##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139078_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11501972
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57612546787629	34.0	31.0	34.0	31.0	34.0
2	32.71151825095731	34.0	31.0	34.0	31.0	34.0
3	32.69920384087181	34.0	31.0	34.0	31.0	34.0
4	36.220959501553295	37.0	35.0	37.0	35.0	37.0
5	36.17697956489548	37.0	35.0	37.0	35.0	37.0
6	36.21765745908615	37.0	37.0	37.0	35.0	37.0
7	36.17832846402339	37.0	37.0	37.0	35.0	37.0
8	36.154418650993065	37.0	37.0	37.0	35.0	37.0
9	37.886604053635324	39.0	38.0	39.0	35.0	39.0
10-11	37.81587474739115	39.0	38.0	39.0	35.0	39.0
12-13	37.79103000772389	39.0	38.0	39.0	35.0	39.0
14-15	39.1426661010825	40.0	39.0	41.0	36.0	41.0
16-17	39.111186455679075	40.0	38.0	41.0	36.0	41.0
18-19	39.07297644264827	40.0	38.5	41.0	36.0	41.0
20-21	39.06001530271227	40.0	39.0	41.0	36.0	41.0
22-23	38.94197381274028	40.0	38.0	41.0	35.0	41.0
24-25	38.80990429974858	40.0	38.0	41.0	35.0	41.0
26-27	38.76169803904841	40.0	38.0	41.0	34.5	41.0
28-29	38.7022557370928	40.0	38.0	41.0	35.0	41.0
30-31	38.6356381282898	40.0	38.0	41.0	35.0	41.0
32-33	38.52465599566631	40.0	38.0	41.0	34.0	41.0
34-35	38.331741732200214	40.0	38.0	41.0	34.0	41.0
36-37	38.46961767999456	40.0	38.0	41.0	34.0	41.0
38-39	38.45426830337607	40.0	38.0	41.0	34.0	41.0
40-41	38.42669653531037	40.0	38.0	41.0	34.0	41.0
42-43	38.46202161011986	40.0	38.0	41.0	34.0	41.0
44-45	38.404277103913344	40.0	38.0	41.0	34.0	41.0
46-47	38.34986050455292	40.0	38.0	41.0	34.0	41.0
48-49	38.19839385682455	40.0	38.0	41.0	33.0	41.0
50-51	38.19500901595795	40.0	38.0	41.0	33.0	41.0
52-53	38.1051367456879	40.0	37.0	41.0	33.0	41.0
54-55	37.96168562830244	40.0	37.0	41.0	33.0	41.0
56-57	37.79469022359453	40.0	37.0	41.0	33.0	41.0
58-59	37.63666374594558	39.5	36.0	41.0	33.0	41.0
60-61	37.45885755526488	39.0	36.0	41.0	32.0	41.0
62-63	37.24998526634375	39.0	35.5	40.5	32.0	41.0
64-65	36.96297352606522	38.5	35.0	40.0	31.5	41.0
66-67	36.61198796241912	38.0	35.0	40.0	31.0	41.0
68-69	36.28078286495341	37.0	35.0	40.0	31.0	41.0
70-71	35.976984657749725	37.0	35.0	39.5	31.0	41.0
72-73	35.64896218181923	36.0	34.0	39.0	30.5	41.0
74-75	35.27876958247708	36.0	34.0	39.0	30.0	40.0
76-77	34.31481782055067	35.0	33.0	37.0	29.0	39.0
78-79	34.59465522223797	35.0	34.0	37.0	30.0	39.0
80-81	34.39474264323104	35.0	34.0	37.0	30.0	39.0
82-83	34.11841750543272	35.0	34.0	36.0	29.0	38.5
84-85	33.867835145004946	35.0	33.0	36.0	29.0	37.0
86-87	33.62795001352552	35.0	33.0	35.5	29.0	37.0
88-89	33.39213630605864	35.0	33.0	35.0	29.0	36.5
90-91	33.20809745665515	35.0	33.0	35.0	29.0	36.0
92-93	33.11671040438445	35.0	33.0	35.0	29.0	36.0
94-95	33.038634860933996	34.5	33.0	35.0	29.0	36.0
96-97	33.0162585489458	34.0	33.0	35.0	29.0	35.0
98-99	33.136754347582865	34.0	33.0	35.0	29.0	35.0
100-101	31.28277812177796	32.5	29.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	13.0
20	46.0
21	308.0
22	1508.0
23	5191.0
24	13389.0
25	27629.0
26	49670.0
27	77999.0
28	112802.0
29	151139.0
30	195456.0
31	247130.0
32	311881.0
33	406860.0
34	560851.0
35	810155.0
36	1345264.0
37	2512205.0
38	3111149.0
39	1476803.0
40	84522.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.26079358579443	21.584667669973058	15.831061983295037	24.323476760937474
