##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139074_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10942335
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.588749476231534	34.0	31.0	34.0	31.0	34.0
2	32.72145817140491	34.0	31.0	34.0	31.0	34.0
3	32.70882905705226	34.0	31.0	34.0	31.0	34.0
4	36.224063693900796	37.0	35.0	37.0	35.0	37.0
5	36.18045974648007	37.0	35.0	37.0	35.0	37.0
6	36.22276086411173	37.0	37.0	37.0	35.0	37.0
7	36.18251049707398	37.0	37.0	37.0	35.0	37.0
8	36.159207335545844	37.0	37.0	37.0	35.0	37.0
9	37.8955748475988	39.0	38.0	39.0	35.0	39.0
10-11	37.82674424608642	39.0	38.0	39.0	35.0	39.0
12-13	37.79812832453037	39.0	38.0	39.0	35.0	39.0
14-15	39.15295172374087	40.0	39.0	41.0	36.0	41.0
16-17	39.12110472764725	40.0	38.5	41.0	36.0	41.0
18-19	39.083882873262425	40.0	38.5	41.0	36.0	41.0
20-21	39.069823236817655	40.0	39.0	41.0	36.0	41.0
22-23	38.954351965060866	40.0	38.0	41.0	35.0	41.0
24-25	38.815186784790754	40.0	38.0	41.0	35.0	41.0
26-27	38.767381335465046	40.0	38.0	41.0	34.5	41.0
28-29	38.712913416372274	40.0	38.0	41.0	35.0	41.0
30-31	38.65074488273751	40.0	38.0	41.0	35.0	41.0
32-33	38.54087099452103	40.0	38.0	41.0	34.5	41.0
34-35	38.348455643867595	40.0	38.0	41.0	34.0	41.0
36-37	38.4826702108245	40.0	38.0	41.0	34.0	41.0
38-39	38.46140582119346	40.0	38.0	41.0	34.0	41.0
40-41	38.43438734294041	40.0	38.0	41.0	34.0	41.0
42-43	38.47186502814952	40.0	38.0	41.0	34.0	41.0
44-45	38.4142795476407	40.0	38.0	41.0	34.0	41.0
46-47	38.358220078549735	40.0	38.0	41.0	34.0	41.0
48-49	38.20652299414445	40.0	38.0	41.0	33.0	41.0
50-51	38.204608242357	40.0	38.0	41.0	33.0	41.0
52-53	38.12166773320362	40.0	37.0	41.0	33.0	41.0
54-55	37.97726829384807	40.0	37.0	41.0	33.0	41.0
56-57	37.80829312897325	40.0	37.0	41.0	33.0	41.0
58-59	37.64999748178202	39.5	36.0	41.0	33.0	41.0
60-61	37.46278846997662	39.0	36.0	41.0	32.0	41.0
62-63	37.258728787845584	39.0	35.5	40.5	32.0	41.0
64-65	36.97699236470059	38.5	35.0	40.0	31.5	41.0
66-67	36.6296287346442	38.0	35.0	40.0	31.0	41.0
68-69	36.30307398073133	37.5	35.0	40.0	31.0	41.0
70-71	36.00351425745319	37.0	35.0	39.5	31.0	41.0
72-73	35.68032157459361	36.0	34.0	39.0	30.5	41.0
74-75	35.31153467217794	36.0	34.0	39.0	30.0	40.0
76-77	34.34215857604477	35.0	33.0	37.0	29.0	39.0
78-79	34.62158731870356	35.0	34.0	37.0	30.0	39.0
80-81	34.419885077942425	35.0	34.0	37.0	30.0	39.0
82-83	34.13796282419274	35.0	34.0	36.0	29.0	38.5
84-85	33.88533532903334	35.0	33.5	36.0	29.0	37.0
86-87	33.64010046601141	35.0	33.0	35.5	29.0	37.0
88-89	33.397641661989915	35.0	33.0	35.0	29.0	36.5
90-91	33.21043145175273	35.0	33.0	35.0	29.0	36.0
92-93	33.11651758725039	35.0	33.0	35.0	29.0	36.0
94-95	33.036007054009175	34.5	33.0	35.0	29.0	36.0
96-97	33.01474532901855	34.0	32.5	35.0	29.0	35.0
98-99	33.13070156926155	34.0	33.0	35.0	29.0	35.0
100-101	31.255681108951144	32.5	29.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	0.0
19	9.0
20	44.0
21	306.0
22	1494.0
23	5194.0
24	12969.0
25	26725.0
26	47150.0
27	74258.0
28	106680.0
29	143104.0
30	183571.0
31	232449.0
32	292945.0
33	382196.0
34	527577.0
35	762717.0
36	1268774.0
37	2367650.0
38	2963282.0
39	1459061.0
40	84177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.273236612338515	21.663195590921973	15.605200463191899	24.45836733354761
