FastQCFastQC Report
Fri 27 May 2016
SRR2139077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9469356
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177800.048.494911
GTATCAA324700.036.2147871
ATCAACG400350.028.9990733
TCAACGC402150.028.8474484
TATCAAC409750.028.475382
CAACGCA408650.028.2763275
AACGCAG416650.027.8335746
ACGCAGA473350.024.4207677
CGCAGAG478850.024.1405288
GCAGAGT532200.021.5970429
GTGGTAT89550.021.1912081
GTACATG361250.020.6796741
TACATGG353450.020.667132
GAGTACT313200.020.36642812-13
TGGTATC91200.019.990042
ACATGGG363250.019.5734563
CAGAGTA509850.019.24835810-11
AGAGTAC477000.019.032110-11
GTACTTT344600.018.3479214-15
CATGGGG276950.017.9521874
Produced by FastQC (version 0.11.5)