2	13.465410158585234	21.743702785839282	41.638538711535745	23.152348344039737
3	16.75781626973466	26.72210064884537	31.539597129882967	24.980485951537
4	11.776169916128186	19.085552136910266	39.239896306341606	29.89838164061994
5	12.730793072838829	38.209506719283745	35.98403577821886	13.07566442965857
6	29.234861506333264	38.88325217332335	18.83196530681266	13.049921013530726
7	25.818255481452308	33.21973540184855	23.324543534716227	17.637465581982912
8	24.133652907518815	37.196282515728605	21.210954086829634	17.459110489922946
9	25.173144222573313	17.046807277917214	21.586133230023513	36.19391526948596
10-11	23.610455787733475	27.257487966176186	29.286764728257065	19.84529151783327
12-13	24.505256804711813	24.710901062750363	29.405855197073283	21.37798693546454
14-15	21.64679740464584	26.25502991365715	27.09433202424948	25.003840657447522
16-17	21.14022627022421	29.435034764406222	27.751273422627637	21.673465542741933
18-19	21.137815373651378	28.55330931745049	29.55178162282875	20.757093686069382
20-21	21.91670712469326	27.55055543422012	29.557227183064107	20.975510258022513
22-23	21.65550310169072	27.659264215529955	29.31889754279838	21.36633513998095
24-25	21.512005033400904	27.78518270866061	29.392819054142983	21.309993203795507
26-27	21.497114942540737	27.98357384469798	29.25641250526842	21.262898707492866
28-29	21.36902713758478	27.974929566640526	29.272082364674507	21.383960931100194
30-31	21.740191050619334	27.827614456133816	29.234622826976853	21.197571666270004
32-33	21.536675206271365	27.891517784795568	29.238774817555658	21.333032191377406
34-35	21.61836849904085	27.985905738265853	29.217167062688443	21.178558700004853
36-37	21.587771933832066	27.872798141447912	29.146434847455872	21.392995077264153
38-39	21.50381858658802	27.926169807426948	29.176496739696127	21.3935148662889
40-41	21.662138785534623	27.855067837275925	29.061080654692162	21.421712722497286
42-43	21.62074943626761	27.90595807994933	28.944648895213486	21.528643588569576
44-45	21.64447552271953	28.087600811509706	28.67894011640624	21.588983549364528
46-47	21.769845760653478	27.96036340311052	28.365901225332063	21.903889610903935
48-49	21.772033844792674	28.12565986612967	28.29165848210218	21.810647806975478
50-51	21.66941661638459	28.216502658851883	28.25399670006034	21.86008402470319
52-53	21.781269715435695	28.19213680047285	28.19845789584761	21.828135588243846
54-55	21.757150525988642	28.134115331368136	28.15911980486151	21.94961433778171
56-57	21.848456250437287	28.222013009519664	28.044781349256116	21.884749390786933
58-59	21.72211597633544	28.40955540812097	28.175761262444343	21.69256735309925
60-61	21.930127614130406	28.40958483164559	27.971131349779	21.689156204445005
62-63	21.80579996977659	28.52110032709529	27.91041683931383	21.76268286381429
64-65	21.770859876318653	28.440675975605906	27.934008787862037	21.854455360213404
66-67	21.795588088647456	28.45342760198333	27.847311777019517	21.903672532349695
68-69	21.82707097274905	28.306734301932167	27.932027202242004	21.93416752307678
70-71	21.827839576157153	28.146561761737203	27.957192181491692	22.06840648061395
72-73	21.85277203533943	28.151041409695978	27.920780253467665	22.075406301496926
74-75	21.837551745309984	28.1837680547025	27.86730016336005	22.111380036627466
76-77	21.943984225877372	28.130061966767673	27.81354533485363	22.112408472501325
