 30.5 41.0 72-73 35.47948571527155 36.0 34.0 39.0 30.0 40.5 74-75 35.11154344806201 35.5 34.0 38.5 30.0 40.0 76-77 34.15681599055319 35.0 32.5 37.0 29.0 39.0 78-79 34.44181500859903 35.0 34.0 37.0 29.5 39.0 80-81 34.2502517696993 35.0 34.0 36.5 29.0 39.0 82-83 33.98540616391136 35.0 33.5 36.0 29.0 38.0 84-85 33.74836047334926 35.0 33.0 36.0 29.0 37.0 86-87 33.517977400663284 35.0 33.0 35.0 29.0 37.0 88-89 33.28941999332346 35.0 33.0 35.0 29.0 36.0 90-91 33.111063711567716 35.0 33.0 35.0 29.0 36.0 92-93 33.03178968581959 35.0 33.0 35.0 29.0 36.0 94-95 32.959261653848166 34.0 32.5 35.0 29.0 35.5 96-97 32.940996264536054 34.0 32.0 35.0 29.0 35.0 98-99 33.06832143774864 34.0 32.5 35.0 29.0 35.0 100-101 31.210120280645093 32.5 29.0 34.5 24.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 6.0 20 37.0 21 254.0 22 1142.0 23 4076.0 24 10280.0 25 21461.0 26 38041.0 27 60918.0 28 87071.0 29 116205.0 30 150280.0 31 189970.0 32 240171.0 33 314662.0 34 434757.0 35 630358.0 36 1051580.0 37 1923040.0 38 2248513.0 39 1033798.0 40 58284.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.80377541061398 21.27349300700275 15.582239802091816 24.340491780291458 2 13.731909868374615 21.72399349117482 41.15456184312691 23.389534797323655 3 16.989858505678065 26.497509432490485 31.211467939747212 25.30116412208424 4 12.127363204869708 18.713367204920782 38.80415188121961 30.355117708989898 5 13.080343089139474 37.905692274690466 35.653479133371654 13.360485502798406 6 29.85502798406111 38.312939993295345 18.606034379489316 13.225997643154235 7 26.218376896596872 32.97899779266258 23.19861022247027 17.60401508827028 8 24.375184636394714 36.892490398907476 21.052269293741485 17.680055670956325 9 25.4210580383649 16.890981386329855 21.3808161552565 36.30714442004874 10-11 23.96246431979769 26.987085683501828 29.008096614973844 20.04235338172664 12-13 24.804990642313506 24.549669680573402 29.172545570476444 21.47279410663665 14-15 21.974629942610925 25.968086612930186 26.843676290325575 25.213607154133317 16-17 21.459530346617417 29.078096510551806 27.523461919850906 21.938911222979872 18-19 21.49755568981898 28.19736839814252 29.371728417202515 20.933347494835985 20-21 22.27498334951224 27.33009755226947 29.258007832621608 21.136911265596684 22-23 22.018044867679563 27.20484725666606 29.150513439848467 21.626594435805913 24-25 21.806160392420853 27.411379633454246 29.1387824952763 21.6436774788486 26-27 21.77435975601964 27.523224303050174 29.093288495323538 21.60912744560665 28-29 21.70017768186562 27.61524512039559 29.082167105380847 21.602410092357953 30-31 21.94399930255593 27.44640236850886 28.989205605835096 21.620392723100114 32-33 21.734466028244793 27.584065269720977 28.959035866456823 21.722432835577415 34-35 21.897652487746452 27.64743916298808 28.88883670412121 21.566071645144255 36-37 21.88263571983721 27.55464483120413 28.883417157188696 21.679302291769964 38-39 21.810526939746342 27.68318659693916 28.87066476330406 21.635621700010443 40-41 22.00441089176092 27.52632830825048 28.754286840997956 21.714973958990644 42-43 21.90013571364242 27.675195524164792 28.540936614301636 21.883732147891152 44-45 21.97954672039173 27.72857503430944 28.346166012744323 21.945712232554506 46-47 22.069276601621997 27.75157027316886 28.107343161924014 22.071809963285137 48-49 22.025724539915387 27.89513863235581 28.002641787072452 22.07649504065635 50-51 22.030072340921585 27.95865160027385 27.92602880634846 22.085247252456114 52-53 22.190971052532166 