##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139076_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11385978
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66220758550561	34.0	31.0	34.0	31.0	34.0
2	32.778054111820694	34.0	31.0	34.0	31.0	34.0
3	33.409805376402446	34.0	31.0	34.0	31.0	37.0
4	36.190695959539006	37.0	35.0	37.0	35.0	37.0
5	36.1642238374253	37.0	35.0	37.0	35.0	37.0
6	36.204600079149984	37.0	37.0	37.0	35.0	37.0
7	36.21479349424354	37.0	37.0	37.0	35.0	37.0
8	36.47917456014758	37.0	37.0	37.0	35.0	39.0
9	37.844692304868325	39.0	38.0	39.0	35.0	39.0
10-11	37.79415176280861	39.0	38.0	39.0	35.0	39.0
12-13	37.928352180199184	39.0	38.0	39.0	35.0	40.0
14-15	39.08152606653552	40.0	38.5	41.0	36.0	41.0
16-17	39.04059853268643	40.0	38.0	41.0	36.0	41.0
18-19	39.02062396396691	40.0	38.5	41.0	35.0	41.0
20-21	38.99743503599749	40.0	38.5	41.0	35.0	41.0
22-23	38.88292370158827	40.0	38.0	41.0	35.0	41.0
24-25	38.73468757168871	40.0	38.0	41.0	34.5	41.0
26-27	38.70275194389192	40.0	38.0	41.0	34.5	41.0
28-29	38.63730403393294	40.0	38.0	41.0	35.0	41.0
30-31	38.55441363077278	40.0	38.0	41.0	34.0	41.0
32-33	38.426612664907026	40.0	38.0	41.0	34.0	41.0
34-35	38.242652827593986	40.0	38.0	41.0	34.0	41.0
36-37	38.39204264422972	40.0	38.0	41.0	34.0	41.0
38-39	38.348669693248425	40.0	38.0	41.0	34.0	41.0
40-41	38.34067648705681	40.0	38.0	41.0	34.0	41.0
42-43	38.35637291718893	40.0	38.0	41.0	34.0	41.0
44-45	38.29650191398116	40.0	38.0	41.0	33.5	41.0
46-47	38.22695232977807	40.0	38.0	41.0	33.0	41.0
48-49	38.09461658574648	40.0	37.5	41.0	33.0	41.0
50-51	38.07666918621439	40.0	37.0	41.0	33.0	41.0
52-53	37.9761827040844	40.0	37.0	41.0	33.0	41.0
54-55	37.818395773781276	40.0	37.0	41.0	33.0	41.0
56-57	37.64240189272304	39.5	36.0	41.0	33.0	41.0
58-59	37.47721050010684	39.0	36.0	41.0	32.0	41.0
60-61	37.28033548805681	39.0	35.5	41.0	32.0	41.0
62-63	37.05475024508537	39.0	35.0	40.0	32.0	41.0
64-65	36.75138615138368	38.0	35.0	40.0	31.0	41.0
66-67	36.393223886418625	37.5	35.0	40.0	31.0	41.0
68-69	36.06348901567844	37.0	35.0	40.0	31.0	41.0
70-71	35.76159782802097	36.5	34.0	39.0	30.5	41.0
72-73	35.43085236376881	36.0	34.0	39.0	30.0	40.5
74-75	34.97932259778183	35.5	34.0	38.5	29.5	40.0
76-77	34.21810693589093	35.0	33.0	37.0	29.0	39.0
78-79	34.43669416381525	35.0	34.0	37.0	29.5	39.0
80-81	34.233106085295	35.0	34.0	36.5	29.5	39.0
82-83	33.972478302605026	35.0	33.5	36.0	29.0	37.5
84-85	33.740443307007354	35.0	33.0	36.0	29.0	37.0
86-87	33.50924124736899	35.0	33.0	35.0	29.0	37.0
88-89	33.285294054511596	35.0	33.0	35.0	29.0	36.0
90-91	33.12152569677677	35.0	33.0	35.0	29.0	36.0
92-93	33.0406123927428	35.0	33.0	35.0	29.0	36.0
94-95	32.97435968618581	34.0	32.5	35.0	29.0	35.5
96-97	32.97245967628664	34.0	32.0	35.0	29.0	35.0
98-99	33.04836863630911	34.0	32.0	35.0	29.0	35.0
100-101	30.876788009137904	32.5	28.0	34.5	23.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	4.0
19	14.0
20	79.0
21	375.0
22	1652.0
23	5482.0
24	14017.0
25	29011.0
26	51310.0
27	80042.0
28	113900.0
29	153965.0
30	198134.0
31	249227.0
32	316597.0
33	413645.0
34	575507.0
35	825983.0
36	1372411.0
37	2517176.0
38	2924049.0
39	1436088.0
40	107307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.787785666317745	0.006815642320712157	26.606879899072283	30.59851879228926