78-79	21.853296905040754	28.127505677079633	27.839929259651697	22.179268158227917
80-81	21.882622875331453	28.098207546394434	27.844391263958247	22.17477831431587
82-83	21.869084052959906	28.153082354033813	27.92094545204218	22.0568881409641
84-85	22.004032272271637	28.11482077517577	27.872831588386283	22.008315364166307
86-87	21.900638979792184	28.06223580954296	27.926101669917692	22.111023540747173
88-89	21.921532016225466	28.027663667447513	27.932041665820257	22.118762650506763
90-91	21.94517563399835	28.087970774134764	27.970774134764348	21.996079457102535
92-93	22.032946767616956	27.978174289345148	28.009293353235957	21.979585589801935
94-95	21.898376610295443	27.995970997148362	28.078168624214904	22.027483768341288
96-97	21.753951269502046	28.02870886372825	28.210972985617293	22.00636688115241
98-99	21.92317393414058	28.532319840476717	28.253460140814763	21.291046084567938
100-101	20.514857921075063	32.059587575572095	26.08717242205836	21.338382081294487
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2272.0
1	2014.5
2	2738.5
3	4000.0
4	5467.0
5	8342.5
6	10654.5
7	12697.5
8	14376.0
9	14589.5
10	13663.5
11	12853.5
12	14743.0
13	20705.5
14	34230.5
15	52523.0
16	67258.5
17	74801.0
18	75081.0
19	70817.5
20	64867.0
21	59614.0
22	57823.0
23	62220.5
24	73413.0
25	90546.5
26	111594.5
27	135767.5
28	162313.5
29	190273.0
30	217948.0
31	244838.5
32	272216.5
33	298682.0
34	325084.5
35	349830.0
36	373949.0
37	395290.0
38	410729.5
39	425080.0
40	440334.0
41	453983.0
42	461587.5
43	467651.5
44	474382.5
45	478151.0
46	482285.5
47	483102.0
48	481707.5
49	470659.0
50	451009.0
51	432709.0
52	408774.5
53	380815.0
54	352645.5
55	322616.5
56	291650.5
57	260448.5
58	228280.5
59	194406.0
60	158980.5
61	127066.5
62	99654.5
63	76107.5
64	56447.0
65	41690.5
66	30976.5
67	23103.5
68	17240.0
69	12459.5
70	8864.5
71	6315.0
72	4499.5
73	3049.0
74	2000.5
75	1269.0
76	797.5
77	525.0
78	336.5
79	221.5
80	158.0
81	114.0
82	86.5
83	57.0
84	33.0
85	22.0
86	18.5
87	18.5
88	16.5
89	11.0
90	7.0
91	4.5
92	2.5
93	2.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.738832262850231E-5
2	0.016058115947421885
3	1.738832262850231E-5
4	1.3041241971376735E-4
5	1.738832262850231E-5
6	1.738832262850231E-5
7	1.738832262850231E-5
8	0.0
9	0.0
10-11	1.4780074234226965E-4
12-13	6.129383726547065E-4
14-15	3.042956459987905E-4
16-17	0.0016823202143075987
18-19	4.347080657125578E-6
20-21	0.0
22-23	2.652032548178084E-4
24-25	0.009340212275021768
26-27	0.01997771525661969
28-29	0.34595728054418806
30-31	0.3221704580941652
32-33	0.3161030770409969
34-35	0.18498335259258372
36-37	0.04430279465808505
38-39	0.035991590412872684
40-41	0.014438332844348013
42-43	0.012972043924406432
44-45	0.016653399187713296
46-47	0.010860585598945566
48-49	0.006369287223375504
50-51	0.003494062691929838
52-53	0.0012950597211881353
54-55	7.534298954201633E-5
56-57	2.230936163945138E-4
58-59	0.0010867570609167548
60-61	9.322852257572149E-4
62-63	2.486112987205634E-4
64-65	7.383015382955531E-5
66-67	0.0034681757062402434
68-69	0.005771126385504299
70-71	0.007114330081319997
72-73	0.010822434073339103
74-75	0.007930025084741433
76-77	0.0060262252082249786
78-79	0.0038005886347431024
80-81	0.0048828067297001735
82-83	0.0017563163770684023
