##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139073_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8680163
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55379720403868	34.0	31.0	34.0	31.0	34.0
2	32.68383485425331	34.0	31.0	34.0	31.0	34.0
3	32.671730242853734	34.0	31.0	34.0	31.0	34.0
4	36.2014835435694	37.0	35.0	37.0	35.0	37.0
5	36.15415563048759	37.0	35.0	37.0	35.0	37.0
6	36.19759306363256	37.0	37.0	37.0	35.0	37.0
7	36.15615778182967	37.0	37.0	37.0	35.0	37.0
8	36.13199475631967	37.0	36.0	37.0	35.0	37.0
9	37.862277240646286	39.0	38.0	39.0	35.0	39.0
10-11	37.78994253909748	39.0	38.0	39.0	35.0	39.0
12-13	37.75966614912646	39.0	38.0	39.0	35.0	39.0
14-15	39.10216530496029	40.0	38.5	41.0	36.0	41.0
16-17	39.07125684160539	40.0	38.0	41.0	36.0	41.0
18-19	39.02919415222963	40.0	38.5	41.0	36.0	41.0
20-21	39.010190979252066	40.0	38.5	41.0	35.0	41.0
22-23	38.89229672871497	40.0	38.0	41.0	35.0	41.0
24-25	38.75482463324964	40.0	38.0	41.0	34.5	41.0
26-27	38.70383070235458	40.0	38.0	41.0	34.5	41.0
28-29	38.65012061653832	40.0	38.0	41.0	35.0	41.0
30-31	38.58745944123032	40.0	38.0	41.0	34.5	41.0
32-33	38.48121076904583	40.0	38.0	41.0	34.0	41.0
34-35	38.28618144142442	40.0	38.0	41.0	34.0	41.0
36-37	38.425810190614015	40.0	38.0	41.0	34.0	41.0
38-39	38.41260954438157	40.0	38.0	41.0	34.0	41.0
40-41	38.37493052722907	40.0	38.0	41.0	34.0	41.0
42-43	38.405981794073696	40.0	38.0	41.0	34.0	41.0
44-45	38.33752062349883	40.0	38.0	41.0	34.0	41.0
46-47	38.27530343664733	40.0	38.0	41.0	33.0	41.0
48-49	38.112859720563335	40.0	38.0	41.0	33.0	41.0
50-51	38.109826752738414	40.0	37.0	41.0	33.0	41.0
52-53	38.0092952618598	40.0	37.0	41.0	33.0	41.0
54-55	37.851751461604614	40.0	37.0	41.0	33.0	41.0
56-57	37.67489855519856	39.5	36.0	41.0	33.0	41.0
58-59	37.49899624493693	39.0	36.0	41.0	32.0	41.0
60-61	37.30886885726548	39.0	35.5	41.0	32.0	41.0
62-63	37.0839806236464	39.0	35.0	40.0	32.0	41.0
64-65	36.77744519559732	38.0	35.0	40.0	31.0	41.0
66-67	36.40818536012168	37.5	35.0	40.0	31.0	41.0
68-69	36.063713760007204	37.0	35.0	40.0	31.0	41.0
70-71	35.75468045204199	36.5	34.0	39.0	30.5	41.0
72-73	35.426449395368984	36.0	34.0	39.0	30.0	40.5
74-75	35.06058123570952	35.5	34.0	38.5	30.0	40.0
76-77	34.11566493321196	35.0	32.5	37.0	29.0	39.0
78-79	34.401785960167345	35.0	34.0	37.0	29.5	39.0
80-81	34.215906519821615	35.0	34.0	36.5	29.0	39.0
82-83	33.953728760628785	35.0	33.5	36.0	29.0	37.5
84-85	33.7239513239187	35.0	33.0	36.0	29.0	37.0
86-87	33.49924722966172	35.0	33.0	35.0	29.0	37.0
88-89	33.27519642661001	35.0	33.0	35.0	29.0	36.0
90-91	33.10110101209105	35.0	33.0	35.0	29.0	36.0
92-93	33.02519739059392	35.0	33.0	35.0	29.0	36.0
94-95	32.956543172821654	34.0	32.5	35.0	29.0	35.5
96-97	32.942297333499894	34.0	32.0	35.0	29.0	35.0
98-99	33.06989997613094	34.0	32.5	35.0	29.0	35.0
100-101	31.208469144541887	32.5	29.0	34.5	24.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	12.0
20	45.0
21	250.0
22	1230.0
23	4200.0
24	10596.0
25	21990.0
26	39563.0
27	61700.0
28	88612.0
29	118631.0
30	152067.0
31	191537.0
32	242173.0
33	317706.0
34	440313.0
35	638371.0
36	1068319.0
37	1942274.0
38	2237331.0
39	1045623.0
40	57619.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.19807026643051	20.99156674725656	15.250095562732586	24.560267423580342