2	14.210952838713938	24.799518507119096	37.92351100504963	23.066017649117335
3	15.480307385344188	25.073570813340524	33.92595746571943	25.520164335595858
4	12.695073444787477	24.153870799566622	37.014700902022554	26.136354853623338
5	17.21850058677457	36.561088230132974	32.18695212197042	14.033459061122045
6	27.706290212853112	37.86034466616558	20.067180901695366	14.366184219285937
7	25.898293813072122	33.28874631190972	22.38420709550189	18.428752779516273
8	24.21605980760369	32.320897890448926	21.963007654064292	21.500034647883094
9	23.744852768427823	20.605692529625262	23.75030684680056	31.899147855146353
10-11	24.58635322780417	26.231428595894812	28.551221340507087	20.63099683579393
12-13	24.869289270274013	24.428587243837775	28.588284596476992	22.11383888941122
14-15	22.258973264392633	26.8135062070739	26.756993428858035	24.17052709967543
16-17	21.22284057033666	29.167467920462165	27.708396286374743	21.90129522282643
18-19	21.36419837877754	28.058231459472598	29.687648208638418	20.889921953111443
20-21	22.11840857313613	27.197031035521128	29.627429791625215	21.057130599717528
22-23	21.982989087025608	27.060205252145074	29.3949345675669	21.56187109326242
24-25	21.63225634334706	27.40113190450147	29.604704241926616	21.361907510224857
26-27	21.592646489060456	27.573282460540383	29.45485457408768	21.379216476311477
28-29	21.434356799936385	27.409900618383233	29.545676283877604	21.610066297802778
30-31	21.638688191919258	27.354455213511812	29.56915355154031	21.43770304302862
32-33	21.456423948535416	27.595645672513896	29.43834632290801	21.509584056042677
34-35	21.65282722619052	27.48271519809171	29.391344532474157	21.473113043243604
36-37	21.629478994399225	27.587337714235883	29.318697803953132	21.464485487411764
38-39	21.64125812914027	27.73602347262688	29.22845720914048	21.394261189092372
40-41	21.84836085443989	27.447019985299566	29.01929424263364	21.685324917626907
42-43	21.805557314861503	27.58745175717301	28.962063055610965	21.644927872354522
44-45	21.861223932029418	27.914090882763233	28.533655676767893	21.69102950843946
46-47	22.003062689828397	27.64210337621677	28.196051280488888	22.158782653465945
48-49	21.91944814980207	27.923403919002677	28.13196422691456	22.0251837042807
50-51	21.910670266591563	28.136843930494575	27.858101264504764	22.094384538409095
52-53	22.080231054204464	27.899730937352047	27.868678976911742	22.151359031531744
54-55	21.951540357066524	27.939439538863404	27.968499641546945	22.140520462523128
56-57	22.050787382269572	28.237962142319915	27.648801965509897	22.062448509900616
58-59	22.05159387456109	28.117636059537016	27.836016366266087	21.994753699635808
60-61	22.110856138337674	28.244661219397422	27.74724041809697	21.897242224167936
62-63	21.980414674441278	28.46033181093508	27.615123042303168	21.944130472320477
64-65	22.044002557632467	28.196698906350466	27.64708228448301	22.112216251534058
66-67	21.96422796635588	28.200235272907882	27.682227619056583	22.153309141679657
68-69	21.998622254411956	28.226674649554056	27.67501944449389	22.099683651540094
70-71	22.03695542699384	27.85208622395726	27.70310766070052	22.40785068834838
72-73	22.05077579324088	27.893951805876828	27.723495931263887	22.331776469618404
74-75	21.95302880997406	28.08799429881211	27.680749650144577	22.278227241069253