27.85419585146342 27.779747106548875 22.17508598945554 54-55 22.096270463012115 27.811339922029614 27.8448583951418 22.247531219816473 56-57 22.09821038128423 27.88673142029992 27.854599658528734 22.160458539887117 58-59 22.0963870988437 27.937143966066497 27.814342631060455 22.152126304029345 60-61 22.13009100895086 27.871223739510693 27.85935233428434 22.139332917254116 62-63 22.111781142431028 27.95999028269315 27.798199853954365 22.13002872092146 64-65 22.101227553309048 27.941677375404485 27.738613727320416 22.218481343966058 66-67 22.13465157672563 27.987324820690684 27.619927550520273 22.25809605206341 68-69 22.099345494166606 27.956302337074955 27.648080931778512 22.296271236979926 70-71 22.081678716734153 27.78021619180321 27.73854503490467 22.39956005655797 72-73 22.131093257043432 27.791685931390266 27.645180530585723 22.432040280980583 74-75 22.0513147106656 27.845629774749348 27.631290070277725 22.471765444307323 76-77 22.207320508320045 27.808546402867414 27.580501939720282 22.403631149092266 78-79 22.179239674671784 27.783243505340288 27.543540336185497 22.493976483802427 80-81 22.163700911725655 27.813648563926698 27.54466240340065 22.477988120947 82-83 22.174837551211606 27.787261475697218 27.630592042187047 22.407308930904136 84-85 22.276667668622043 27.776279900148886 27.59950913971172 22.34754329151735 86-87 22.19278327598131 27.759391761373926 27.658535790854422 22.389289171790345 88-89 22.16254880869392 27.719828833073457 27.684116857252544 22.43350550098008 90-91 22.209819793667528 27.748147528907293 27.684260620239883 22.3577720571853 92-93 22.23410960799582 27.659840959428383 27.77242624172555 22.333623190850247 94-95 22.143097123636824 27.62451086270495 27.84141910698735 22.390972906670882 96-97 22.092554065230267 27.695575724845494 27.869184607327746 22.342685602596486 98-99 22.171100471028097 28.23693981227343 27.96154982196029 21.630409894738182 100-101 20.793018348383296 31.755116557599617 25.745564718246655 21.706300375770436 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2063.0 1 1806.0 2 2274.5 3 3166.5 4 4092.0 5 6032.5 6 7838.5 7 9982.5 8 11882.5 9 12221.5 10 11242.0 11 10308.0 12 11484.0 13 15498.5 14 25175.5 15 38409.5 16 48719.5 17 54024.0 18 54351.0 19 51178.0 20 46866.0 21 42678.0 22 40728.5 23 43505.5 24 50640.0 25 61863.5 26 76975.5 27 93569.5 28 111683.0 29 131279.5 30 150013.0 31 170098.0 32 190803.0 33 211351.0 34 230433.5 35 246572.0 36 265160.0 37 283424.0 38 298273.5 39 309191.0 40 320086.5 41 332212.5 42 339365.0 43 345235.0 44 351207.5 45 355461.5 46 357076.5 47 354724.5 48 350600.5 49 344877.5 50 337326.0 51 327101.0 52 311995.5 53 295537.5 54 279626.0 55 260628.0 56 239598.0 57 216965.5 58 192351.0 59 165800.0 60 138001.0 61 112901.0 62 90082.0 63 69182.0 64 51669.0 65 38411.5 66 29065.5 67 22391.5 68 17166.5 69 12275.5 70 8484.5 71 6049.5 72 4212.5 73 2748.0 74 1756.5 75 1128.0 76 741.0 77 479.0 78 311.5 79 204.5 80 137.5 81 93.0 82 65.5 83 47.5 84 33.0 85 20.0 86 16.0 87 15.5 88 11.0 89 6.0 90 2.