##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139072_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11071280
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.714661990302837	30.0	18.0	32.0	18.0	33.0
2	28.77707184715769	31.0	27.0	33.0	18.0	33.0
3	30.921588380024712	32.0	31.0	33.0	27.0	33.0
4	31.65766505769884	33.0	31.0	33.0	29.0	33.0
5	32.50787018303213	33.0	33.0	33.0	32.0	34.0
6	36.402347786344485	38.0	37.0	38.0	34.0	38.0
7	36.60050771003895	38.0	37.0	38.0	34.0	38.0
8	36.83399372068993	38.0	38.0	38.0	35.0	38.0
9	37.054046596238194	38.0	38.0	38.0	36.0	38.0
10-11	37.15184879255154	38.0	38.0	38.0	36.0	38.0
12-13	37.207290439768485	38.0	38.0	38.0	36.0	38.0
14-15	37.19984035269634	38.0	38.0	38.0	36.0	38.0
16-17	37.156328807509155	38.0	38.0	38.0	36.0	38.0
18-19	37.18633848118736	38.0	38.0	38.0	36.0	38.0
20-21	37.21116243928765	38.0	38.0	38.0	36.0	38.0
22-23	37.21954012733423	38.0	38.0	38.0	36.0	38.0
24-25	37.217689021099815	38.0	38.0	38.0	36.0	38.0
26-27	37.18895489240424	38.0	38.0	38.0	36.0	38.0
28-29	37.16522840783574	38.0	38.0	38.0	36.0	38.0
30-31	37.16678645196954	38.0	38.0	38.0	36.0	38.0
32-33	37.1511813076444	38.0	38.0	38.0	36.0	38.0
34-35	37.11997017894487	38.0	38.0	38.0	36.0	38.0
36-37	37.09917566189759	38.0	38.0	38.0	36.0	38.0
38-39	37.045272510337114	38.0	38.0	38.0	36.0	38.0
40-41	37.07329709265836	38.0	38.0	38.0	36.0	38.0
42-43	37.05784805259849	38.0	38.0	38.0	36.0	38.0
44-45	37.00829415038187	38.0	38.0	38.0	36.0	38.0
46-47	36.98191355333182	38.0	38.0	38.0	36.0	38.0
48-49	36.94931910795262	38.0	38.0	38.0	36.0	38.0
50-51	36.91150998769445	38.0	38.0	38.0	36.0	38.0
52-53	36.88693104581865	38.0	38.0	38.0	36.0	38.0
54-55	36.85597908945408	38.0	38.0	38.0	35.0	38.0
56-57	36.856424920827536	38.0	38.0	38.0	35.0	38.0
58-59	36.8356638848445	38.0	38.0	38.0	35.0	38.0
60-61	36.84133976002872	38.0	38.0	38.0	35.0	38.0
62-63	36.828304941536274	38.0	38.0	38.0	35.0	38.0
64-65	36.81615332936555	38.0	38.0	38.0	35.0	38.0
66-67	36.803293517750674	38.0	38.0	38.0	35.0	38.0
68-69	36.80723866440829	38.0	38.0	38.0	35.0	38.0
70-71	36.78402311306277	38.0	38.0	38.0	35.0	38.0
72-73	36.76558936462537	38.0	38.0	38.0	35.0	38.0
74-75	36.77949544248188	38.0	38.0	38.0	35.0	38.0
76-77	36.76099649204927	38.0	38.0	38.0	35.0	38.0
78-79	36.737893700570595	38.0	38.0	38.0	35.0	38.0
80-81	36.730211724233484	38.0	38.0	38.0	35.0	38.0
82-83	36.67329220610864	38.0	38.0	38.0	34.5	38.0
84-85	36.67125138060969	38.0	38.0	38.0	34.0	38.0
86-87	36.66065680327765	38.0	38.0	38.0	34.0	38.0
88-89	36.63598984672684	38.0	38.0	38.0	34.0	38.0
90-91	36.616901223346886	38.0	38.0	38.0	34.0	38.0
92-93	36.61832405884279	38.0	38.0	38.0	34.0	38.0
94-95	36.561019295687714	38.0	38.0	38.0	34.0	38.0
96-97	36.5264331225907	38.0	38.0	38.0	34.0	38.0
98-99	36.555667570795606	38.0	38.0	38.0	34.0	38.0
100	34.900205561237115	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	12.0
18	43.0
19	85.0
20	198.0
21	420.0
22	1237.0
23	3461.0
24	7863.0
25	15522.0
26	27084.0
27	43105.0
28	64402.0
29	92098.0
30	123913.0
31	161654.0
32	209846.0
33	280580.0
34	408122.0
35	704673.0
36	1949962.0
37	6977000.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.398389346128	18.355619223793454	14.515972859506759	27.730018570571787
