FastQCFastQC Report
Fri 27 May 2016
SRR2139078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11501972
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156950.048.0626371
GTATCAA282450.035.5894051
TCAACGC338800.029.1363684
ATCAACG339300.028.9804733
CAACGCA349800.028.2323065
TATCAAC360400.027.6791322
AACGCAG358600.027.5752336
ACGCAGA412200.023.9791857
CGCAGAG414200.023.8325588
GCAGAGT476900.020.5297059
GTACATG317550.020.0664181
GAGTACT286600.019.53294412-13
TACATGG318700.019.1520162
AGAGTAC426750.018.91119810-11
ACATGGG321300.018.4896583
CAGAGTA462700.018.44972410-11
GTACTTT325950.017.11015514-15
AGTACTT316150.016.98655112-13
GTGGTAT82250.016.416241
CATGGGG229450.016.2399754
Produced by FastQC (version 0.11.5)