2	13.408368575321822	21.737274951857987	41.92278119889424	22.93157527392595
3	16.67285212394834	26.86090799832175	31.609145874101984	24.857094003627928
4	11.702372074660584	19.111568661855987	39.20567119221518	29.980388071268248
5	12.686937968347333	38.14033990740366	36.083356264153174	13.08936586009583
6	29.080221824703944	38.90665373584831	18.890375673051103	13.122748766396638
7	25.670174724165314	33.25405103280991	23.3787620976258	17.697012145398975
8	23.913371681032583	37.347324620140455	21.35528855178429	17.38401514704267
9	25.012762359474678	17.027820572832088	21.649713854466516	36.30970321322672
10-11	23.508779240889396	27.241292601746146	29.333924981255116	19.91600317610934
12-13	24.408223093279567	24.73622672178144	29.43795324858482	21.417596936354172
14-15	21.576180798585455	26.277030656631723	27.037382823973537	25.109405720809285
16-17	21.110435688997804	29.511950074430217	27.703956318919122	21.673657917652857
18-19	21.078549505201586	28.484870916490856	29.571151861097288	20.865427717210267
20-21	21.782904458241312	27.526777171282824	29.564824728604638	21.12549364187122
22-23	21.487150656825857	27.711655057844254	29.389465633449785	21.411728651880104
24-25	21.351524635135092	27.85661900294364	29.528650984928174	21.263205376993092
26-27	21.27306975804698	28.00479910142149	29.427458475110036	21.294672665421498
28-29	21.05394797643048	27.868643495782347	29.488078385258966	21.58933014252821
30-31	21.32675720693371	27.71457708835321	29.541665625326186	21.4170000793869
32-33	21.14906152849705	27.895105259561927	29.502833581918996	21.452999630022028
34-35	21.282149063004788	28.13364202595863	29.506708004025995	21.07750090701059
36-37	21.371954751174762	27.990193139472186	29.190921515878575	21.446930593474477
38-39	21.36565598473438	27.9158901943705	29.31682535782868	21.40162846306644
40-41	21.47529773373356	27.841554700576253	29.284801715090907	21.398345850599274
42-43	21.521913687956694	27.906788933145815	29.138864571637583	21.43243280725991
44-45	21.521796952973247	28.102560602200754	28.85042311777041	21.525219327055588
46-47	21.703946872046693	27.979554838008227	28.43823031015654	21.878267979788543
48-49	21.671870464239475	28.10340589514237	28.397899821174793	21.82682381944336
50-51	21.521332944408485	28.244472087172067	28.39994895973624	21.834246008683213
52-53	21.6029093537441	28.287901012457866	28.442690006798433	21.6664996269996
54-55	21.603290879402476	28.265150752378954	28.281830598428904	21.84972776978967
56-57	21.814582065114585	28.38826517913861	28.043891874561695	21.753260881185113
58-59	21.53064576714531	28.572446076311923	28.339530090047372	21.557378066495392
60-61	21.81885431374165	28.65774215488847	27.984830877294637	21.538572654075246
62-63	21.57919730153159	28.85361794573244	27.928091057397115	21.639093695338858
64-65	21.568408969095014	28.695814719948785	28.007021807128478	21.728754503827716
66-67	21.64744439441122	28.588011263818153	27.94636156264336	21.818182779127266
68-69	21.663043305553906	28.464392686354778	28.01347561853441	21.85908838955691
70-71	21.6604687291882	28.251271660095433	28.109793354355357	21.978466256361006
72-73	21.725338511606076	28.242906157734055	28.101485147567264	21.930270183092603
74-75	21.66184078224381	28.303070001038716	28.046751495873075	21.9883377208444
