FastQCFastQC Report
Fri 27 May 2016
SRR2139076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11385978
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA179750.051.595451
GTATCAA331900.037.4549751
ATCAACG412650.029.654763
TCAACGC432000.028.4042514
TATCAAC439950.027.9222242
CAACGCA440600.027.8498335
AACGCAG444000.027.6746486
ACGCAGA450450.027.2876687
CGCAGAG518450.023.7086128
GCAGAGT590350.020.7364469
GTACATG387400.019.8327541
GAGTACT353550.019.62764712-13
GTGGTAT87500.019.5235441
TGGTATC83850.019.424052
TACATGG388700.019.2056962
AGAGTAC543100.019.179710-11
ACATGGG382650.019.1571923
CAGAGTA576650.018.41956910-11
AGTACTT378550.018.13786512-13
CTTATAC114100.018.0178261
Produced by FastQC (version 0.11.5)