FastQCFastQC Report
Fri 27 May 2016
SRR2139072_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139072_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11071280
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC113900.048.8930781
GGTATCA144500.047.6630741
GTATCAA287700.041.0046581
TATACAC163700.036.6330833
ATCAACG346450.034.062793
TCAACGC351750.033.5617184
CAACGCA354350.033.2792245
TATCAAC367100.032.5205542
TTATACA177200.032.345262
AACGCAG368950.032.0541086
ACGCAGA411450.028.7117947
CGCAGAG418850.028.1636548
ACACATC209700.026.8205246
ACATCTC212350.026.6267958
TACACAT230050.026.179175
GCAGAGT483850.024.4244069
CACATCT228900.024.4211277
ATACACA269600.023.7200034
CATCTCC242300.022.9645659
TATGCCG146300.022.3566342-43
Produced by FastQC (version 0.11.5)