76-77	21.73631827337139	28.241167910366844	28.00538207645352	22.01713173980825
78-79	21.66562564875166	28.24177953909064	28.001405034968702	22.091189777188998
80-81	21.68031499217384	28.28199353876882	27.998838237440232	22.03885323161711
82-83	21.72136063320776	28.228482644418158	28.074750204341132	21.97540651803295
84-85	21.85722289993876	28.18423034861337	28.046755086179804	21.91179166526807
86-87	21.69666036358809	28.166107462483815	28.142745251324282	21.994486922603816
88-89	21.746293394597892	28.09465833029334	28.146599234615444	22.012449040493326
90-91	21.76123427918319	28.14072656524488	28.151285288485532	21.9467538670864
92-93	21.749025563729933	28.04691800004719	28.23568830472994	21.968368131492937
94-95	21.597822809364313	28.058413767476576	28.310024864984314	22.033738558174797
96-97	21.51723216344817	28.17606869548707	28.361106406774816	21.945592734289935
98-99	21.69979003638171	28.66108364609693	28.418282766283365	21.220843551237998
100-101	20.26540712031664	32.18743654076164	26.28362612787169	21.263530211050025
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1949.0
1	1790.0
2	2592.0
3	3802.0
4	5359.0
5	8399.5
6	10822.0
7	12954.5
8	14570.5
9	14593.0
10	13455.5
11	12558.0
12	14250.5
13	19905.0
14	33279.5
15	51224.0
16	65326.5
17	72585.5
18	73435.0
19	69732.5
20	63978.0
21	58620.0
22	56517.5
23	60522.0
24	70569.0
25	86656.5
26	107574.5
27	130600.5
28	155762.0
29	182990.0
30	209961.5
31	237194.5
32	264631.5
33	289701.5
34	315198.5
35	340729.0
36	365504.0
37	387505.5
38	403697.5
39	416238.5
40	427588.0
41	437515.0
42	443607.5
43	448712.5
44	454890.0
45	463996.5
46	475167.0
47	478496.5
48	465798.5
49	449283.5
50	434174.5
51	410861.0
52	382046.0
53	353226.5
54	327832.5
55	300607.5
56	270950.5
57	241170.0
58	209552.0
59	177176.0
60	145660.5
61	117002.0
62	91187.5
63	69137.0
64	50894.0
65	37455.5
66	28016.0
67	21341.5
68	16266.0
69	11699.5
70	8510.5
71	6187.0
72	4319.0
73	2946.5
74	2000.5
75	1336.0
76	856.5
77	528.5
78	357.5
79	255.0
80	164.5
81	108.0
82	75.5
83	53.0
84	40.0
85	28.5
86	22.5
87	17.5
88	11.0
89	7.5
90	4.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.8277634526817175E-5
2	0.016331066449711145
3	1.8277634526817175E-5
4	1.370822589511288E-4
5	1.8277634526817175E-5
6	9.138817263408588E-6
7	1.8277634526817175E-5
8	9.138817263408588E-6
9	9.138817263408588E-6
10-11	1.507904848462417E-4
12-13	7.128277465458698E-4
14-15	3.381362387461177E-4
16-17	0.0018871657648938732
18-19	0.0
20-21	0.0
22-23	2.3306551668180148E-4
24-25	0.00941111942728836
26-27	0.019938927718209414
28-29	0.34798721618003103
30-31	0.32388714024401555
32-33	0.31759458973976995
34-35	0.18773225703671406
36-37	0.0478622506762144
38-39	0.0390933232743414
40-41	0.01746834558801841
42-43	0.016101984137989193
44-45	0.019012218407986788
46-47	0.013568382073330517
48-49	0.008414652721746857
50-51	0.005270290729514524
52-53	0.002350020088722145
54-55	3.432464739011051E-4
56-57	6.22342403455245E-4
58-59	0.0019503002702558948
60-61	0.0021534088462035403
62-63	7.387546177975493E-4
64-65	3.602788119404126E-4
66-67	0.003250292328120974
68-69	0.00528001859080102
70-71	0.006762109423264557
72-73	0.010963998494241085
74-75	0.00821398866591171
76-77	0.006343485723608123
78-79	0.0038047342540111816
80-81	0.004761282895629131
82-83	0.0017556862360399042
