##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139077_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9469356
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64586092232671	33.0	33.0	33.0	33.0	33.0
2	32.644935199394766	33.0	33.0	33.0	33.0	33.0
3	32.697690846135686	33.0	33.0	33.0	33.0	33.0
4	36.6208331379663	37.0	37.0	37.0	37.0	37.0
5	36.6284053530145	37.0	37.0	37.0	37.0	37.0
6	36.202685694782204	37.0	37.0	37.0	33.0	37.0
7	36.06247457588457	37.0	37.0	37.0	33.0	37.0
8	35.95867976660715	37.0	37.0	37.0	33.0	37.0
9	35.95156333757016	37.0	37.0	37.0	33.0	37.0
10-11	35.89239431910681	37.0	37.0	37.0	33.0	37.0
12-13	35.794916095666906	37.0	37.0	37.0	33.0	37.0
14-15	37.485738470493665	40.0	37.0	40.0	33.0	40.0
16-17	37.42039949707245	40.0	37.0	40.0	33.0	40.0
18-19	37.4689543829591	40.0	37.0	40.0	33.0	40.0
20-21	37.39982921436254	40.0	37.0	40.0	33.0	40.0
22-23	37.27655766550147	40.0	37.0	40.0	33.0	40.0
24-25	37.26095125207165	40.0	37.0	40.0	33.0	40.0
26-27	37.10396190195559	40.0	37.0	40.0	33.0	40.0
28-29	36.93373715668075	40.0	37.0	40.0	33.0	40.0
30-31	36.730858621266634	40.0	37.0	40.0	33.0	40.0
32-33	36.517876280587856	40.0	37.0	40.0	33.0	40.0
34-35	36.419448565510564	40.0	37.0	40.0	33.0	40.0
36-37	36.21178798468888	40.0	37.0	40.0	30.0	40.0
38-39	36.24704721391249	40.0	37.0	40.0	30.0	40.0
40-41	36.28600005326922	38.5	37.0	40.0	33.0	40.0
42-43	36.19987226558409	37.0	37.0	40.0	30.0	40.0
44-45	36.05957640283343	37.0	37.0	40.0	27.0	40.0
46-47	35.88043458295044	37.0	37.0	40.0	27.0	40.0
48-49	35.68340330478766	37.0	37.0	40.0	27.0	40.0
50-51	35.676651004083205	37.0	37.0	40.0	27.0	40.0
52-53	35.56005657885812	37.0	35.0	40.0	27.0	40.0
54-55	35.374475850999914	37.0	33.0	40.0	27.0	40.0
56-57	35.11977363384439	37.0	33.0	40.0	27.0	40.0
58-59	34.98940532433643	37.0	33.0	40.0	27.0	40.0
60-61	34.863933326131544	37.0	33.0	40.0	27.0	40.0
62-63	34.69534030293524	37.0	33.0	40.0	27.0	40.0
64-65	34.525096784995625	37.0	33.0	40.0	27.0	40.0
66-67	34.269830519676795	37.0	33.0	38.5	27.0	40.0
68-69	34.072830013667726	37.0	33.0	37.0	27.0	40.0
70-71	33.85010600986767	37.0	33.0	37.0	27.0	40.0
72-73	33.55846774687586	37.0	33.0	37.0	27.0	40.0
74-75	33.21298415613883	37.0	33.0	37.0	22.0	38.5
76-77	31.155710373024768	33.0	30.0	35.0	22.0	37.0
78-79	33.26811965645963	35.0	33.0	37.0	27.0	37.0
80-81	33.634734120019786	37.0	33.0	37.0	27.0	37.0
82-83	33.58345747153923	37.0	33.0	37.0	27.0	37.0
84-85	33.47925696734352	37.0	33.0	37.0	27.0	37.0
86-87	33.34721992719282	37.0	33.0	37.0	27.0	37.0
88-89	33.1897592286035	37.0	33.0	37.0	27.0	37.0
90-91	33.118268681767475	33.0	33.0	37.0	27.0	37.0
92-93	33.165324854499886	33.0	33.0	37.0	27.0	37.0
94-95	33.227697336728546	33.0	33.0	37.0	27.0	37.0
96-97	33.34859367716176	33.0	33.0	37.0	27.0	37.0
98-99	33.4968758079332	33.0	33.0	37.0	27.0	37.0
100	33.47152871249446	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	7.0
15	14.0
16	50.0
17	161.0
18	472.0
19	1256.0
20	3063.0
21	7647.0
22	18704.0
23	41003.0
24	77506.0
25	126300.0
26	182701.0
27	239539.0
28	288637.0
29	325604.0
30	349322.0