76-77	22.21321206016434	27.866932985868125	27.490964488148762	22.42889046581877
78-79	21.98485284113333	27.886406139009818	27.683389542126886	22.445351477729975
80-81	22.097153958636483	28.017781380374373	27.5633439576115	22.321720703377643
82-83	22.144882092255276	27.77763415285713	27.681242933086963	22.39624082180063
84-85	22.17023889167338	27.860613979608946	27.719595268253332	22.249551860464344
86-87	22.070413724794047	27.9291956773055	27.653013812591126	22.347376785309322
88-89	22.126444098744404	27.701315166908174	27.722769756076183	22.449470978271233
90-91	22.08587796526318	27.765954889642302	27.81037882702371	22.337788318070814
92-93	22.104713024266296	27.752737283194545	27.858908161576213	22.283641530962946
94-95	22.028503299158526	27.611840893754643	27.911103888749984	22.448551918336847
96-97	21.836315580531522	27.78726702660092	28.066922923737863	22.309494469129692
98-99	22.179933153070294	28.708397541907843	27.927358269474823	21.184311035547047
100-101	20.57865613722256	31.63372788148427	25.389584780727976	22.3980312005652
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2993.0
1	2661.0
2	3659.0
3	5254.5
4	7127.0
5	11103.5
6	14228.0
7	17073.0
8	19579.0
9	19774.0
10	18014.5
11	16050.0
12	17363.0
13	22938.5
14	38772.5
15	64044.0
16	86249.5
17	98002.5
18	98216.0
19	90761.0
20	80256.5
21	69582.5
22	62669.0
23	63449.5
24	71897.5
25	86868.5
26	106855.0
27	129003.5
28	153470.5
29	179208.5
30	204424.0
31	230242.5
32	256585.0
33	282342.0
34	308045.0
35	331278.0
36	355268.5
37	376978.0
38	391387.0
39	403206.5
40	415100.0
41	427579.5
42	435426.0
43	443191.0
44	454300.0
45	464087.0
46	473810.0
47	479505.5
48	477443.0
49	470364.0
50	461684.5
51	447042.5
52	424315.5
53	400624.5
54	376548.5
55	348803.0
56	320269.0
57	290735.0
58	258474.0
59	223054.5
60	185129.0
61	150620.0
62	119725.0
63	91715.5
64	68536.5
65	51811.5
66	39570.5
67	30002.5
68	23048.0
69	16799.0
70	11864.5
71	8745.5
72	6226.0
73	4300.0
74	2983.0
75	2020.5
76	1316.5
77	831.0
78	531.0
79	346.5
80	217.5
81	138.5
82	99.0
83	69.0
84	46.5
85	35.0
86	28.0
87	21.0
88	16.5
89	12.0
90	8.0
91	6.0
92	4.5
93	3.0
94	1.5
95	1.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0035482239645992643
2	0.012172867363699456
3	7.904459327077568E-5
4	8.782732585641742E-5
5	3.513093034256697E-5
6	3.513093034256697E-5
7	3.513093034256697E-5
8	8.782732585641743E-6
9	2.634819775692523E-5
10-11	2.2395968093386444E-4
12-13	4.698761933318332E-4
14-15	5.050071236744002E-4
16-17	0.0015501523013657674
18-19	1.7565465171283486E-5
20-21	7.026464696208824E-5
22-23	6.719712575494104E-4
24-25	0.011848215789177496
26-27	0.060243195808581965
28-29	0.3386189310050405
30-31	0.3197604769320752
32-33	0.3088485132153119
34-35	0.15749579339863035
36-37	0.04318936151949938
38-39	0.03431416139431729
40-41	0.016417474789079047
42-43	0.014615038163563963
44-45	0.017828905208088826
46-47	0.011561081357034327
48-49	0.00669721986723209
50-51	0.003998383150841928
52-53	0.0012610504339140285
54-55	9.66896509247374E-5
56-57	3.171426327034555E-4
58-59	0.001275796645108002
60-61	0.0010497988242153663
62-63	3.1166715435873804E-4
64-65	2.5911584795203983E-4
66-67	0.0042729254792540985
68-69	0.005303945484809505
70-71	0.007336465033080454
72-73	0.010347350761177656
74-75	0.007271504558381692
76-77	0.005296356510356695