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.002385159915161885
92-93	0.015470991205000494
94-95	0.001273641573698792
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	191.0
20-21	714.0
22-23	1478.0
24-25	3110.0
26-27	4570.0
28-29	8260.0
30-31	22577.0
32-33	29999.0
34-35	44209.0
36-37	59594.0
38-39	70058.0
40-41	75477.0
42-43	89944.0
44-45	106253.0
46-47	104627.0
48-49	91880.0
50-51	82732.0
52-53	74625.0
54-55	85526.0
56-57	92501.0
58-59	94482.0
60-61	97494.0
62-63	99855.0
64-65	110038.0
66-67	118534.0
68-69	105803.0
70-71	99799.0
72-73	97978.0
74-75	99133.0
76-77	100416.0
78-79	106246.0
80-81	108170.0
82-83	108178.0
84-85	112041.0
86-87	118932.0
88-89	128351.0
90-91	140431.0
92-93	152528.0
94-95	183375.0
96-97	278750.0
98-99	1035880.0
100-101	6397596.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.92907977449858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.64059177224246	73.50882706339057
2	7.4811020263885055	12.408018134950707
3	2.0862567602003135	5.1903405989021625
4	0.8133852013047959	2.69813144985608
5	0.3692009509111681	1.5308747555466498
6	0.1994674351151653	0.9924990503448096
7	0.11637372666847529	0.6755536243781975
8	0.08523270180780806	0.5654615622092611
9	0.0525567914211995	0.3922637713614302
>10	0.1537675313171643	1.8691559511699862
>50	0.0016914300487497912	0.09097574284565291
>100	3.3573509034715677E-4	0.05165967236907136
>500	3.793748378573324E-5	0.026238622675395326
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8277634526817175E-5
2	0.0	0.0	0.0	0.0	1.8277634526817175E-5
3	0.0	0.0	0.0	0.0	1.8277634526817175E-5
4	9.138817263408588E-6	9.138817263408588E-6	0.0	1.8277634526817175E-5	1.8277634526817175E-5
5	1.8277634526817175E-5	1.8277634526817175E-5	0.0	1.8277634526817175E-5	1.8277634526817175E-5
6	1.8277634526817175E-5	1.8277634526817175E-5	0.0	4.5694086317042936E-5	2.741645179022576E-5
7	2.741645179022576E-5	1.8277634526817175E-5	0.0	4.5694086317042936E-5	2.741645179022576E-5
8	2.741645179022576E-5	1.8277634526817175E-5	0.0	4.5694086317042936E-5	2.741645179022576E-5
9	3.655526905363435E-5	2.741645179022576E-5	0.0	4.5694086317042936E-5	2.741645179022576E-5
10-11	5.026349494874723E-5	2.741645179022576E-5	0.0	5.026349494874723E-5	2.741645179022576E-5
12-13	5.483290358045152E-5	2.741645179022576E-5	0.0	7.31105381072687E-5	2.741645179022576E-5
14-15	5.483290358045152E-5	3.655526905363435E-5	0.0	7.31105381072687E-5	8.681876400238158E-5
16-17	5.483290358045152E-5	3.655526905363435E-5	0.0	9.138817263408587E-5	1.2794344168772022E-4
18-19	5.940231221215581E-5	3.655526905363435E-5	0.0	1.0509639852919875E-4	1.507904848462417E-4
20-21	8.224935537067728E-5	3.655526905363435E-5	0.0	1.416516675828331E-4	1.6449871074135456E-4
22-23	1.0052698989749445E-4	5.483290358045152E-5	0.0	3.518444646412306E-4	1.8734575389987602E-4
24-25	1.0052698989749445E-4	7.311053810726869E-5	0.0	0.0010646722111871004	2.010539797949889E-4
26-27	1.0052698989749445E-4	8.224935537067728E-5	0.0	0.003303682440722204	2.010539797949889E-4
28-29	1.0509639852919875E-4	8.224935537067728E-5	0.0	0.018382730925346372	2.2390102295351039E-4
30-31	1.0966580716090304E-4	8.224935537067728E-5	0.0	0.025913116350395046	2.3760924884862325E-4
32-33	1.0966580716090304E-4	8.224935537067728E-5	0.0	0.028398874646042183	2.5588688337544043E-4
