FastQCFastQC Report
Fri 27 May 2016
SRR2139073_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139073_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8680163
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100450.052.1512831
GTATCAA188950.038.6350941
TCAACGC240150.029.8647564
ATCAACG242750.029.4219073
CAACGCA247650.028.9601465
TATCAAC252950.028.5077042
AACGCAG255800.028.1042586
ACGCAGA295500.024.271087
CGCAGAG299300.023.892078
GTACATG250600.021.3950841
TACATGG254800.020.443112
GCAGAGT351900.020.1641379
ACATGGG258300.019.473693
GAGTACT207650.018.75080712-13
AGAGTAC317900.018.7391210-11
GTGGTAT49400.018.289451
CAGAGTA341500.017.89124510-11
TGGTATC47800.017.3930662
CATGGGG198800.017.3662474
AGTACTT226950.017.19360412-13
Produced by FastQC (version 0.11.5)