31	370233.0
32	397425.0
33	443728.0
34	530143.0
35	698018.0
36	1123088.0
37	2316363.0
38	1887877.0
39	40494.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57996003107286	20.027507678452476	14.533512099450057	22.859020191024605
2	14.82305272671168	21.022126408681174	41.57702803940052	22.57779282520663
3	18.150640084654654	26.663597020189968	30.5494209562512	24.636341938904177
4	12.655325895277644	18.07994371505557	38.539567483743205	30.725162905923586
5	13.372253270920066	38.223029786403586	34.907059129904745	13.4976578127716
6	30.12706460713907	37.59090903330702	18.56522238682335	13.716803972730563
7	26.79280407242055	33.30434508957104	22.48047280089586	17.422378037112555
8	25.32008512511305	35.97686051723053	20.808732927561284	17.89432143009514
9	26.561806315022903	16.680807015809734	21.0119357641639	35.745450905003466
10-11	24.71026901598099	26.750742884050737	28.219345066598855	20.319643033369417
12-13	25.713839907679564	24.573410034699368	28.468145515047876	21.244604542573192
14-15	22.832129060329116	25.662911630087887	26.66257263128547	24.84238667829753
16-17	21.871645759225867	28.814446304479418	27.1392162254751	22.174691710819616
18-19	21.92057200088369	27.63775065590522	29.438453892746242	21.003223450464848
20-21	23.004282298862723	26.623683411045214	29.35796161517429	21.014072674917774
22-23	22.68812406366816	26.67347922150501	29.16558490650657	21.472811808320262
24-25	22.412037801092797	26.897628501957872	29.241240960886884	21.449092736062454
26-27	22.310401940936465	27.04783124376225	29.166940272642673	21.47482654265862
28-29	22.152753125749932	27.133389521764535	29.14180735028382	21.57205000220171
30-31	22.569254277448074	26.978256938040225	28.999374933998247	21.453113850513457
32-33	22.370594795664722	27.16448151647861	28.940793171441214	21.524130516415447
34-35	22.331902242928443	27.249259522345266	28.946426110026213	21.47241212470008
36-37	22.506252378430673	27.128674441548	28.763272442977584	21.601800737043742
38-39	22.323386768717015	27.272341599658862	28.79376682920126	21.61050480242286
40-41	22.537138916985224	27.230524082661674	28.58141769241759	21.650919307935514
42-43	22.473644891853333	27.2878352525091	28.39182849492084	21.846691360716722
44-45	22.545008760591188	27.47663131331241	28.009460624294803	21.9688993018016
46-47	22.653343883577254	27.43464934436562	27.611222008419077	22.30078476363805
48-49	22.625147834814832	27.57359521063017	27.50998696184598	22.29126999270902
50-51	22.470143350726055	27.75740914864846	27.365765783971636	22.40668171665385
52-53	22.69494258012772	27.713918762690742	27.13287679691552	22.458261860266013
54-55	22.701565517457684	27.59563083641136	27.204161110400527	22.49864253573043
56-57	22.682220331668653	27.745883017704593	27.11382571393588	22.45807093669088
58-59	22.52938372108901	27.93588153538069	27.214606694210413	22.320128049319887
60-61	22.697520698390232	27.857858223073823	27.160609874682457	22.284011203853495
62-63	22.61630629278277	28.007641280283373	27.009117112309234	22.366935314624623
64-65	22.613676090169825	28.03191695110476	27.052004125215458	22.302402833509962
