FastQCFastQC Report
Fri 27 May 2016
SRR2139079_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139079_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9969733
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148350.054.7490351
GTATCAA284750.039.662011
TCAACGC371550.030.0372924
TATCAAC376250.029.7690242
ATCAACG375500.029.6647993
CAACGCA386400.028.9267375
AACGCAG393300.028.387676
ACGCAGA453150.024.6570427
CGCAGAG467350.023.8806828
GTGGTAT67450.022.475871
TGGTATC65250.021.6112142
GCAGAGT519300.021.3696129
GTACATG375150.021.2775611
TACATGG379750.020.6295322
GAGTACT289550.020.05553812-13
AGAGTAC476000.019.50284610-11
ACATGGG392400.019.4318983
CAGAGTA507250.018.79382710-11
GTACTTT319200.018.16635314-15
CATGGGG279600.018.034634
Produced by FastQC (version 0.11.5)