FastQCFastQC Report
Fri 27 May 2016
SRR2139075_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139075_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11525746
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150500.048.514021
GTATCAA273650.037.3725241
TCAACGC342700.029.219854
ATCAACG342800.029.1864683
CAACGCA351050.028.5127585
TATCAAC355100.028.4540422
AACGCAG362050.027.6815976
ACGCAGA412850.024.2857237
CGCAGAG417800.023.9878448
GCAGAGT482550.020.619829
CTTATAC121250.020.5270711
TACATGG315600.019.4671342
GTACATG325600.019.3771171
GAGTACT302350.019.16122212-13
AGAGTAC442300.019.06999210-11
ACATGGG321700.018.4100783
CAGAGTA473400.018.11272610-11
AGTACTT330200.017.60288812-13
TATACAC174050.017.367043
GTGGTAT79150.017.3030321
Produced by FastQC (version 0.11.5)