66-67	22.710934559899645	27.92651133257447	26.91842869421795	22.444125413307937
68-69	22.59835287505573	27.83768198614091	27.05303578621133	22.510929352592036
70-71	22.614059859284588	27.669764471199816	27.05694390303055	22.659231766485046
72-73	22.764945872676957	27.61062453261373	26.973991353329975	22.65043824137934
74-75	22.570065355639247	27.786804990747132	26.936032566148775	22.70709708746485
76-77	22.686237264359164	27.633243151167452	26.924639670358125	22.75587991411526
78-79	22.641196262641405	27.60387009033257	26.924783783556805	22.83014986346922
80-81	22.65282985131038	27.61947379254761	26.988869893035233	22.73882646310678
82-83	22.708596188354097	27.575041112488563	27.05432252980668	22.662040169350657
84-85	22.873302020825882	27.55191830880102	26.994547563813125	22.580232106559965
86-87	22.619317640267777	27.63043241918812	27.063606561448122	22.686643379095976
88-89	22.722155323402905	27.553407610862756	27.038029784359587	22.686407281374752
90-91	22.77612731161621	27.553737454036902	27.08973152981423	22.58040370453265
92-93	22.81735032048635	27.578540561825676	27.08662191423576	22.517487203452212
94-95	22.659222012541857	27.567058847126404	27.18731504648234	22.586404093849403
96-97	22.69502634131208	27.622344698650718	27.334585406637213	22.34804355339999
98-99	23.97117935111151	29.555609814442708	27.363497774660644	19.109713059785143
100	23.147859483695076	30.095382574255993	21.189525935849627	25.567232006199305
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2053.0
1	2014.5
2	2970.5
3	4214.5
4	6264.0
5	11469.0
6	15791.0
7	17572.0
8	17514.5
9	15776.0
10	13750.0
11	12182.5
12	13307.0
13	19492.0
14	33956.0
15	54361.5
16	72572.0
17	84237.5
18	87920.5
19	82915.0
20	73737.5
21	64057.5
22	56876.5
23	55792.0
24	60478.0
25	70351.0
26	84197.0
27	100647.5
28	116456.0
29	133280.0
30	150792.5
31	168121.5
32	189481.0
33	210592.5
34	231130.0
35	250629.0
36	271175.0
37	289852.5
38	301168.0
39	311531.5
40	324957.0
41	337340.5
42	345531.5
43	354194.0
44	363407.5
45	372668.0
46	380360.5
47	383521.0
48	387710.0
49	390037.5
50	387304.0
51	383751.0
52	376946.0
53	364656.0
54	349730.5
55	330299.0
56	306164.0
57	279428.5
58	252271.0
59	225114.5
60	194944.0
61	163842.5
62	133210.0
63	105683.5
64	82476.0
65	64373.5
66	50633.0
67	40014.5
68	31706.5
69	23992.0
70	17849.5
71	13708.5
72	10452.0
73	7830.0
74	5746.5
75	4232.5
76	3135.0
77	2341.5
78	1746.0
79	1272.5
80	918.5
81	661.5
82	499.0
83	348.0
84	247.0
85	195.0
86	158.5
87	124.0
88	90.0
89	62.5
90	36.5
91	22.0
92	17.5
93	9.5
94	4.0
95	4.0
96	4.0
97	4.0
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.0129444916845456E-4
3	0.0031258725514174356
4	0.004160789815062398
5	2.112076048255024E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.2144437277466386E-4
12-13	0.0017266221694484819
14-15	0.0031258725514174356
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.288702886383642E-6
24-25	0.006837194017693557
26-27	0.020677415703043014
28-29	0.009418280365755111
30-31	0.0
32-33	0.0
34-35	1.833529538969783E-4