2	13.2067049158146	19.381755196179686	44.65489866155682	22.756641226448888
3	16.8938370269743	26.776099963147892	30.854264366902473	25.475798642975338
4	11.71929532989862	18.869308697819946	38.94351872592871	30.467877246352725
5	13.217589176047303	37.19914470916155	35.99350149500662	13.589764619784525
6	29.13669344167381	38.597261199482986	18.829827111072174	13.43621824777103
7	26.023061470760382	33.0156404679495	22.865928781495906	18.09536927979421
8	23.968240347999508	36.863379844065	21.208396861067556	17.95998294686793
9	25.120853234675668	17.077889819424673	21.325926180170676	36.475330765728984
10-11	23.746400596859623	26.96643929157243	29.005101487813512	20.282058623754434
12-13	24.84468372220737	24.22331925486484	29.13325740113158	21.798739621796216
14-15	21.947787428373232	25.62570452558331	26.813923051354493	25.61258499468896
16-17	21.48828319760678	29.011225440960757	27.251424406211388	22.249066955221075
18-19	21.42909401622938	27.87158305092094	29.397305460615215	21.302017472234468
20-21	22.276092173980434	26.918130228530895	29.219896438540427	21.585881158948244
22-23	21.93584407981527	27.153009286682067	29.13405130155772	21.777095331944945
24-25	21.839771682357497	27.346914919554855	29.278654499192896	21.534658898894754
26-27	21.904947758738178	27.457132667440476	29.218155236017935	21.41976433780341
28-29	21.59802263098637	27.32041898576467	29.407778902243702	21.67377948100525
30-31	22.17701154364116	27.28786057624355	29.08430397237396	21.450823907741327
32-33	21.886329021528667	27.077526170885026	29.247696221320428	21.788448586265886
34-35	21.567711220469498	27.48697355949204	29.555664450753827	21.389650769284632
36-37	21.967139170560664	27.326342061715884	29.133824365600645	21.572694402122806
38-39	21.599994907721225	27.10399266711856	29.495925764833252	21.80008666032696
40-41	21.850343491601933	27.03499766518854	29.380219842179923	21.7344390010296
42-43	22.051453900733875	27.016433424711252	29.178514162186413	21.753598512368463
44-45	21.86537670743734	27.474167734387706	28.915660006618072	21.744795551556884
46-47	22.10891435704665	27.081834613478513	28.500185015126405	22.30906601434843
48-49	22.072324110506727	27.199333509392236	28.481648709291917	22.24669367080912
50-51	21.720237065080873	27.345130095435476	28.65847677139938	22.27615606808428
52-53	21.899879692372398	27.347299511116752	28.76750720946436	21.98531358704649
54-55	21.94043086225145	27.27210826965891	28.490551348637922	22.296909519451717
56-57	22.310090949564582	27.55829843698756	28.048015192547872	22.08359542089999
58-59	21.81313898706972	27.975421667971084	28.611845515236073	21.599593829723123
60-61	22.183643285186243	28.233790181143263	27.939371919307117	21.643194614363377
62-63	21.806052322262417	28.607102567926418	27.80739669925082	21.779448410560345
64-65	21.742025220097144	28.60386373512987	27.870134171531134	21.783976873241848
66-67	21.87264640829059	28.435705185101995	27.763052595345915	21.9285958112615
68-69	21.90034309521461	28.24892727632482	27.95861157746911	21.892118050991456
70-71	21.90519008762583	27.821976382880216	28.072421618274525	22.20041191121943