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0026672776878657375
92-93	0.015100822711075684
94-95	0.0013668578397414693
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	188.0
20-21	816.0
22-23	1537.0
24-25	3204.0
26-27	4616.0
28-29	8326.0
30-31	21733.0
32-33	29028.0
34-35	42671.0
36-37	58430.0
38-39	68972.0
40-41	75340.0
42-43	88775.0
44-45	105228.0
46-47	104667.0
48-49	91811.0
50-51	83071.0
52-53	74957.0
54-55	82175.0
56-57	89493.0
58-59	92610.0
60-61	94244.0
62-63	96113.0
64-65	106277.0
66-67	113498.0
68-69	106055.0
70-71	103086.0
72-73	101482.0
74-75	103060.0
76-77	103687.0
78-79	110533.0
80-81	112746.0
82-83	112729.0
84-85	116831.0
86-87	124444.0
88-89	135534.0
90-91	149411.0
92-93	161147.0
94-95	193969.0
96-97	296142.0
98-99	1097677.0
100-101	6835659.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.75577242364432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.92093519548409	74.4764161193059
2	7.52523923294191	12.605644492555246
3	1.9548516670061764	4.911903340312531
4	0.7393454543742339	2.4769779847609694
5	0.3322190114607241	1.3912629959356244
6	0.1851587427423009	0.9304868111623353
7	0.10126282168789161	0.5936942093786985
8	0.06287870318720082	0.42131634835528564
9	0.042326980356866974	0.3190616040734832
>10	0.1331553768968472	1.6702741146187507
>50	0.001989536575836574	0.10303168490610821
>100	6.026193394616309E-4	0.08096062350003984
>500	3.46579464359251E-5	0.01896967113502561
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.694161314251157E-6
2	0.0	1.7388322628502314E-5	0.0	0.0	8.694161314251157E-6
3	0.0	1.7388322628502314E-5	0.0	0.0	8.694161314251157E-6
4	0.0	3.477664525700463E-5	0.0	1.7388322628502314E-5	8.694161314251157E-6
5	0.0	4.3470806571255784E-5	0.0	2.6082483942753467E-5	8.694161314251157E-6
6	1.1302409708526503E-4	4.3470806571255784E-5	0.0	5.2164967885506935E-5	8.694161314251157E-6
7	1.1302409708526503E-4	5.2164967885506935E-5	0.0	5.2164967885506935E-5	1.7388322628502314E-5
8	1.1302409708526503E-4	6.085912919975809E-5	0.0	5.2164967885506935E-5	1.7388322628502314E-5
9	1.1302409708526503E-4	1.391065810280185E-4	0.0	7.82474518282604E-5	1.7388322628502314E-5
10-11	1.2171825839951618E-4	1.391065810280185E-4	0.0	1.2606533905664178E-4	1.7388322628502314E-5
12-13	1.2171825839951618E-4	1.391065810280185E-4	0.0	2.2604819417053004E-4	2.173540328562789E-5
14-15	1.4780074234226965E-4	1.782303069421487E-4	0.0	2.8256024271316256E-4	3.91237259141302E-5
16-17	2.3474235548478123E-4	1.8257738759927427E-4	0.0	4.6948471096956246E-4	5.651204854263251E-5
18-19	2.5213067811328356E-4	1.8692446825639984E-4	0.0	7.085741471114693E-4	7.390037117113482E-5
20-21	2.738660813989114E-4	1.999657102277766E-4	0.0	0.0012302238259665386	9.563577445676272E-5
22-23	2.956014846845393E-4	2.5213067811328356E-4	0.0	0.002060516231477524	9.998285511388829E-5
24-25	3.173368879701672E-4	2.78213162056037E-4	0.0	0.003464623283729086	1.0432993577101387E-4
26-27	3.477664525700463E-4	2.956014846845393E-4	0.0	0.0062858786302035854	1.0432993577101387E-4
28-29	3.564606138842974E-4	2.956014846845393E-4	0.0	0.021052911622459175	1.0867701642813945E-4
30-31	3.564606138842974E-4	3.3037812994154395E-4	0.0	0.028908086369885094	1.1302409708526503E-4
32-33	3.6950185585567414E-4	3.477664525700463E-4	0.0	0.03263788157369884	1.1302409708526503E-4