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.1607789058614112E-5 2 0.01589106322124272 3 1.1607789058614112E-5 4 1.7411683587921167E-4 5 1.1607789058614112E-5 6 1.1607789058614112E-5 7 1.1607789058614112E-5 8 0.0 9 0.0 10-11 9.866620699821995E-5 12-13 7.196829216340749E-4 14-15 2.901947264653528E-4 16-17 0.0017121488861455813 18-19 0.0 20-21 0.0 22-23 1.9735591828025446E-4 24-25 0.00938206666724724 26-27 0.019119028613961028 28-29 0.3471692026577996 30-31 0.32372228982916634 32-33 0.3175929288625061 34-35 0.18626435685459086 36-37 0.04424307655719136 38-39 0.035694491274119236 40-41 0.014441550526098204 42-43 0.013130696471035633 44-45 0.01641396774383983 46-47 0.01114311356495056 48-49 0.006441169200049808 50-51 0.0038917775973045104 52-53 0.001423641045644367 54-55 1.1319546586757377E-4 56-57 2.3449159718374324E-4 58-59 0.0011118323821948095 60-61 0.0010182752042125142 62-63 2.600289425214473E-4 64-65 1.1152644872692887E-4 66-67 0.0036186976916751596 68-69 0.005645841392442636 70-71 0.007005624738262076 72-73 0.011088956760388303 74-75 0.008117378678550214 76-77 0.005991243975199466 78-79 0.003754723102252636 80-81 0.00475248329777679 82-83 0.0016884852048476774 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.002730266329044182 92-93 0.015287022036577986 94-95 0.00129096153205577 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 171.0 20-21 618.0 22-23 1178.0 24-25 2885.0 26-27 3769.0 28-29 6213.0 30-31 16548.0 32-33 22125.0 34-35 32210.0 36-37 44165.0 38-39 51334.0 40-41 56102.0 42-43 66512.0 44-45 79572.0 46-47 78433.0 48-49 69005.0 50-51 62240.0 52-53 55812.0 54-55 60251.0 56-57 64285.0 58-59 66634.0 60-61 66898.0 62-63 68325.0 64-65 75628.0 66-67 78719.0 68-69 78645.0 70-71 78396.0 72-73 77380.0 74-75 79320.0 76-77 79395.0 78-79 84662.0 80-81 86348.0 82-83 86044.0 84-85 88796.0 86-87 95665.0 88-89 103459.0 90-91 113297.0 92-93 123952.0 94-95 148458.0 96-97 228009.0 98-99 835528.0 100-101 5097919.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.44227700011405 #Duplication Level Percentage of deduplicated Percentage of total 1 90.16963751130177 77.04299145240519 2 6.819346065790534 11.653209110258253 3 1.6552273319181094 4.242791765757204 4 0.6019897262879936 2.057414917788863 5 0.2966836572193296 1.2674663610770422 6 0.15251380362980982 0.7818675993647526 7 0.08944076615033165 0.5349415901563342 8 0.05587405236196689 0.3819205007224041 9 0.033422187134239854 0.2570100993966032 >10 0.12351214344687939 1.6144905756256642 >50 0.002092522370503376 0.11743972008352367 >100 2.6023238862227135E-4 0.04845630736413116 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 2.3215578117228223E-5 0.0 0.0 0.0 3 0.0 2.3215578117228223E-5 0.0 0.0 0.0 4 0.0 4.6431156234456446E-5 0.0 0.0 0.0 5 0.0 5.8038945293070554E-5 0.0 0.0 0.0 6 6.964673435168466E-5 5.8038945293070554E-5 0.0 0.0 0.0 7 6.964673435168466E-5 6.964673435168466E-5 0.0 0.0 0.0 8 6.964673435168466E-5 8.125452341029878E-5 0.0 0.0 0.0 9 6.964673435168466E-5 1.5090125776198344E-4 0.0 0.0 1.1607789058614112E-5 10-11 6.964673435168466E-5 1.5090125776198344E-4 0.0 2.9019472646535277E-5 1.1607789058614112E-5 12-13 6.964673435168466E-5 1.5090125776198344E-4 0.0 6.384283982237762E-5 1.1607789058614112E-5 14-15 6.964673435168466E-5 1.8572462493782578E-4 0.0 9.866620699821995E-5 1.1607789058614112E-5 16-17 1.276856796447552E-4 1.973324139964399E-4 0.0 1.9733241399643987E-4 1.1607789058614112E-5 18-19 1.276856796447552E-4 2.0894020305505401E-4 0.0 3.366258826998092E-4 1.1607789058614112E-5 20-21 1.276856796447552E-4 2.205479921136681E-4 0.0 5.745855584013985E-4 1.1607789058614112E-5 22-23 1.276856796447552E-4 2.379596757015893E-4 0.0 0.001358111319857851 1.1607789058614112E-5 24-25 1.276856796447552E-4 2.6697914834812456E-4 0.0 0.0026349681163054034 1.1607789058614112E-5 26-27 1.276856796447552E-4 2.6697914834812456E-4 0.0 0.0052931518107280345 1.1607789058614112E-5 28-29 1.276856796447552E-4 2.727830428774316E-4 0.0 0.020824373571153715 1.1607789058614112E-5 