2	13.918262367260517	21.508134380139655	41.06988006003566	23.50372319256417
3	17.24925179967839	26.23581218875869	30.87859420135246	25.636341810210457
4	12.260198639355782	18.4117261527533	38.406062658112134	30.92201254977879
5	13.215145062960806	37.80065395092856	35.49904944170397	13.485151544406659
6	30.34657997703038	37.90895122797838	18.394831616602502	13.349637178388743
7	26.607916604311832	32.677585127741295	22.926429590418888	17.788068677527985
8	24.53447297412191	36.919391596608456	20.79176632878511	17.754369100484528
9	25.567679497225974	16.795285617941232	21.162058956964167	36.47497592786863
10-11	24.240971781001356	26.807403562958914	28.751887209257447	20.199737446782283
12-13	25.025604502429346	24.376379158153913	28.94220628899574	21.655810050420996
14-15	22.149531407489484	25.76816994835223	26.559216823962462	25.52308182019582
16-17	21.730752858034727	28.898503836922636	27.23572791391892	22.135015391123712
18-19	21.787931862569863	28.051074616916754	28.996097193105708	21.164896327407675
20-21	22.52053449893749	27.10607431763366	28.973894299534386	21.399496883894468
22-23	22.253491121383313	27.03664181778138	28.825845667377813	21.88402139345749
24-25	22.05637697349776	27.22595656878687	28.829689478547227	21.887976979168144
26-27	22.003637398442606	27.28656487116673	28.82680928460193	21.882988445788744
28-29	21.908242145315754	27.35888777488198	28.829292026682314	21.90357805311996
30-31	22.14677827228142	27.220618473585755	28.75913409852241	21.873469155610415
32-33	21.980152502936377	27.349146281329695	28.715113567467586	21.955587648266338
34-35	22.142348808664263	27.401160939103875	28.650344765548784	21.806145486683075
36-37	22.098461893398895	27.342741676576495	28.595465092944757	21.963331337079854
38-39	22.06577909183437	27.434099181941107	28.607047215109304	21.893074511115216
40-41	22.24388347698642	27.288989136808578	28.487337376260246	21.97979000994476
42-43	22.124971543204015	27.432690310844553	28.307113830276414	22.135224315675014
44-45	22.2198255819186	27.496683536481097	28.12110266787065	22.16238821372965
46-47	22.301919415716792	27.50975247821193	27.87609922205637	22.312228884014907
48-49	22.27903544385326	27.650785797684684	27.748823081351176	22.32135567711088
50-51	22.26227633083485	27.76543016746257	27.657246416089126	22.31504708561345
52-53	22.42895178869042	27.65940791067441	27.566178922619628	22.345461378015543
54-55	22.335027640633452	27.570266013624394	27.611431780486196	22.483274565255954
56-57	22.357419565527813	27.68340144845985	27.617575587174397	22.34160339883794
58-59	22.31954391420484	27.718374995016543	27.627412988876927	22.334668101901688
60-61	22.429003680792356	27.697927074454327	27.576641548293875	22.296427696459446
62-63	22.351559221723228	27.773621718446474	27.598081899395417	22.276737160434884
64-65	22.311543603595663	27.736276216621224	27.548964977343466	22.403215202439643
66-67	22.34795183738854	27.769467498802403	27.42669611342793	22.45588455038113
68-69	22.287852500159506	27.75342454002137	27.489918992719588	22.46880396709954
70-71	22.2868710790477	27.573020135064922	27.51845508753852	22.621653698348858
72-73	22.370702847465076	27.559289422914368	27.423034652867152	22.646973076753408
