FastQCFastQC Report
Fri 27 May 2016
SRR2139074_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139074_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10942335
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140750.049.4675251
GTATCAA256500.036.286121
ATCAACG314350.029.0526053
TCAACGC315900.028.9636424
CAACGCA325250.028.131025
TATCAAC329700.027.9346122
AACGCAG334650.027.4804556
CTTATAC124750.024.5301631
ACGCAGA383300.023.9041987
CGCAGAG383900.023.7896698
TATACAC168800.020.7338623
GCAGAGT446500.020.4166879
GTACATG305750.019.9618821
TACATGG302700.019.4918482
GAGTACT268100.019.25102812-13
AGAGTAC405350.018.78856510-11
TTATACA174750.018.746772
ACATGGG307250.018.622243
CAGAGTA439200.018.05839710-11
CATGGGG219250.017.1403834
Produced by FastQC (version 0.11.5)