72-73	22.11202208396251	27.534650903332707	28.217447947985118	22.13587906471966
74-75	21.962863103092708	27.670699133620317	28.142673020736343	22.22376474255063
76-77	22.038283907231424	27.613793571028467	28.02558179453528	22.322340727204832
78-79	22.076537649551746	27.640925480540773	28.024686852480073	22.257850017427405
80-81	22.071578088189973	27.608197743104775	28.043099987275355	22.277124181429897
82-83	22.161095159626647	27.534453988767766	28.15841606297881	22.146034788626782
84-85	22.39055389282016	27.562744342176547	27.97551633226721	22.07118543273608
86-87	22.083138073405177	27.62976428351161	28.049225180600047	22.237872462483168
88-89	22.127178879265553	27.595266584164683	28.01122687389524	22.266327662674517
90-91	22.261895872220904	27.59653543296215	28.011651342906323	22.12991735191062
92-93	22.30206936472567	27.648901421838467	27.92886407255847	22.12016514087739
94-95	22.069951029871337	27.6976634504767	28.010202477450676	22.22218304220129
96-97	22.158461172308307	27.898477859142563	28.016343474909966	21.926717493639167
98-99	23.29886073814091	29.735372098945195	28.210550552671904	18.755216610241987
100	22.186782881222065	30.543685623737794	22.148406712494843	25.121124782545294
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	995.0
1	1148.5
2	2394.5
3	3745.0
4	5333.0
5	8499.0
6	10849.5
7	12442.0
8	14131.5
9	14756.0
10	14274.0
11	14183.5
12	16183.0
13	21197.0
14	30914.0
15	42524.5
16	51784.5
17	57202.0
18	56458.5
19	50350.0
20	43269.5
21	38159.5
22	37136.5
23	41518.5
24	50743.0
25	64924.0
26	85077.0
27	107584.5
28	129525.0
29	154398.0
30	178238.5
31	200784.5
32	227442.0
33	252761.0
34	278969.0
35	306159.0
36	333287.0
37	361105.0
38	378980.0
39	390473.5
40	408047.5
41	424285.5
42	438419.0
43	472518.0
44	502037.5
45	504875.5
46	493814.5
47	479758.5
48	469171.0
49	456048.5
50	444294.0
51	431141.5
52	413128.5
53	396586.0
54	376136.0
55	344380.0
56	308758.5
57	272028.0
58	235304.5
59	199850.0
60	163193.5
61	129671.0
62	100394.0
63	74324.0
64	53829.5
65	39099.5
66	28343.0
67	20918.0
68	15686.5
69	11522.5
70	8184.5
71	5803.0
72	4202.5
73	2778.5
74	1732.0
75	1062.0
76	620.0
77	365.0
78	230.5
79	145.5
80	99.5
81	65.5
82	44.5
83	30.0
84	20.5
85	15.0
86	13.5
87	11.0
88	7.5
89	3.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0032335917798122712
3	0.0
4	0.0
5	0.0038477935703911384
6	9.032379273218634E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.3549352160308708E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	1.3592615186539613E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	4.606513462627725E-6
42-43	0.0
44-45	0.0
46-47	1.4128682723569215E-5
48-49	4.037305654199071E-4
50-51	0.0013835546765488536
52-53	0.003732198822042829
54-55	0.005371174634613219
56-57	0.0062730701697212895
58-59	0.007791391892711547
60-61	0.007361530164379341
62-63	0.005296448538338045
64-65	0.003207905248481562
66-67	0.0011220356010675863
68-69	4.125397011448957E-4
70-71	2.4000444112565837E-4
72-73	5.180527370285541E-4
74-75	2.6666690844466366E-5
76-77	3.067093972046505E-4
78-79	2.498157337424808E-4
80-81	1.0416546739319339E-4
82-83	2.7674323469337317E-5
84-85	1.1730589254311018E-4