34-35	3.868901784841764E-4	3.8689017848417647E-4	0.0	0.037558776877564995	1.1302409708526503E-4
36-37	4.0862558176980434E-4	4.3036098505543226E-4	0.0	0.04510965597899212	1.1302409708526503E-4
38-39	4.0862558176980434E-4	4.43402227026809E-4	0.0	0.05892467830733721	1.3041241971376735E-4
40-41	4.5209638834106013E-4	5.042613562265671E-4	0.0	0.08676338283556942	1.3041241971376735E-4
42-43	4.6079054965531127E-4	5.216496788550694E-4	0.0	0.10019151498543033	1.391065810280185E-4
44-45	4.6948471096956246E-4	5.825088080548275E-4	0.0	0.11914043956984072	1.5214782299939524E-4
46-47	4.6948471096956246E-4	6.3902085659746E-4	0.0	0.14291897076431764	1.564949036565208E-4
48-49	4.6948471096956246E-4	6.477150179117111E-4	0.0	0.16194179571989914	1.564949036565208E-4
50-51	4.8687303359806473E-4	7.303095503970971E-4	0.0	0.18505522357383586	1.6518906497077197E-4
52-53	5.042613562265671E-4	7.303095503970971E-4	0.0	0.21149851521113075	1.6518906497077197E-4
54-55	5.042613562265671E-4	7.52044953682725E-4	0.0	0.28639871493340446	1.6518906497077197E-4
56-57	5.042613562265671E-4	7.737803569683529E-4	0.0	0.353261162520653	1.6953614562789757E-4
58-59	5.216496788550694E-4	7.82474518282604E-4	0.0	0.4036351331754242	1.7388322628502314E-4
60-61	5.56426324112074E-4	8.085570022253575E-4	0.0	0.43023057263571846	1.8257738759927427E-4
62-63	5.86855888711953E-4	8.302924055109854E-4	0.0	0.4460930699535697	1.8692446825639984E-4
64-65	5.955500500262041E-4	9.215810993106225E-4	0.0	0.45775194027598054	1.9127154891352544E-4
66-67	6.259796146260832E-4	9.259281799677481E-4	0.0	0.4649289704408948	1.9127154891352544E-4
68-69	6.346737759403344E-4	9.607048252247527E-4	0.0	0.4720190589926666	2.173540328562789E-4
70-71	6.564091792259622E-4	9.78093147853255E-4	0.0	0.4783527555100986	2.390894361419068E-4
72-73	6.781445825115902E-4	9.867873091675063E-4	0.0	0.4824955233763393	2.4343651679903237E-4
74-75	6.781445825115902E-4	0.001030258115738762	0.0	0.48629921895132416	2.564777587704091E-4
76-77	6.781445825115902E-4	0.0010693818416528922	0.0	0.48962908273468236	2.608248394275347E-4
78-79	6.781445825115902E-4	0.0010867701642813946	0.0	0.49258075050087063	2.608248394275347E-4
80-81	6.868387438258414E-4	0.0010911172449385202	0.0	0.4937979330848658	2.738660813989114E-4
82-83	6.868387438258414E-4	0.0010954643255956457	0.0	0.494289153199121	2.8690732337028816E-4
84-85	6.955329051400926E-4	0.0010998114062527712	0.0	0.4946760433776052	2.8690732337028816E-4
86-87	6.998799857972181E-4	0.0011389351321669015	0.0	0.49483688536191883	2.999485653416649E-4
88-89	7.042270664543436E-4	0.0011824059387381572	0.0	0.49489774449111856	3.2603104928441835E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15695	0.0	48.062637	1
GTATCAA	28245	0.0	35.589405	1
TCAACGC	33880	0.0	29.136368	4
ATCAACG	33930	0.0	28.980473	3
CAACGCA	34980	0.0	28.232306	5
TATCAAC	36040	0.0	27.679132	2
AACGCAG	35860	0.0	27.575233	6
ACGCAGA	41220	0.0	23.979185	7
CGCAGAG	41420	0.0	23.832558	8
GCAGAGT	47690	0.0	20.529705	9
GTACATG	31755	0.0	20.066418	1
GAGTACT	28660	0.0	19.532944	12-13
TACATGG	31870	0.0	19.152016	2
AGAGTAC	42675	0.0	18.911198	10-11
ACATGGG	32130	0.0	18.489658	3
CAGAGTA	46270	0.0	18.449724	10-11
GTACTTT	32595	0.0	17.110155	14-15
AGTACTT	31615	0.0	16.986551	12-13
GTGGTAT	8225	0.0	16.41624	1
CATGGGG	22945	0.0	16.239975	4
>>END_MODULE