74-75	22.256072100309574	27.621542067031207	27.46175633156603	22.66062950109319
76-77	22.41195863147507	27.573627211743297	27.411272759890537	22.603141396891093
78-79	22.35290292615614	27.553628180451668	27.384802625898253	22.70866626749394
80-81	22.37491571265306	27.594567660320934	27.3623171610867	22.668199465939306
82-83	22.388008216355807	27.558517207034523	27.44297697292575	22.610497603683925
84-85	22.447077486315198	27.55311374493752	27.430894909306367	22.56891385944091
86-87	22.348432493185644	27.549320487857322	27.489306743591612	22.612940275365425
88-89	22.33187193681368	27.50622340648487	27.519403364051765	22.642501292649683
90-91	22.387160858204464	27.48142921710903	27.50338213314063	22.628027791545875
92-93	22.390162291471754	27.390604992113193	27.64271341752448	22.57651929889057
94-95	22.23004229149211	27.39746132800862	27.692670052617284	22.679826327881987
96-97	22.1733457485515	27.472900048339998	27.735175494202107	22.61857870890639
98-99	22.366308180320132	28.033240896927975	27.73803408922208	21.86241683352981
100-101	21.04618682261293	31.548779747832256	25.455269903966453	21.949763525588363
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1787.0
1	1612.0
2	2137.0
3	3031.5
4	4045.5
5	6062.5
6	7753.0
7	9715.5
8	11644.0
9	12162.5
10	11328.0
11	10319.0
12	11291.0
13	15058.5
14	24364.5
15	37053.5
16	46775.5
17	51565.0
18	51833.0
19	48918.0
20	44444.5
21	40008.5
22	38025.0
23	40602.5
24	47498.0
25	58573.5
26	72990.0
27	88982.5
28	106978.0
29	126367.0
30	145226.5
31	163800.5
32	183712.0
33	203447.5
34	221359.0
35	239063.5
36	258682.0
37	276096.0
38	290774.5
39	304575.0
40	316124.0
41	327698.5
42	335824.5
43	341414.5
44	348373.5
45	354524.5
46	359187.5
47	360968.5
48	358676.0
49	354643.5
50	350951.0
51	343183.5
52	330697.0
53	316033.5
54	299976.0
55	282001.5
56	261098.5
57	237892.0
58	211847.0
59	183026.5
60	153050.0
61	125269.0
62	100272.5
63	77506.0
64	57797.5
65	43237.0
66	32812.5
67	24785.0
68	18708.5
69	13284.0
70	9182.0
71	6549.0
72	4490.0
73	2977.5
74	1978.0
75	1300.5
76	835.5
77	531.0
78	337.5
79	227.0
80	151.0
81	100.5
82	71.0
83	45.5
84	32.5
85	28.5
86	22.0
87	16.5
88	14.5
89	9.5
90	5.0
91	3.0
92	1.5
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.1520520985608219E-5
2	0.015218608221988459
3	1.1520520985608219E-5
4	1.152052098560822E-4
5	1.1520520985608219E-5
6	2.3041041971216438E-5
7	2.3041041971216438E-5
8	1.1520520985608219E-5
9	1.1520520985608219E-5
10-11	2.0736937774094795E-4
12-13	5.645055282948027E-4
14-15	2.304104197121644E-4
16-17	0.0018432833576973152
18-19	0.0
20-21	0.0
22-23	2.361964490801934E-4
24-25	0.008844784178911026
26-27	0.019085030989398327
28-29	0.34593835136262135
30-31	0.32239437808494137
32-33	0.3163712838076713
34-35	0.18619582105560686
36-37	0.045188326372337474
38-39	0.0364986746360131
40-41	0.015655054221268488
42-43	0.014597959413391556
44-45	0.018044544830555845
46-47	0.012398916409850726
48-49	0.007478157293920467
50-51	0.0040372812676322395
52-53	0.0015307689220306585
54-55	6.897534244376377E-5
56-57	2.2133730863571543E-4
58-59	0.001237476214463303
60-61	0.0011264583371016048
62-63	3.1183882454434013E-4
64-65	9.838109965277374E-5
66-67	0.0031621077403690785
68-69	0.005254018385847605
70-71	0.006673660120449741
72-73	0.01066442373584284
74-75	0.00793499123411963