78-79	0.0036669770711213515
80-81	0.004347022163938682
82-83	0.0014286695888629083
84-85	0.0
86-87	2.3319272200850755E-5
88-89	2.131834290625626E-4
90-91	0.0045843594426961095
92-93	0.013708482038849704
94-95	0.00114190081436978
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	266.0
20-21	905.0
22-23	1865.0
24-25	5115.0
26-27	6332.0
28-29	12813.0
30-31	27045.0
32-33	36221.0
34-35	53698.0
36-37	71031.0
38-39	81828.0
40-41	88634.0
42-43	107977.0
44-45	126470.0
46-47	122387.0
48-49	103595.0
50-51	90924.0
52-53	83677.0
54-55	92573.0
56-57	96846.0
58-59	100088.0
60-61	103110.0
62-63	103992.0
64-65	113301.0
66-67	120772.0
68-69	113143.0
70-71	110485.0
72-73	107734.0
74-75	109227.0
76-77	111143.0
78-79	116023.0
80-81	117948.0
82-83	117717.0
84-85	122713.0
86-87	130560.0
88-89	140677.0
90-91	152692.0
92-93	166840.0
94-95	204706.0
96-97	356965.0
98-99	1272733.0
100-101	6183207.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.03175072954743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.69937053956176	73.64864024508655
2	7.521742431425704	12.490868852359982
3	2.0327045417250376	5.063370504459967
4	0.7868908627598415	2.6134770387213475
5	0.37591898811846663	1.5606605857978113
6	0.19534691624828535	0.9731997873428044
7	0.10986878282414876	0.6385818171889452
8	0.07194949521190494	0.47792740412413254
9	0.04678195255227911	0.3495948680666119
>10	0.15630083361407382	1.9614551945249636
>50	0.0025786913434695056	0.14036983029020242
>100	5.273016534772244E-4	0.07354791752509483
>500	1.8662961643137606E-5	0.00830595451159065
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.782732585641743E-6
2	0.0	6.14791280994922E-5	0.0	0.0	8.782732585641743E-6
3	0.0	6.14791280994922E-5	0.0	0.0	8.782732585641743E-6
4	0.0	6.14791280994922E-5	0.0	0.0	8.782732585641743E-6
5	0.0	8.782732585641742E-5	0.0	0.0	8.782732585641743E-6
6	0.0	8.782732585641742E-5	0.0	8.782732585641743E-6	5.2696395513850456E-5
7	0.0	8.782732585641742E-5	0.0	8.782732585641743E-6	5.2696395513850456E-5
8	0.0	1.0539279102770091E-4	0.0	1.7565465171283486E-5	7.026186068513395E-5
9	0.0	1.4930645395590963E-4	0.0	3.513093034256697E-5	7.026186068513395E-5
10-11	8.782732585641743E-6	1.4930645395590963E-4	0.0	4.830502922102959E-5	7.904459327077568E-5
12-13	8.782732585641743E-6	1.5369782024873049E-4	0.0	6.14791280994922E-5	7.904459327077568E-5
14-15	8.782732585641743E-6	2.6787334386207316E-4	0.0	7.026186068513395E-5	1.3174098878462615E-4
16-17	8.782732585641743E-6	3.337438382543862E-4	0.0	1.1417552361334265E-4	1.5808918654155137E-4
18-19	8.782732585641743E-6	3.337438382543862E-4	0.0	3.8644023376823664E-4	1.5808918654155137E-4
20-21	8.782732585641743E-6	3.337438382543862E-4	0.0	9.090128226139204E-4	1.5808918654155137E-4
22-23	8.782732585641743E-6	3.337438382543862E-4	0.0	0.0014096285799954998	1.8004601800565573E-4
24-25	8.782732585641743E-6	3.6009203601131145E-4	0.0	0.0022483795419242863	2.0200284946976009E-4
26-27	8.782732585641743E-6	3.7765750118259493E-4	0.0	0.006367481124590263	2.2395968093386444E-4
28-29	8.782732585641743E-6	3.864402337682367E-4	0.0	0.023853901702602973	2.327424135195062E-4
30-31	8.782732585641743E-6	4.43527995574908E-4	0.0	0.03165735960494566	2.3713377981232707E-4
32-33	8.782732585641743E-6	4.479193618677289E-4	0.0	0.03456005272450026	2.634819775692523E-4