34-35	1.1880462442431162E-4	8.681876400238158E-5	0.0	0.03163858536592053	2.65025700638849E-4
36-37	1.1880462442431162E-4	9.138817263408587E-5	0.0	0.03710359808943886	2.65025700638849E-4
38-39	1.507904848462417E-4	9.138817263408587E-5	0.0	0.04667650917285936	2.65025700638849E-4
40-41	1.6906811937305886E-4	1.0052698989749445E-4	0.0	0.06427330181355259	2.741645179022576E-4
42-43	1.7363752800476315E-4	1.0052698989749445E-4	0.0	0.07335728617338072	2.9244215242907475E-4
44-45	1.7363752800476315E-4	1.0509639852919875E-4	0.0	0.08581806351203833	3.152891955875962E-4
46-47	1.7363752800476315E-4	1.0966580716090304E-4	0.0	0.10127180350446226	3.335668301144134E-4
48-49	1.7363752800476315E-4	1.0966580716090304E-4	0.0	0.11504857052905071	3.655526905363435E-4
50-51	1.8277634526817175E-4	1.370822589511288E-4	0.0	0.1311374583212815	3.655526905363435E-4
52-53	1.8277634526817175E-4	1.416516675828331E-4	0.0	0.14806254789311424	3.838303250631607E-4
54-55	1.8277634526817175E-4	1.507904848462417E-4	0.0	0.21088734717041657	4.1581618548509067E-4
56-57	1.8734575389987604E-4	1.6449871074135456E-4	0.0	0.25029849661886605	4.386632286436122E-4
58-59	1.9191516253158034E-4	1.6449871074135456E-4	0.0	0.29909978080546795	4.4780204590702077E-4
60-61	1.9191516253158034E-4	1.6906811937305886E-4	0.0	0.3205805707831098	4.5694086317042936E-4
62-63	1.9191516253158034E-4	1.7363752800476315E-4	0.0	0.3319309818242633	4.6607968043383796E-4
64-65	1.9191516253158034E-4	1.9191516253158034E-4	0.0	0.3427924661418244	4.6607968043383796E-4
66-67	1.9191516253158034E-4	1.964845711632846E-4	0.0	0.3497333978533832	4.706490890655422E-4
68-69	1.9191516253158034E-4	2.010539797949889E-4	0.0	0.35594322418386937	4.752184976972465E-4
70-71	1.9191516253158034E-4	2.010539797949889E-4	0.0	0.3616458461562363	4.843573149606551E-4
72-73	2.101927970583975E-4	2.010539797949889E-4	0.0	0.36641173935910387	5.574678530679238E-4
74-75	2.193316143218061E-4	2.056233884266932E-4	0.0	0.3702409037924721	5.574678530679238E-4
76-77	2.193316143218061E-4	2.2390102295351039E-4	0.0	0.37350803096414065	5.574678530679238E-4
78-79	2.193316143218061E-4	2.2847043158521468E-4	0.0	0.3767386028667556	5.574678530679238E-4
80-81	2.193316143218061E-4	2.2847043158521468E-4	0.0	0.37819624422026926	5.75745487594741E-4
82-83	2.193316143218061E-4	2.2847043158521468E-4	0.0	0.3786806015352299	5.940231221215581E-4
84-85	2.193316143218061E-4	2.2847043158521468E-4	0.0	0.37905986245166134	5.940231221215581E-4
86-87	2.193316143218061E-4	2.2847043158521468E-4	0.0	0.3792563470228246	6.031619393849667E-4
88-89	2.2390102295351039E-4	2.65025700638849E-4	0.0	0.3793066105177734	6.168701652800796E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14075	0.0	49.467525	1
GTATCAA	25650	0.0	36.28612	1
ATCAACG	31435	0.0	29.052605	3
TCAACGC	31590	0.0	28.963642	4
CAACGCA	32525	0.0	28.13102	5
TATCAAC	32970	0.0	27.934612	2
AACGCAG	33465	0.0	27.480455	6
CTTATAC	12475	0.0	24.530163	1
ACGCAGA	38330	0.0	23.904198	7
CGCAGAG	38390	0.0	23.789669	8
TATACAC	16880	0.0	20.733862	3
GCAGAGT	44650	0.0	20.416687	9
GTACATG	30575	0.0	19.961882	1
TACATGG	30270	0.0	19.491848	2
GAGTACT	26810	0.0	19.251028	12-13
AGAGTAC	40535	0.0	18.788565	10-11
TTATACA	17475	0.0	18.74677	2
ACATGGG	30725	0.0	18.62224	3
CAGAGTA	43920	0.0	18.058397	10-11
CATGGGG	21925	0.0	17.140383	4
>>END_MODULE