36-37	0.014654787054233285
38-39	0.03940392313504132
40-41	0.04506670892927356
42-43	0.00796780212267332
44-45	2.955599863529359E-4
46-47	5.636616679458968E-6
48-49	0.036504202763291325
50-51	0.054963350020487176
52-53	0.0534210805622401
54-55	0.029827914265319475
56-57	0.0013526175030586798
58-59	0.006358127875071513
60-61	0.028880167607987242
62-63	0.035676492746233246
64-65	0.018029442427731933
66-67	0.047485203674930004
68-69	0.0634246553860147
70-71	0.07366827402060276
72-73	0.05040643150675094
74-75	0.022581835340125204
76-77	0.005187558061186854
78-79	5.290196716626182E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0022632479886677843
92-93	0.002719807346473143
94-95	6.032866271960515E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3235.0
20-21	9706.0
22-23	10611.0
24-25	19031.0
26-27	24911.0
28-29	35896.0
30-31	39433.0
32-33	42304.0
34-35	49247.0
36-37	49854.0
38-39	52028.0
40-41	65484.0
42-43	78502.0
44-45	92871.0
46-47	91654.0
48-49	69351.0
50-51	74659.0
52-53	63499.0
54-55	73693.0
56-57	78820.0
58-59	79139.0
60-61	77484.0
62-63	82246.0
64-65	99053.0
66-67	108650.0
68-69	92705.0
70-71	88546.0
72-73	79380.0
74-75	86734.0
76-77	65485.0
78-79	88675.0
80-81	101659.0
82-83	128506.0
84-85	142067.0
86-87	153037.0
88-89	166539.0
90-91	177410.0
92-93	190175.0
94-95	221675.0
96-97	393268.0
98-99	577675.0
100-101	5244459.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.12993915866426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.76597485612866	77.26901917179082
2	6.046621305011738	10.294970076222645
3	1.6525053983457165	4.220330520616052
4	0.668689509375887	2.2770198899682526
5	0.32746702681138196	1.3938624034460811
6	0.17560833479139795	0.8969716113927628
7	0.10126953564877483	0.6034748585884466
8	0.06458279852280124	0.4398343767153872
9	0.04442303569940466	0.340355729370014
>10	0.14920655590672624	1.962763449477227
>50	0.002734561195888894	0.15352420408755807
>100	8.740083278044937E-4	0.1155499270037329
>500	2.1537129175128107E-5	0.013439221458105853
>1k	2.1537104712925448E-5	0.018884559862810783
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.392266168892583E-5	0.0	0.0	0.0
3	0.0	8.448304193020095E-5	0.0	2.112076048255024E-5	0.0
4	0.0	1.7952646410167704E-4	0.0	2.112076048255024E-5	0.0
5	0.0	1.9008684434295215E-4	0.0	2.112076048255024E-5	0.0
6	0.0	2.0064722458422728E-4	0.0	2.112076048255024E-5	0.0
7	0.0	2.1120760482550238E-4	0.0	4.224152096510048E-5	0.0
8	0.0	2.4288874554932776E-4	0.0	6.336228144765072E-5	0.0
9	0.0	5.385793923050311E-4	0.0	6.336228144765072E-5	0.0
10-11	0.0	5.385793923050311E-4	0.0	7.392266168892583E-5	0.0
12-13	0.0	5.491397725463063E-4	0.0	1.0032361229211364E-4	0.0
14-15	0.0	7.128256662860706E-4	0.0	1.7952646410167704E-4	0.0
16-17	0.0	7.181058564067082E-4	0.0	3.168114072382536E-4	0.0
18-19	0.0	7.814681378543588E-4	0.0	6.23062434235232E-4	0.0
20-21	0.0	8.501106094226472E-4	0.0	0.0010243568834036865	0.0
22-23	0.0	9.24033271111573E-4	0.0	0.001668540078121469	0.0
24-25	0.0	9.926757426798613E-4	0.0	0.0030255489391253216	0.0