34-35	8.782732585641743E-6	5.357466877241463E-4	0.0	0.03795018750255797	2.72264710154894E-4
36-37	8.782732585641743E-6	6.455308450446682E-4	0.0	0.043926837027087176	2.72264710154894E-4
38-39	8.782732585641743E-6	6.938358742656977E-4	0.0	0.05542343398169222	2.8983017532617755E-4
40-41	8.782732585641743E-6	7.992286652933986E-4	0.0	0.07234775967422385	2.9861290791181926E-4
42-43	8.782732585641743E-6	9.002300900282786E-4	0.0	0.08186385043076669	3.0739564049746097E-4
44-45	8.782732585641743E-6	9.83666049591875E-4	0.0	0.09523116942611343	3.205697393759236E-4
46-47	8.782732585641743E-6	9.83666049591875E-4	0.0	0.11177783761746246	3.2935247196156536E-4
48-49	8.782732585641743E-6	9.92448782177517E-4	0.0	0.12637473917479902	3.381352045472071E-4
50-51	8.782732585641743E-6	0.0010758847417411135	0.0	0.14323758573923118	3.42526570840028E-4
52-53	8.782732585641743E-6	0.0011066243057908595	0.0	0.16738570898345317	3.820488674754158E-4
54-55	8.782732585641743E-6	0.0011154070383765013	0.0	0.2290624485661223	4.2157116411080365E-4
56-57	8.782732585641743E-6	0.0011373638698406055	0.0	0.27310785248311564	4.3474526298926627E-4
58-59	8.782732585641743E-6	0.0011505379687190682	0.0	0.32191788882781963	4.479193618677289E-4
60-61	8.782732585641743E-6	0.001190060265354456	0.0	0.3421489133388454	4.5670209445337065E-4
62-63	8.782732585641743E-6	0.001194451631647277	0.0	0.35462039361045666	4.6548482703901237E-4
64-65	8.782732585641743E-6	0.0013349753530175448	0.0	0.3651860209109837	4.6548482703901237E-4
66-67	8.782732585641743E-6	0.001370106283360112	0.0	0.3719706818333919	4.6548482703901237E-4
68-69	8.782732585641743E-6	0.0014140199462883206	0.0	0.3785796881040873	4.6548482703901237E-4
70-71	8.782732585641743E-6	0.0014403681440452458	0.0	0.3845782944600806	4.8305029221029584E-4
72-73	8.782732585641743E-6	0.0014535422429237084	0.0	0.38924192546305636	6.235740135805638E-4
74-75	8.782732585641743E-6	0.0014754990743878127	0.0	0.39306241413781057	6.235740135805638E-4
76-77	8.782732585641743E-6	0.0015194127373160215	0.0	0.39659307263723853	6.455308450446681E-4
78-79	8.782732585641743E-6	0.0015457609350729468	0.0	0.3998251182287547	6.587049439231307E-4
80-81	8.782732585641743E-6	0.0015545436676585884	0.0	0.4011952245121148	6.80661775387235E-4
82-83	8.782732585641743E-6	0.001567717766537051	0.0	0.401752928031303	7.114013394369811E-4
84-85	8.782732585641743E-6	0.001572109132829872	0.0	0.40208667186955743	7.201840720226229E-4
86-87	8.782732585641743E-6	0.0015940659642939762	0.0	0.40227989198644154	7.245754383154437E-4
88-89	8.782732585641743E-6	0.0016599364586862893	0.0	0.40236332794600516	7.333581709010855E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17975	0.0	51.59545	1
GTATCAA	33190	0.0	37.454975	1
ATCAACG	41265	0.0	29.65476	3
TCAACGC	43200	0.0	28.404251	4
TATCAAC	43995	0.0	27.922224	2
CAACGCA	44060	0.0	27.849833	5
AACGCAG	44400	0.0	27.674648	6
ACGCAGA	45045	0.0	27.287668	7
CGCAGAG	51845	0.0	23.708612	8
GCAGAGT	59035	0.0	20.736446	9
GTACATG	38740	0.0	19.832754	1
GAGTACT	35355	0.0	19.627647	12-13
GTGGTAT	8750	0.0	19.523544	1
TGGTATC	8385	0.0	19.42405	2
TACATGG	38870	0.0	19.205696	2
AGAGTAC	54310	0.0	19.1797	10-11
ACATGGG	38265	0.0	19.157192	3
CAGAGTA	57665	0.0	18.419569	10-11
AGTACTT	37855	0.0	18.137865	12-13
CTTATAC	11410	0.0	18.017826	1
>>END_MODULE