76-77	0.005720520972116066
78-79	0.003470741402070446
80-81	0.004488908702552647
82-83	0.0016557345822607065
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0027219337109183374
92-93	0.015803206790792154
94-95	0.0013262604767586834
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	150.0
20-21	561.0
22-23	1123.0
24-25	3153.0
26-27	3762.0
28-29	6412.0
30-31	18414.0
32-33	23828.0
34-35	34147.0
36-37	48087.0
38-39	56112.0
40-41	59249.0
42-43	69986.0
44-45	83417.0
46-47	82415.0
48-49	73173.0
50-51	65686.0
52-53	60037.0
54-55	66467.0
56-57	67936.0
58-59	70186.0
60-61	72272.0
62-63	71170.0
64-65	79373.0
66-67	81830.0
68-69	81159.0
70-71	80597.0
72-73	78413.0
74-75	80255.0
76-77	80528.0
78-79	85601.0
80-81	87847.0
82-83	86615.0
84-85	89885.0
86-87	96290.0
88-89	103933.0
90-91	112606.0
92-93	123670.0
94-95	148555.0
96-97	225555.0
98-99	836575.0
100-101	5083133.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.5438810836915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.87129267491176	78.64354347178892
2	6.472802385819236	11.203628799131495
3	1.4794377439209923	3.841088525418696
4	0.5534871711328211	1.9160371167947072
5	0.22917642125880575	0.9916908474304037
6	0.12903775898869307	0.6700457081534098
7	0.07124107906435422	0.43158356323735353
8	0.04791072638142402	0.3317104165269794
9	0.03144490335902595	0.2449227579292542
>10	0.11100981792729618	1.508578197105074
>50	0.0026656679071995694	0.15299923291956125
>100	4.93649328375317E-4	0.06417136356418426
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1520520985608219E-5	0.0
2	0.0	3.456156295682466E-5	0.0	1.1520520985608219E-5	0.0
3	0.0	3.456156295682466E-5	0.0	1.1520520985608219E-5	0.0
4	0.0	4.6082083942432876E-5	0.0	1.1520520985608219E-5	0.0
5	0.0	4.6082083942432876E-5	0.0	2.3041041971216438E-5	0.0
6	0.0	4.6082083942432876E-5	0.0	3.456156295682466E-5	0.0
7	0.0	4.6082083942432876E-5	0.0	4.6082083942432876E-5	0.0
8	0.0	8.064364689925754E-5	0.0	4.6082083942432876E-5	0.0
9	0.0	1.0368468887047397E-4	0.0	5.76026049280411E-5	1.1520520985608219E-5
10-11	0.0	1.0368468887047397E-4	0.0	6.912312591364932E-5	1.7280781478412327E-5
12-13	1.7280781478412327E-5	1.0368468887047397E-4	0.0	8.640390739206164E-5	2.3041041971216438E-5
14-15	2.3041041971216438E-5	1.3824625182729864E-4	0.0	1.0944494936327809E-4	2.3041041971216438E-5
16-17	2.880130246402055E-5	1.4400651232010275E-4	0.0	1.4976677281290686E-4	2.880130246402055E-5
18-19	4.0321823449628765E-5	1.7280781478412328E-4	0.0	1.7280781478412328E-4	5.184234443523699E-5
20-21	4.6082083942432876E-5	2.0736937774094795E-4	0.0	2.4769120119057676E-4	6.912312591364932E-5
22-23	4.6082083942432876E-5	2.304104197121644E-4	0.0	4.493003184387205E-4	8.640390739206164E-5
24-25	4.6082083942432876E-5	2.4769120119057676E-4	0.0	0.0011808534010248426	1.0368468887047397E-4
26-27	5.184234443523699E-5	2.6497198266898906E-4	0.0	0.0034907178586392907	1.0368468887047397E-4
28-29	6.336286542084521E-5	2.6497198266898906E-4	0.0	0.019008859626253562	1.0368468887047397E-4
30-31	6.912312591364932E-5	2.880130246402055E-4	0.0	0.027119306400121748	1.0368468887047397E-4
32-33	6.912312591364932E-5	2.880130246402055E-4	0.0	0.029584697891041907	1.0368468887047397E-4
34-35	8.064364689925754E-5	3.340951085826384E-4	0.0	0.03272980012011295	1.0944494936327809E-4