86-87	1.466033167421608E-4
88-89	2.8468583779056463E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	105.0
20-21	366.0
22-23	770.0
24-25	1023.0
26-27	2610.0
28-29	7245.0
30-31	18473.0
32-33	23080.0
34-35	31773.0
36-37	51183.0
38-39	64687.0
40-41	64881.0
42-43	74806.0
44-45	89933.0
46-47	91649.0
48-49	85214.0
50-51	76283.0
52-53	72609.0
54-55	76795.0
56-57	78800.0
58-59	79297.0
60-61	81265.0
62-63	80352.0
64-65	88955.0
66-67	104352.0
68-69	107850.0
70-71	135876.0
72-73	86087.0
74-75	81804.0
76-77	84619.0
78-79	86734.0
80-81	86946.0
82-83	83364.0
84-85	82994.0
86-87	85028.0
88-89	89409.0
90-91	90130.0
92-93	90903.0
94-95	108759.0
96-97	335744.0
98-99	771486.0
100-101	7317041.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.90197917202173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.39802082528149	68.16974839660259
2	8.4293532857982	13.30185314779325
3	2.6129655796187974	6.18504467220876
4	1.1067552126328177	3.4930070694272417
5	0.5410637738918269	2.1345501309174195
6	0.3054000525234602	1.4458001150004238
7	0.1857674604439301	1.026019420635044
8	0.11383119454642923	0.7185205232982969
9	0.07607673966346251	0.5402344795561653
>10	0.22709438479230193	2.5975836691871863
>50	0.002538560490786505	0.1325639266074525
>100	9.9757521609323E-4	0.13174111752094353
>500	9.023701377478754E-5	0.05510772621804946
>1k	4.511808675090429E-5	0.06822560502720662
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.032379273218633E-6	0.0	0.0	0.0
3	0.0	9.032379273218633E-6	0.0	0.0	0.0
4	0.0	9.032379273218633E-6	0.0	0.0	0.0
5	0.0	9.032379273218633E-6	0.0	0.0	0.0
6	2.348418611036845E-4	9.032379273218633E-6	0.0	0.0	0.0
7	2.348418611036845E-4	9.032379273218633E-6	0.0	3.6129517092874534E-5	0.0
8	2.4387424037690313E-4	9.032379273218633E-6	0.0	3.6129517092874534E-5	1.8064758546437267E-5
9	2.4387424037690313E-4	1.8064758546437267E-5	0.0	3.6129517092874534E-5	3.6129517092874534E-5
10-11	2.619389989233404E-4	1.8064758546437267E-5	0.0	3.6129517092874534E-5	3.6129517092874534E-5
12-13	2.7097137819655904E-4	1.8064758546437267E-5	0.0	3.6129517092874534E-5	3.6129517092874534E-5
14-15	2.800037574697777E-4	6.322665491253044E-5	0.0	3.6129517092874534E-5	5.41942756393118E-5
16-17	4.787161014805876E-4	6.322665491253044E-5	0.0	4.9678086002702485E-5	7.225903418574907E-5
18-19	4.787161014805876E-4	6.322665491253044E-5	0.0	7.225903418574907E-5	8.129141345896771E-5
20-21	4.787161014805876E-4	6.322665491253044E-5	0.0	9.935617200540497E-5	8.129141345896771E-5
22-23	4.922646703904156E-4	7.677522382235839E-5	0.0	1.0838855127862361E-4	9.935617200540497E-5
24-25	4.967808600270249E-4	9.483998236879565E-5	0.0	1.4000187873488884E-4	1.4000187873488884E-4
26-27	4.967808600270249E-4	1.0387236164201429E-4	0.0	1.7161520619115404E-4	1.4451806837149813E-4
28-29	5.058132393002435E-4	1.0838855127862361E-4	0.0	3.116170849260429E-4	1.4451806837149813E-4
30-31	5.058132393002435E-4	1.1742093055184224E-4	0.0	7.677522382235839E-4	1.4903425800810746E-4
32-33	5.058132393002435E-4	1.2645330982506087E-4	0.0	0.0017703463375508524	1.6258282691793542E-4
34-35	5.193618082100714E-4	1.4000187873488884E-4	0.0	0.0030981060907139917	1.7161520619115404E-4