26-27	0.0	0.0010349172636449618	0.0	0.00506370232569142	0.0
28-29	1.056038024127512E-5	0.0010349172636449618	0.0	0.016020096826014355	0.0
30-31	2.112076048255024E-5	0.0012725258190736518	0.0	0.024811613376875895	0.0
32-33	2.112076048255024E-5	0.0012936465795562021	0.0	0.033782656391839104	0.0
34-35	2.112076048255024E-5	0.0014784532337785167	0.0	0.044828814124212885	0.0
36-37	3.168114072382536E-5	0.0016474193176389186	0.0	0.062316803803764476	0.0
38-39	4.224152096510048E-5	0.0017002212188452941	0.0	0.09537607414907623	0.0
40-41	4.224152096510048E-5	0.0018375061619818709	0.0	0.13319807598320307	0.0
42-43	4.224152096510048E-5	0.0019008684434295216	0.0	0.1672183409304709	0.0
44-45	5.2801901206375596E-5	0.0021331968087375743	0.0	0.20929089581171095	0.0
46-47	5.2801901206375596E-5	0.0023338440333218013	0.0	0.25293694734890104	0.0
48-49	5.2801901206375596E-5	0.002349684603683714	0.0	0.29430195675397564	0.0
50-51	5.2801901206375596E-5	0.002629534680077505	0.0	0.33418323273515116	0.0
52-53	5.2801901206375596E-5	0.002650655440560055	0.0	0.4035068488290017	0.0
54-55	6.336228144765072E-5	0.0026664960109219677	0.0	0.4852283513261092	0.0
56-57	6.336228144765072E-5	0.0027245781022489806	0.0	0.5709047162235743	0.0
58-59	6.336228144765072E-5	0.0027562592429728064	0.0	0.638126816649411	0.0
60-61	6.336228144765072E-5	0.002824901714541094	0.0	0.6871586621096514	0.0
62-63	6.336228144765072E-5	0.0028618630453855574	0.0	0.7314858581724037	0.0
64-65	6.336228144765072E-5	0.0032314763538301866	0.0	0.7457265309277632	0.0
66-67	7.392266168892583E-5	0.003263157494554012	0.0	0.7544599653872976	0.0
68-69	7.392266168892583E-5	0.0033001188253984747	0.0	0.7628818686297146	0.0
70-71	7.392266168892583E-5	0.0033317999661223	0.0	0.768325744644092	0.0
72-73	7.392266168892583E-5	0.0033529207266048506	0.0	0.774487726514876	0.0
74-75	7.92028518095634E-5	0.003442683958655689	0.0	0.780575785723971	0.0
76-77	8.448304193020095E-5	0.0035641283314303525	0.0	0.7854758021559227	0.0
78-79	8.448304193020095E-5	0.003622210422757366	0.0	0.7882954236803432	0.0
80-81	8.448304193020095E-5	0.003622210422757366	0.0	0.7892247371415755	0.0
82-83	8.448304193020095E-5	0.0036274906128780033	0.0	0.791299851858986	0.0
84-85	8.448304193020095E-5	0.003632770802998641	0.0	0.7930211938383138	0.0
86-87	9.504342217147607E-5	0.0037489349856526678	0.0	0.7941458743340095	0.0
88	9.504342217147607E-5	0.0039179010695130695	0.0	0.7942356375660604	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17780	0.0	48.49491	1
GTATCAA	32470	0.0	36.214787	1
ATCAACG	40035	0.0	28.999073	3
TCAACGC	40215	0.0	28.847448	4
TATCAAC	40975	0.0	28.47538	2
CAACGCA	40865	0.0	28.276327	5
AACGCAG	41665	0.0	27.833574	6
ACGCAGA	47335	0.0	24.420767	7
CGCAGAG	47885	0.0	24.140528	8
GCAGAGT	53220	0.0	21.597042	9
GTGGTAT	8955	0.0	21.191208	1
GTACATG	36125	0.0	20.679674	1
TACATGG	35345	0.0	20.66713	2
GAGTACT	31320	0.0	20.366428	12-13
TGGTATC	9120	0.0	19.99004	2
ACATGGG	36325	0.0	19.573456	3
CAGAGTA	50985	0.0	19.248358	10-11
AGAGTAC	47700	0.0	19.0321	10-11
GTACTTT	34460	0.0	18.34792	14-15
CATGGGG	27695	0.0	17.952187	4
>>END_MODULE