36-37	9.216416788486575E-5	3.8017719252507123E-4	0.0	0.03775850753033094	1.3824625182729864E-4
38-39	9.216416788486575E-5	3.8017719252507123E-4	0.0	0.047470306721198674	1.3824625182729864E-4
40-41	1.0368468887047397E-4	4.262592764675041E-4	0.0	0.06528679242544178	1.612872937985151E-4
42-43	1.0368468887047397E-4	4.4930031843872057E-4	0.0	0.0738811010807055	1.612872937985151E-4
44-45	1.0368468887047397E-4	4.8386188139554523E-4	0.0	0.08674376276113709	1.612872937985151E-4
46-47	1.0368468887047397E-4	4.953824023811534E-4	0.0	0.10322386803104965	1.612872937985151E-4
48-49	1.0368468887047397E-4	4.953824023811534E-4	0.0	0.11726738311250606	1.843283357697315E-4
50-51	1.0368468887047397E-4	5.990670912516275E-4	0.0	0.1337186870799546	1.9008859626253562E-4
52-53	1.0368468887047397E-4	6.221081332228439E-4	0.0	0.1515006112212409	2.0736937774094795E-4
54-55	1.0368468887047397E-4	6.221081332228439E-4	0.0	0.21355013724972677	2.1888989872655617E-4
56-57	1.0368468887047397E-4	6.221081332228439E-4	0.0	0.2556461209311392	2.1888989872655617E-4
58-59	1.0368468887047397E-4	6.336286542084521E-4	0.0	0.3060656810246536	2.1888989872655617E-4
60-61	1.0368468887047397E-4	6.451491751940602E-4	0.0	0.328311807047863	2.1888989872655617E-4
62-63	1.0368468887047397E-4	6.566696961796685E-4	0.0	0.33933118537059725	2.1888989872655617E-4
64-65	1.0368468887047397E-4	7.142723011077096E-4	0.0	0.3501431943155906	2.1888989872655617E-4
66-67	1.0368468887047397E-4	7.142723011077096E-4	0.0	0.356836617008229	2.1888989872655617E-4
68-69	1.2096547034888631E-4	7.31553082586122E-4	0.0	0.36327658823918396	2.1888989872655617E-4
70-71	1.2672573084169042E-4	7.37313343078926E-4	0.0	0.36866819206044865	2.2465015921936028E-4
72-73	1.2672573084169042E-4	7.37313343078926E-4	0.0	0.373155434984343	2.5345146168338084E-4
74-75	1.2672573084169042E-4	7.661146455429466E-4	0.0	0.3769572069095938	2.5345146168338084E-4
76-77	1.2672573084169042E-4	8.237172504709878E-4	0.0	0.3803269592978842	2.5345146168338084E-4
78-79	1.2672573084169042E-4	8.525185529350082E-4	0.0	0.38354118465286885	2.5345146168338084E-4
80-81	1.2672573084169042E-4	8.525185529350082E-4	0.0	0.38474507909586486	2.5345146168338084E-4
82-83	1.3248599133449453E-4	8.582788134278124E-4	0.0	0.38526926280071006	2.6497198266898906E-4
84-85	1.3824625182729864E-4	8.813198553990287E-4	0.0	0.38567248103520635	2.6497198266898906E-4
86-87	1.3824625182729864E-4	8.986006368774411E-4	0.0	0.38575888494259847	2.6497198266898906E-4
88-89	1.3824625182729864E-4	9.677237627910905E-4	0.0	0.3858337683290049	2.764925036545973E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10045	0.0	52.151283	1
GTATCAA	18895	0.0	38.635094	1
TCAACGC	24015	0.0	29.864756	4
ATCAACG	24275	0.0	29.421907	3
CAACGCA	24765	0.0	28.960146	5
TATCAAC	25295	0.0	28.507704	2
AACGCAG	25580	0.0	28.104258	6
ACGCAGA	29550	0.0	24.27108	7
CGCAGAG	29930	0.0	23.89207	8
GTACATG	25060	0.0	21.395084	1
TACATGG	25480	0.0	20.44311	2
GCAGAGT	35190	0.0	20.164137	9
ACATGGG	25830	0.0	19.47369	3
GAGTACT	20765	0.0	18.750807	12-13
AGAGTAC	31790	0.0	18.73912	10-11
GTGGTAT	4940	0.0	18.28945	1
CAGAGTA	34150	0.0	17.891245	10-11
TGGTATC	4780	0.0	17.393066	2
CATGGGG	19880	0.0	17.366247	4
AGTACTT	22695	0.0	17.193604	12-13
>>END_MODULE