36-37	5.238779978466808E-4	1.5355044764471678E-4	0.0	0.005189101892464105	1.7161520619115404E-4
38-39	5.238779978466808E-4	1.6258282691793542E-4	0.0	0.00859882506810414	1.8064758546437268E-4
40-41	5.329103771198994E-4	1.8064758546437268E-4	0.0	0.012595652896503384	1.8064758546437268E-4
42-43	5.329103771198994E-4	1.9871234401080994E-4	0.0	0.01647054360471418	1.8064758546437268E-4
44-45	5.329103771198994E-4	2.4839043001351245E-4	0.0	0.02063898663930458	1.8064758546437268E-4
46-47	5.329103771198994E-4	2.800037574697777E-4	0.0	0.025471309550476547	1.896799647375913E-4
48-49	5.419427563931181E-4	2.800037574697777E-4	0.0	0.03021330866891633	2.0774472328402858E-4
50-51	5.464589460297273E-4	3.025847056528242E-4	0.0	0.03500046968372221	2.0774472328402858E-4
52-53	5.509751356663367E-4	3.116170849260429E-4	0.0	0.03948052980323865	2.1677710255724723E-4
54-55	5.509751356663367E-4	3.161332745626522E-4	0.0	0.04419543178385878	2.3032567146707517E-4
56-57	5.55491325302946E-4	3.161332745626522E-4	0.0	0.048978076609028046	2.348418611036845E-4
58-59	5.600075149395554E-4	3.206494641992615E-4	0.0	0.05368846240001156	2.348418611036845E-4
60-61	6.27750359488695E-4	3.2516565383587084E-4	0.0	0.058457558656271	2.348418611036845E-4
62-63	7.045255833110534E-4	3.341980331090895E-4	0.0	0.06271180929395698	2.5290661965012175E-4
64-65	7.090417729476628E-4	3.522627916555267E-4	0.0	0.0670789646725582	2.5290661965012175E-4
66-67	7.451712900405374E-4	3.6129517092874536E-4	0.0	0.0719293523422766	2.5742280928673104E-4
68-69	7.587198589503653E-4	3.6129517092874536E-4	0.0	0.07649522006488861	2.619389989233404E-4
70-71	7.677522382235839E-4	3.6129517092874536E-4	0.0	0.08072688975439155	2.619389989233404E-4
72-73	8.400112724093329E-4	3.6129517092874536E-4	0.0	0.08387467393110823	3.432304123823081E-4
74-75	8.400112724093329E-4	3.6581136056535466E-4	0.0	0.08641728869651928	3.432304123823081E-4
76-77	8.490436516825516E-4	3.929084983850106E-4	0.0	0.08878828825573917	3.4774660201891737E-4
78-79	8.490436516825516E-4	4.064570672948385E-4	0.0	0.09034185749073279	3.522627916555267E-4
80-81	8.490436516825516E-4	4.064570672948385E-4	0.0	0.09081605740257676	3.70327550201964E-4
82-83	8.490436516825516E-4	4.064570672948385E-4	0.0	0.09126316017660108	4.0194087765822923E-4
84-85	8.490436516825516E-4	4.064570672948385E-4	0.0	0.09150703441697798	4.064570672948385E-4
86-87	8.490436516825516E-4	4.1548944656805717E-4	0.0	0.09161090677862	4.064570672948385E-4
88	8.490436516825516E-4	4.516189636609317E-4	0.0	0.0916154229682566	4.1548944656805717E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	11390	0.0	48.893078	1
GGTATCA	14450	0.0	47.663074	1
GTATCAA	28770	0.0	41.004658	1
TATACAC	16370	0.0	36.633083	3
ATCAACG	34645	0.0	34.06279	3
TCAACGC	35175	0.0	33.561718	4
CAACGCA	35435	0.0	33.279224	5
TATCAAC	36710	0.0	32.520554	2
TTATACA	17720	0.0	32.34526	2
AACGCAG	36895	0.0	32.054108	6
ACGCAGA	41145	0.0	28.711794	7
CGCAGAG	41885	0.0	28.163654	8
ACACATC	20970	0.0	26.820524	6
ACATCTC	21235	0.0	26.626795	8
TACACAT	23005	0.0	26.17917	5
GCAGAGT	48385	0.0	24.424406	9
CACATCT	22890	0.0	24.421127	7
ATACACA	26960	0.0	23.720003	4
CATCTCC	24230	0.0	22.964565	9
TATGCCG	14630	0.0	22.35663	42-43
>>END_MODULE