30-31 1.3348957417406228E-4 3.3662588269980925E-4 0.0 0.02881633633800953 1.1607789058614112E-5 32-33 1.4509736323267637E-4 3.3662588269980925E-4 0.0 0.032664318410940106 1.1607789058614112E-5 34-35 1.5090125776198344E-4 3.772531444049586E-4 0.0 0.037533785921028726 1.1607789058614112E-5 36-37 1.5090125776198344E-4 4.294881951687221E-4 0.0 0.045427082480886324 1.1607789058614112E-5 38-39 1.5090125776198344E-4 4.4689987875664325E-4 0.0 0.06015156290173833 1.1607789058614112E-5 40-41 1.683129413499046E-4 4.759193514031786E-4 0.0 0.08922907449356668 1.1607789058614112E-5 42-43 1.7411683587921167E-4 4.933310349910997E-4 0.0 0.10343700830131035 1.1607789058614112E-5 44-45 1.7992073040851874E-4 5.687816638720914E-4 0.0 0.12384350146635395 1.1607789058614112E-5 46-47 1.8572462493782578E-4 6.26820609165162E-4 0.0 0.1495373425475963 1.7411683587921166E-5 48-49 1.8572462493782578E-4 6.616439763410043E-4 0.0 0.17143543660667182 2.3215578117228223E-5 50-51 2.1474409758436106E-4 7.080751325754608E-4 0.0 0.19668237780915748 2.3215578117228223E-5 52-53 2.205479921136681E-4 7.080751325754608E-4 0.0 0.22575988940098585 2.3215578117228223E-5 54-55 2.205479921136681E-4 7.19682921634075E-4 0.0 0.305905868956187 2.3215578117228223E-5 56-57 2.205479921136681E-4 7.661140778685313E-4 0.0 0.37617942391703685 2.3215578117228223E-5 58-59 2.205479921136681E-4 7.661140778685313E-4 0.0 0.4305503078675853 2.3215578117228223E-5 60-61 2.3215578117228222E-4 7.719179723978384E-4 0.0 0.45863535349490214 2.3215578117228223E-5 62-63 2.4376357023089633E-4 7.777218669271454E-4 0.0 0.4752170801651324 2.3215578117228223E-5 64-65 2.4376357023089633E-4 8.821919684546724E-4 0.0 0.4875445521453806 2.3215578117228223E-5 66-67 2.553713592895104E-4 8.879958629839796E-4 0.0 0.49499094882648154 2.3215578117228223E-5 68-69 2.553713592895104E-4 9.170153356305148E-4 0.0 0.502559227292698 2.3215578117228223E-5 70-71 2.6697914834812456E-4 9.170153356305148E-4 0.0 0.5093613916810458 2.3215578117228223E-5 72-73 2.7858693740673865E-4 9.228192301598218E-4 0.0 0.5139174488865519 3.482336717584233E-5 74-75 2.843908319360457E-4 9.634464918649712E-4 0.0 0.5179163322172444 3.482336717584233E-5 76-77 3.07606410053274E-4 9.924659645115064E-4 0.0 0.5214451000910632 3.482336717584233E-5 78-79 3.13410304582581E-4 9.982698590408135E-4 0.0 0.5245559875587718 3.482336717584233E-5 80-81 3.13410304582581E-4 0.0010040737535701207 0.0 0.5260766079254502 3.482336717584233E-5 82-83 3.13410304582581E-4 0.0010098776480994276 0.0 0.5265641350659119 4.6431156234456446E-5 84-85 3.13410304582581E-4 0.0010098776480994276 0.0 0.5269471921048462 4.6431156234456446E-5 86-87 3.1921419911188807E-4 0.0010098776480994276 0.0 0.5270864855735495 4.6431156234456446E-5 88-89 3.3662588269980925E-4 0.0010911321715097264 0.0 0.5271213089407254 4.6431156234456446E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11230 0.0 48.4155 1 GTATCAA 20215 0.0 36.289814 1 TCAACGC 24930 0.0 28.807665 4 ATCAACG 25200 0.0 28.481953 3 CAACGCA 25620 0.0 28.081665 5 TATCAAC 26205 0.0 27.572168 2 AACGCAG 26350 0.0 27.352476 6 ACGCAGA 30345 0.0 23.723392 7 CGCAGAG 31185 0.0 23.057074 8 GTACATG 24855 0.0 20.573229 1 TACATGG 25145 0.0 19.946508 2 GCAGAGT 36395 0.0 19.616053 9 ACATGGG 25460 0.0 18.944548 3 AGAGTAC 32440 0.0 18.648811 10-11 GAGTACT 21170 0.0 18.300205 12-13 GTGGTAT 5780 0.0 18.047268 1 CAGAGTA 34920 0.0 17.848627 10-11 CATGGGG 18875 0.0 17.456985 4 AGTACTT 23185 0.0 17.288155 12-13 TGGTATC 5930 0.0 16.800972 2 >>END_MODULE